Bagus Hermansyah
Departemen Parasitologi, Fakultas Kedokteran, Universitas Jember, Jl. Kalimantan No.37, Kampus Tegal Boto, Kabupaten Jember, Jawa Timur, 68121

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Bioactivity of a Compound of Standardized Bangle (Zingiber cassumunar Roxb.) Extract Fraction as a Complimentary Therapy to Prevent Malaria Complications Hermansyah, Bagus; Utami, Wiwien Sugih
Journal of Agromedicine and Medical Sciences Vol 1, No 2 (2015)
Publisher : Medical Faculty of Jember University (Fakultas Kedokteran Universitas Jember)

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Abstract

Malaria is still a major problem in the international and national scale. World Health Organization (WHO) states that based on World malaria report, world cases of malaria reached 216 million and an estimated of 655 thousand people died, in 2010. Overproduction of pro-inflammatory cytokines by phagocytic cells and activated endothelial cells occurs In the severe malaria. Bangle (Zingiber cassumunar Roxb.) has the potential to be developed as a new complementary therapy that is expected to prevent fatal malaria complications. The research objective is to test in vivo, the activity of a compound of standardized Bangle (Zingiber cassumunar Roxb.) fraction extract as a complementary therapy along with standard anti-malarial drug, artemisin. This is a true experimental study using twenty-five mice Balb / C male which are divided into five groups; the negative control group, positive control group treated KI, II, and III stimulation with each of the compounds of standardized fraction extract of bangle ( FEBT-h, FEBT-dcm, FEBT-m) for 14 days. Oneway ANOVA test showed the result of p = 0.01 (p <0.05). Continued with LSD test that showed the most significant in lowering the degree of parasitaemia is the group of methanol (FEBT-m).Keywords: Zingiber cassumunar Roxb, fractionated extracts, complementary therapies, malaria
Uji Aktivitas Fraksi N-Heksana Ekstrak Metanol Bangle (Zingiber cassumunar Roxb.) sebagai Terapi Komplementer Malaria secara In Vivo (The Activity Test of N-Hexane Fraction of Bangle (Zingiber cassumunar Roxb.) Methanolic Extract as Complementary Thera Sarah Andriani; Bagus Hermansyah; Sugiyanta Sugiyanta
Pustaka Kesehatan Vol 5 No 2 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Malaria is one of endemic disease in Indonesia that caused many deaths. Immune system plays important rules in preventing malaria severity. Bangle has been proven as an immunostimulant and could reduce parasitaemia in malaria infection. This study aimed to determine the activity of n-hexane fraction of bangle methanolic extract as complementary therapy of malaria. This study used 28 mice, divided into one positive control group, one negative control group, and five treatment groups. Bangle stimulation before induction was given to five treatment groups with dose: 0,005625mg/grbw, 0,01125mg/grbw, 0,0225mg/grbw, 0,045mg/grbw, and 0,09mg/grbw for 14 days. Plasmodium berghei induction was given to all groups, and then treatment groups were given ACT and bangle fraction, and positive control group was given ACT without fraction for three days. Parasitaemia was observed everyday, then plasmodium growth inhibition percentation was counted. Bangle fraction stimulation gave lower parasitaemia at the begining and Pearson correlation test showed strong positive correlation between dose of bangle fraction and plasmodium growth inhibition percentation (p<0,01). We can conclude that n-hexane fraction of bangle methanolic extract was active as complementary therapy of malaria (IC50=0,013 mg/grbb). Keywords: malaria, bangle, n-hexane, complementary therapy
Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia Arifin, Samsul; Fitri, Loeki Enggar; Sujuti, Hidayat; Hermansyah, Bagus; Endharti, Agustina Tri; Burhan, Niniek; Candradikusuma, Didi; Sulistyaningsih, Erma; Tuda, Josef Sem Berth; Zein, Umar
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.11

Abstract

Indonesia is still included in high endemic area of malaria infection. Early detection as well as appropriate and quick treatment is needed to be able to prevent and treat malaria in Indonesia. Laboratory examination using a microscopic method is still used as the gold standard to diagnose malaria cases. However, the morphology similarity of some Plasmodium species and the number of parasites that can be seen under microscopy causes malaria diagnosis become difficult if only relying on microscopy diagnostic method. The purpose of this study is to analyze the sensitivity and specificity of nested PCR compared to microscopic examination in diagnosing malaria cases. A cross-sectional study has been carried out in some areas of Indonesia and the microscopic analysis as well as nest PCR was done in Laboratory of Parasitology and Laboratory of Central Biomedical Faculty of Medicine, Universitas Brawijaya, Malang East Java Indonesia. A total of 149 blood samples from patients with clinical symptoms of malaria had been obtained from Sumatra, Sulawesi and East Java during December 2011 to December 2013. From 149 sample, 81.9% samples were diagnosed malaria positive by microscopy examination, whereas the PCR results showed that 90.6% of samples were positive. Nested PCR sensitivity is 97.5%, and microscopy 88.2%. Nested PCR specificity is 40.7%, whereas microscopy 78.5%. PPV and NPV for nested PCR are 88,2% and 78.5% respectively, and for microscopy are 97.5% and 40.7% respectively. Nested PCR has a higher sensitivity than microscopy in diagnosing malaria and is able to detect mixed infection better than microscopic examination. However, it is statistically less specific than microscopy examination.
PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Mufida, Diana Chusna; Salsabila, Yuna Annisa; Suswati, Enny; Hermansyah, Bagus; Agustina, Dini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3930

Abstract

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 
Uji Aktivitas Fraksi Diklorometana Ekstrak Metanol Bangle (Zingiber cassumunar Roxb.) sebagai Terapi Komplementer Malaria secara In Vivo (The Activity Test of Dichloromethane Fraction of Bangle (Zingiber cassumunar Roxb.) Methanolic Extract as Complement Edda Rachmadenawanti; Bagus Hermansyah; Yuli Hermansyah
Pustaka Kesehatan Vol 4 No 2 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

World Malaria Report reported that Malaria threats 40% of world population in six regions and ninety seven endemic countries. This study aimed to investigate the activity of dichloromethane fraction of Bangle as a complementary therapy of malaria in vivo. This was true experimental laboratories study using 28 mice of Balb/c, divided into seven groups. Five treatment groups were given five dose of dichloromethane fraction of Bangle methanolic extracts as imunostimulant before malaria infection and during malaria therapy by combining with malaria standard therapy. K(+) group was given malaria standard therapy. K(-) group was not given any therapy after infection. Inhibition percentage of malaria parasite in K(-) was 0 and K(+) was 24,74%. The treatment group of 0,09 mg/grBW had the highest inhibition percentage, as many as 88,19%. It declined by decreasing dose of fraction. The result of pearson correlation showed very strong correlation (r=0,880). The Ic50 value of dichloromethane fraction of Bangle methanolic extract as a complementary therapy was 0,008 mg/grBW. In conclusion, dichloromethane fraction of Bangle methanolic extract had immunostimulant effect and antimalarial activity in vivo.   Key words: bangle, complementary therapy, inhibition percentage, malaria
MODULASI AKTIVITAS CIPROFLOXACIN TERHADAP Pseudomonas aeruginosa OLEH N-ASETILSISTEIN DAN VITAMIN C Agustina, Dini; Indreswari, Laksmi; Tristianti, Farmitalia Nisa; El Milla, Kardiana Izza; Hermansyah, Bagus; Wahyudi, Septa Surya; Firdaus, Jauhar
Syifa'Medika Vol 11, No 1 (2020): Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan
Publisher : Faculty of Medicine

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/sm.v11i1.2389

Abstract

Pseudomonas aeruginosa merupakan salah satu bakteri penyebab infeksi yang sulit diobati dengan terapi obat tunggal karena tingkat keberhasilan yang rendah serta kecenderungan menjadi resisten selama pemberian obat tunggal. Salah satu antibiotik yang digunakan adalah ciprofloxacin. Untuk meningkatkan sensitivitas P. aeruginosa terhadap ciprofloxacin, kombinasi N-asetilsistein atau vitamin C mungkin diperlukan. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi antara N- asetilsistein dan ciprofloxacin serta antara vitamin C dan ciprofloxacin terhadap pertumbuhan P. aeruginosa. Penelitian ini menggunakan uji difusi cakram yang berisi kombinasi konsentrasi ciprofloxacin konstan 1mg/ml dengan N-asetilsistein dalam berbagai konsentrasi 1,25mg/ml, 2,5mg/ml, 5mg/ml, 10mg/ml, dan 20mg/ml dan vitamin C dengan 2,5 mg/ml; 5 mg/ml; 10 mg/ml; 20 mg/ml; dan konsentrasi 40 mg/ml. Dalam kombinasi antara N-asetilsistein dan ciprofloxacin, peningkatan sensitivitas P. aeruginosa terjadi pada konsentrasi 10ml/mg pada kombinasi obat, dan dalam kombinasi antara vitamin C dan ciprofloxacin, peningkatan sensitivitas P. aeruginosa terjadi pada konsentrasi 2, 5 ml/mg pada kombinasi obat di atas kontrol positif. Regresi logaritmik mengungkapkan konsentrasi minimal N- asetilsistein dan vitamin C masing-masing adalah 9,593 mg/ml dan 1,9 mg/ml dapat meningkatkan sensitivitas P. aeruginosa pada ciprofloxacin. Dapat disimpulkan bahwa N-asetilsistein dan vitamin C meningkatkan aktivitas ciprofloxacin untuk menghambat pertumbuhan P. aeruginosa in vitro.
Hubungan antara Higiene Perorangan dan Kejadian Koinfeksi Cacingan pada Penderita Tuberkulosis Shofia, Lailatis; Hermansyah, Bagus; Suswati, Enny; Agustina, Dini; Mufida, Diana Chusna; Shodikin, Muhammad Ali
Sriwijaya Journal of Medicine Vol. 4 No. 1 (2021): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/SJM.v4i1.153

Abstract

Tuberculosis (TB) and intestinal worms are infectious diseases with a large number of sufferers in Indonesia. The high prevalence of intestinal worms in Indonesia allows the occurrence of STH coinfection in pulmonary TB patients which causes anti M. tuberculosis immunity to decrease so that the response to tuberculosis treatment is not optimal. One of the risk factors of STH infection is personal hygiene. The purpose of this study was to analyze the relationship between individual hygiene and the incidence of STH coinfection in TB patients in Tempurejo Sub-District, Jember District. This type of research is observational research with cross sectional analytic design. The population used was TB patients in Tempurejo Sub-District in the period September - October 2019. The data used in this study were primary data in the form of interviews and results of stool examination. Stool examination is carried out using the sedimentation and floatation methods. Data analysis was performed using Fisher's exact test. The results of this study indicate that the incidence of STH co-infection in TB patients in Tempurejo Subdistrict is 9.67% and is caused by two STH species, A. lumbricoides (66.7%) and Hookworm (33.3%). Respondents' personal hygiene consisted of good hygiene (64.5%) and bad hygiene (35.5%), where 66.7% of cases of STH coinfection occurred in respondents with poor personal hygiene. Fisher's test results showed that there was no significant relationship between personal hygiene with the incidence of STH coinfection in TB patients in Tempurejo Sub-District, Jember District (p-value of 0.281).
Hubungan antara Higiene Perorangan dan Kejadian Koinfeksi Cacingan pada Penderita Tuberkulosis Shofia, Lailatis; Hermansyah, Bagus; Suswati, Enny; Agustina, Dini; Mufida, Diana Chusna; Shodikin, Muhammad Ali
Sriwijaya Journal of Medicine Vol. 4 No. 1 (2021): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/SJM.v4i1.153

Abstract

Tuberculosis (TB) and intestinal worms are infectious diseases with a large number of sufferers in Indonesia. The high prevalence of intestinal worms in Indonesia allows the occurrence of STH coinfection in pulmonary TB patients which causes anti M. tuberculosis immunity to decrease so that the response to tuberculosis treatment is not optimal. One of the risk factors of STH infection is personal hygiene. The purpose of this study was to analyze the relationship between individual hygiene and the incidence of STH coinfection in TB patients in Tempurejo Sub-District, Jember District. This type of research is observational research with cross sectional analytic design. The population used was TB patients in Tempurejo Sub-District in the period September - October 2019. The data used in this study were primary data in the form of interviews and results of stool examination. Stool examination is carried out using the sedimentation and floatation methods. Data analysis was performed using Fisher's exact test. The results of this study indicate that the incidence of STH co-infection in TB patients in Tempurejo Subdistrict is 9.67% and is caused by two STH species, A. lumbricoides (66.7%) and Hookworm (33.3%). Respondents' personal hygiene consisted of good hygiene (64.5%) and bad hygiene (35.5%), where 66.7% of cases of STH coinfection occurred in respondents with poor personal hygiene. Fisher's test results showed that there was no significant relationship between personal hygiene with the incidence of STH coinfection in TB patients in Tempurejo Sub-District, Jember District (p-value of 0.281).
Environmental Sanitation, Personal Hygiene, STH Co-infection in TB Patients Fitri, Nadya Eka; Mufida, Diana Chusna; Hermansyah, Bagus; Armiyanti, Yunita; Agustina, Dini; Suswanti, Enny
KEMAS: Jurnal Kesehatan Masyarakat Vol 16, No 3 (2021)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v16i3.23639

Abstract

Globally, helminthiasis is one of the infectious diseases that are often associated with the incidence of tuberculosis. Helminth co-infection modulates the immune system of TB patients by reducing Th-1 response that functions as protector against Mycobacterium tuberculosis. This condition disrupts the process of eliminating bacteria so that its development becomes more progressive. The impact is the activation of latent TB and the success of TB treatment. A Soil-transmitted Helminth (STH) is a group of intestinal worms that often infect humans. Previous studies prove that environmental sanitation and personal hygiene are risk factors associated with STH infection. The study to determine the relationship of environmental sanitation and personal hygiene with the incidence of STH co-infection in TB patients at Puskesmas Puger, Jember in September until December 2019. This study uses a cross-sectional design. The sampling technique uses total sampling. A total of 32 TB patients met the criteria as study sample. The data analysis uses Fisher Test. Results showed the prevalence of STH co-infection was relatively low (18.8%); most of the respondents had good environmental sanitation and personal hygiene conditions. Bivariate analysis showed p-value (0,476) for environmental sanitation and p-value (1,000) for personal hygiene. This study concludes that there is no correlation between environmental sanitation, personal hygiene, and STH co-infection in TB patients at Public health center in Puger.
Interleukin-22-induced β‑defensin-2 expression by intranasal immunization with Streptococcus pneumoniae RrgB epitopes Mufida, Diana Chusna; Saputra, Antonius Dwi; Hermansyah, Bagus; Agustina, Dini; Shodikin, Muhammad Ali; Armiyanti, Yunita
Universa Medicina Vol. 41 No. 1 (2022)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2022.v41.37-46

Abstract

BackgroundStreptococcus pneumoniae causes pneumococcal disease, which is responsible for millions of deaths worldwide. Various pneumococcal vaccine candidates have been developed to prevent S. pneumoniae infection, one of which is an epitope-based vaccine. This study aimed to prove that intranasal immunization with each of the five S. pneumoniae RrgB epitopes can induce a mucosal immune response by increasing the β-defensin-2 concentration through upregulation of interleukin (IL)-22 expression. MethodsAn experimental laboratory study was conducted using 28 male Wistar rats aged 3-4 months, that were randomly divided into 7 groups containing four rats each. Group 1 was given 40 mL of phosphate-buffered saline (PBS) only (control group). Group 2 was the adjuvant group that received 40 mL PBS containing 2 ìg cholera toxin B (CTB), and groups 3-7 were immunized with 40 mL PBS containing a combination of adjuvant and one of the five different S. pneumoniae RrgB epitopes. The concentrations of IL-22 and β-defensin-2 from nasal rinse examination were measured by means of ELISA. The Kruskal-Wallis test, followed by the Mann-Whitney post-hoc test were used for statistical analysis. ResultsRats immunized with the adjuvant-epitope combination had significantly higher β-defensin-2 and IL-22 levels than the control group (p=0.030; p=0.018, respectively), according to the Kruskal-Wallis test. And the Mann-Whitney statistical test, showed there was a significant increase in β-defensin-2 and IL-22 levels. ConclusionsIntranasal immunization with epitope 1 of the S. pneumoniae RrgB can increase β-defensin-2 expression significantly and has a greater potential to be developed into a pneumococcal vaccine.