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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar Jurnal Kedokteran Syiah Kuala e-Journal Pustaka Kesehatan Research Journal of Life Science Indonesian Journal of Biotechnology Journal of Agromedicine and Medical Sciences Mutiara Medika: Jurnal Kedokteran dan Kesehatan UNEJ e-Proceeding MNJ (Malang Neurology Journal) Health Notions Jurnal Pengabdian Pada Masyarakat Warta Pengabdian Multidiciplinary Journal Al-sihah: The Public Health Science Journal Manuju : Malahayati Nursing Journal Majalah Kedokteran Andalas Medical Technology and Public Health Journal Jurnal Inovasi Teknik Kimia Jurnal ABDINUS : Jurnal Pengabdian Nusantara AloHA International Journal of Health Advancement (AIJHA) STRADA Jurnal Ilmiah Kesehatan Jurnal Kesehatan dr. Soebandi
Erma Sulistyaningsih
Department Of Parasitology, Faculty Of Medicine, University Of Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Other

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The Relation between Malondialdehyde (MDA) and Histopatological Appearance in male Wistar Rats Model Fridayanti, Febrina Sylva; Sulistyaningsih, Erma; Sakinah, Elly Nurus
Journal of Agromedicine and Medical Sciences Vol 1, No 1 (2015)
Publisher : Medical Faculty of Jember University (Fakultas Kedokteran Universitas Jember)

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Abstract

Fractures are a serious health problem in Indonesia due to increasing prevalence. The healing process of fracture is disturbed by the oxidative stress that caused by imbalance quantity of free radical and antioxidant. An antioxidant such as polyphenol, which can be found in cocoa, is needed to suppress oxidative stress. The study aimed to investigate the effect of the ethanolic extract of cacao on fracture healing process in a rat model through MDA concentration and histopatological appearance. This study is in vivo experimental study with post-test only controlled group design. 30 male Wistar rats were randomized and divided into 5 groups. 1 group was rats without fractured. The negative control and three treatment groups were rats with fractured manually on left tibia under anesthesia and immobilized by bandage. The treatment groups treated with cocoa ethanolic extract in a dose of 125 mg/kgBW, 250 mg/kgBW, and 500 mg/kgBW orally for 21 days. The result showed that there was a significant different between the treatment groups and the negative control group on MDA concentration and histopatological appearance (p>0,05). The corelation between them were strong and had negative direction (R=-0,771). The study concluded that cocoa ethanolic extract had a positive effect to supress oxidation stress and increases the number of osteoblast on fracture healing process.   Key words: cocoa ethanolic extract, polyphenol, fracture healing process, oxidative stress
Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate Sulistyaningsih, Erma; Moeljopawiro, Sukarti; Subandono, Jarot; Artama, Wayan T.
Indonesian Journal of Biotechnology Vol 10, No 1 (2005)
Publisher : Universitas Gadjah Mada

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Abstract

Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the researchwas to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinanttechnology. Tachyzoite was grown in Balb/c mice in vivo. Messenger RNA was isolated from total RNA and itwas used to synthesis cDNA. Complementary DNA encoding GRA1 protein of tachyzoite Toxoplasma gondii localisolate was amplified and cloned in a prokaryote cloning vector. The recombinant GRA1-encoding gene was thendigesting using EcoRI restriction endonuclease and sequencing. The result showed that the recombinant GRA1-encoding gene consisted of DNA sequences encoding all signal peptide and mature peptide of GRA1 protein.Alignment of recombinant GRA1 sequence to gene encoding GRA1 protein of Toxoplasma gondii RH isolate showed100% homologous.Keywords: GRA1 protein, Toxoplasma gondii, tachyzoite, cloning, cDNA
Specific sequence of Plasmodium falciparum DBL domains associated with severe malaria outcome Sulistyaningsih, Erma; Fitri, Loeki Enggar; Loescher, Thomas; Berens-Riha, Nicole
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Duffy-binding like (DBL) domains of Plasmodium falciparum are believed to be involved in erythrocytes invasion and infected erythrocytes cytoadhesion during the blood stage of malaria infection. In Plasmodium falciparum, DBL domains found in the two different protein families; Erythrocyte Binding Ligand (EBL) including EBA-175, EBA-140, EBA-181 and EBL-1, and Plasmodium falciparum Erythrocyte Membrane Protein-1. The study aimed at investigating the specific sequence of Plasmodium falciparum involved in severe malaria outcome.Blood samples from severe and uncomplicated falciparum malaria cases from Papua and South Kalimantan province, Indonesia, were collected for DNA extraction. A dried blood on filter paper were used for RNA extraction. PCR was performed using UNIEBP primers and directly sequenced. Internal var D primers were designed according to the sequencing of the ~550 bp band produced by UNIEBP primers. The nucleotide sequences were analyzed by NCBI BLAST. Multiple bands ranging from nearly 250 bp to 1 kb were resulted from gDNA in all samples. Two isolates yielded bands of 450 and 525 bp, three isolates showed three bands additionally 250 bp, one isolate presented four bands additionally 800 bp and one isolate resulted one band additionally 1 kb.Amplification of cDNA from severe malaria cases produced one to four bands ranging from 250 bp to 700 bp, and no band observed from cDNA of uncomplicated malaria. Sequencing of the 418 bp bands matched with the eba-175 gene, the 316 bp determined as DBL1a domain and 486 bp band matched with the DBLg domain isolated from placenta of PAM’s Malawian woman. The expression of a 237 bp sequence corresponding to var D gene, was detected solely in severe malaria patients, implicating an association of gene expression and manifestation of severe malaria. Further characterization of the var D gene with a larger sample size is required to draw a definite conclusion. Keywords: DBL domains, Plasmodium falciparum, severe malaria, var D gene.
Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia Arifin, Samsul; Fitri, Loeki Enggar; Sujuti, Hidayat; Hermansyah, Bagus; Endharti, Agustina Tri; Burhan, Niniek; Candradikusuma, Didi; Sulistyaningsih, Erma; Tuda, Josef Sem Berth; Zein, Umar
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.11

Abstract

Indonesia is still included in high endemic area of malaria infection. Early detection as well as appropriate and quick treatment is needed to be able to prevent and treat malaria in Indonesia. Laboratory examination using a microscopic method is still used as the gold standard to diagnose malaria cases. However, the morphology similarity of some Plasmodium species and the number of parasites that can be seen under microscopy causes malaria diagnosis become difficult if only relying on microscopy diagnostic method. The purpose of this study is to analyze the sensitivity and specificity of nested PCR compared to microscopic examination in diagnosing malaria cases. A cross-sectional study has been carried out in some areas of Indonesia and the microscopic analysis as well as nest PCR was done in Laboratory of Parasitology and Laboratory of Central Biomedical Faculty of Medicine, Universitas Brawijaya, Malang East Java Indonesia. A total of 149 blood samples from patients with clinical symptoms of malaria had been obtained from Sumatra, Sulawesi and East Java during December 2011 to December 2013. From 149 sample, 81.9% samples were diagnosed malaria positive by microscopy examination, whereas the PCR results showed that 90.6% of samples were positive. Nested PCR sensitivity is 97.5%, and microscopy 88.2%. Nested PCR specificity is 40.7%, whereas microscopy 78.5%. PPV and NPV for nested PCR are 88,2% and 78.5% respectively, and for microscopy are 97.5% and 40.7% respectively. Nested PCR has a higher sensitivity than microscopy in diagnosing malaria and is able to detect mixed infection better than microscopic examination. However, it is statistically less specific than microscopy examination.
The Density of Dermatophagoides sp. in Households and its Correlation with the Score for Allergic Rhinitis in Jember, East Java Sulistyaningsih, Erma; Laili, Elisa Fadia; Abrori, Cholis
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 21, No 1: January 2021
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v21i1.7925

Abstract

The prevalence of allergic rhinitis (AR) in Indonesia is an estimated 24.3% and increasing each year. The majority cause of AR is house dust mites (HDMs) allergens, especially Dermatophagoides sp. The screening tool to estimate the prevalence and diagnose AF in a population setting is the Score for Allergic Rhinitis (SFAR) questionnaire. This study aimed to investigate the correlation between the density of Dermatophagoides sp. in households and the SFAR in Jember, East Java. The study enrolled 30 housewives as respondents, which were selected by stratified random sampling. Respondents were interviewed using the SFAR questionnaire. The dust sample from each house of the respondent was collected, the HDMs either Dermatophagoides sp. or others were identified and the density of Dermatophagoides sp. was calculated. Data were analyzed using the Pearson Test. Dermatophagoides sp. were found in 21 out of 30 samples and 11 out of 30 respondents had an SFAR of ≥ 7. The Pearson analysis showed the value of p = 0.555 and r = 0.136. Dermatophagoides sp. was the majority of species of HDMs, but there was no significant correlation between the density of Dermatophagoides sp. in households and the SFAR among housewives  in Jember.
THE CIDR1α-PfEMP1 SEQUENCE FROM INDONESIAN PLASMODIUM FALCIPARUM AND ITS POTENTIAL ASSOCIATION WITH THE CEREBRAL OUTCOME Sulistyaningsih, Erma; Armiyanti, Yunita; Dewi, Rosita
Malang Neurology Journal Vol 7, No 1 (2021): January
Publisher : Malang Neurology Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.mnj.2021.007.01.8

Abstract

Background: Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) is an important protein responsible for the pathogenesis of severe malaria, including cerebral malaria. The protein is highly diverse. The CIDR1α-PfEMP1 binds endothelial protein receptor (EPCR) and may associated with the brain swelling in childhood malaria.Objective: To analyze the CIDR1α-PfEMP1 from Indonesian isolate and determine its association with cerebral malaria outcome.Methods: Fifteen blood samples of clinically mild to severe malaria-patient were collected for DNA extraction. Malaria diagnosis was conducted microscopically by Giemsa-stained thin blood smear. The CIDR1α domain was amplified by PCR using specific primer and PCR product was sequenced. The nucleotide sequences were analyzed by NCBI blast, DNASIS MAX 3 and translated into amino acid sequences using Expasy Translation Tool.Results: One out of fifteen samples was severe malaria case and infected with P.  falciparum, the rest were clinically mild to moderate malaria and infected with pure P. falciparum or mixed infection of P. falciparum and P. vivax. Amplification for CIDR1α domain resulted a single band of + 550 bp from a severe sample only. Sequencing of PCR product on both strands read 524 nucleotides and BLAST analysis confirmed as CIDR1α sequence. Multiple alignment showed 74-78% nucleotide sequence similarity with reference sequences, but amino acid sequences presented 23.5% homologous.Conclusion: An identified CIDR1α domain only from severe case implicating the potential association with the severe outcome including cerebral malaria, but the highly diverse of the domain needs further studies on the interaction with the pathological-causing receptor in the host. 
The Density of Dermatophagoides sp. in Households and its Correlation with the Score for Allergic Rhinitis in Jember, East Java Sulistyaningsih, Erma; Laili, Elisa Fadia; Abrori, Cholis
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 21, No 1 (2021): January
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v21i1.7925

Abstract

The prevalence of allergic rhinitis (AR) in Indonesia is an estimated 24.3% and increasing each year. The majority cause of AR is house dust mites (HDMs) allergens, especially Dermatophagoides sp. The screening tool to estimate the prevalence and diagnose AF in a population setting is the Score for Allergic Rhinitis (SFAR) questionnaire. This study aimed to investigate the correlation between the density of Dermatophagoides sp. in households and the SFAR in Jember, East Java. The study enrolled 30 housewives as respondents, which were selected by stratified random sampling. Respondents were interviewed using the SFAR questionnaire. The dust sample from each house of the respondent was collected, the HDMs either Dermatophagoides sp. or others were identified and the density of Dermatophagoides sp. was calculated. Data were analyzed using the Pearson Test. Dermatophagoides sp. were found in 21 out of 30 samples and 11 out of 30 respondents had an SFAR of ≥ 7. The Pearson analysis showed the value of p = 0.555 and r = 0.136. Dermatophagoides sp. was the majority of species of HDMs, but there was no significant correlation between the density of Dermatophagoides sp. in households and the SFAR among housewives  in Jember.
The Effect of Pesticides Usage Behaviour on General Health Conditions of Farmer in Jember Regency, Indonesia Salim Salim; Erma Sulistyaningsih; Ristya Widi Endah Yani
Health Notions Vol 4, No 1 (2020): January
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.407 KB) | DOI: 10.33846/hn40102

Abstract

The agricultural sector remains an important sector in Indonesia's economic development, and pesticides have been essential in agricultural practice to protect crops and increase crop production. However, pesticides can cause a negative impact on health as well as environmental. The purpose of this study was to determine the effect of pesticides usage behavior on farmers' general health conditions. This was an observational analytic study with cross sectional design. The respondent was 377 farmers selected by cluster sampling. The variable of knowledge, attitude and practice were analysed. Data analysis showed that there was a significant effect on the level of knowledge on the farmers' general health conditions with a p-value of 0.001. As much as 47.2% of respondents had a low level of knowledge about the use of pesticides. The attitude influenced the general health conditions of farmers with p-value of 0,000. As much as 52.5% of respondents had a positive attitude related to pesticides and 47.5% had a negative attitude concerning pesticides usage. The variable of practice on the use of pesticides significantly affected the general health conditions of farmers with a p-value of 0.003. And as much as 17.0% respondents showed a bad practice, 65.5% moderate practice and 17.5% good practice on pesticides. Keywords: attitude; health; knowledge; practice; pesticide
Behavioral Factors Against Hepatitis A Incidence in Universitas Jember with Lawrence Green Method Eva Sulawati Arum; Sri Hernawati; Erma Sulistyaningsih
Health Notions Vol 5, No 01 (2021): January
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn50103

Abstract

Hepatitis A is an inflammatory liver disease caused by Hepatitis A virus is Ribonucleic Acid (RNA) Virus. Hepatitis A viruses belonging to the family picornaviridae, genus hepatovirus, have 1 serotype and 4 genotypes. Outbreak of hepatitis A in the last 4 years, often occurs among students, especially students of the Universitas Jember. The results of observations made by researchers in September 2020, namely the level of knowledge of students of Universitas Jember was still less and could be more at risk of contracting hepatitis A compared to students who were well knowledgeable. The results of the data analysis showed that the level of knowledge of Universitas Jember students about hepatitis A could be more at risk of contracting hepatitis A compared to students who were well-knowledgeable. Knowledge of hepatitis A will certainly support the change in health behaviors, namely behaviors related to preventive, curative and rehabilitative efforts against hepatitis A. In general, students' knowledge of hepatits A was still half of respondents lack good knowledge of hepatitis A. Supportive behaviors in improving health are needed means to disseminate information about hepatitis A which includes preventive, curative and rehabilitative efforts. A person's knowledge of the disease is very important for the formation of actions that will be carried out by the person, because with good knowledge can create good actions. Keywords: behavior; hepatitis A; student
HUBUNGAN KEPADATAN DERMATOPHAGOIDES SPP. DENGAN FREKUENSI SERANGAN ASMA PADA PENDERITA ASMA DI RS PARU JEMBER Denaneer Rahmadatu; Erma Sulistyaningsih; Dini Agustina
Jurnal Kedokteran Syiah Kuala Vol 19, No 2 (2019): Volume 19 Nomor 2 Agustus 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v19i2.18058

Abstract

Abstrak. Asma merupakan gangguan inflamasi kronik saluran napas yang bersifat hiperesponsif yang ditandai adanya gejala episodik berulang berupa mengi, sesak napas, dada terasa berat, dan batuk, terutama pada malam hari atau dini hari. Sekitar 50-80% asma disebabkan oleh tungau debu rumah (TDR), khususnya Dermatophagoides pteronyssinus dan Dermatophagoides farinae. Penelitian ini bertujuan untuk mengetahui apakah terdapat hubungan antara kepadatan Dermatophagoides spp. pada debu rumah dengan frekuensi serangan asma pada penderita asma di RS Paru Jember. Penelitian ini merupakan penelitian analitik observasional dengan desain penelitian cross-sectional. Sampel penelitian ini yaitu 30 penderita asma di RS Paru Jember yang diambil menggunakan teknik simple random sampling sesuai dengan kriteria inklusi dan eksklusi. Peneliti melakukan interview mengenai frekuensi serangan asma dalam tiga bulan terakhir, mengambil debu dari ruang tamu dan ruang tidur pada rumah penderita asma, dan mengidentifikasi tungau debu rumah (TDR) yang ditemukan pada debu. Hasil uji Pearson mendapatkan nilai p=0,011 dan nilai r=0,475 sehingga dapat disimpulkan bahwa terdapat hubungan bermakna antara kepadatan Dermatophagoides spp. dengan frekuensi serangan asma dengan kekuatan korelasi sedang pada penderita asma di RS Paru Jember. Kata Kunci: Dermatophagoides spp., Tungau Debu Rumah (TDR), Asma Abstract. Asthma is a chronic inflammatory disorder of the respiratory system characterized by recurrent episodes of wheezing, shortness of breath, chest tightness, and cough, particularly at night or early morning. About 50-80% of asthma is caused by house dust mite (HDM), especially Dermatophagoides pteronyssinus and Dermatophagoides farinae. This study aimed to investigate the correlation between the density of Dermatophagoides spp. and the frequency of asthma attacks in asthmatic patients at Jember Pulmonary Hospital. This study was an observational analytic study with a cross-sectional design. Respondents were 30 asthmatic patients at Jember Pulmonary Hospital matched with inclusion and exclusion criteria and chose by simple random sampling. Respondents were interviewed about the frequency of asthma attacks in the last three month,s and the hose dust from the living and sleeping rooms were collected for identification of house dust mites (HDM). The pearson test showed a value of p=0.011 and r=0.475. It can be concluded that there is a significant correlation with the moderate strenght between the density of Dermatophagoides spp. and the frequency of asthma attacks in the asthmatic patient at Jember Pulmonary Hospital. Keywords: Dermatophagoides spp., House Dust Mites (HDM), Asthma
Co-Authors Agustina Tri Endharti Al Munawir Aldi Cahya Muhammad Alfi Kamalia Aliyatul Farida Anas Bakhtiar Diyansah Ancah Caesarina Novi Marchianti Bagus Hermansyah Bagus Hermansyah Burhan, Niniek Cholis Abrori, Cholis Cicih Komariah Denaneer Rahmadatu Devita Prima Nurmasari Didi Candradikusuma Didi Candradikusuma Dini Agustina Dion Krismashogi Darmawan Dwi Wahyuni Dwita Aryadina Rachmawati Elly Nurus Sakinah, Elly Nurus Endang Sri Purwanti Wulandari Eqiel Navadz Akthar Alami Erfan Efendi Eva Sulawati Arum Fathul Hidayatul Hasanah Febrina Sylva Fridayanti, Febrina Sylva Fifil Sumarni Sumarni Firdha Novitasari Fitri Dika Maharani FX Ady Soesetijo, FX Ady Ghani Silahudin Hadi Prayitno Heni Fatmawati Hidayat Sujuti I Ketut Suada Ika Rahmawati Sutejo Intan Rachmawati Irawan Fajar Kusuma Iza Billa Fahmi Jarot Subandono Jarot Subandono Jauhar Firdaus Josef Sem Berth Tuda Kahfi Karunia Ilahi Laeli Kurniasari Laili, Elisa Fadia Laily Rahmawati Leersia Yusi Ratnawati Loeki Enggar Fitri Luh Putu Ratna Sundari Muhammad Hasan Ngurah Agung Reza Satria Nugraha Putra Nicole Berens-Riha Nicole Berens-Riha, Nicole Niniek Burhan Nur Aqmarina Kusumawardani Pralampita, Pulong Wijang Pungky Setya Arini Rina Fitriana Rina Fitriana Ristya Widi Endah Yani Rosita Dewi Salim Salim Samsul Arifin Samsul Arifin Sheilla Rachmania Silvi Ahmada Chasya Sinta Ariyani Sri Hernawati Sugiyanta Sugiyanta Sukarti Moeljopawiro Sukarti Moeljopawiro Thomas Loescher Thomas Loescher, Thomas Tristira Urvina Tuda, Josef Sem Berth Umar Zein Warda Ayu Nadira Wayan T. Artama Wayan T. Artama Widhi Dyah Sawitri Wiji Utami Winnie Almira Setyoadji Wiwien Sugih Utami Yunita Armiyanti