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The Larvacidal Activity of Ethanol Extracts of Phyllanthus acidus Leaves on The Culex quinquefasciatus Instar III/IV Larvae Afifatun Hasanah; Bagus Hermansyah; Cholis Abrori
Journal of Agromedicine and Medical Sciences Vol 5 No 2 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i2.6842

Abstract

Culex quinquefasciatus was one of a vector of filariasis disease caused by filarial worm infection. Mode of transmission was through mosquito bites that contain filaria worm. To overcome the problem in terms of eradicating the mosquito larvae was by searching for safe biological materials for humans and the environment, one of them was Phyllanthus acidus leaves. The purposes of this research were to know whether the ethanol extract of Phyllanthus acidus leaves have larvicidal activity on Culex quinquefasciatus instar III / IV larvae and to know the lethal concentration 50 (LC50) ethanol extract of Phyllanthus acidus leaves on Culex quinquefasciatus instar III / IV larvae within 24 hours. This study used true experimental method with post test only controlled group design with 1 positive control group (Temephos), 1 negative control group (Aquades and DMSO), and 5 treatment groups (0.0625%; 0.125%; 0.25%, 0.5% and 1% extract) respectively 20 Culex quinquefasciatus instar III/IV larvae. The result of regression test of inverse to know the effect of leaf extract of ceremai to the death of Culex quinquefasciatus larvae obtained R2=0909 which was mean the effect of ethanol extract of Phyllanthus acidus leaves to the number of the death of Culex quinquefasciatus larvae equal to 90.9%. The result of probit test obtained LC50 result of 0.189% with 95% confidence interval (0.142% -0.233%).
Association between Personal Hygiene and Incidence of Soil-Transmitted Helminthiasis among Workers at Widodaren Plantation in Jember Regency Zulaikha Rizqina Rahmawati; Bagus Hermansyah; Erfan Efendi; Yunita Armiyanti; Yudha Nurdian; Wiwien Sugih Utami
Journal of Agromedicine and Medical Sciences Vol 6 No 1 (2020)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v6i1.9593

Abstract

Soil-Transmitted Helmithiasis is an infection caused by intestinal nematode worms which in the process of transmission requires soil as media to fullfil its life cycle from non-infective forms to infective forms. The number of infections is influenced by personal hygiene. Personal hygiene consists of the habit of washing hands, cutting nails, eating, defecating and ownership of latrines, and the use of personal protective equipment. This study used a cross sectional research design conducted at Widodaren Plantation with 68 people of samples. Stool examination was determined by kato-katz method, sedimentation, and floatation to detect the presence of Soil-Transmitted Helminth (STH) eggs, while personal hygiene was assessed using questionnaires. Questionnaire and stool examination datas were processed using SPSS data analysis with chi-square method. The results of this study found 26 respondents who were positive for soil-trasmitted helminthiasis infection. The most frequent worm species obtained in this study were Ascaris lumbricoides (57.7%), followed by multiple infections between Hookworm and A. lumbricoides (21.3%), and Hookworm (19.2%). In the chi-square analysis the results were significant between personal hygiene and soil-transmitted helminthiasis with p value of 0,000 (p <0.05). So, it can be concluded that a bad personal hygiene factor has a significant relationship to the incidence of Soil-Transmitted Helminthiasis at Widodaren Plantation. Keywords: Personal hygiene, STH, plantation
Description of Leukocytes Differential Count in Coffee Plantation Workers Silo Subdistrict that Infected by Soil-transmitted Helminths Desi Dwi Cahyani; Yunita Armiyanti; Cicih Komariyah; Bagus Hermansyah; Yudha Nurdian
Journal of Agromedicine and Medical Sciences Vol 6 No 1 (2020)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v6i1.9620

Abstract

Helminthiasis caused by soil transmitted helminths (STH) are still a serious health problem in the world and in Indonesia. Indonesia has a prevalence that varies between 2.5%-62%. This infection can cause blood disorders such as leukocytosis, eosinophilia, and changes in hemoglobin levels. The purpose of this study was to describe the leukocyte count of coffee plantation workers that infected by STH. This research was an observational descriptive study, using a cross sectional design and was conducted at the coffee plantation in Silo subdistrict. Stool examination was conducted by the concentration method (sedimentation and flotation) to determine the presence of STH infection, while for leukocyte count, we used the differential count method. The results of examination on 101 feces samples showed 26.7% (27/101) of workers were positively infected with STH, with details of the STH type of hookworm by 92.6% (25/27), and the remaining were double infections by Ascaris lumbricoides and hookworm as much as 7.4 % (2/27). Examination of leukocyte count showed, 17 workers with hookworm infection had abnormal leukocyte count i.e eosinophilia and neutrophilia, while 8 workers were normal. All workers with double infection (2 workers) had abnormal leukocyte count i.e eosinophilia and neutrophilia. These results can be influenced by the chronicity of the infection or the intensity of the infection. Keywords: Chronic Kidney Disease, sodium, re-use hemodialyzer
THE RELATION OF PERSONAL HYGIENE WITH THE RISK OF OCCURRENCE OF SOIL-TRANSMITTED HELMINTH CO-INFECTION IN TB PATIENTS IN THE WORKING AREA OF THE JENGGAWAH HEALTH CENTER IN JEMBER REGENCY Ellen Ocktavironita; Bagus Hermansyah; Angga Mardro Raharjo; Diana Chusna Mufida; Dini Agustina; Muhammad Ali Shodikin
Majalah Kedokteran Sriwijaya Vol 52, No 2 (2020): Majalah Kedokteran Sriwijaya
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/mks.v52i2.11977

Abstract

Tuberculosis (TB) and soil-transmitted helminth (STH) infections are infectious diseases with a high prevalence in Indonesia. STH co-infection in TB patients can worsen the prognosis of TB disease due to the dysregulation of the immune response. The high prevalence of STH infections in Indonesia is caused by environmental factors such as poor personal hygiene habits. Jenggawah Subdistrict is an area with a high number of TB cases in Jember Regency and most of the area is in the form of agricultural fields and plantations that have a suitable humidity and temperature for the development of STH. The purpose of this study was to determine the relationship of personal hygiene risk factors with the risk of STH co-infection in TB patients in the work area of the Jenggawah Health Center in Jember Regency. This study used an observational analytic design with a cross sectional method and was conducted from September to December 2019. The research was conducted at the TB Polyclinic in Jenggawah Health Center in Jember Regency with a total sample of 26 respondents who were given a personal hygiene questionnaire. Stool examination was conducted at the Laboratory of Parasitology FK UNEJ using sedimentation and flotation methods. The results showed the incidence of STH co-infections was 15.3%, good personal hygiene was 57.7%, and bad personal hygiene was 42.3%. Fisher exact test results showed that there was no significant relationship between personal hygiene risk factors and the risk of the occurrence of STH co-infection in TB patients in the working area of the Jenggawah Health Center in Jember Regency (p = 1,000).
Hubungan perilaku hidup bersih dan sehat dengan kejadian infestasi Soil–transmitted Helminths pada pekerja perkebunan kopi Sumber Wadung Kabupaten Jember Ahmad Hasbi Al-Muzaky; Bagus Hermansyah; Enny Suswati; Yunita Armiyanti; Yudha Nurdian
JURNAL KEDOKTERAN DAN KESEHATAN Vol 6, No 1 (2019)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.379 KB) | DOI: 10.32539/JKK.v6i1.7233

Abstract

Infestasi Soil - Transmitted Helminths (STH) merupakan masalah kesehatan yang masih sering ditemukan di negara beriklim tropis, salah satunya di Indonesia. Hal ini dikarenakan perkembangan STH membutuhkan kondisi lingkungan yang hangat dan lembab, serta  memerlukan media tanah untuk menjadi fase infektif yang disebut soil - transmitted helminth.Kondisi ini dapat ditemukan di lingkungan perkebunan yang sangat menguntungkan bagi perkembangan STH. Hal ini menyebabkan para pekerja perkebunan sangat beresiko terinfestasi oleh STH. Tujuan penelitian ini adalah mengetahui adanya hubungan perilaku hidup bersih dan sehat (PHBS) dengan kejadian infestasi STH pada pekerja perkebunan kopi. Jenis penelitian ini adalah penelitian analisis deskriptif dengan desain cross-sectional yang dilaksanakan pada bulan Desember 2018 hingga Januari 2019. Perkebunan yang dipilih adalah Perkebunan Kopi Sumber Wadung di Kabupaten Jember dengan jumlah sampel sebanyak 100 orang responden dan diberikan kuisioer PHBS. Pemeriksaan infestasi STH dilakukan dengan dua metode yaitu flotasi dan sedimentasi. Dari 100 responden, 28 pekerja (28%) terinfestasi oleh STH. Kemudian dilakukan uji analisis chi-square antara PHBS dengan kejadian infestasi STH dan didapatkan adanya hubungan antara PHBS dengan kejadian infestasi STH pada pekerja (<0,05).
Combination Effect of Methanol Extract of Hibiscus rosasinensis L. Leaf and Ciprofloxacin against Shigella dysentriae In Vitro Billy Jusup Kurniawan; Muhammad Ali Shodikin; Bagus Hermansyah
Journal of Agromedicine and Medical Sciences Vol 4 No 2 (2018)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v4i2.6541

Abstract

One of way to preventing bacterial resistance is a combination of natural plant products with antibiotics. Hibiscus rosasinensis leaf proved to have antibacterial activity against Shigella dysentriae. Ciprofloxacin is the first-choice antibiotic to treat Shigella dysentriae infection. The purpose of this study was to determine the effect of methanol extract of Hibiscus rosasinensis leaves and ciprofloxacin against S. dysentriae bacteria in vitro. The type of this study was quasi experimental design with posttest only control group design using nine treatment groups (with concentration variation of 0.5 μg / mL, 1 μg / mL, 2 μg / mL, 4 μg / mL, 8 μg / mL, 16 μg / mL, 32 μg / mL, 64 μg / mL, and 128 μg / mL with 5 μg / 5 μL ciprofloxacin) and one control group (only 5 μg / 5 μL ciprofloxacin). The method used was agar well diffusion. The data was the diameter of inhibition zone around the well. The results showed that the variant of hibiscus leaf concentration could inhibit the growth of Shigella dysentriae but formed smaller inhibition zone diameter than ciprofloxacin alone. Keywords: combination, hibiscus rosasinensis leaf, ciprofloxacin, Shigella dysentriae
The Difference of Potassium Level in Stage 5 Kidney Chronic Disease Patients Whose Using New and Re-use Hemodialyzer in RSD dr. Soebandi Jember Yuli Hermansyah; Dinda Ayu Wanodya Supriatiningsih; Bagus Hermansyah
Journal of Agromedicine and Medical Sciences Vol 5 No 3 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i3.9540

Abstract

Stage 5 chronic kidney disease (CKD) is a condition where the renal function decrease, marked by the GFR value < 15/ml/minute/1,73 m2 with or without kidney damage history for 3 months or more that needs kidney replacement therapy, including hemodialysis. In Indonesia, hemodialysis was chosen for 82% from all the cases that needs kidney replacement therapy. However, the cost for hemodialysis therapy is considered as too expensive and burdens The National Health Insurance, Badan Penyelenggara Jaminan Sosial (BPJS), allowing the reuse of hemodialyzer as an alternative for cost-effectiveness. Re-use hemodialyzer is a term for using the same hemodialyzer for the same patient but on different therapy session. The main purpose of this research is to investigate the difference of Potassium level in stage 5 chronic kidney disease patients whose using new and re-use hemodialyzer in RSD dr. Soebandi Jember. This research used quasi experimental design by using blood sample that will be measured for the potassium level after using new hemodialyzer and re-use hemodialyzer for the 4th time in Hemodialysis Installation of RSD dr. Soebandi Jember on December 2018. Total sample of 19 patients chosen using inclusion and exclusion criteria. Collected data were analyzed using paired t-test. The result of statistical test shows that there is no significance potassium level difference in stage 5 chronic kidney disease whose using new and re-use hemodialyzer for the 4th time (p=0,094). The effectivity of hemodialyzer that still has a good condition and hemodialysis therapy that has been done in accordance with the procedure until the 4th reuse is the main factor of this result. Keywords: CKD, potassium, re-use hemodialyzer
Histopathological Changes in the Wistar Rats Liver Treated with Bangle (Zingiber cassumunar Roxb.) Rhizome Ethanol Extract during Acute Toxicity Test Kesy Sasta Handani; Wiwien Sugih Utami; Bagus Hermansyah; Rena Normasari
Journal of Agromedicine and Medical Sciences Vol 4 No 1 (2018)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v4i1.6327

Abstract

Malaria is still an infectious disease that can not be eradicated. The presence of antimalarial drug resistance case encourages the research on herbal plants that are expected to be a complementary therapy drug of malaria, one of them is bangle (Zingiber cassumunar Roxb.). This research aimed to investigate the effect of bangle (Zingiber cassumunar Roxb.) rhizome ethanol extract to the histopathological changes of Wistar rats liver during acute toxicity test. This research type was true experimental laboratories with posttest only control group design using four treatment groups (given dose 42,609 mg/200gBW + Tween 1%, 213,045 mg/200gBW + Tween 1%, 426,09 mg/200gBW + Tween 1%, and 852.18 mg/200gBW + Tween 1%) and one control group (given 1% Tween). Each treatment groups consisted of five male rats and five female rats. The final mean score of hepatocyte damage as the research data was analyzed by One-Way ANOVA test and showed that p=0,084. It indicated that there was no significant difference of liver histopathology results between groups. Keywords: malaria, bangle rhizome, acute toxicity test, liver histopathology
The Correlation Between The Use of Personal Protective Equipment (PPE) and Soil-Transmitted Helminths Infection in the Workers of Kaliputih Plantation Jember Regency Ivan Iqbal Baidowi; Yunita Armiyanti; Zahrah Febianti; Yudha Nurdian; Bagus Hermansyah
Journal of Agromedicine and Medical Sciences Vol 5 No 2 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i2.9625

Abstract

This study aimed to investigate the correlation between the use of Personal Protective Equipment (PPE) and the status of Soil-Transmitted Helminths infection in the workers of Kaliputih plantation in Jember Regency. The research method applied was Analytic observational with a Cross-sectional approach. The population, as well as the sample, were all workers of Kaliputih Plantation, Sumber Bulus Village, Ledokombo District, Jember Regency, by the total of 63 people. The sampling technique was the Total sampling. Laboratory analysis on faecal samples of respondents were conducted at the Parasitology Laboratory, Faculty of Medicine, University of Jember. Furthermore, the research data were analyzed using Chi-Square analysis or Fisher’s Exact Test. The results demonstrated that the prevalence of STH infection in Kaliputih Plantation workers was 25%. This figure was supported by the good awareness of the use of Personal Protective Equipment (PPE) of workers (91.67%). In addition, the Soil-Transmitted Helminths species that infested the plantation workers were Ascaris lumbricoides, found in 6 people (16.67%), and Hookworm, found in 3 people (8.33%). Finally, the result of Chi-square analysis showed a significance value of <0.05. Therefore, it can be concluded that the use of PPE in Kaliputih plantation workers had a significant correlation with the STH infection status. Keywords: Correlation, PPE, Soil-Transmitted Helminths Infection