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All Journal JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Akademika Biologi Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Berkala Bioteknologi Media Farmasi Indonesia
Endang Kusdiyantini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Other

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KARAKTERISTIK MORFOLOGI, BIOKIMIA, DAN MOLEKULER ISOLAT KHAMIR IK-2 HASIL ISOLASI DARI JUS BUAH SIRSAK (Annona muricata L.) Vivi Suryaningsih; Rejeki Siti Ferniah; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Yeast was found in foods that contain lots of sugar as fruits. Yeast utilizes simple sugars in food to gain energy. Yeast has a role in the natural fermentation of the fruit that resulting of decay, discoloration, and cause the fruit not durable. The purpose of this research were to isolate the yeast from the soursop fruit and to identify the yeast in morphology, biochemistry, and molecular test based on Internal Transcribed Spacer (ITS). Isolation of  yeast  was  performed by growing on  medium PDA and chloramphenicol. Identification was done through biochemical test by looking at the capabilities in the fermentation of carbohydrate and the abilities to grow on a medium to high osmotic pressure. Molecular identification was done using Internal Transcribed Spacer (ITS) region. The result showed that isolate IK-2 yeast from the soursop fruit juice had a distinctive form round to oval, prominent elevation, the colour creamy white, form a buds, able to ferment glucose and sucrose, but not able to ferment lactose, as well as being able to grow on media with glucose level 50 %. Molecular analysis of the ITS region using ITS1 and ITS4 primers, and phylogenetic analysis using Neighbor Joining. The result of the Basic Local Alignment Tools (BLAST) showed that the isolate had 95% homology with Candida tropicalis. Key Words : Yeast, Soursop (Annona muricata L.), Internal Transcribed Spacer (ITS)
ISOLASI, KARAKTERISASI BAKTERI ASAM LAKTAT, DAN ANALISIS PROKSIMAT DARI PANGAN FERMENTASI RUSIP IKAN TERI (Stolephorus sp.) Deverina Mergypta; Anto Budiharjo; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Rusip is a traditional food from Bangka-Belitung made from fresh anchovy with salt and palm sugar that is brooded for seven days. Rusip fermentation process is caused by microbial activity mainly from lactic acid bacteria. The purpose of this study was to isolate lactic acid bacteria from rusip, to characterize isolates obtained based on common characteristic of lactic acid bacteria, and to carry out proximate analysis of rusip anchovy. Isolation from rusip fermented food obtained eight bacterial isolates, five isolates were circular and three isolates were rod-shaped. Characterization and identification of lactic acid bacterial were performed using tests that led to the common characteristic of lactic acid bacteria, i.e the gram staining, motility test, acid formation test, catalase test, and proteolytic test. The result showed that eight bacterial isolates were gram-positive, non-motile, catalase-negative, positive to produce acid, and had proteolytic activity. Rusip anchovy had 0,64% of total lactic acid and the pH 5,40. Nutrient content of rusip fermentation had 10,3644% moisture content; 50,0702% ash content; 0,9524% crude fat content; 0,3764% crude fiber content; 34,8603% crude protein content; and 14,1171% carbohydrate content. Keywords: rusip, isolation, characterization, lactic acid bacteria, proximate analysis
Fermentasi Kopi Robusta (Coffea canephora) Menggunakan Isolat Bakteri Asam Laktat Dari Feces Luwak Dengan Perlakuan Lama Waktu Inkubasi Doni Usman; Agung Suprihadi; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Coffee is one of Indonesian main corps commodity. Caffeine content of robusta coffee is 1.6%-2.4% higher than arabica coffee that was 0.9-1.2%. Coffee fermentation was one of method to decrease caffeine content. Luwak coffee is coffee that produces by luwak use their digest system in luwak body and defecates as faeces. Lactic acid bacteria (LAB) exploration from luwak faeces that fed by robusta coffee is one of method to get lactic acid bacteria. This research aimed was lactic acid bacteria isolation from luwak faeces and determine best incubation time on robusta coffee fermentation. Bacteria isolation was resulted 27 isolate. Biochemical test done by Gram Staining Test, Catalase Test, Motility Test, and Acid Producing Test and resulted 13 LAB isolate. Characterization test done to all of LAB isolate and resulted best 3 isolate. Isolate M6 was xylanolitic potency, M8 was Proteolytic potentcy and m16 was cellulolytic potency. Robusta coffee fermentation done used mixed of M8, M6 and M16 as inoculum. This research use Complete Random Design (RAL), the treatment was fermentation time 8 hour (D1), 16 hour (D2) and 24 hour (D3), each treatment replays 3 times. Kontrol of this fermentation is robusta coffee without fermentation treatment (control 1) and luwak robusta coffee  (control 2). Data analysed by Analysis of Variance (ANOVA) continuing by Duncan Test with significance rate was 95%. This research result shown that fermentation time duration was cause decrease of caffein content, coffee beans weight and fermentation fluid pH. Highest caffeine content on D1 was 0.95%, and D3 was 0.72 %.Keyword: Luwak faeces, lactic acid bacteria, gram staining, fermentation, exploration
KARAKTERISASI MOLEKULER TANAMAN SAMBUNG NYAWA (Gynura procumbens [Lour.] Merr) BERDASARKAN 18S rRNA Octavia Dewi Christiningrum; Anto Budiharjo; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Sambung Nyawa (Gynura procumbens) is a traditional medicinal plant used by people as an antihyperglycemic drug, antihyperlipidemic and chemoprevention agents. Comprehensive information on this plant needs to be done, including DNA analysis, so it can complement morphological character of an organisms. This study aimed to molecularly characterize and determine the relationship between G. procumbens with other organisms using ribosomal 18S rRNA gene. PCR (Polymerase Chain Reaction) was used to amplify the 18S rRNA gene fragments G. procumbens. DNA sequences were analyzed using the BLAST program at the NCBI portal and construction of phylogenetic trees were tested using Bootstrap method 1000x. The results showed the 18S rRNA sequence of Gynura procumbens fragments was 1600 bp, and G. procumbens belonged in the family of Asteraceae.Key words : Gynura procumbens, PCR (Polymerase Chain Raction), 18S rRNA, Bootstrap method
KEMAMPUAN ISOLAT BAKTERI PENGHASIL INDOLE ACETIC ACID (IAA) DARI TANAH GAMBUT SAMPIT KALIMANTAN TENGAH Sofyan Fauzi Larosa; Endang Kusdiyantini; Budi Raharjo; Antonius Sarjiya
Jurnal Akademika Biologi Vol. 2 No. 3 Juli 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Peat soil is present in many areas of Indonesia, one of them is in Sampit regency Central Kalimantan. Peat soil is a type of soil which has an extreme characteristic  of pH  around 3-5. However, it is presumed that there is bacteria that can produce growth hormones, such as  IAA. This study aimed to determine the ability of bacterial  isolated from peat garden and burned area in Sampit regency which could  produce IAA. Several tests were carried out including bacterial isolation, purification isolates, growth at different pH conditions, as well as IAA production with the addition of L-Tryptophan precursors with different concentrations. The  growth and concentration of IAA was measured  using spectrophotometry with wavelength 600 nm to 530 nm for growth and IAA, respectively. Results showed that  five isolates produced IAA, three isolates from peat garden area and two isolates from burned areas. All isolates showed the highest growth at pH 6, and the highest IAA production  at  43.311 ppm was produced by TGK6  isolate with the addition of 300 ppm L-Tryptophan. Keywords: Indole Acetic Acid, Peat Soil, ­L-Tryptophan
Ekstraksi Protein dari Escherichia coli BL21 Rekombinan Gen Mycobacterium tuberculosis dengan Variasi Waktu Inkubasi Induksi Isoprophyl-β-D-Thiogalactosidase (IPTG) dan Metode Lisis Sel Nabila Swarna Puspa Hermana; Endang Kusdiyantini; Agung Suprihadi; Neny Nuraini
Jurnal Akademika Biologi Vol. 4 No. 2 April 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bacillus Calmete-Guerin vaccine is no more effective in preventing tuberculosis. The vaccine developed now is vaccine recombinant using Mycobacterium tuberculosis gen cloned in Escherchia coli to produce protein which is used as antigen compound in vaccine. The protein produced an intercellular protein which is needed cell lysis process so that the protein could be extracted. The aim of this research was to investigate the incubation time, and the optimum cell lysis method in protein recombinant extraction. Recombinant E. coli BL21 was incubated for 4, 8, 12, and 16 hours, and followed by, freeze thaw, sonication, and beads vortex lysis method. SDS PAGE quantification showed that the sample with 12 hour incubation with freeze thawing lysis method had the highest protein recombinant concentration by 4.327.870,4 pixel. Keywords: E. coli BL21 recombinant, protein extraction, enzymatic, freeze thaw, sonication, beads vortex
Kandungan Proksimat dan Daya Pengikatan Tepung Iles-Iles (Amorphophallus oncophylus) Terhadap Aflatoksin Sebagai Upaya Pencarian Bahan Pengikat Alternatif pada Pakan Sigit Hananto; Sri Pujiyanto; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 4 No. 4 Oktober 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Aflatoxin  is  a  metabolite  secondary  of  Aspergillus  flavus  and  A.  Parasiticus. Contamination  aflatoxin in poultry have an impact on their liver damage. Technique to reduce the effects of aflatoksin can be done by addition of aflatoxin binder. Aflatoxin binder material commonly used still import as  glucomannan yeast product (GYP), so we needed study local material that has the potential  as a a  aflatoxin binder. The purposes is  test proximate content and the binding power of flour iles-iles compared  to GYP. Sample in research  is  flour  derived  from  bulbs  iles-iles  (Amorphophalus  oncophylus)  and  GYP  as control. The parameters in research that is the physical characteristics , proximate analysis , glukomannan levels and the ability of binding aflatoksin in vitro between flour iles-iles and GYP. The results of the physical test, the sample has delicate texture and gross energy flour iles-iles not significantly different with GYP. The results of proximate analysis ( the water level , ashes , crude fiber , crude protein and crude fat) show between flour iles-iles and GYP was significantly different, except crude fat that shown the result is no different (p<0,05) .The results of the analysis in the glukomannan levels of flour iles-iles is 52,74 % and GYP 17,36%. Testing the ability bind done with a solution of aflatoksin 2 ml (2.7 ng/ml) and materials binder used is 0  g; 0,0013 g; 0,0027 g and 0,0040 g. The research results show binding aflatoxin by flour iles-iles optimal in heavy 0,0013 g (92,92 %), while binding aflatoxin by GYP optimal in heavy 0,0040 g (87,71 %). The ability binding aflatoksin flour iles-iles in proportion to GYP so that can be used as a alternative binder in feed. Keyword: Iles-iles (Amorphophallus oncophylus), aflatoxin, Glucomannan 
ANALISIS KANDUNGAN β-KAROTEN FUSAN INTRASPESIES Chlorella vulgaris dan APLIKASINYA SEBAGAI PAKAN TAMBAHAN PADA POST LARVA STADIA 10 UDANG WINDU (Penaeus monodon). Dwi Kristyaningrum; Hermin Pancasakti Kusumaningrum; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 2 No. 3 Juli 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Carotenoids are one of pigment which needed for development of post-larvae tiger shrimp (Penaeus monodon), but the shrimp unable to synthesis carotenoids by themselves in the body so it takes a feed containing carotenoids. Carotenoids can be obtained from synthetic feed or natural feed. One of the natural feed is microalgae Chlorella vulgaris. β-carotene is the highest content of carotenoid in C. vulgaris, this content can be improved by protoplast fusion. The purpose of this study was to determine the amount of β-carotene in fusan intraspecies C. vulgaris and it’s application as supplement in the post-larvae stadia 10th tiger shrimp (P. monodon) Measurement of the amount of  β-carotene using UV-Vis at a wavelength of 460nm. The feeding is done three times a day. The results showed that the amount of β-carotene on fusan C. vulgaris is greater than the wildtype of C. vulgaris. β-carotene in the fusan C. vulgaris is14.88µg/g while the wildtype of C. vulgaris is 7.14 µg/g. Additional applications C. vulgaris by 10 x 104 in 0.0225 g synthetys feed post-larvae stadia 10th shrimp (P. monodon) compared to the post-larvae shrimp fed only control showed that the feed given additional fusan of C. vulgaris increases the weight of shrimp at 39.9%, while the given wildtype of C. vulgaris increasing shrimp weight by 10.1%. Keywords: β-carotene, Chlorella vulgaris, protoplast fusion, post larvae shrimp
Optimasi Linamarase pada Umbi Singkong (Manihot esculenta Crantz) dan Umbi Gadung (Dioscorea hipsida Dennst) dengan Variasi Suhu dan pH yang Berbeda Yoni Anggun Endah Kurniati; W Wijanarka; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 4 No. 4 Oktober 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Singkong (Manihot esculenta Crantz) dan gadung (Dioscorea hipsida Dennst) merupakan bahan pangan yang memiliki kandungan karbohidrat tinggi. Singkong dan gadung sering dikonsumsi oleh masyarakat Indonesia sebagai pengganti nasi. Kedua bahan pangan ini berpotensi menyebabkan keracunan sianida apabila tidak diolah dengan baik. Umbi singkong dan gadung mengandung enzim hidrolitik yaitu linamarase (EC 3.2.1.2.1) dan substrat linamarin. Senyawa sianida dihasilkan akibat adanya hidrolisis linamarase dengan linamarin pada kondisi pH>6. Penelitian ini bertujuan untuk mengetahui kondisi optimal linamarase dari umbi singkong dan gadung. Parameter yang diukur adalah kadar sianida menggunakan spektrofotometri λ= 510 nm. Penelitian ini dilakukan secara eksperimental menggunakan Rancangan Acak Kelompok (RAK) pola faktorial. Faktor pertama optimasi linamarase meliputi suhu 350C, 400C, dan 450C, sedangkan faktor kedua meliputi pH 6.5, 7.0, dan 7.5. Hasil penelitian menunjukkan bahwa kondisi optimal linamarase singkong pada suhu 450C dan pH 7,5. Kondisi optimal linamarase gadung  pada suhu 350C dan  pH 7.5.Kata kunci : Singkong (Manihot esculenta Crantz), Gadung (Dioscorea hipsida Dennst), Linamarase, Linamarin, Sianida 
ISOLASI, KARAKTERISASI BAKTERI ASAM LAKTAT, DAN ANALISIS PROKSIMAT DARI MAKANAN FERMENTASI BEKASAM IKAN MUJAIR (Oreochromis mossambicus Peters) Olivia Nisa Mumtianah; Endang Kusdiyantini; Anto Budiharjo
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bekasam is one of the traditional fermented food made from freshwater fish with the addition of salt and carbohydrate sources. Mozambique fish is used as a raw material, because it has high protein content and can be easily obtained in the market at affordable prices. Mozambique fish is used to make bekasam with the addition of salt as much as 18% of the weight of the fish and brooded for 48 hours, then added with toasted rice as much as 15% of the weight of the fish and fermented for 7 days. This study aimed to isolate and characterize lactic acid bacteria and analyze the nutritional value of mozambique fish bekasam. The methods used were  isolation, characterization and proximate analysis of the mozambique fish bekasam. Isolation of lactic acid bacteria was carried out on mozambique fish as a control, mozambique fish before and after the addition of toasted rice. Isolation obtained 6 isolates of Gram positive bacteria, 5 isolates of cocci shaped bacteria and 1 rod shaped bacteria. Based on morphological and physiological tests, six isolates were Gram positive, non motile, catalase negative, positive to produce acid and had proteolytic activity. Bekasam mozambique fish contained as much as 0.64% lactic acid and pH is 5.39. The results of the proximate analysis bekasam mozambique fish showed that the fermentation process increased the nutritional value bekasam quality mozambique fish with 5.5270% water content, 33.0628% ash content, 0.0788% crude fiber content, 45.0546% crude protein content, 7.9419% crude fat content and 13.9407% carbohydrate content. Keywords : bekasam , lactic acid bacteria , isolation, characterization, proximate analysis
Co-Authors A Heru Prianto AB SUSANTO Agung Suprihadi Ahmad Qi Sahlan Aninditia Sabdaningsih Aniza Rachmawati Anto Budiharjo Antonius Sarjiya Arina Aisyah Arina Tri Lunggani Barri Pratama Betalini Hapsari Bintoro Rudi Saputro Budi Raharjo Choirul Rizka Putri Dani Ika Purwaningsih Darnia Astari Parastiti Desi Arisanti Deverina Mergypta Devi Ayu Ningsih Devia Kusmawati Arfina Doni Usman Dwi Kristyaningrum Dwina Mulyaningtyas Dyah Palupi Endah Dwi Hastuti Erma Prihastanti Fauziah Citra Rahmawati Hadi Endrawati Hermin Hermin Hermin Pancasakti Kusumaningrum Ika Oktavia Nurmila Indah Sulistyarini JOEDORO SOEDARSONO Khrisna Lazuardi Budi Laila Nur Faizah Lasria Pardede Linda Safitri Luthfy AN M Eka Prastya Mangasa Tua Pandapotan Siregar MG Isworo Rukmi Mia Tri Wardani Muhamad Fikri Zulfikar Muhammad Zaenuri Nabila Swarna Puspa Hermana Neny Nuraini Octavia Dewi Christiningrum Olivia Nisa Mumtianah Rejeki Siti Ferniah Risky Panji Nugroho Rully Rahadian Sari, Anisa Rachma Setiawan Wicaksono Sigit Hananto Siti Nur Jannah Sofyan Fauzi Larosa Sona Fatimah Soni Nugraha Anwar Sri Pujiyanto Susiana Purwantisari Syawal Nurangga Kodhatin T. A. Lestari Tri Muji Ermayanti Triwibowo Yuwono Vivi Suryaningsih w wijanarka Wijanarka Wijanarka Yadi Suryadi Yeti Darmayati Yoni Anggun Endah Kurniati