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Exploration, Isolation and Quantification of β-carotene from Bacterial Symbion of Acropora sp. NAELY K. WUSQY; LEENAWATY LIMANTARA; FERRY F KARWUR
Microbiology Indonesia Vol. 8 No. 2 (2014): June 2014
Publisher : Indonesian Society for microbiology

In the microbial world, pigments are one of the most conspicuous traits. Marine bacteria associated with Acropora sp. collected from Taka Cemara, Karimunjawa Islands were screened for the production of a yellow pigment. The isolation of bacterial symbionts from Acropora sp. on Zobell 2216E medium resulted in one bacterium, KJ5, positively synthesized carotenoids. By reverse phase HPLC analysis, one peak of the pigment types was identified as a β-carotene peak which appeared at 60.24 min. Then, sample of the β-carotene was collected and identified according to their spectral characteristics and compared with the published data in different types of solvent. Based on the HPLC analysis, the total β-carotene contents were calculated by converting the broad absorption of β-carotene. Molecular identification of the bacterium KJ5 using 16S rDNA showed that bacterium KJ5 was closely related to Erythrobacter flavus with 96% homology value.

Pitarah Manusia Nusa Tenggara Timur Berdasarkan Ceritera Kromosom Y Meti OFI Tefu; Ferry Fredy Karwur
Scientiae Educatia: Jurnal Pendidikan Sains Vol 6, No 2 (2017): December (2017)
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

STUDY OF HUMAN ANCESTORS NUSA TENGGARA TIMUR INDONESIA BASED ON Y CHROMOSOME.Genetic studies conducted in NTT region focused on four islands namely Flores, Sumba, Alor, and Timor. The islands of NTT are a transition zone between Papuan and Austronesian speakers. Genetically there is also an intensive mixing. The dominant genetic of tribes in Alor island is Papua / Melanesia, but on the other islands there is almost uniform mixing. In the western part of Sumba Island there is a majority area of the Papuan/ Melanesian genetic type, while the tribes in eastern Sumba, Sabu and Rote are Austronesian. Genetic mixing is also seen on Timor Island. West Timor is the more dominantly Austronesian type, while central and eastern Timor are a mixture of Papua/ Melanesia with Austronesian. On such Flores islands, the tribes in the eastern and central regions have the Papua/ Melanesian type, while the western part is almost entirely of Austronesian type. The tribes on Solor, Lembata and Adonara Islands also have mixed types between Austronesia and Papua/ Melanesia. Based on paternal lineage, the blood flowing in NTT people consists of seven line ages of East Asia, Taiwan, Southeast Asia, Arab, Japan, Europe and Papua/ Melanesia, characterized by the detection of thirty-two Y-DNA markers: C-M130/ RPS4Y, F-P14/F-M89 (East Asia), NO-M214, O-M175, O-M119, O-M95, O-P203, O-M110, O-M122, O-M134, O-P201, O-JST3002611 (Taiwan), K-M526, K-P397, K-P405, K-P79, K-P336, P-P295, Q-M242, Q-P36 (Southeast Asia), J-M172 (Arab), C-P343, D-M116 (Japan), E-P1 (Europe),C-M38, C-M208, C-P355, M-P256, M-M4, M-P34, S-M230, S-M254, S-P377 (Melanesia).Studi genetik yang dilakukan di daerah NTT difokuskan pada empat pulau yakni Flores, Sumba, Alor, dan Timor. Pulau-pulau di NTT menjadi zona transisi antara penutur Papua dan Austronesia. Secara genetika juga terjadi pembauran yang intensif. Suku di pulau Alor, genetiknya dominan Papua/ Melanesia, namun di pulau-pulau lain terjadi pembauran hampir merata. Di Pulau Sumba bagian barat ada kawasan yang genetikanya mayoritas bertipe Papua/ Melanesia, sedangkan suku di Sumba bagian timur, Sabu dan Rote bertipe Austronesia. Pembauran genetika juga terlihat di Pulau Timor. Timor barat lebih dominan bertipe Austronesia sedangkan Timor bagian tengah dan timur merupakan campuran Papua/ Melanesia dengan Austronesia. Di pulau florespun demikian, yakni suku-suku di bagian timur dan tengah memiliki tipe Papua/ Melanesia, sedangkan bagian barat hampir seluruhnya memiliki tipe Austronesia. Suku-suku di pulau Solor, Lembata dan Adonara juga memiliki tipe campuran antara Austronesia dan Papua/ Melanesia. Berdasarkan garis keturunan ayah, darah yang mengalir dalam orang NTT terdiri atas tujuh garis keturunan yaitu Asia Timur, Taiwan, Asia Tenggara, Arab, Jepang, Eropa dan Papua/ Melanesia, yang ditandai dengan terdeteksinya tiga puluh tiga penanda Y-DNA yakni: C-M130/ RPS4Y, F-P14/F-M89 (Asia Timur), NO-M214, O-M175, O-M119, O-M95, O-P203, O-M110, O-M122, O-M134, O-P201, O-JST3002611 (Taiwan), K-M526, K-P397, K-P405, K-P79, K-P336, P-P295, Q-M242, Q-P36 (Asa Tenggara), J-M172 (Arab), C-P343, D-M116 (Jepang), E-P1 (Eropa),C-M38, C-M208, C-P355, M-P256, M-M4, M-P34, S-M230, S-M254, S-P377. (Melanesia)

Pitarah Manusia Nusa Tenggara Timur Berdasarkan Ceritera Kromosom Y Meti OFI Tefu; Ferry Fredy Karwur
Scientiae Educatia: Jurnal Pendidikan Sains Vol 6, No 2 (2017): December (2017)
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

STUDY OF HUMAN ANCESTORS NUSA TENGGARA TIMUR INDONESIA BASED ON Y CHROMOSOME.Genetic studies conducted in NTT region focused on four islands namely Flores, Sumba, Alor, and Timor. The islands of NTT are a transition zone between Papuan and Austronesian speakers. Genetically there is also an intensive mixing. The dominant genetic of tribes in Alor island is Papua / Melanesia, but on the other islands there is almost uniform mixing. In the western part of Sumba Island there is a majority area of the Papuan/ Melanesian genetic type, while the tribes in eastern Sumba, Sabu and Rote are Austronesian. Genetic mixing is also seen on Timor Island. West Timor is the more dominantly Austronesian type, while central and eastern Timor are a mixture of Papua/ Melanesia with Austronesian. On such Flores islands, the tribes in the eastern and central regions have the Papua/ Melanesian type, while the western part is almost entirely of Austronesian type. The tribes on Solor, Lembata and Adonara Islands also have mixed types between Austronesia and Papua/ Melanesia. Based on paternal lineage, the blood flowing in NTT people consists of seven line ages of East Asia, Taiwan, Southeast Asia, Arab, Japan, Europe and Papua/ Melanesia, characterized by the detection of thirty-two Y-DNA markers: C-M130/ RPS4Y, F-P14/F-M89 (East Asia), NO-M214, O-M175, O-M119, O-M95, O-P203, O-M110, O-M122, O-M134, O-P201, O-JST3002611 (Taiwan), K-M526, K-P397, K-P405, K-P79, K-P336, P-P295, Q-M242, Q-P36 (Southeast Asia), J-M172 (Arab), C-P343, D-M116 (Japan), E-P1 (Europe),C-M38, C-M208, C-P355, M-P256, M-M4, M-P34, S-M230, S-M254, S-P377 (Melanesia).Studi genetik yang dilakukan di daerah NTT difokuskan pada empat pulau yakni Flores, Sumba, Alor, dan Timor. Pulau-pulau di NTT menjadi zona transisi antara penutur Papua dan Austronesia. Secara genetika juga terjadi pembauran yang intensif. Suku di pulau Alor, genetiknya dominan Papua/ Melanesia, namun di pulau-pulau lain terjadi pembauran hampir merata. Di Pulau Sumba bagian barat ada kawasan yang genetikanya mayoritas bertipe Papua/ Melanesia, sedangkan suku di Sumba bagian timur, Sabu dan Rote bertipe Austronesia. Pembauran genetika juga terlihat di Pulau Timor. Timor barat lebih dominan bertipe Austronesia sedangkan Timor bagian tengah dan timur merupakan campuran Papua/ Melanesia dengan Austronesia. Di pulau florespun demikian, yakni suku-suku di bagian timur dan tengah memiliki tipe Papua/ Melanesia, sedangkan bagian barat hampir seluruhnya memiliki tipe Austronesia. Suku-suku di pulau Solor, Lembata dan Adonara juga memiliki tipe campuran antara Austronesia dan Papua/ Melanesia. Berdasarkan garis keturunan ayah, darah yang mengalir dalam orang NTT terdiri atas tujuh garis keturunan yaitu Asia Timur, Taiwan, Asia Tenggara, Arab, Jepang, Eropa dan Papua/ Melanesia, yang ditandai dengan terdeteksinya tiga puluh tiga penanda Y-DNA yakni: C-M130/ RPS4Y, F-P14/F-M89 (Asia Timur), NO-M214, O-M175, O-M119, O-M95, O-P203, O-M110, O-M122, O-M134, O-P201, O-JST3002611 (Taiwan), K-M526, K-P397, K-P405, K-P79, K-P336, P-P295, Q-M242, Q-P36 (Asa Tenggara), J-M172 (Arab), C-P343, D-M116 (Jepang), E-P1 (Eropa),C-M38, C-M208, C-P355, M-P256, M-M4, M-P34, S-M230, S-M254, S-P377. (Melanesia)

Efek Beta Karoten dan Agregasi Klorofil Pada Fotostabilitas Klorofil a dalam Pelarut Aseton Costa, Junet. F. da; Karwur, Ferry F; Limantara, Leenawaty
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Chlorophyll as photosynthetic pigment has many benefits to human such as antioxidant, antibacterial, color additive,immunity, and photosensitizer. Concerning to these applications, chlorophyll a will react with oxygen and light andreduce its effectiveness. It is then important to understand the stability of chlorophyll a on oxygen and light.Stability of chlorophyll a in the presence of beta-carotene (1:1) in acetone was studied for various waterconcentrations. Each solution was exposed to red light (ë e” 630 nm) for 0.5; 1.0; 2.5; 5.0; 10; 20; 30; 40; 50; and 60minutes and their spectrum were analysed. When water was added (8.33; 16.67; 25; 33.33; 41.67; 50; 58.33; 66.67;75; and 83.33 percent) to the acetone solution of chlorophyll a, the chlorophylls aggregate, and in the presence ofbeta-carotene, the chlorophyll more stable. The water shifts the chlorophyll a spectrum toward red duringillumination with or without the presence of carotene. The formation of oligomeric chlorophyll a aggregate occurswithin 10-20 minutes after the water was applied. This strategy of aggregating the chlorophylls to dissipateexcess energy captured from light does not support the photostability of chlorophyll as much as beta-carotene.Surprisingly, when beta carotene was mixed with 66.7% and 75.0% of water, the chlorophyll a degraded 5.56% and9.71% respectively. In conclusion, the aggregate form of chlorophyll a and the presence of beta-carotene increasephotostability of chlorophyll a in acetone solution.

Analisis Komposisi dan Kandungan Karotenoid Total dan Vitamin A Fraksi Cair dan Padat Minyak Sawit Kasar (CPO) Menggunakan KCKT Detektor PDA Syahputra, M. Rio; Karwur, Ferry F; Limantara, Leenawaty
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

This study was carried out on two phases of Crude Palm Oil (CPO) to determine the total and composition ofcarotenoid and vitamin A content. Total of carotenoid was analyzed using spectrophotometer UV-Vis, and then theresult was calculated by Gross (1991) equation. The vitamin A content was calculated by NAS-NRC equation (1974).The type and composition of both phases of CPO were determined by Choo’s method (1994) by using HPLC withPhoto Diode Array (PDA) detector. The sample was prepared in two methods, with and without saponification. Theresult shows that total carotenoids in liquid and solid phase of CPO are 536 ± 13.2 g/g (liquid), 352 ± 17.7 μg/g(solid) and the vitamin A were 89.4 ± 2.2 RE (liquid), 58.7 ± 3.0 RE (solid), respectively. The carotenoid compositionsof both phases of CPO were dominated by - and -carotenes. The result shows that - and -carotenes preparedby saponification method in liquid phase are 29.03% and 60.88%, and without saponification (direct method) are28.14% and 59.44%. The result for solid phase shows that - and -carotenes by saponification are 25.89% and60.81%, and without saponification (direct method) are 30.00% and 56.92%. The research also shows the advantagesof using HPLC with PDA detector for identification and analysis of type and carotenoid composition.

Effect of Vitamin E Tocotrienol and Its Combination with Ascorbic Acid to Types of Leukocytes White Rat (Rattus norvegicus L.) Lestariningrum, Nur Auliani; Karwur, Ferry Fredy; Martosupono, Martanto
Sains Medika : Jurnal Kedokteran dan Kesehatan Vol 4, No 1 (2012): Januari-Juni 2012
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Background: Immunity is not separated from the role of leukocytes with various types and functions. Tocotrienol is one of the vitamin E group of compounds with antioxidant properties which to play an important role in cell repair. The aims to determine how much of the effect of vitamin E tocotrienols and its combination with ascorbic acid on the types of leukocytes.Design and Method: Preparation of blood lear dosage edge is a technique used to determine the effect of treatment between types of leukocytes. The design of experiment was conducted with three treatment that feed intake vitamin E tocotrienols and ascorbic acid and their combination with the control and 3 repetitions. Quantitative data leukocyte counts obtained by calculating the percentage of each type of leukocytes in each treatment group and the mean of the data obtained compared with normal leukocyte counts. In addition, the data is processed by the leukocyte counts of variance test using a completely randomized design (CRD) and BNT level of 5% to determine the differences between treatments.Result: The administration of vitamin E tocotrienols and its combination with ascorbic acid influenced significantly (P> 0.05) to increase the neutrophil are 31.66% and 33.66%, 24.33% and 22.33% respectively, when compared with controls. On the other hand, there is a decrease in the percentage of lymphocytes are 26.66% and 29.16%, 19.50% and 17.00% respectively, when compared with controls. Vitamin E tocotrienols and combined with ascorbic acid did not give significant effect (P <0.05) the percentage of eosinophils, basophils and monocytes.Conclusion: The administration of vitamin E tocotrienols and its combination with ascorbic acid to give effect to the increase in neutrophils (Sains Medika, 4(1):46-56).

Perkembangan Sensitizer pada Terapi Fotodinamika Tumor dan Kanker Hingga Penuntunan Nanopartikel (Nanoparticulate Targeting) Dengan Antibodi Monoklonal INDRAWATI, RENNY; KARWUR, FERRY F; LIMANTARA, LEENAWATY
Indonesian Journal of Cancer Vol 4, No 3 (2010): Jul - Sep 2010
Publisher : "Dharmais" Cancer Center Hospital

Show Abstract | Download Original | Original Source | Check in Google Scholar

World Health Organization (WHO) estimates both the number of patients and mortality rates due to cancer will continue to rise. Various researches were conducted in order to prevent and handle the cases of tumors and cancers, including the application of photosynthetic pigment molecules known as photodynamic therapy (PDT). Chlorophylls, the main pigment in photosynthesis, have an ability to capture light energy and control series of photobiology and photochemical processes1. In PDT, chlorophyll or its derivatives compounds act as the sensitizer which have energy excitation by light radiation (visible or near infra red), and generate some reactive oxygen species which triggers the death of cancer cells selectively (through apoptosis and / or necrosis pathway)2. Sensitizer compounds have been progressing from the first to third generation. The development of the third generation sensitizer was influenced by the advances of nanotechnology which lead to the improvement of PDT efficacy. The structure and size of nanoparticles can increase light absorption, and make the sensitizer accumulate in cancer tissues more specifically 3-4. Furthermore, nanoparticulatte targeting also interested to be studied because by conjugate functional groups, i.e. monoclonal antibody, on the sensitizer, it can improve the selectivity of therapy in targeting tumor and cancer tissues.

KOMPARASI MORFOLOGI BEBERAPA KOLONI JAMUR AKAR PUTIH (Rigidoporus microporus) DARI PERKEBUNAN KARET DI JAWA TENGAH DAN SUMATERA SELATAN Rondonuwu, Ferdy S.; Berlian, Intan; Setyawan, Budi; Karwur, Ferry F.; Langkuin, Jerry F.; Im Toy, Binerd Anthon; Martosupono, Martanto; da Costa, Junet F.
Jurnal Penelitian Karet JPK : Volume 36, Nomor 2, Tahun 2018
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v36i2.562

Rigidoporus microporus adalah jamur yang menyebabkan penyakit jamur akar putih (JAP) pada tanaman karet. Tingkat keparahan penyakit yang ditimbulkan oleh JAP berbeda antar wilayah. Penelitian ini bertujuan untuk mengetahui komparasi morfologi beberapa koloni jamur akar putih dari perkebunan karet di Jawa Tengah dan Sumatera Selatan. Sampel dikoleksi langsung dari perkebunan Merbuh dan Blimbing di Jawa Tengah dan Balai Penelitian Karet Sembawa di Sumatera Selatan. Penelitian dilakukan pada bulan November 2016-April 2017 di Laboratorium Carotenoid Antioxidant Research Center (CARC) Universitas Kristen Satya Wacana, Salatiga. Hasil yang diperoleh menunjukkan bahwa kecepatan pertumbuhan ke-17 isolat berdasar penambahan diameter koloni hingga mencapai tepi petri bervariasi yaitu 4 hari (MB8), 6 hari (MM6), 7 hari (MK2, MK3, SS1), 8 hari (MK1, MM5, MM7, SS2, SS3), 9 hari (BW1, SS5), 10 hari (MK4, BW4, SS4), 11 hari (BW2), dan 13 hari (BB3). Morfologi koloni dan hifa JAP baik dari Jawa Tengah maupun Sumatera Selatan hampir sama, yakni koloni berbentuk bulat, filamentous, elevasi ada yang rata (flat) dan ada yang sedikit timbul (raised), serta berwarna putih hingga putih gading. Hifa JAP memiliki septa, hialin, bercabang, dan tidak ada clamp connection.

KOMPARASI MORFOLOGI BEBERAPA KOLONI JAMUR AKAR PUTIH (Rigidoporus microporus) DARI PERKEBUNAN KARET DI JAWA TENGAH DAN SUMATERA SELATAN Rondonuwu, Ferdy S.; Berlian, Intan; Setyawan, Budi; Karwur, Ferry F.; Langkuin, Jerry F.; Im Toy, Binerd Anthon; Martosupono, Martanto; da Costa, Junet F.
Jurnal Penelitian Karet JPK : Volume 36, Nomor 2, Tahun 2018
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v36i2.562

Rigidoporus microporus adalah jamur yang menyebabkan penyakit jamur akar putih (JAP) pada tanaman karet. Tingkat keparahan penyakit yang ditimbulkan oleh JAP berbeda antar wilayah. Penelitian ini bertujuan untuk mengetahui komparasi morfologi beberapa koloni jamur akar putih dari perkebunan karet di Jawa Tengah dan Sumatera Selatan. Sampel dikoleksi langsung dari perkebunan Merbuh dan Blimbing di Jawa Tengah dan Balai Penelitian Karet Sembawa di Sumatera Selatan. Penelitian dilakukan pada bulan November 2016-April 2017 di Laboratorium Carotenoid Antioxidant Research Center (CARC) Universitas Kristen Satya Wacana, Salatiga. Hasil yang diperoleh menunjukkan bahwa kecepatan pertumbuhan ke-17 isolat berdasar penambahan diameter koloni hingga mencapai tepi petri bervariasi yaitu 4 hari (MB8), 6 hari (MM6), 7 hari (MK2, MK3, SS1), 8 hari (MK1, MM5, MM7, SS2, SS3), 9 hari (BW1, SS5), 10 hari (MK4, BW4, SS4), 11 hari (BW2), dan 13 hari (BB3). Morfologi koloni dan hifa JAP baik dari Jawa Tengah maupun Sumatera Selatan hampir sama, yakni koloni berbentuk bulat, filamentous, elevasi ada yang rata (flat) dan ada yang sedikit timbul (raised), serta berwarna putih hingga putih gading. Hifa JAP memiliki septa, hialin, bercabang, dan tidak ada clamp connection.

KOMPARASI MORFOLOGI BEBERAPA KOLONI JAMUR AKAR PUTIH (Rigidoporus microporus) DARI PERKEBUNAN KARET DI JAWA TENGAH DAN SUMATERA SELATAN Rondonuwu, Ferdy S.; Berlian, Intan; Setyawan, Budi; Karwur, Ferry F.; Langkuin, Jerry F.; Im Toy, Binerd Anthon; Martosupono, Martanto; da Costa, Junet F.
Jurnal Penelitian Karet JPK : Volume 36, Nomor 2, Tahun 2018
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v36i2.562

Rigidoporus microporus adalah jamur yang menyebabkan penyakit jamur akar putih (JAP) pada tanaman karet. Tingkat keparahan penyakit yang ditimbulkan oleh JAP berbeda antar wilayah. Penelitian ini bertujuan untuk mengetahui komparasi morfologi beberapa koloni jamur akar putih dari perkebunan karet di Jawa Tengah dan Sumatera Selatan. Sampel dikoleksi langsung dari perkebunan Merbuh dan Blimbing di Jawa Tengah dan Balai Penelitian Karet Sembawa di Sumatera Selatan. Penelitian dilakukan pada bulan November 2016-April 2017 di Laboratorium Carotenoid Antioxidant Research Center (CARC) Universitas Kristen Satya Wacana, Salatiga. Hasil yang diperoleh menunjukkan bahwa kecepatan pertumbuhan ke-17 isolat berdasar penambahan diameter koloni hingga mencapai tepi petri bervariasi yaitu 4 hari (MB8), 6 hari (MM6), 7 hari (MK2, MK3, SS1), 8 hari (MK1, MM5, MM7, SS2, SS3), 9 hari (BW1, SS5), 10 hari (MK4, BW4, SS4), 11 hari (BW2), dan 13 hari (BB3). Morfologi koloni dan hifa JAP baik dari Jawa Tengah maupun Sumatera Selatan hampir sama, yakni koloni berbentuk bulat, filamentous, elevasi ada yang rata (flat) dan ada yang sedikit timbul (raised), serta berwarna putih hingga putih gading. Hifa JAP memiliki septa, hialin, bercabang, dan tidak ada clamp connection.

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