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Karakteristik Morfologi dan Genetik Tanaman Penghasil Gaharu (Aquilaria spp) Endemik Sumatera Barat Satria, Benni; Gustian, Gustian; Swasti, Etti; Kasim, Musliar; Darnetti, Darnetti
Sainstek Vol 11, No 1 (2008): Vol. XI No. 1, September 2008
Publisher : Lembaga Penelitian Universitas Negeri Padang

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Abstract

Agarwood (Aquilaria spp) is plant of tha important tropical forest trees,which produces a high economically valuable fragrant resinous wood. The objective of this  study was to characterize  the agarwood based on  morphologist are  genetic distance using RAPD markers. Characterizeof the agarwood based on morphologist and genetic distance by using RAPD markers is on alternative method to see variance morphologist,genetic and condition population plant agarwood (Aquilaria sp) endemic West Sumatra. This experiment was conducted at rain forest West Sumatraand  Laboratory Agronomy Agriculture faculty Andalas University, and Laboratory Biotecnology Seameo Biotrop Bogor, during mar 2006 toDecember 2007.The result showed two group mayor cluster to agarwood i.e. Aquilaria malacensis  and Aquilaria microcarpa endemic West Sumatra by using fenotipe variabilities and RAPD markers. Broad genetic variabilties were found for long and wide leaf width. Keyword : morfologist, genetic, Agarwood, and RAPD.
BIODIVERSITY OF ARBUSCULAR MYCHORRIZAL FUNGI (AMF) ON POTATOS RHIZOSPHERE AND IT POTENTIAL AS BIOFERTILIZER Yelianti, Upik; kasli, Kasli; Kasim, Musliar; Husin, Eti Farda
Sainstek Vol 12, No 1 (2009): Vol. XII No. 1, September 2009
Publisher : Lembaga Penelitian Universitas Negeri Padang

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Abstract

AMF as the biofertilizer on several crops  has been reported, but not so much the information about biodiversity of AMF on potatos rhizosphere and its potential to colonize that crop. The research about the biodiversity of AMF has been done in laboratorium  Biology in Faculty of Agriculture of Andalas University and in laboratorium Biotecnology of Agriculture Faculty of Jambi University from November 2004 till April 2005. The sample of soil have been collected from  rhizosphire of potatos plant in Alahan Panjang, West Sumatera, and then the AMF spore are isolated and identification based on morphology and size of spores. Inoculation of single and multi spores to potatos root to see the structure of colonization and percentage of colonization. The result of identification of AMF spores show that there are many kind of spores on rhizosphere of potatos plant and the spores are dominated by: Glomus, Acaulospora, Scutellospora, Gigaspora, and Enterophospora. Unfortunately, the inoculation of single spore has no good effect to the structure of colonization and the potatos plant not vigorous and easy to be severe of stem desease. But, inoculation with multi spores show that the tipe of colonization on potatos root have the coil hyphal and intracellular vesicular. The same result also indicated that colonization with active propagule (mycelium, spores, and infected root) have the coil hyphal and intracellular vesicular. The species of AMF that have the typical characteristic is assumed as Gigaspora sp.. Key words :    AMF, potatos rhizosphere, biofertilizer
Comparing the Genetic Parameters of Three Rice Varieties on Suboptimal Land Using the SRI Method Rozen, Nalwida; Kasim, Musliar; Dwipa, Indra; Hervani, Dini
Journal of Tropical Crop Science Vol 9 No 01 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.01.47-51

Abstract

The conversion of fertile land into industrial areas and construction development has contributed to the decline of rice production in Indonesia. Therefore, it is necessary to expand the rice fields to the sub-optimal land, i.e. lands with high levels of Fe and Al and low soil pH. One of the solutions that can potentially solve the problem is choosing varieties of rice that can adapt to the suboptimal land environment; the rice varieties can be obtained by estimating rice genetic parameters and selecting the optimal planting methods. One method that can potentially improve rice yield is “System of Rice Intensification”, or SRI method. Our study was conducted in the rice fields on suboptimal land at Pasar Ambacang, Kuranji District, Padang, Sumatra, from December 2019 to April 2020. The aims of the experiment are to determine the genetic parameters and responses of the three rice varieties to suboptimal land using the SRI method. A complete randomized design with four replications was used to set up the experiment using three varieties of rice, i.e. “Batang Piaman”, “Bujang Marantau”, and “IPB3S”. Based on the plant growth measurement and analysis of the genetic parameters, we found that the plant growth characters, particularly height and panicles length, had high heritability and genetic progression values.
Pengaruh Optimalisasi Bibit dan Pupuk Kandang Ayam Terhadap Pertumbuhan Vegetatif Tanaman Padi Intensifikasi SRI Masdar Masdar; Musliar Kasim
Jurnal Agrista Vol 13, No 1 (2009): Volume 13 Nomor 1 April 2009
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Abstract

Influences of Seedling Optimizations and cock secretion fertilizer on rice plant vegetative growth in the system of rice intensification: SRIABSTRACT. The application or the system of rice intensifications (SRI), including seedling and optimalizations and cock secretion fertilizer (CSF) were done. The treatment aim to know the local production level in heavy wet tropic region. The research was conducted in Limau Manis, aside the campus of Andalas University, from July to December 2005 used var. Cisokan. The spilt plot design was applied in the research. The fertilizers were applied according to the recommended 200kg Urea, 200kg SP-36, 150kg KCl. The research variables are plant growth rate, net assimilation ratio, leaf area index, leaf area ratio, shoot/root ratio, tiller number, water use efficiency, and evaporating/ transpiration ratio. The research conclude that the higher the CSF input, the higher the plant vegetative growth. Besides, there were found no different response of 1 to 2 applied seedling, as well as 7 days to 14 days applied seedling with the input.
TINGKAT HASIL DAN KOMPONEN HASIL SISTEM INTENSIFIKASI PADI (SRI) TANPA PUPUK ORGANIK DI DAERAH CURAH HUJAN TINGGI Masdar Masdar; Musliar Kasim; Bujang Rusman; Nurhajati Hakim; Helmi Helmi
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 8, No 2 (2006)
Publisher : Program Studi Ilmu Tanah Fakultas Pertanian UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.8.2.126-131

Abstract

Actually, the system of rice intensifications (SRI) have been encouraging the international researchers, included Indonesians, to intensively increase the yield. The system, such as regulations of planting points, numbers of seedling in each crop point, and planting time were applied here. The research was conducted in Limau Manis, aside the UNAND campus, from January to June 2005 used the varieties of Cisokan. The split plot design was applied in the research. The fertilizers were applied according to the recommended 200 kg urea, 200 kg SP-36, and 150 kg KCl. The research variables are harvest time, productive tillers, numbers of grain per panicle, grain weight, yield component, and yield. The data shows that relatively higher of monthly rainfalls and lower sunny days along the research periods have been the constrains in maximizing the crop production. Simply, the SRI application in the region was significantly useful due to the higher of both crop yield and yield component. 
Growth Response Of SRI Rice In Suboptimal Land To Application Of Cattle Manure And Kieserite Nalwida Rozen; Musliar Kasim; Agustian Agustian; Indra Dwipa
JERAMI Indonesian Journal of Crop Science Vol 2 No 2 (2020): JIJCS
Publisher : Department of Crop Science, Faculty of Agriculture, Andalas University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jijcs.2.2.60-64.2020

Abstract

Population growth rates cause an increase in food demand, but the planting area is decreasing due to land-use change. The land available to be utilized by farmers is mostly suboptimal land. The disadvantages of suboptimal land are low pH values ​​(4.7) and a small number of nutrients (Nitrogen = 0.11%, Phosphorus = 0.45 ppm, very low Carbon, very high Aluminum, and medium Cation exchange capacity) and for increasing the value of suboptimal land can be given organic fertilizer such as cattle manure and kieserite. This study aims to determine the effect of cattle manure and kieserite on rice growth with the SRI planting system in suboptimal land. The study was carried out in farmers' paddy fields at Ambacang Market, Kuranji District, Padang from June to October 2019. This experiment uses a completely randomized design (CRD). Data were analyzed by the F test at a 5% significance level. If it is significantly different, it is continued with the Honestly Significant Difference test (HSD) at the 5% significance level. The results showed that the application of cattle manure and kieserite can increase the height of rice plants and the number of tillers.
MENINGKATKAN KERAGAMAN GENETIK BAWANG PUTIH (Allium sativum L.) MELALUI MUTASI IRRADIASI GAMMA Gusni Yelni; Zulfadly Syarif; Musliar Kasim; P.K. Dewi Hayati
Jurnal Sains Agro Vol 4, No 2 (2019): Jurnal Sains Agro
Publisher : Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (554.431 KB) | DOI: 10.36355/jsa.v4i2.283

Abstract

Physical mutation methods can be used to enhance the character of local varieties of garlic. Gamma irradiation mutation is a good technique for assembling new, vegetatively propagated varieties because the cells are still actively dividing. The aim is to obtain information about the genetic diversity and parentage of certain garlic clones and to determine the level of changes in the gamma.The system used by several Scopus journals, sciencedirect and other sciences that I read is distinct from the gamma irradiation mutations process. The gamma rays used come from the Co-60 or Cs-137 radio nuclides. The dose of the unit is measured in gray (Gy) and is equivalent to 1 J / kg. One kGy= one thousand gy. Rad dose units previously used, where 100 rad= 1 Gy or 1 krad= 10 Gy. And the arbitrary Random Amplification of Polymorphic DNA RAPD. Random Amplified Polymorphism DNA (RAPD) is the dominant marker capable of displaying good polymorphism and can be used to determine genetic diversity and molecular-level relationships among specie.There are important differences in mutations. In morphological, physiological, biological and chemical properties, plant genetic changes occur. Changes can survive as a result of mutations and can develop properly. Normal cells will disappear and mutant cells will develop further, resulting in a new plant appearance. Keywords; garlic clones, mutation induction, gamma rays, and mutagens ABSTRAKPerbaikkan karakter varietas lokal bawang putih bisa dilakukan dengan metode mutasi fisik. Mutasi Iradiasi gamma merupakan teknik yang tepat untuk merakit keragaman baru yang diperbanyak secara vegetatif,  karena sel-selnya masih aktif membelah. Tujuannya untuk memperoleh informasi tentang keragaman dan kekerabatan genetik beberapa klon bawang putih, dan mengetahui tingkat perubahan karakterirtik tanaman bawang putih akibat mutasi irradiasi gamma.Metodenya mutasi iradiasi gamma yang digunakan dari beberapa jurnal Scopus, sciencedirect dan sains lainnya yang saya dibaca berbeda-beda. Sinar gamma yang dipakai berasal dari nuklida radio Co-60 atau Cs-137. Satuan dosis diukur dengan gray (Gy) dan sepadan dengan serapan 1 J/kg. Satu kGy=1000 Gy. Sebelumnya digunakan satuan dosis rad, dimana 100 rad=1 Gy atau 1 krad=10 Gy.  dan marka molekuler Random Amplification of Polymorphic DNA RAPD. Random Amplified Polymorphism DNA (RAPD) merupakan marka dominan yang mampu menunjukkan polimorfisme baik dan bisa digunakan untuk mengetahui keragaman genetik dan hubungan antar spesies pada level molekuler.Mutasi memberikan variasi yang bermanfaat. Perubahan genetik tanaman terjadi pada karakter morfologi, fisiologi, anatomi, dan  kimia. Berubahan akibat mutasi dapat bertahan dan bisa berkembang dengan baik. Sel-sel normal akan menghilang dan sel mutan akan terus berkembang menghasilkan penampilan baru pada tanaman.Kata kunci; klon bawang putih, induksi mutasi, sinar gamma, dan mutagen
Peningkatan Kapasitas Sink pada Tanaman Melon (Cucumis melo L.) dengan Pemberian Giberelin Siti Fatonah; Musliar Kasim; Auzar Syarif
Jurnal Sagu Vol 8, No 02 (2009)
Publisher : Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2176.059 KB) | DOI: 10.31258/sagu.v8i02.1391

Abstract

For cultivation of melon (Cucumis melo L.), farmers only keep one fruit per plant to maintain quality fruit. To improve productivity, one possible effort is by spraying gibberellins at melon in combination with increasing amount of fruit maintained for each plant. The aim of this study was to know the effect of gibbereline in improving sink capacities of melon when fruit amount is increased. Gibberelins was sprayed at concentration of 0, 30, 60, and 90 mg/l to melon crop with different fruit amount per plant (one, two and three fruit). The study indicated that for melon plant with more than one fruit per plant, gibberellins application was not able to maintain the fruit quality, because the fruit weights were still decreasing. Thus, gibberellins application was not able to improve the sink capacities if fruit amount per plant is increased. While for plant with only one fruit, spraying 60 and 90 mg/l gibberellins can improve the fruit weight, so gibberellins improves the sink capacities if only one fruit is maintained for each plant.
PENGARUH KETINGGIAN TEMPAT DAN MUSIM TERHADAP FLUKTUASI APHID PADA TANAMAN KENTANG Hafiz Fauzana; Syafri Syafei; Ahsol Uasyim; Musliar Kasim
Jurnal Sagu Vol 1, No 01 (2002)
Publisher : Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2215.734 KB) | DOI: 10.31258/sagu.v1i01.689

Abstract

The study on the innuence of altitude and season toward aphid population fluctuation inpotato plant. This research was carried out in Rimbo Data, Aia Butunibuak and Sukaraniiareas. Aphid identification was done in Laboratory of Agronomy Study Program, Faculty ofAgriculture, Andalas University, Padang, and in the Research Hall of Fruit Plant, Solok. Theaphid population fluctuation was observed by capturing the aphid flying by installing threetraps of Moerickc tray put in the potato plantation in each area. Treatment of this researchwas in the difference of position altitude of the capture area, viz, Rimbo Data (1,700 mDPL), Aia Batunibuak (1.350 m DPL), and Sukaranii (1.000 in DPL). The research resultshowed that the aphid population in the potato plantation was lower in the area of RimboData (1.700 m DPL) compared with the area of Aia Baturnbuak (1,350 m DPL) and Sukarami(1.000 m DPL). The period witliout aphid was only found in the area of Rimbo Data atOctober, and January, The type of dominant aphid in Rimbo Data was Macrosiphumeiiphorbiae, in Aia Baturnbuak and Sukaranii were Aphis fabae.
Kloning dan karakterisasi gen penyandi inhibitor proteinase dari kulit buah kakao Cloning and characterization of gene encoding proteinase inhibitor of cacao pod wall Mayta Novaliza ISDA; Musliar KASIM; . MANSYURDIN; Tetty CHAIDAMSARI; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 76, No 2: Desember 2008
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.457 KB) | DOI: 10.22302/iribb.jur.mp.v76i2.84

Abstract

Summary Attempts to increase cocoa production in Indonesia have been hinderred by attack of CPB (Conopomorpha cramerella). There has been no effective measures to control this pest leading to development of cacao planting materials which resistant to the pod borer. One of genes functioning in plant defense system against insect pests such as catepilar is Proteinase Inhibitor (PIN). This research aimed to isolate and characterize TcPIN gene of cacao pod wall. A clone of TcPIN was isolated with RT-PCR technique using total RNA of cacao pod wall and DNA primer designed based on the sequence Trypsin Inhibitor of cocoa bean accessible online. BlastX analysis of the sequence of the cDNA clone demonstrated that the ± 600 bp gene cloned with pGEM-T was PIN gene as indicated by highly homologous to Trypsin Inhibitor of Theobroma microcarpum resulted in 248 Score bits and E value 1 e-64. Two sequence alligment with the putative 21 kDa PIN  of cacao seed indicated a moderately high homology. Contrasting these two sequences however found some non identical amino acids implying some variations. Ringkasan Usaha peningkatan produksi kakao di Indonesia terkendala antara lain oleh adanya serangan hama PBK (Conopomorpha cramerella). Untuk menanggulangi serangan PBK tersebut perlu adanya satu cara pengendalian yang efektif dan efisien, sehingga dapat mendorong usaha pengembangan bahan tanam yang tahan PBK. Salah satu gen  membawa sifat ketahanan tanaman terhadap hama ulat adalah Proteinase Inhibitor (PIN). Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi gen TcPIN dari kulit buah kakao. Klon cDNA TcPIN diisolasi dari kulit buah kakao dengan teknik RT-PCR meng-gunakan RNA total kulit buah kakao dan primer DNA yang dirancang atas dasar sekuen Inhibitor Tripsin biji kakao yang diakses lewat internet.  Hasil analisis BlastX dari sekuen klon cDNA menunjukkan  bahwa gen berukuran  ± 600 pb yang telah diklon dengan pGEM-T tersebut adalah PIN karena memiliki homologi yang tinggi terhadap 21 kDa trypsin inhibitor dari Theobroma microcarpum yang meng-hasilkan Skor 248 bits dengan Nilai E 1e-64. Penjajaran dua sekuen dengan PIN putatif 21 kDa yang berasal dari biji kakao menunjuk-kan tingkat homologi yang tinggi, dengan perbedaan nyata sehingga dapat terlihat bahwa keduanya tidak identik.