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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences International Journal of Applied Biology
Dominggus Rumahlatu
Biology Education Study Program, Faculty of Teacher Training and Education Science, Pattimura University
Agriculture, Biological Sciences & Forestry Automotive Engineering Earth & Planetary Sciences Energy Environmental Science

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Isolation and Identification of Protease Enzyme Producing Bacteria from Fermentation of Gonad Sea Urchin (Echinothrix calamaris) Siani La Jamaludin; Johanis Fritzgal Rehena; Cecilia Anna Seumahu; Dominggus Rumahlatu
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 23, No 4 (2018): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (652.56 KB) | DOI: 10.14710/ik.ijms.23.4.187-198

Abstract

Bekasang of gonad sea urchin is one of the traditional fermentation products which generally involves microorganism spontaneous fermentation. Fermented paste products have a long shelf life and are processed quite easily using protease enzymes. Good exploration of producing protease from bakasang is needed to obtain the protease enzyme-producing microorganism with different characters. The method used in this research is screening with clear zone, measuring the activity of crude extract of protease enzyme characterization of bacteria through gram staining. Identification of potential microorganisms through 16S rRNA sequence. The results showed that there were eight isolates of protease enzyme-producing bacteria (G1, G2, G3, G4, G5, G6, G7, and G8) indicated by clear zones around single-colonic bacterial streaks. Only five bacterial isolates (G1, G4, G6, G7, and G8) were tested for the enzyme activity. These isolates have characteristics of positive gram bacteria. The interpretation of the results of molecular analysis using PCR and BLASTN sequences of 16S rRNA gene from five bacterial isolates, showed the identity of bacteria as: G1 was Staphylococcus piscifermentans strain CIP103958 with 99% similarity; Isolate G4 was Staphylococcus saprophyticus strain ATCC 15305 with 99% similarity; Isolate G6 was Staphylococcus condimenti F-2 strain with 99% similarity; Isolate G7 was Bacillus amyloliquefaciens subsp. plantarum strain FZB42 with 99% similarity; And G8 isolates was Lactobacillus plantarum strain JCM 1149 with 99% similarity.
Macrozoobenthos Community Structure in the Coastal Waters of Marsegu Island, Maluku, Indonesia Fitry Yunita; Fredy Leiwakabessy; Dominggus Rumahlatu
International Journal of Applied Biology Vol. 2 No. 1 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v2i1.3224

Abstract

The existence of macrozoobenthos in an ecosystem is closely related to the conditions of the surrounding environment. This research aimed to examine the physical chemical condition of aquatic environment and macrozoobenthos community structure in the coastal waters of Marsergu Island. This research was conducted in July and December 2016 in 3 stations. The data were collected by using sampling technique to measure the environmental factors (temperature, salinity, pH, and dissolved oxygen of seawater) and enumeration of gastropod species with in-situ. The results of this research showed that macrozoobenthos in the coastal waters of Marsegu Island consisted of 2 Phylum, namely Molusca and Echinoderms, that is, gastropod with 18 species, bivalvia with 5 species, and echinoderms with 17 species. Echinoderms was the dominant macrozoobentos with a percentage of 45%, followed by gastropods 42%, and bivalvia 13%. This suggests that echinoderms and gastropods are the main components that make up the macrozoobenthos community structure in the coastal waters of Marsegu Island. The results of the calculation of the diversity index of macrozoobenthos in the three research stations showed the highest score was station 1 as much as 3.56, station 2 as much as 3.52 and the lowest was station 3 as much as 2.07.
Co-Authors CECILIA ANNA SEUMAHU Fitry Yunita Fredy Leiwakabessy Johanis Fritzgal Rehena Siani La Jamaludin