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All Journal Buletin Peternakan Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education

Atit Kanti

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Author-ID : 295442

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Carboxymethyl Cellulose Hydrolyzing Yeast Isolated from South East Sulawesi, Indonesia Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2203

ABSTRACTThe objective of study was to isolate, identify and characterize the CMC-ase producing yeast from South East Sulawesi, Indonesia.Â We isolated 142 strains and obtain 53 strains (37.32%) were CMC-ase producer consist of 26 species residing within 10 genera.Â Candida was the most diverse genus consisting of 15 species. It is important to note that several strains residing within this genus could be candidate for new taxa, among others Candida aff. cylindracea PL2W1, Candida aff. insectorum PL3W6, Candida aff. friedrichiiÂ MKL7W3, Candida aff. lessepsii, Candida aff. tenuis. Â Five new candidates for novel species of cellulolytic yeast close to Yamadazyma mexicana: were Yamadazyma aff. mexicana (5 strains). Pichia, Pseudozyma, Sporodiobolus, and Sporobolomyces were other cellulolytic yeasts found in South East Sulawesi.Â It is obvious, that leaf litter was a good source for cellulolytic yeasts. This CMC-ase producing yeasts dominate this biome, and production of extracellular cellulase is critical strategy for such yeast to survive in cellulose rich ecosystem such as leaf-litter.Â This finding would suggest that yeasts play key role on hydrolyzes of cellulose and important resources for sustainable energy research.Â Keywords: cellulolytic yeasts, secondary forest, South East Sulawesi, leaf-litterÂ

Aktivitas Enzim Selulase dari Khamir Candida sp. dan Debaryomyces sp. yang Diisolasi dari Lahan gambut Taman Nasional Bukit Duabelas Jambi Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 4, No 1 (2006): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTCellulalytic Activity of Candida sp and Debaryomyces sp Isolated from Peat Soil ofBukit Duabelas Natural Park, Jambi. Candida sp. and Debaryomyces sp. YeastDegrading cellulose Isolated from Peat Soil in Bukit Duabelas National Park, Jambi. Theobjective of study was to investigate the characteristic of yeast that are able to solubilizecellulose. The yeast was isolated from soil of Tarnan Nasional Bukit Duabelas Jambi.Out of 2 1 ioslates tested 2 isolates Candida sp. (Isolate J I) and Debaryomyces sp. (IsolateJ2) were able to solubilize Carboxymethyl cellulose. Other isolated soil yeasts wereRhodotor& sp, and C~ptococcusp . Medium acidity during cell cultivation variedbetween 5.8 to 7.2. The CMC-ase activity was 5.9 unit and 5.4 unit for isolate J1 andisolate 52 respectively. The Km of isolate J1 and isolate 52 were 7.7 x 10" and 8.4 x 1 0-2(% b/v) respectively. Vmaks of isolate J1 and J2 was 8.28 xlW3 dan 30.66 x lo-) pgglukosa/ml enzyme /minute, respectively.Key words: Cellulolytic Yeasts, Candida sp., Debaryomyces sp., Enzymes Activity,Taman Nasional Bukit Duabelas

Keragaman Jenis Khamir Penghasil Etanol yang Diisolasi dari Makanan Fermentasi di Kepulauan Riau Sumerta, I Nyoman; Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTInformation on genetic diversity of fermentative yeast which produce ethanol is very crucial in developing biofuel production in Indonesia. Research on ethanol producing yeasts is interest of many scientist. The objective of study was to reveal yeast diversity in Indonesian fermented foods that able to produce ethanol. The sample of fermented foods were collected in the traditional market in Karimun Besar Island, Kepulauan Riau. Yeast isolation was performed using serial dilution with direct plating and enrichment culture with glucose as carbon source. Fifteen of isolates were isolated and identified by amplification of D1/D2 region LSU 26S rDNA. Its ethanol production characteristic was analyzed base on fermentation activity and measurement with gas chromatography for ethanol content. The result revealed that 8 yeast species were found belong to Ascomycetous and grouped into 5 clades which are able to produce ethanol. The highest ethanol production was obtained by Saccharomyces cerevisiae Y15Kr107 (3.53%) followed by Torulaspora delbrueckii Y15Kr104 (1.63%), Saccharomyces cerevisiae Y15Kr093 (1.58%), Candida glabrata Y15Kr110 (1.4%), Torulaspora delbrueckii Y15Kr103 (1.29%), Candida glabrata Y15Kr108 (1%), Torulaspora globosa Y15Kr094 (0.92%), Kodamaea ohmeri Y15Kr096 (0.61%), and Pichia kudriavsevii Y15Kr106 (0.31%) Y15Kr105 (0.21%) Y15Kr109 (0.16%). Other yeasts strains did not produce ethanol but may play different role in fermentation process.Key words: yeast, fermented food, ethanol, Kepulauan Riau

Pseudozyma aphidis as inoculant for local chicken Rahmansyah, Maman; Sugiharto, Arwan; Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

SHORT COMMUNICATION

Effect of Nitrogen Addition on the ?-Amylase Production by Aspergillus niger, Rhizopus oligosporus and Neurospora crassa in media contained Sargassum and Rice Seed on Solid State Fermentation Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTAmylase is one of the hydrolytic enzymes which is commercially important and represent about 25â33% of the world enzyme market. Amylase production is affected by substrate for enzyme productions include particle size, initial moisture content and nutrient addition. The present study describe the effect of N-species addition on alpha amylase production by Aspergillus niger Km1, Rhizopus oligosporus Km2 and Neurospora crassa Km3 in medium contained sargasum and rice seed on solid state fermentation. Various ration of media composition contained dried sargassum and rice seed were studied. The effect of particle size of sargassum, initial water content on ?-amylase production were evaluated. The best media composition was then augmented with N-species include sodium nitrate, yeast extract, and peptone on solid state fermentation. Best media composition was 60:40 (w/w) of sargassum and rice seed respectively, with initial moisture content was 60%. Increase 5 times of amylase activity was obtained when sodium nitrate (0,5% w/w) was added to production medium. N-species significantly affect Alpha amylase production on sargassum and rice seed with maximum alpha amylase production was 36,66 unit by Rhizopus oligosporus.The economic value of Sargassum can be increased through alpha amilase production.Keywords: Amylase, Nitrogen, solid state fermentation, Rhizopus oligosporus, Neurospora crassa

Identifikasi Keragaman Khamir yang Diisolasi dari Tanah Kebun Biologi Wamena Kabupaten Jayawijaya, Propinsi Papua Kanti, Atit; Latupapua, H.J.D.
JURNAL BIOLOGI INDONESIA Vol 3, No 2 (2001): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTThe Identification of Yeasts Which Were Isolated from Soils in the Wamena Biological Garden, Jayawijaya Residence, Papua Province. The present study deals with the isolation and identification of yeasts isolated from soil samples in The Wamena Biological Garden, Papua. Microbial population in 11 samples were estimated by plate count method. The yeasts population were ranged from 30.0 x102 until 16.5 x106 cells/g soil. Fifteen isolates of yeasts were taxonomically studied. On the basis of morphological and physiological characteristics these isolates were belonging to two group namely ascomycetous-imperfect yeasts and basidiomycetous yeasts. Futher, they were separated into three group. Out of 15 isolates, eleven isolates were included in group I and identified into genus level as Cryptococcus, three in group II as Candida and one isolate in group III identified into species level as Cryptococcus albidus. Type of sample sources appeared has no effect on yeasts diversity as shown by similar yeast isolate was observed isolated from different sources.Key words : Ascomycetous-imperfect yeasts, basidiomycetous yeasts, The Wamena Biological Garden, Papua.

C0-CULTURE OF AMYLOLYTIC FUNGI Aspergillus niger AND OLEAGINOUS YEAST Candida orthopsilosis ON CASSAVA WASTE FOR LIPID ACCUMULATION [Akumulasi lipid oleh kultur campuran kapang Aspergillus niger dan khamir Candida orthopsilosis pada media limbah singkong] Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The objective of study was to evaluate the effectiveness of co-culture between amylolytic fungiÂ Aspergillus niger and lipid accumulating yeast Candida orthopsilosis for lipid accumulation on cassava waste. When grown in 5 % starch medium, all microbes was able to grow, but best growth was observed on Aspergillus niger, other microbes grew slower. Moderate growth was observed Â on Saccharomycopsis fibuliger.Â The two isolates were then selected for further studies. Aspergillus niger and Saccharomycopsis fibuliger were amylolytic microbes. The amylolytic activities were affected by temperature, pH and nitrogen sources.Â Optimum temperature and pH for enzyme production were 30Â°C and 7.0 respectively. Both yeast extract and sodium nitrate were good nitrogen sources for amylase production. On cassava waste, the highest biomass and total lipid content were obtained by co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis. Major lipid composition was oleic acids and strearic acids. The ability of co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis grew and accumulated lipid on cassava waste would suggest that these culture were candidate for biofuel production.

DIVERSITY OF XYLOSE ASSIMILATING YEAST FROM THE ISLAND OF ENGGANO, SUMATERA, INDONESIA [Keragaman Khamir Pengguna Xilose yang Diisolasi dari Pulau Enggano, Sumatera, Indonesia] Kanti, Atit; Sumerta, I Nyoman
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Naturally occurring yeasts (wild type) are important genetic resources for many industrial interests. Though biodiversity exploration of Enggano has been done since 1944, but there is no report for yeast diversity from this island. Due to its industrial interest, xylose fermenting yeast is target for many scientists. This present study aims to isolate xylose assimilating yeast from island of Enggano. The samples were collected from varying sources included soil, leaf litter, decay wood, fruit and sediment. Xylose enrichment culture technique was performed to enrich xylose fermenting yeast. While for the isolation of yeast several methods which include dilution, ballistopore falling, direct isolation, membrane filtration were done. Of 200 isolates, 76 strains (38 %) were xylose assimilating yeast which mostly belong to Candida which include C. insectorum, C. tropicalis, C. boidinii, C. pseudolambica, C. yuanshanica, C. silvae, Cyberlindnera saturnus, Williopsis saturnus and Sporobolomyces poonsookiae. One strain Candida sp. that was isolated from soil could be assigned as a candidate of novel species on base on its morphological and biochemical characteristics along with analyses of gene sequence from D1/D2 domain. This study confirms that yeast survey is very important to obtain new genetic resources for industrial interest as well as for taxonomic study.

KEANEKARAGAMAN KHAMIR YANG DIISOLASI DARI SUMBER DAYA ALAM PULAU ENGGANO, BENGKULU DAN POTENSINYA SEBAGAI PENDEGRADASI SELULOSA [Diversity of Yeasts Isolated From Natural Resources of Enggano Island, Bengkulu and Its Cellulolytic Potency] Sumerta, I Nyoman; Kanti, Atit
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i3.2220

The present study revealed the occurrence and diversity of yeasts from natural resources of Enggano Island. The yeasts were isolated from soil, sediment, leaf, leaf litter, fruit, and flower. Isolation was performed using direct plating, membrane filtration, ballistospore-falling and enrichment culture using glucose, xylose and xylan as carbon source. After isolating, yeasts were screened for its cellulolytic potency. The occurrence of yeasts in different isolation technique are clearly found. Most of 87 yeasts isolated, abundant in leaf, soil, and sediment sample. The total yeasts isolated were classified into 32 species based on D1/D2 LSU 26S rDNA sequences analysis which genera of Candida were predominant (33%). The predominant yeast species were identified as Candida tropicalis (16,1%), Cyberlindnera saturnus (16,1%), and Rhodosporidium paludigenum (11,5%). Screening on carboxymethyl cellulose medium, there are 43 isolates within 22 yeasts species have cellulolytic activity. Four species of Basidiomycetous have high activity which includes Pseudozyma antartica Y15Eg001; Pseudozyma hubeiensis Y15Eg015; Anthracocystis chrysopogonis Y15Eg072; Cryptococcus laurentii Y15Eg017 and two from Ascomycetous are Sarocladium bactrocephalum Y15Eg226, Y15Eg227. Some of cellulolytic yeast isolates are indicated as new species candidate and required to reconfirm in another conserve regions to ensure its taxonomic position. Various yeasts isolated from Enggano Island with its cellulolytic potency should contribute to scientific information regarding microbial genetic resources of outer islands of Indonesia.

DIVERSITAS DAN PROFIL METABOLIT SEKUNDER JAMUR ENDOFIT YANG DIISOLASI DARI TUMBUHAN GAMBIR (Uncaria gambler) SERTA AKTIVITAS BIOLOGISNYA SEBAGAI ANTIBAKTERI Jamal, Yuliasri; Ilyas, Muhamad; Kanti, Atit; Agusta, Andria
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Five kinds of endophytic filamentous fungi i.e. Pestalotiopsis sp. GNC, Fusarium sp. GNC-A, Fusahum sp. GNC-B, Pestalotiopsis sp. GUC and Fusarium sp. GUC were isolated from surface sterilized of steems of gambir nasi and gambir udang (Uncaria gambier Roxb.) collected from Rengat, Riau Province,Indonesia. Identification of the endophytic fungi were done through morphologically observations and chemotaxonomically approach. In an antibacterial assays, the ethyl acetate extract of the Pestalotiopsis sp.GNC cultures showed their activity against pathogenic bacteria, Eschericia coli, Staphylococcus aureus and Bacillus sublilis.Beside the above three pathogenic bacteria, the ethyl acetate extract of Fusarium sp. GNC-B culture was also active against

Co-Authors Ahmad Thontowi ANDO KATSUHIKO ANDRIA AGUSTA Ariyanti Oetari Arwan Sugiharto CHIHARU NAKASHIMA Endang Kusdiyantini Endang Sukara, Endang Euis HERMIATI Faradila Ayu, Near Putri Habibi, Moh Heddy Julistiono HEDDY JULISTIONO Helbert Helbert I Made Sudiana I Made Sudiana I Made Sudiana latifah K Darusman LATUPAPUA, H.J.D. Latupapua, H.J.D. Maman Rahmansyah Mia Kusmiati Muhamad Ilyas Muhamad Ilyas, Muhamad Muhammad Ilyas Mujahidah, Shofia Mujahidah, Shofia N Nurhayati Nampiah Sukarno Napitupulu, Toga P. Napitupulu, Toga Pangihotan Purbani, Debora Christin Putri, Ade Lia RASTI SARASWATI Senlie Octaviana Sudiana, I. Made Sumerta, I Nyoman Sumerta, I Nyoman Sumerta, I Nyoman Sumerta, I Nyoman Suprapedi Suprapedi Wellyzar Sjamsuridzal Yantyati Widyastuti YULIASRI JAMAL Yuliasri Jamal

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