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Multiple Antibiotic Resistance Index of Escherichia coli Isolates from Dumai Sea Waters Riau Province Mardalisa Mardalisa; Feliatra Feliatra; Nursyirwani Nursyirwani
Berkala Perikanan Terubuk Vol 49, No 1 (2021): Februari 2021
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/terubuk.49.1.734-739

Abstract

The pattern of antibiotic consumption by the community which is carried out independently without medical guidance causes antibiotic pollution has reached sea waters, one of its impacts on the nature of bacterial resistance. This study aims to look at the pattern of antibiotic sensitivity to E coli that isolated from Dumai sea waters. E coli isolates that grown on MHA media were tested based on the Kirby Bauer disk diffusion method. Seventeen isolates were identified, among which a high rate of resistance to penicillin, isoniazid and streptomycin (17), a high rate of resistance to chloramphenicol (5), intermediate susceptibility to chloramphenicol (11) and only 1 sensitive to chloramphenicol. The multiple antibiotic resistance (MAR) index of E coli isolates was 0.82. These show that antibiotics like penicillin, isoniazid, streptomycin and chloramphenicol were not suitable antibiotic for E coli infection treatment. The community is expected to be more disciplined in using antibiotics and to be careful in using the Dumai sea for fishery activities
CHARACTERIZATION OF LACTIC ACID BACTERIA FROM CINCALUK BASEDON16S rRNA SEQUENCE Putri Ayu; Nursyirwani Nursyirwani; Feliatra Feliatra
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT Lactic acid bacteria (LAB) is a groupof bacteria frequently used as probiotic in aquaculture because of its beneficial effects on health, pathogenic bacteria prevention and increase of fish growth. LAB could be found in fermented fish products such as Cincaluk that is produced by people in Tembilahan and Bengkalis of Riau Province. The purpose of this research was to characterize LAB isolated from Cincaluk Tembilahan and Bengkalis genetically. This research was conducted from February until May 2018. Bacterial isolates were identified based on morphology, biochemical characters and 16S rRNA sequence. 24 isolates found were round, creamy white, Gram positive, did not produce indole, produced catalase, non motil, used citrate, did Methyl Red, produced H2S and gas, glucose, sucrose and lactose were fermented. Based on 16S rRNA sequence. Five of six isolates selected were identified as Lactobacillus plantarum (97-100% similarites) and one isolate was Bacillus cereus (94%) Keyword:Characteristic, Lactid Acid Bacteria, Cincaluk, Genetic,16S rRNA sequence 
ISOLATION OF OIL DEGRADATION BACTERIA FROM SEDIMENT IN SUNGAI PAKNING WATERS OF BENGKALIS REGENCY AND THE ABILITY TO DEGRADE CRUDE OIL Gina Ulfa Fitria; Nursyirwani Nursyirwani; Thamrin Thamrin
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT Oil pollution in Bengkalis waters usually comes from land, marine transportation, defection port activities and oil industry. Oil pollution can be trapped in sediment because it can not be dissolved and difficult to degrade, one way to overcome pollution is by bioremediation. The purpose of this research was to isolate bacteria from sediment Sungai Pakning waters, to examine the ability of isolates in degrading crude oil and to identify the bacterial isolates genetically based on sequence 16S rRNA. The research was conducted from March to May 2018. Bacterial isolation was conducted in Laboratory of Marine Microbiology and the oil degradation analysis was conducted in Laboratory of Chemical Oceanography, Faculty of Fishery and Marine Science, University of Riau. This research used survey and experiment method, survey method to measuring of water quality, removal of sample and isolated of bacteria, experiment method to examine the ability of isolates in degrading crude oil. The result showed that eight isolates (7D15, 7D35, 7D23, 14D15, 14D34, 21D13, 21D23, 21D35) were able to degrade crude oil at 1-3% concentrations. The highest degradation at 1% concentration was indicated by 21D35 isolate, at 2%  concentration is indicated by  21D13 isolate, and at 3% concentration is indicated by 7D23 isolate. To molecular identification results by the 16S rRNA sequence take of three isolates have the highest degradation ability. 21D13 isolate was similar to Achromobacter pulmonis, 21D35 isolate was similar to Achromobacter sp., and 7D23 isolate was similar to Vibrio sp. Keyword : Pakning River, Oil Degradation Bacteria, 16 rRNA, Achromobacter, Vibrio
ACTIVITY OF HETEROTROPHIC BACTERIA ISOLATED FROM MASJID RIVER, DUMAI, AGAINST PATHOGENIC BACTERIA Zhilalul Huda; Feliatra Feliatra; Nursyirwani Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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                                                ABSTRACT Heterotrophic bacteria is a group of organism that play important role in biogeochemical processes in aquatic ecosystem.The bacteria are able to decompose and remineralize organic materials into a simple inorganic components which is turn to nutrients for phytoplankton, perifiton, and other aquatic microflora. This Study was conducted from September to October 2018. The purpose of this study wos to obtain bacterial isolates that can be used as an antibacterial against pathogenic bacteria and to identify bacteria found in River estuary Masjid Dumai of Riau Province. Based DNA character the result found10 bacterial isolates. The bacterial (1A, 1B, 1C, 1D, 1E, 1F,1J, 2C, 2D, and 2G). These bacteria were able to inhibit the pathogenic growth. DNA identification using PCR and 16S rRN sequence technique resulting found6 types of bacteria, namely Bacillus cereus strain ATCC 14579, B. anthracis strain ATCC 14578 16S,B. methylotrophicus strain CBMB205 16S, B. thuringensis strain YBT-1518, B. pseudomycoides strainNBRC, and B. anthracis strain C10.Keywords: Heterotrophic Bacteria, Antibacteria, 16S rRNA Sequence, Pathogens.
ANALYSIS OF Pb, Cu, and Zn METAL CONNECTIONS IN BLOOD SHELL MEAT (Anadara granosa) WITH DIFFERENT SIZES IN WATERS MUARA ASAHAN RIVER TANJUNGBALAI CITY NORTH SUMATERA Syafri Syafri; Bintal Amin; Nursyirwani Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT  granosa.This research was conducted from January to April 2019 by taking samples of blood cockles from the waters of the Asahan river, Tanjungbalai city, North Sumatra Province. The purpuse of this research were to know the metal concentrations of Pb, Cu and Zn in different station and different sizes of blood cockle’s shell (Anadara granosa), as well as to find out the level of metal pollution based on MPI (Metal Pollution Index). Analysis of metal concentrations of heavy metal was done by following procedurs outline by Yap et al., (2003). The results showed that the highest Pb metal concentration in meat of small blood clams was found at station 3 namely, 0.7100 μg / g and the low concentration was 0.4716 μg / g at Station 1. While the highest concentration at medium size was found at Station 2 namely , 1.3683 μg / g and low concentration was 0.8000 μg / g at Station 1. The highest Pb concentration at large size was found at Station 3 ie, 2,2200 μg / g and the lowest concentration was 1,2266 μg / g at Station 1. The calculated MPI value for the Asahan estuary research from this study was 3,7260  which was considered quite low compared to other regions. Keywords: Heavy Metal, Pollution, North Sumatra, Industry, Anadara
THE CONDITION OF CORAL REEFS IN THE WATERS OF TIDUNG ISLAND KEPULAUAN SERIBU JAKARTA Muhammad Hafiz Perdana; Thamrin Thamrin; Nursyirwani Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT The aims of this study are to determine the condition of the coral reefs including percentage live coral cover, the diversity index, the uniformity index, and the dominance index of  lifeform coral at waters of Tidung Island Kepulauan Seribu Jakarta. This research has been conducting from 2018 May 7th - 17th at the waters of Tidung Island.The research station was divided into 3 stations based on environmental characteristics with 2 different depths ; 3 and 7 meters. The research station is a coral conservation area, snorkeling area, and natural area. Data of coral reef was obtained by using the Line Intercept Transect (LIT) method. The results showed that the conditions of coral reefs at stations 1 and 2 respectively in the bad category with the percentage of cover area are 3.52% and 10.84%. Whereas at station 3 the condition of coral reefs is in the medium category with the percentage of cover area is 30.01%. The low percentage of coral cover is caused by rising sea water temperatures, port activities and human activities. Water quality such as temperature, salinity, pH, current velocity and brightness at the study site is quite good for coral recovery and growth. Keyword : LIT, Coral Recovery, Diversity, Uniformity, and Dominance Index
ISOLATION OF DIESEL OIL DEGRADATION BACTERIA IN THE WATERS AROUND THE PORT OF TELUK BAYUR PADANG CITY Sri Melinda Citra; Dessy Yoswaty; Nursyirwani Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract

ABSTRACT Diesel oil degradation bacteria can be found in various habitats in the marine environment including in the waters around the harbor. This research aims at diesel oil in the waters around the port of Teluk Bayur and test its ability to degrade diesel oil. Isolation of the degrading bacteria has been from April to conductedJune 2018 in the Marine Microbiology Laboratory, Department of Marine Science, Faculty of Fishery and Marine University of Riau.Total count of diesel oil decomposing bacteria ranges from 0.4 x 106 to 7.3 x 106 CFU/ml. Eleven isolates of the diesel oil degradation bacteria were selected, ie BM1a, BM1b, BM1c, BM1d, BM7a, BM7b, BM7c, BM7d, BM14a, BM14c and BM14d. Based on morphological, physiology and biochemical characteristics, seven isolates were irregular, two round and concentric isolates, seven isolates with corrugated colony edges, two irregular and notched isolates, six yellow colony isolates, five white isolates, colony elevation all isolates emerge, all isolates were positive on catalase test, in H2S test all negative isolates, all isolates in the sugar test produced gas, all isolates were motile and Gram negative, all indole isolates were negative, seven methyl red positive isolates and four methy red isolates negative. In the degradation test only six bacterial isolates were selected, ie BM1a, BM1c, BM7a, BM7d, BM14c and BM14d. The highest degradation of diesel oil at concentrations 1%, 2% and 3% were performed by isolates BM1c, BM7a and BM1a respectively. Overall, all isolates were able to degrade diesel oil in the waters around Teluk Bayur Port, Padang City. Keyword: Isolation, Bacteria, Degradation, Diesel Oil 
DENSITY OF Escherichia coli BACTERIA AND HETEROTROPHIC BACTERIA IN DUMAI SEAWATER OF RIAUPROVINCE Anisa Qoriman; Feliatra Feliatra; Nursyirwani Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT This research was conducted from April until May 2018. The purpose of this study was to calculate the density of Escherichia coli in sea water of Dumai, to determine the ratio of E. coli to heterotrophic bacteria and to examine the resistance of E. coli to antibiotics. In this study the determination of sampling points using purposive sampling method, was set intothree sampling points for each station. Density ofE.coliwas calculated by using the Most Probable Number (MPN) method and density total of heterotrophic bacteria by using spread plate count method. The result showed that densityE. colirangedfrom 2.37 x 104 – 1.1 x 105 CFU/ml. The highest MPN number ofE. coliwas found in stations 2 and 3, andthe lowest wasin station 5. The total number of heterotrophic bacteria ranged from 7.37 x 106 – 10.38 x 106 CFU/ml. The highest number of heterotrophic was foundin station 4 and the lowest was in station 5.The highest percentage ratio of density of E.colito heterotrophic bacteriawas in station 2(1.20%)and the lowest was in station 5 (0.32%).Resistance of E.coli toward chloramphenicol as antibiotic testedindicated that 7 isolateswere resistant with the inhibition zone ranged from 2.17 –10.70 mm, 9 isolates were categorized intointermediate with the inhibition zone ranged from 11.90–19.33 mm and 1 isolate was sensitive with the inhibition zone was 22.77 mm. Towardantibiotics penicillin andisoniazid, all bacterial isolates were resistant with the inhibition zone ranged from 0.80 - 4.70 mm and 1.50- 4.67 mm, respectively.Keyword: Density, Escherichia coli,MPN method, Heterotrophic Bacteria, Antibiotics 
ANALISIS DAMPAK LINGKUNGAN BUDIDAYA IKAN DALAM KERAMBA DI WADUK SUNGAI PAKU KECAMATAN KAMPAR KIRI KABUPATEN KAMPAR Yulhadis Yulhadis; Usman M. Tang; Nursyirwani Nursyirwani
Jurnal Ilmu Lingkungan Vol 12, No 1 (2018): Jurnal Ilmu Lingkungan
Publisher : Program Pascasarjana Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jil.12.1.p.15-26

Abstract

The study was conducted in January-March 2018, field analysis (insitu) was conducted in Sungai Paku Reservoir of Sungai Paku Village Kampar Kiri Sub-district Kampar District Riau Province. Laboratory analysis (exsitu) was conducted in Ecology and Waters Management Faculty of Fishery and Marine Science University of Riau Pekanbaru. The purpose of this research is to analyze the quality of Sungai Paku reservoir to the floating net cage (FNC) activity, to analyze and develop the socialization of FNC management in the Sungai Paku reservoir. This research uses survey method, by conducting direct measurement in the field, conducting analysis and interviews of research location through questionnaire to get more accurate data. To analyze the environment at about 3 different stations over the inlet of the reservoir, within the FNC area, and outlet of the reservoir. Physical chemistry parameters include 95-95.2 cm Brightness, temperature 30-30.3oC, current velocity 3.92 - 3.97, dissolved oxygen 0.98 - 1.00 mg / l, pH 5, Phosphate 0.1850-0.1851 mg / L, nitrate 0.2102-0.2104 mg / l , ammonia 0.0675-0.0677 mg / l. The average abundance of phytoplankton 17,600 - 22,400 cells / ml. Abundance of macrozoobenthos 866 - 1471 Ind / m2. Evaluation of water quality at each observation station indicates that the recording at each station is DO, pH and total P which have not meet the standard of water quality.
ANTAGONISM HETEROTROPHIC BACTERIA ISOLATED FROM ESTUARY OF SIAK RIVER AND SETTLED SEA OF SIAK REGENCY AGAINST PATHOGENIC BACTERIA Andrei Putra Zirma; Feliatra Feliatra; Nursyirwani Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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   Heterotrophic bacteria is bacteria that use organic substances as its nutrition sources. Organic substances are obtained from the rest of other organisms, waste or substances contained in the body of other organisms. This study aims to determine the activity of heterotrophic bacteria against pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas sp.). Total amount of bacteria at station 1 was higher than station 2, those were 2.3 x 107 cfu / ml at station 1 and 1.8 x 107 cfu / ml at station 2. Statistical analysis using the simple linear regresion indicated that the correlation between the growth of heterotrophic bacteria on environmental parameters (DO, pH, temperature and salinity) was very weak of wich correlation value(r) was 0.20  for all parameters. Antagonism test against pathogenic bacteria found that there were 8 bacterial isolates wich potentially inhibited the growth of the three pathogenic bacteria, the isolates were A11, A12, A14, A19, A21, A22, A23 and A25 with the inhibition zone ranges 4,8 - 14,5 mm. From the DNA sequences with 16S rDNA method and BLAST analysis, the bacteria isolates were identified asKerstersia gyiorum, Kerstersia sp.,Enterococcus sp. and three isolates was unidentified possibly due to these species hadnot registered in GenBank or had never been identified beforeKey words: Heterotrophic Bacteria, Antagonism, Estuary, 16s rDNA