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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences
Mujizat Kawaroe
Department of Marine Science, Bogor Agricultural University, Indonesia
Earth & Planetary Sciences

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The Effect of CO2 Injection on Macroalgae Gelidium latifolium Biomass Growth Rate and Carbohydrate Content Mujizat Kawaroe; Adriani Sunuddin; Dina Augustine; Dea Fauzia Lestari
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 2 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.053 KB) | DOI: 10.14710/ik.ijms.21.2.85-92

Abstract

There are many species of macroalga grow in marine ecosystem and potentially as raw material for bioethanol resource. Bioethanol is a conversion result of carbohydrate, one of macroalgae biomass content. The exploration of macroalgae require information about  growth rate ability to determine availability in the nature. This research analyze growth rate and carbohydrate content of marine macroalga Gelidium latifolium on cultivation using varied injection of carbon dioxide and aeration. The treatments were control (K), 2000 cc CO2 injection and aeration (P1), 3000 cc CO2 injection and aeration (P2), 2000 cc CO2 injection without aeration (P3), and 3000 cc CO2 injection without aeration (P4). Samples weight were 3 gram in early cultivation on laboratorium scale for 42 days observation. The results showed that the daily growth rate Gelidium latifolium during the study ranged from 0.02-1.06%. The highest daily growth rate was 1.06±0.14% (P2). Carbohydrate yield was 18.23% in early cultivation then 19.40% (K and P2), 20.40% (P1), 16.87% (K3), and 16.40% (P4) after cultivation. The high of carbohydrates value may not guarantee the sustainable Gelidium latifolium biomass utilization as raw material for bioethanol production because of the low growth rate, thus it is necessary to modified and encourage cultivation method effectively. Keywords: CO2 injection, growth rate, carbohydrate, macroalgae, Gelidium latifolium
Potential Marine Fungi Hypocreaceae sp. as Agarase Enzyme to Hydrolyze Macroalgae Gelidium latifolium (Potensi Jamur Hypocreaceae sp. sebagai Enzim Agarase untuk menghidrolisis Makroalga Gelidium latifolium) Mujizat Kawaroe; Dwi Setyaningsih; Bertoka Fajar SP Negara; Dina Augustine
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 1 (2015): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.08 KB) | DOI: 10.14710/ik.ijms.20.1.45-51

Abstract

Agarase dapat mendegradasi agar ke oligosakarida dan memiliki banyak manfaat untuk makanan, kosmetik, dan lain-lain. Banyak spesies pendegradasi agar adalah organismelaut. Beberapa agarase telah diisolasi dari genera yang berbeda dari mikroorganisme yang ditemukan di air dan sedimen laut. Hypocreaceae sp. diisolasi dari air laut Pulau Pari, Kepulauan Seribu, Jakarta, Indonesia. Berdasarkan hasil identifikasi gen 16S rDNA dari 500 basis pasangan, isolat A10 memiliki 99% kesamaan dengan Hypocreaceae sp. Enzim agarase ekstraseluler dari Hypocreaceae sp. memiliki pH dan suhu optimum pada 8 TrisHCl (0,148 μ.mL-1) dan 50°C (0,182 μ.mL-1), masing-masing. Enzim Agarase dari Hypocreaceae sp. mencapai kondisi optimum pada aktivitas enzim tertinggi selama inkubasi dalam 24 jam (0,323 μ.mL-1). SDS page mengungkapkan bahwa ada dua band dari protein yang dihasilkan oleh agarase dari Hypocreaceae sp. yang berada di berat molekul 39 kDa dan 44 kDa dan hidrolisis Gelidium latifolium diperoleh 0,88% etanol. Kata kunci: enzim agarase, Hypocreaceae sp., hidrolisis, fungi, rDNA. Agarase can degradedagarto oligosaccharide and has a lot of benefits for food, cosmetics, and others. Many species of agar- degrader are marine-organism. Several agarases have been isolated from different genera of microorganisms found in seawater and marine sediments. Hypocreaceae sp. was isolated from sea water of Pari Islands, Seribu Islands, Jakarta, Indonesia. Based on the results of the 16S rDNA gene identification of 500 base pairs, A10 isolates had 99 % similarity toHypocreaceae sp. The extracellular agarase enzyme from Hypocreaceae sp. have optimum pH and temperature at 8 TrisHCl (0.148 µ.mL-1) and 50 °C (0.182 µ.mL-1), respectively. Agarase enzyme of Hypocreaceae sp. reach an optimum condition at the highest enzyme activity during incubation in 24 hours (0.323 µ.mL-1). SDS Page revealed that there are two bands of protein produced by agarase of Hypocreaceae sp. which are at molecular weight of 39 kDa and 44 kDa and hydrolisis of Gelidium latifolium obtained 0,88% ethanol. Key words: agarase enzym, Hypocreaceae sp., hydrolysis, marine fungi, rDNA 
Co-Authors Adriani Sunuddin Bertoka Fajar SP Negara Dea Fauzia Lestari Dina Augustine Dina Augustine Dwi Setyaningsih