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All Journal Jurnal Sain Veteriner BERITA BIOLOGI FITOFARMAKA: Jurnal Ilmiah Farmasi EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Molekul: Jurnal Ilmiah Kimia Charity : Jurnal Pengabdian Masyarakat International Journal of Business, Economics, and Social Development KENDURI
Oom Komala
Universitas Pakuan
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Social Sciences Veterinary

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UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN EKSTRAK AIRKELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L) TERHADAP BAKTERI Streptococcus pneumoniae. Komala, Oom; Rosyanti, Reni; Muztabadihardja, Muztabadihardja
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 3, No 1 (2013): FITOFARMAKA
Publisher : Universitas Pakuan

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Abstract

Penelitian efektivitas antibakteri ekstrak etanol dan ekstrak air kelopak bunga rosella terhadap Streptococcus pneumoniae telah dilakukan menggunakan metode difusicakram dan dilusi secara invitro. Larutan induk ekstrak etanol dan ekstrak air rosella70g/100 ml diencerkan dengan konsentrasi 10%, 30%, 50% dan70% untuk metodedifusi cakram dengan kontrol positif ampisilin 10 IU, dan ekstrak etanol diencerkandengan konsentrasi 0,1%, 0,25%, 0,5%, 1%, dan 2% untuk metode dilusi terhadapS.pneumoniae. Hasil penelitian menunjukkan bahwa ekstrak etanol dan ekstrak airrosella dapat menghambat pertumbuhan bakteri S.pneumoniae diketahui dari zonahambat yang terbentuk. Konsentrasi 70% pada metode difusi ekstrak etanol danekstrak air rosella membentuk diameter zona hambat paling luas rata-rata 25,6 mmuntuk ekstrak etanol dan 24,3 mm untuk ekstrak air rosella. Hasil konsentrasi hambatminimum dari ekstrak etanol rosella yang paling efektif adalah konsentrasi 1%. Hasilpengujian fitokimia diketahui bahwa ekstrak etanol dan ekstrak air rosellamengandung saponin, tanin, dan flavonoid.Kata kunci : Kelopak Bunga Rosella (Hibiscus sabdariffa L), S.pneumoniae, antibakteri, ekstrak etanol, ekstrak air
UJI EFEKTIVITAS EKSTRAK ETANOL DAUN LIDAH MERTUA (Sansevieria trifasciata Prain) TERHADAP KHAMIR Candida albicans Komala, Oom; Yulia, Ike; Pebrianti, Rita
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 2, No 2 (2012): FITOFARMAKA
Publisher : Universitas Pakuan

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Abstract

Lidah mertua (Sansevieria trifasciata Prain) merupakan tanaman yang berasal dariAfrika dan dikenal sebagai antimikroba, serta berkhasiat obat. Tujuan dari penelitian ini ialah mengetahui kandungan antimikroba ekstrak daun lidah mertua dengan menentukan lebar daerah hambat (LDH) terhadap khamir Candida albicans menggunakan metode difusi kertas cakram. Pengujian LDH dilakukan terhadap konsentrasi ekstrak daun lidah mertua 60%, 70% , 80%, 90%, serta ketokonazol 14 ppm sebagai kontrol positif dan karboksi metil selulosa (CMC) 0,5% sebagai kontrol negatif. Hasil penelitian menunjukkan bahwa ekstrak daun lidah mertua pada konsentrasi 90% membentuk zona hambat terhadap C. albicans yang paling luas tetapi tidak jernih. Hasil analisis mutu ekstrak diketahui bahwa kadar abu ekstrak daun lidah mertua yang tidak larut dalam asam ialah 0,23% dan yang larut dalam air ialah 5,04%. Sedangkan hasil penetapan kadar sari ekstrak daun lidah mertua yang larut dalam air ialah 38,76% dan yang larut dalam etanol ialah 12,53%. Hasil fitokimia diketahui ekstrak daun lidah mertua mengandung saponin, flavonoid, steroid, dan triterpenoid, yang berfungsi dapat menghambat C. albicans.Kata kunci : daun lidah mertua (Sansevieria trifasciata Prain), Candida albicans, efektivitas, antikhamir
UJI EFEKTIVITAS EKSTRAK ETANOL BUAH PARE (Momordica charantia L.) SEBAGAI ANTIBAKTERI Salmonella typhi Komala, Oom; Lohita Sari, Bina; Sakinah, Nina
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 2, No 1 (2012): FITOFARMAKA
Publisher : Universitas Pakuan

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Abstract

Penelitian yang bertujuan untuk mengetahui efektivitas antibakteri dari beberapa konsentrasi ekstrak etanol buah pare terhadap bakteri Salmonella typhi telah dilakukan. Pengujian antibakteri ekstrak etanol buah pare dilakukan dengan mengukur Diameter Daerah Hambat (DDH) melalui metode difusi kertas cakram dan Konsentrasi Hambat Minimum (KHM) melalui metode dilusi agar padat. Pengujian DDH dilakukan terhadap konsentrasi ekstrak buah pare 12,5%, 25%, 50%, 75%, serta kloramfenikol 30 UI sebagai kontrol positif dan aquadest sebagai kontrol negatif. Sedangkan pengujian KHM dilakukan terhadap konsentrasi 10%, 20%, 30%, 40%, 50%, 60%, 70% dan 80%. Hasil penelitian menunjukkan bahwa ekstrak etanol buah pare kurang efektif sebagai antibakteri terhadap Salmonella typhi, karena pada pengujian DDH zona hambat yang terbentuk tidak absolut. KHM berada pada konsentrasi 60%, dimana tidak ada pertumbuhan bakteri. Kata kunci: Buah pare, Salmonella typhi, efektivitas antibakteri 
UJI ANTIBAKTERI SEDIAAN OBAT KUMUR EKSTRAK DAUN PANDAN WANGI (Pandanus amaryllifolius Roxb) TERHADAP Streptococcus mutans Komala, Oom; Nur’aini, Putri; Indriati, Dwi
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Publisher : Fakultas Matematika dan ilmu pengetahuan Alam. Universitas Pakuan.

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Abstract

Pandanus amaryllifolius Roxb leaf  has potential as an antibacterial.  The research aims to know the ability of stocks to mouthwash scented pandan leaf extract inhibits Streptococcus mutans and in determining the fragrant pandanus phytochemicals compounds. Wide-Area Drag testing (LDH) mouthwash extracts was performed with a concentration of 15%, 17.5% and 20%. Test the phytochemical saponins, alkaloids, tannins and flavonoids qualitatively on 0.5 g powder.  Testing stabilita include organoleptik observations of color, aroma, taste preparation, test the pH, specific gravity and viscosity. The results showed that the material of pandanus leaf extract mouthwash scented antibacterial nature against Streptococcus mutans with a concentration of extracts of 15%, 17.5% and 20% showed values of LDH consecutive 16.3 mm; 22.6 mm and 28.3 mm earlier in the week while in week 8 loss to the value of LDH is 12.5 mm; 15.5 mm; and 19.6 mm.  The active compounds in the leaves of pandanus is alkaloids, saponins, tannins and flavonoids.  Mouthwashes stored at 25-30 ° C for 8 weeks are relatively stable for color, flavor, taste, pH, specific gravity, and viscosity, compared to a temperature of 40oC. Key words: Pandan fragrance, Streptococcus mutans, mouthwash
AKTIVITAS DEGRADASI SAMPAH ORGANIK DALAM BIOPORI Wiedarti, Sri; Lubis, M. Akhmar Yusfi; Komala, Oom
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Publisher : Fakultas Matematika dan ilmu pengetahuan Alam. Universitas Pakuan.

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Abstract

Biopori is pore space or in the ground formed by living creatures, such as microorganisms of the soil and plant roots. The shape resembles biopori hole in the ground and forked branches and very effective to channel water and air into the soil. In order to make the hole biopori still function optimally it should be routine coupled with organic material, so in the hole biopori absorption will still take place in aerobic composting process by soil microorganisms.The addition of microbes in accelerating the process of degradation of the garbage that is by adding a inoculum composite comprising four microorganism Saccharomyces,  Lactobacillus, Acetobacter sp., and Bacillus. The addition of the best treatment with the addition of inoculum and soil, have physical characteristics that resemble the physical characteristics of the soil. Key words: Biopori, organic waste, microbes
UJI EFEKTIVITAS ANTIBAKTERI PERASAN SEGAR DAN SERBUK UMBI BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI Salmonella typhi Komala, Oom; Asmara, Herra Indra; Wiendarlina, Ike Yulia
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Publisher : Fakultas Matematika dan ilmu pengetahuan Alam. Universitas Pakuan.

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Abstract

Herbs and spices are very important and useful as therapeutic agent against typhoid fever. Increasing multidrug resistance of pathogens forces to find alternative compounds for treatment of these infectious diseases. Garlic contained volatile oil and alisin which containing sulfur. The present study was to evaluate the antibacterial potential of fresh and powder of garlic (Allium sativum L.) extracts against the bacterial pathogens  Salmonella typhi  and describes the phytochemical analysis. Methods: In the present study the antimicrobial potency of fresh and powder of garlic  was carried out by  disc  diffusion and dilution method against Salmonella typhi bacterial and qualitatively tested for the presence of chemical constituents for the identification of flavonoids, saponins, tannins, alkaloids by the method described previously. The results of the present study showed the  fresh and powder of garlic (Allium sativum L.) same as susceptible  for Salmonella typhi. The (minimum inhibitory concentration) MIC of fresh of garlic  at 10% and the powder of garlic  at 8%. The most effective concentration of fresh and powder of garlic  for Salmonella typhi is 90 %.  Phytochemical screening of ethanol extract of Allium sativum L revealed the presence of various bioactive components of flavonoids, saponins, tannins and alkaloids were the most prominent.   Key words : Allium sativum, Antibacterial, Salmonella typhi
UJI ANTIBAKTERI DAN FORMULASI SEDIAAN MASKER ANTI JERAWAT YANG MENGANDUNG KAYU MANIS (Cinnamomum burmanni Nees &T. Nees ) Komala, Oom; Noorlaela, Ella; Dhiasmi, Andhika
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Publisher : Fakultas Matematika dan ilmu pengetahuan Alam. Universitas Pakuan.

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Abstract

Cinnamon contains eugenol and cinnamaldehyde compounds and is widely used as an anti acne. The aim of this research make cinnamon maskers which has antibacterial power of Staphylococus aureus, to know the effect of cinnamon maskers storage to quality, and  know Cinnamomum burmanii maskers prefered  by  panelist. Cinnamon extract is made by maceration method, Test the antibacterial activity of  maskers  by  diffusion  method. The evaluation of   maskers is done   stability test at  room temperature (25oC-30oC) and temperature 40oC for 8 weeks, and  test prefered  by panelists. Cinnamon  extract  maskers  is made with various concentrations of formula 1 with cinnamon extract 0%, formula 2 with 8% concentration, formula 3 with 10% concentration and formula 4 with 12% concentration, and  tetracycline is control. The results of  the study were known  maskers with  cinnamon 12% concentration  most effective as an antibacterial with average of 16 mm diameter and  most preferred, stable at  room  temperature  25oC-30oC  and  40oC for 8 weeks. pH of  Maskers ranges from 5.31 to 6.89 which is still the normal range for pH of the preparation. Key words: Pandan fragrance, Streptococcus mutans, mouthwash
PENGELOLAAN SAMPAH ORGANIK MENGGUNAKAN MIKROORGANISME Komala, Oom; Sugiharti, Dewi; Darda, Rouland Ibnu
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Publisher : Fakultas Matematika dan ilmu pengetahuan Alam. Universitas Pakuan.

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Abstract

The study of organic waste using microorganisms has been carried out, aiming to find the concentration of microorganisms that can deodorize and can degrade organic waste into compost and fertilize the plants. The method used for waste is experimental with 4 treatment is not given microbe, 25 ml microbe concentrations of 106, 50 ml microbe concentrations of 106 and 75 ml microbe concentration of 106 under anaerobic conditions. After 20 days of fermenting compost is given at 1 month age chilli crop at a dose of 15 g per week for 5 weeks. Waste parameter includes aroma, pH, texture and temperature. In chilli crop plant height, number of leaves, fresh weight and dry weight, were analyzed using factorial completely randomized design 4x4 to see the influence of  crops. The results can be seen that organic waste can be managed using a mix of microbes into compost that can improve plant growth chillies with a dose of 25 ml concentrations of 106 of the best.
UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN EKSTRAK AIR KELOPAK BUNGA ROSELLA (HIBISCUS SABDARIFFA L.) TERHADAP BAKTERI STREPTOCOCCUS PNEUMONIAE Komala, Oom; Rosyanti, Reni; Muztabadihardja, Muztabadihardja
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.519

Abstract

The in vitro study of antibacterial effectiveness of Rosella (Hibiscus sabdariffa L.) sheath ethanol extract and water extract on Streptococcus pneumoniae was conducted by diffusion and dilution method. Basic solution amounting to 70g/100 ml of the extract was diluted into 10%, 30%, 50% and 70% for diffusion test with positive control ampicillin 10 IU, and 0,1%, 0,25%, 0,5%, 1%, and 2% ethanol extract for dilution test on S. pneumoniae. The results showed that the ethanol extract and water extract could delay of bacterium growth. Agar diffusion test (disk diffusion) was observed by diameter of bar region around the disk. At concentration 70 % among extract ethanol and water extract made bar zone wide the best, the diameter mean was estimated 25.6 mm for ethanol extract, and 24.3 mm for water extract on S. pneumoniae. In dilution test, the result showed Minimum Inhibitory Concentration (MIC) of the extract was 1% on S. pneumoniae. The result of phytochemicals test is known that ethanol extract and water extract of rosella contains of saponin, tanin, and flavonoid.
Aktivitas Antimikroorganisme Ekstrak Etanol 70 % Biji Bengkuang Terhadap Staphylococcus epidermidis, Pseudomonas aeruginosa dan Candida Albican Min Rahminiwati; Joshie Ramadhan; Oom Komala
Jurnal Sain Veteriner Vol 38, No 3 (2020): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.44589

Abstract

Selain sebagai biopestisida ekstrak etanol 70 % biji bengkuang dilaporkan mempunyai aktivitas antibakteri terhadap bakteri gram positif Staphylococcus aureus dan bakteri gram negatif Escherichia coli dan Candida alibican. namun efeknya terhadap Staphylococcus epidermidis dan Pseudomonas aeruginosa, belum dketahui. Penelitian ini ditujukan untuk menentukan aktivitas antimikroorganisme ekstrak etanol 70 % biji bengkuang lokal  terhadap  Staphylococcus epidermidis, Pseudomonas aeruginosa dan  Candida Albican menggunakan teknik difusi agar dan metoda dilusi.  Aktivitas antimikroorganisme ekstrak diteliti pada konsentrasi 10% 12% 14%, 16% dan sebagai kontrol negatif, kontrol positif antibakteri dan kontrol positif antifungal masing masing digunakan akuadestilata.  ertromisin 10 ppm, ketokonazol 10 ppm. Data yang diperoleh menunjukkan bahwa ekstrak efektif sebagai antibakteri terhadap Staphylococcus epidermidis dan Pseudomonas aeruginosa serta sebagai antifungi terhadap Candida albican, pada konsentrasi bunuh minimum 10 mg/ml. Kata kunci: Ketokonazol, Eritromisin Pseudomonas biji bengkuang Candida albikan
Co-Authors . Ismanto . Yulianita, . Andhika Dhiasmi Asmara, Herra Indra Bina Lohita Sari Bina Lohita Sari Darda, Rouland Ibnu Dewi Sugiharti Dhiasmi, Andhika Dina Agustina Durrotun Nafisah Abd.Nf Dwi Indriati E Mulyati Effendi E. Mulyati Effendi Ella Noorlaela Fatimah Zahra Fuji Raka Siwi Herra Indra Asmara Ike Yulia Ike Yulia Wiendarlina Indriati, Dwi Intan Lestari Joshie Ramadhan Lia Puspita Sari Lubis, M. Akhmar Yusfi M. Akhmar Yusfi Lubis Min Rahminiwati Moerfiah Moerfiah Muhammad Alan Maulana Muztabadihardja Muztabadihardja Muztabadihardja Muztabadihardja Muztabadihardja, Muztabadihardja Muztabadihardja, Muztabadihardja Nina Sakinah Nina Sakinah Noorlaela, Ella Novi Fajar Utami Nur’aini, Putri Pebrianti, Rita Prasetyorini Prasetyorini Putri Putri Dwi Antini Putri Nur’aini Reni Rosyanti Reni Rosyanti Rita Pebrianti Rita Rahmawati Rosyanti, Reni Rosyanti, Reni Saraswati Darmawan Septia Andini Siti Mariyam Rosdiana Sri Suci Damayanti Sri Wardatun Sri Wiedarti Sri Wiedarti Sugiharti, Dewi Tri Saptari Haryani Uswatun Hasanah Utami, Novi Fajar Vira Mourena Putri Widjaya Wiendarlina, Ike Yulia Yulia, Ike Yulianita .