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Aktivitas Antibiotik terhadap Biofilm Staphylococcus aureus Isolat Preputium Sapi Aceh Dewi, Maryulia; Darmawi, Darmawi; Nurliana, Nurliana; Karmil, T. Fadrial; Helmi, T. Zahrial; Fakhrurrazi, Fakhrurrazi; Erina, Erina; Abrar, Mahdi; AK, M. Daud; Admi, Masda
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Biofilm is one of parameters for determining pathogenic pathways of a bacterium. Bacterial virulence activities can be attenuated by antibiotics with inhibiting biofilm. The aim of this study was to examine the characteristics and activities of antibiotics against the biofilm of Stapylococcus aureus isolate which taken from Aceh cattle prepuce. The study was exploratory and experimental, using a Completely Randomized Design (RAL) factorial pattern using three types of antibiotics, three concentrations and replications. Antibiotics were tetracycline, oxytetracycline and phosphomycin with concentrations of 10%, 20% and 30% (mg/mL), respectively. Therefore, the aim of this study was to examine the characteristics and activities of antibiotics to the biofilm of Stapylococcus aureus from Aceh cattle prepuce isolate. U-bottom polystyrene 96 wells microplate and Nutrient Broth (NB) medium was used to biofilm bacterial while biofilm was stained on crystals violet 1%. Optical Density (OD) of biofilm were Î» 595 nm. Data were analyzed by using Analysis of Variance (ANOVA). The results showed that the activity of Staphylococcus aureus was moderately positive, i.e 2,667cell/Î¼L. It showed that antibiotics and concetrations have significant effect (P<0.05) to inhibit biofilm staphylococcus aureus. In additioon, tetracycline 30% (mg / mL) has the best ability to inhibit biofilms from 52,18% to 1,262 cell/Î¼L. Based on that fact, it can be concluded that the virulence of Staphylococcus aureus was moderate positive to be false positive.

Anti-bacterial activity of rosella flowers extract (Hibiscus sabdariffa linn) in inhibiting bacterial growth methicillin-resistant Staphylococcus aureus Zinatul Hayati; Winda Yulia; T. Fadrial Karmil; Abdullah Azmy
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Infections caused by bacteria Methycillin Resistant Staphylococcus aureus (MRSA) has become a worldwide health problem because of its increasing incidence. Rosella flower (Hibiscus sabdariffa Linn) has been known to have efficacy as an antibacterial. The purpose of this study is to determine the antibacterial activity of rosella flower extract in inhibiting the growth of MRSA. This study is an experimental research laboratory using rosella flower extract as a treatment, vancomycin as a positive control and distilled water as negative control. Fresh and dried rosella flowers crushed and then macerated with 96% ethanol for 24 hours. The filtrate obtained was concentrated by rotary evaporator at a temperature of 35 ˚ C-40 ˚ C and then diluted to a concentration of 12.5%, 25%, 50% and 75%. Phytochemical test is then performed. S.aureus bacteria isolated from clinical specimens in RSUDZA. Test of the antibacterial activity of rosella flower extract performed using Kirby Bauer disc diffusion method. Parameters on the disc were measured in millimeters MRSA. The data obtained were statistically analyzed and grouped into categories of Greenwood (1995). Phytochemical test results indicate the presence of rosella flower extract contains flavonoids, tannins, saponins, triterpenoids and alkaloids. Test results of the inhibition of rosella flower extract concentration of 12.5%, 25%, 50% and 75% of the growth of MRSA each inhibitory zone formed with an average diameter of 14.2 mm, 19.2 mm, 22.8 mm and 24.18 mm, whereas the positive control and negative control respectively of 19.8 mm and 5 mm. The results of data analysis showed that the ethanol extract of rosella flowers significant effect in inhibiting the growth of MRSA by the value of p 0.05. Based on the criteria of Greenwood, rosella flower extract ethanol concentration of 50% and 75% belong to the category of strong inhibitory power in inhibiting the growth of MRSA, a concentration of 25% falls into the category of being and the concentration of 12.5% falls into the category of weak. The results can be concluded that rosella flower ethanol extract can inhibit the growth of MRSA.

Evaluation of viability and nutritive value of Indigofera tinctoria as a potential animal feeding in Aceh Province, Indonesia M. Aman Yaman; M. Daud; . Zulfan; Yadi Jufri; T. Fadrial Karmil
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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A series of studies related to animal feeding technology to stimulate the egg production and egg quality with the development of a cheap source of protein types and useful has been done through the evaluation of viabilityand nutritive value of Indigofera tinctoria and in vivo treatments on quail and chicken. Research conducted since 2010 to October 2012 with a focus on increasing the ability to grow the Indigofera’s seed under soaking treatment inauxin hormone and testing the nutritional value on quail and chicken. The first study related to the viability testing is done by soaking the seed of Indigofera in auxin hormone before planting in the field. The study consisted of 4treatments and 5 replications of soaking period, namely: control, B (12 hours), C (24 hours) and D (32 hours) in the solution. The parameters measured were: viability and growth of seed and dry matter content of Indigofera leaf. Thesecond study tested the nutritive value of Indigofera in the ration of laying chickens and quail. Treatments were: 0, 2, 4 and 6% of Indigofera leaf in the ration. Parameters observed in were: egg production, fertility, hatchability andegg quality. The results showed that the viability of the Indigofera’s seed with soaking treatment significantly increased to 80% and soaking for 32 hours resulted a higher viability. The treatment of soaking in auxin hormonealso increased nutrient content of indigofera leaf especially protein. In terms of nutritional value, supplement of Indigofera leaf was very useful to improve the production and quality of eggs in both local laying chickens and quail.The addition of Indigofera leaf in the ration up to 5% also reduced the feed consumption and increasead Haugh unit of egg chicken. From these results it was concluded that the viability of Indigofera tinctoria increased by soaking inauxin hormone for 12-32 hours. These treatments also stimulated the dry matter and protein contents in Indigofera’s leaf. The supplement of Indigofera tinctoria leaf as much as 1-5% in the ration also improved the egg production and quality of local chicken and quail. It was indicated that Indigofera tinctoria contains not only protein and amino acids but also vitamin and mineral for egg quality

16. The Number Of Leukocyte And Leukocyte Differential In Broilers That Infected With Eimeria tenella And Given Neem Leaf Extract And Jaloh Extract Rizki Aulia; Sugito Sugito; M. Hasan; T. Fadrial Karmil; Gholib Gholib; Rinidar Rinidar
Jurnal Medika Veterinaria Vol 11, No 2 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

The aim of this research was to determine the effect of giving neem leaf extract combined with jaloh towards the total of leukocytes and differential leukocyte in broilers that was infected with Eimeria tenella. This research samples were 20 Cobb stain broilers 14 days old. Design the research was complete randomized design with five treatments and each treatment consists of four repetitions. First treatment (P1) as negative control which was only given mineral water; second treatment (P2) as positive control was is given 5 mg/L anti-stress commercial in drinking water; Third treatment (P3) was given 1000 mg/L Jaloh extract; Fourth treatment (P4)was given 250mg/L neem extract; and fifth treatment (P5)was given 1000mg/L jaloh extract combined with 250mg/L neem extract. Anti-stress commercial and extract treatment dissolved in drinking water. The treatment start from 08.00 until 18.00 for ten days. Next in the 11th day (chicken 25 days old)was inoculated Eimeria tanella sporulatif as much as 1 x 104 ookista/ml orally. Then the blood was taken sampling when the chicken 30 days old (five days after infected). Blood sampling was done in the brachial vein. Furthermore, the number of leukocytes and differential leukocyte.Was calculated data were analyzed using complete randomized design with the help of SPSS for Windows 1.8 program. The result of this research showed that giving jaloh and neem leaf extract were not giving significant effect (P0,05) towards the number of leukocyte as well as differential leukocyte of broilers that was infected with Eimeria tenella. The conclusion of this research were giving jaloh leaf extract and neem for 10 days was not giving significant effect towards number of leukocytes and differential leukocyte broilers that infected with Eimeria tenella

6. The Effect of Celery Leaves Infusa (Apium graveolens L) on Reducing Level of Blood Glucose on Rat (Rattus norvegicus) Induced by Alloxan Fatin Syarifahnur; Roslizawaty Roslizawaty; Amiruddin Amiruddin; Muhammad Hasan; T. Fadrial Karmil; Hamdani Budiman
Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

This study aims to investigate the effect of celery leaf infusa on decreasing level of blood glucose in rat (Rattus norvegicus). Twenty five rats were divided into 5 groups namely first treatment group (P1) as negative control. Second treatment group (P2) as positive control, rats were induced with alloxan. Third treatment group (P3) rats were induced with alloxan and given 5% celery leaf infusa. Fourth treatment group (P4) rats were induced with alloxan and given 10% celery leaf infusa. Fifth treatment group (P5) rats were induced alloxan and given 15% celery leaf infusa for 14 days. The level of blood glucose of the rat was determined before treatment, after given aloksan and after given celery leaf infusa. Data were analyzed using Analysis of Varians (ANOVA). Results showed that administration of celery leaf infusa for 14 days, show significant effect to decreasing level of glucose in rat (P0.01). It can be concluded that the administration of celery leaf infusa concentrated 5%, 10% and 15% for 14 days show significant effect to decreased level of glucose in rats.

VIABILITAS LARVA PADA NYAMUK Aedes aegypti, Aedes albopictus DAN Culex quinquefasciatus DENGAN BERBAGAI TINGKAT INFEKSI MIKROFILARIA (LARVA VIABILITY IN MOSQUITO Aedes aegypti, Aedes albopictus AND Culex quinquefasciatus WITH VARIOUS INFECTIONS OF MICROFILARIA) Auliya rahmi Ritonga; T. Fadrial Karmil; T. Zahrial Helmi; Winaruddin Winaruddin; M. Hanafiah; Razali Daud; M Daud AK
JURNAL ILMIAH MAHASISWA VETERINER Vol 5, No 1 (2020): NOVEMBER-JANUARI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v5i1.8578

Penelitian ini bertujuan untuk mengetahui viabilitas larva yang berperan aktif pada nyamuk Aedes aegypti, Aedes albopyctus dan Culex quinquefasciatus mulai dari larva satu hingga mencapai larva tiga atau larva infektif. Sampel yang digunakan dalam penelitian ini adalah anjing reservoir yang berjumlah 3 ekor dengan tingkat infeksi mikrofilaria 330 mf/ml darah, 1.430 mf/ml darah dan 10.395 mf/ml darah. Nyamuk yang diinfeksikan dengan berbagai tingkat infeksi mikrofilaremik tersebut diamati dan dilakukan pembedahan pada hari 1,3,6,9 dan 12. Hasil penelitian tersebut menunjukkan bahwa viabilitas larva pada nyamuk Ae. aegypti, Ae. albopyctus dan C. quinquefasciatus yang dilakukan penginfeksian dengan tingkat infeksi 330 mf/ml darah berkisar 80,08 %, 70,26 % dan 78,47 %; tingkat infeksi 1.430 mf/ml darah yaitu 65,72 %, 62,31 % dan 61,93 %; serta pada tingkat infeksi tinggi 10.395 mf/ml darah berkisar 53,92%, 55,79 % dan 54,27 %. Sehingga dapat disimpulkan bahwa ketiga jenis nyamuk tersebut berpotensi untuk menyebarkan penyakit dirofilariasis, hal ini sangat tergantung dari jumlah mikrofilaria yang terkandung dalam host defenitif. Semakin rendah tingkat infeksi maka semakin besar kemampuan nyamuk berpotensi menyebarkan penyakit.This study aims to determine the viability of the larvae that play an active role in Aedes aegypti mosquitoes, Aedes albopyctus and Culex quinquefasciatus from larvae one to reach the larvae of three or infective larvae. The samples used in this study were 3 reservoir dogs with microfilariae infection rate of 330 mf / ml of blood, 1,430 mf / ml of blood and 10,395 mf / ml of blood. Mosquitoes infected with various levels of microfilinemic infection were observed and performed surgery on days 1,3,6,9 and 12. The results showed that the viability of larvae in Ae mosquitoes. aegypti, Ae. albopyctus and C. quinquefasciatus infected with infection rate of 330 mf / ml of blood ranged 80.08%, 70.26% and 78.47%; infection rate 1.430 mf / ml of blood that is 65,72%, 62,31% and 61,93%; as well as at high infection rate 10,395 mf / ml of blood ranged 53.92%, 55.79% and 54.27%. So it can be concluded that the three types of mosquitoes have the potential to spread disease diropfilariasis, it is highly dependent of the number of microfilaria contained in the host defenitif. The lower the infection rate the greater the ability of mosquitoes to spread disease.

PENGARUH JUMLAH MIKROFILARIA PADA ANJING PENDERITA Canine Heartworm Diseases TERHADAP ANGKA KEMATIAN NYAMUK Aedes aegypti ISOLAT LAPANG (The Effect Of Microfilirae Amount On Dogs With Canine Heartworm Diseases On The Mortality Rate Of Aedes Aegypti Field Isolate) dzulfikar faizin romas; T. Fadrial Karmil; M Hanafiah; Winaruddin Winaruddin; M Hambal; Muttaqien Muttaqien; Nazaruddin Nazaruddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 5, No 4 (2021): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v5i4.9376

Penelitian ini bertujuan mengetahui pengaruh jumlah mikrofilaria pada anjing penderita Canine Heartworm Diseases terhadap angka kematian nyamuk Ae. aegypti. Sampel yang digunakan adalah seribu dua ratus ekor nyamuk dengan lima kali ulangan dan tiga ekor anjing dengan kategori infeksi mikrofilaremik tinggi 10.395 mf/ml, sedang 1.430 mf/ml, dan ringan 330 mf/ml serta satu anjing kontrol 0 mf/ml positif. Anjing di kandangkan dengan nyamuk, ketika nyamuk selesai menghisap kemudian dikoleksi dan diamati selama 13 hari. Data angka kematian nyamuk Ae aegypti akan dianalisis, secara statistik untuk melihat standart deviasi, dipastikan dengan uji ANOVA SPSS, Rancangan Acak Lengkap (RAL), dan disajikan dalam bentuk grafik. Hasil penelitian menunjukan angka kematian pada perlakuan nyamuk kontrol 0 mf/ml dengan rata-rata 0.68±0.94. Sedangkan angka kematian nyamuk pada perlakuan di hari pertama pengamatan rata-rata angka kematian nyamuk pada tingkat rendah 330 mf/ml sebanyak 12.00±0.75, sedang 22.00±0.75, dan tinggi 34.00±0.9, angka kematian mulai meningkat kembali di hari ketiga, pada tingkat rendah 330 mf/ml 9.87±0.87, sedang 13.85±1.24, tinggi 16.84±1.83. Angka kematian kembali meningkat pada hari ke sepuluh dengan rata-rata rendah 15.97±9.65, sedang 31.37±2.77, tinggi 40.22±5.95. Hasil ANOVA pada pengamatan hari ke 13, nilai p dari perlakuan adalah 0.000 (p 0.05), maka dari itu kita menolak H0. Kesimpulan semakin tinggi tingkat infeksi, semakin tinggi angka kematian nyamuk sehingga nyamuk Ae. aegypti dengan tingkat infeksi yang rendah bisa menjadi potensial vektor.(This study aimed to know the effect of the amount of microfilariae on dogs with Canine Heartworm Diseases on the mortality rate of mosquitoes Aedes Aegypti. The sample used was one thousand two hundred mosquitoes with five repeatations and three dogs with a high category of microfilaremic infection 10,395 mf / ml, moderate 1,430 mf / ml, and low 330 mf / ml and one control dog 0 mf / ml positive. The dog was caged up with mosquitoes, when the mosquitoes had finished sucking then it was collected and observed for thirteen days. The mortality rate of Ae aegypti mosquito was analyzed, statistically looked at the standard deviation, confirmed by the ANOVA SPSS Complete Random Design test, and presented in graphical form. The results showed that the mortality rate in controlled mosquito treatment 0 mf / ml was 0.68±0.94. While the mortality rate of mosquitoes in the treatment on the first day of observing the average mosquitoes mortality rate at the low level of 330 mf / ml was as much as 12.00 ± 0.75, moderate 22.00 ± 0.75, and a high of 34.00 ± 0.9, the mortality rate began increasing again on the third day, at the low level 330 mf / ml 9.87 ± 0.87, medium 13.85 ± 1.24, high 16.84 ± 1.83. The death rate again increased on the tenth day with the low average of 15.97 ± 9.65, moderate 31.37 ± 2.77, high 40.22 ± 5.95. Based on ANOVA results, the p value of the treatment was 0.000 (0.05), therefore we rejected H0. The higher the infection rate, the higher the mosquitoes mortality rate so that the mosquiotoes Ae Aegypti with the low infection rate with the fewest average number of mortality rate can be a potential vector).

Deteksi Sistiserkus Cacing Pita (Taenia spp) pada babi (Sus scrofa) di Rumah Potong Hewan Medan Sumatera Utara Connie Asty Pakpahan; Muttaqien Bakri; M. Hanafiah; Yudha Fahrimal; Nuzul Asmilia; T. Fadrial Karmil
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 1 (2021): NOVEMBER-JANUARI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i1.5213

Penelitian ini bertujuan mendeteksi ada atau tidaknya sistiserkus cacing pita (Taenia spp) pada babi yang dipotong di rumah potong hewan Medan Sumatera Utara. Sistiserkus merupakan metacestoda dari larva Taenia solium. Deteksi sistiserkus sangat diperlukan untuk memahami pola distribusi, prevalensi dan cara penularan penyakit (siklus hidup T. solium). Penelitian ini dilaksanakan pada Maret 2017. Sampel yang digunakan sebanyak 20 ekor dengan menggunakan dengan simple random sampling. Sampel yang sudah dikumpulkan diperiksa secara makroskopik postmortem dengan cara melihat tanda- tanda sistiserkosis pada daging seperti lepuhan pada sampel. Data yang di peroleh akan di analisis secara deskriptif. Hasil pemeriksaan menunjukkan seluruh sampel negatif terdeteksi sistiserkus asal babi-babi milik peternak yang di potong di RPH.

PENGARUH PAPARAN TIMBAL (Pb) TERHADAP LAJU PERTUMBUHAN IKAN NILA (Oreochromis nilloticus) Nur Inda Rahayu; Rosmaidar Rosmaidar; M. Hanafiah; T. Fadrial Karmil; T. Zahrial Helmi; Razali Daud
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 4 (2017): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Penelitian ini bertujuan mengetahui tingkat konsentrasi timbal (Pb) yang berpengaruh terhadap laju pertumbuhan ikan nila (Oreochromis nilloticus). Penelitian ini menggunakan ikan nila sebanyak 40 ekor dengan kriteria: sehat; bobot badan 15 – 18 gram; umur ± 2 bulan; jenis kelamin jantan. Penelitian ini menggunakan 4 kelompok perlakuan, P0 sebagai kontrol ikan hanya diberi pakan pelet, P1 diberikan paparan timbal 6,26 mg/L dan pakan pelet, P2 diberikan paparan timbal 12,53 mg/L dan pakan pelet dan P3 diberikan paparan timbal 25,06 mg/L dan pakan pelet, masing-masing perlakuan terdiri dari 10 ekor ikan nila. Perlakuan dilakukan selama 30 hari dan Pengukuran pertumbuhan ikan dilakukan setiap 10 hari sekali dengan cara menimbang bobot dan mengukur panjang tubuh setiap individu ikan. Hasil penelitian menunjukkan rata-rata panjang tubuh ikan nila P0 (9,45±1,06), P1 (8,89±0,90), P2 (8,86±0,87), dan P3 (8,66±0,85). Rata-rata berat ikan nila P0 (23,38±4,50), P1 (19,75±2,27), P2 (19,15±2,10), dan P3 (18,65±2,00). Laju pertumbuhan spesifik P0 (38,7%), P1 (8,3%), P2 (4,3%), dan P3 (3,3%). Laju pertumbuhan panjang harian individu ikan P0 (0,46 mm/d), P1 (0,27 mm/d), P2 (0,21 mm/d), dan P3 (0,19 mm/d). Kesimpulan dari penelitian bahwa konsentrasi timbal dan lamanya paparan berpengaruh terhadap laju pertumbuhan ikan. Konsentrasi timbal yang sangat berpengaruh adalah 25,06 mg/L. ABSTRACTThe aims of this research was to find out to determine the level of Lead (Pb) concentration that affect the rate of growth tilapia (Oreochromis nilloticus). This study used 40 tilapia with criteria: healthy; body weight 15-18 gram; age ± 2 month; male sex. This study used 4 treatment groups, P0 as control, fish fed only pellets, P1 was given lead exposure 6,26 mg/L and pellet, P2 was given lead exposure 12,53 mg/L and pellet and P3 was given lead exposure 25,06 mg/L and pellet. Each treatment consisted of 10 tilapia fish. Treatment carried out for 30 days and measure every 10 days. The results showed the average length of tilapia fish P0 (9,45±1,06b); P1 (8,89±0,90a); P2 (8,86±0,87a) and P3 (8,66±0,85). Average weight of tilapia fish P0 (23,38±4,50); P1 (19,75±2,27); P2 (19,15±2,10) and P3 (18,65±2,00). Spesific growth rate P0 (38,7%); P1 (8,3%); P2 (4,3%) and P3 (3,3%). The rate of long-term growth of individual fish P0 (0,46 mm/d); P1 (0,27 mm/d); P2 (0,21 mm/d) and P3 (0,19 mm/d). In conclusion, it showed that the concentration of lead (Pb) and the duration of exposure has affect the growth rate of fish. The influential concentration of lead is 25,06 mg/L.

PENGARUH JUMLAH MIKROFILARIA PADA ANJING PENDERITA Dirofilaria immitis TERHADAP ANGKA KEMATIAN NYAMUK Culex quinquefasciatus ISOLAT LAPANG (Effect Of Microfilirae On Dogs With Dirofilaria immitis On the Mortality Rate Of Culex quinquefasciatus With Isolate) Afifah Nur Oriyasmi; T. Fadrial Karmil; Winaruddin Winaruddin; Farida Athaillah; Abdullah Hamzah; Ummu Balqis; M Daud AK
JURNAL ILMIAH MAHASISWA VETERINER Vol 4, No 3 (2020): MEI-JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v4i3.8576

Penelitian ini bertujuan untuk mengetahui pengaruh jumlah mikrofilaria pada anjing penderita D. immitis terhadap angka kematian nyamuk Cx. quinquefaciatus isolat lapang. Sampel yang digunakan adalah empat ekor anjing, tiga ekor anjing yang terinfeksi D. immitis dengan jumlah mikrofilaria tingkat infeksi 330 mf/ml darah, 1.430 mf/ml darah, dan 10.395 mf/ml darah serta satu anjing sebagai control (negatif D. immitis). Nyamuk Cx. quinquefasciatus yang diinfeksikan dengan berbagai tingkat infeksi mikrofilaria tersebut diamati angka kematian nyamuk selama 13 hari. Hasil penelitian menujukan bahwa angka kematian nyamuk Cx. quinquefasciatus dengan tiga tingkatan infeksi terjadi kematian yang tinggi pada hari ke-10 infeksi berat 31,56 %, sedang 21,7 % dan ringan 15.4 % hal ini disebabkan oleh pergerakan dan aktivitas biologis larva yang dapat merusak tubulus malpighia. Sehingga dapat disimpulkan Angka kematian nyamuk Cx. quinquefasciatus dengan jumlah mikrofilaria tinggi (10.395 mf/ ml darah ) adalah 13,1 %, tingkat infeksi sedang ( 1.430 mf/ml darah ) adalah 10,4 % dan tingkat rendah ( 330 mf/ml darah ) adalah 9,1 % serta kontrol 0,2 %. Sehingga semakin tinggi tingkat infeksi mikrofilaria maka semakin tinggi angka kematian nyamuk.This study aims to determine the effect of the number of microfilariae in dogs with D. immitis to mosquito mortality. Cx quinquefaciatus isolate field. The samples used were four dogs, three dogs infected with D. immitis with microfilaria number of infection rate 330 mf / ml blood, 1,430 mf / ml blood, and 10,395 mf / ml of blood and one dog as control (negative D. immitis) . Mosquito Cx. quinquefasciatus infected with various levels of microfilaria infection was observed mortality rate for 13 days. The results showed that the mortality rate Cx. quinquefasciatus with three levels of infection occurs high mortality on day 10 this is caused by the movement and biological activity of larvae that can damage the tubule malpighia. So it can be concluded mosquito mortality rate. Cx quinquefasciatus with high microfilariae (10.395 mf / ml blood) was 13.1%, moderate infection rate (1.430 mf / ml blood) was 10.4% and low level (330 mf / ml blood) was 9.1% and control 0.2%. So the higher the rate of microfilaria infection, the higher the mortality rate of mosquitoe.

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