Article Per Year (5 Year)
p-Index From 2019 - 2024
0.983
P-Index
This Author published in this journals
All Journal Buletin Peternakan Jurnal Veteriner Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Matematika Sains dan Teknologi Jurnal Kedokteran Hewan
Rony Marsyal Kunda
Department Of Biology, Faculty Of Mathematics And Natural Science, Universitas Pattimura, Ambon, 97233, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Veterinary Other

Published : 9 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 9 Documents
Search

 1 
IDENTIFIKASI JENIS-JENIS TUMBUHAN SEBAGAI PAKAN KUSKUS (Phalangeridae) ASAL MALUKU DI TAMAN NASIONAL MANUSELA BAGIAN UTARA KABUPATEN MALUKU TENGAH Handayani, Sri Kurniati; Kunda, Rony Marsyal
Jurnal Matematika Sains dan Teknologi Vol 20 No 1 (2019)
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/jmst.v20i1.814.2019

Abstract

Cuscus is a marsupials, nocturnal, prehensile tails, and included to the family of Phalangeridae. In Indonesia, Ailurops, Phalanger, Spilocuscus and Strigocuscus have limited distribution on Eastern Indonesia (Sulawesi, Maluku, Papua and Timor Island). In Indonesia cuscus are protected animals. This is due to the decline in population in its natural habitat due to deforestation, poaching and illegal wild life trade. Study is needed on the type of cuscus feed in native habitat base on the original character of vegetation in the hope of helping wild life conservation efforts in the future. The strategy of cuscus conservationin-situ and ex-situfrom native habitat will be more focused and effective if the potential information of plants as a food source can be known with certainty.The purpose of this study was to determine the types of plants as feed cuscus (Phalangeridae) from Maluku in the Northern part of Manusela National Park, Central Maluku district. The results showed 44 species of forest plants consumed by cuscus in Manusela National Park. The cuscus from Maluku consume young leaves from 29 species plants  (66%), fruit from 26 species plants (59%), flowers from 4 species plants (9%), shoots from 3 species (7%) of plants. Eight plant species (18%) are consumed its fruit and young leaves, two plant species (5%) are consumed its fruit and flowers, one plant species (2%), consumed its fruit and shoots, and two plant species ( 5%) are consumed its young leaves, flowers, and fruit. Palatability of the cuscus showed that each cuscus choos 43 species of plants (98%) as a food, P. orientalis and P.Urinusdid not choose Syzygium aqueum while S. maculatus, and P. vestitus did not choose Flacourtia inemis as resources feed. Kuskus adalah hewan berkantung (marsupial), aktif di malam hari (nocturnal), berekor panjang yang kuat (prehensile), dan masuk dalam famili Phalangeridae. Di Indonesia, kuskus dari anggota genus Ailurops, Phalanger, Spilocuscus, dan Strigocuscus menyebar terbatas hanya di Indonesia Bagian Timur (Sulawesi, Maluku, Papua, dan Pulau Timor). Di Indonesia, kukus termasuk dalam hewan yang dilindungi. Dibutuhkan studi untuk mengidentifikasi jenis pakan kuskus pada habitat alami yang diharapkan dapat membantu usaha konservasi satwa ini di masa mendatang. Strategi konservasi kuskus secara in-situ maupun ex-situ berdasarkan asal habitat akan lebih terarah dan berhasil guna apabila informasi potensi tumbuhan sebagai sumber pakan dapat diketahui dengan pasti. Tujuan penelitian ini adalah untuk mengetahui jenis-jenis tumbuhan sebagai pakan kuskus (Phalangeridae) asal Maluku di Taman Nasional Manusela bagian Utara, Kabupaten Maluku Tengah. Hasil penelitian menunjukkan, ditemukan 44 spesies tumbuhan hutan yang dikonsumsi kuskus di Taman Nasional Manusela. Dari hasil penelitian terlihat bahwa kuskus asal Maluku pada umumnya mengonsumsi bagian tumbuhan yang masih muda berupa daun muda 29 spesies (66%), buah 26 spesies (59%), bunga 4 spesies (9%), dan tunas 3 spesies (6%). Delapan spesies tumbuhan (18%) dengan komposisi buah dan daun muda paling disukai kuskus, dua spesies tumbuhan (5%) dengan komposisi buah dan bunga, satu jenis tumbuhan (2%), dengan komposisi buah dan tunas muda, serta dua spesies tumbuhan (5%) dengan komposisi daun muda, bunga, dan buah. Palatabilitas pada kuskus menunjukkan bahwa setiap genus kuskus memilih 43 spesies tumbuhan (98%) sebagai sumber pakan, P.orientalis dan P. Urinus tidak memilih Syzygium aqueum sedangkan S. maculatus, dan P. vestitus tidak memilih Flacourtia inemis sebagai sumber pakan.
IDENTIFIKASI JENIS-JENIS TUMBUHAN SEBAGAI PAKAN KUSKUS (Phalangeridae) ASAL MALUKU DI TAMAN NASIONAL MANUSELA BAGIAN UTARA KABUPATEN MALUKU TENGAH Sri Kurniati Handayani; Rony Marsyal Kunda
Jurnal Matematika Sains dan Teknologi Vol. 20 No. 1 (2019)
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463.985 KB) | DOI: 10.33830/jmst.v20i1.82.2019

Abstract

Cuscus is a marsupials, nocturnal, prehensile tails, and included to the family of Phalangeridae. In Indonesia, Ailurops, Phalanger, Spilocuscus and Strigocuscus have limited distribution on Eastern Indonesia (Sulawesi, Maluku, Papua and Timor Island). In Indonesia cuscus are protected animals. This is due to the decline in population in its natural habitat due to deforestation, poaching and illegal wild life trade. Study is needed on the type of cuscus feed in native habitat base on the original character of vegetation in the hope of helping wild life conservation efforts in the future. The strategy of cuscus conservationin-situ and ex-situfrom native habitat will be more focused and effective if the potential information of plants as a food source can be known with certainty.The purpose of this study was to determine the types of plants as feed cuscus (Phalangeridae) from Maluku in the Northern part of Manusela National Park, Central Maluku district. The results showed 44 species of forest plants consumed by cuscus in Manusela National Park. The cuscus from Maluku consume young leaves from 29 species plants (66%), fruit from 26 species plants (59%), flowers from 4 species plants (9%), shoots from 3 species (7%) of plants. Eight plant species (18%) are consumed its fruit and young leaves, two plant species (5%) are consumed its fruit and flowers, one plant species (2%), consumed its fruit and shoots, and two plant species ( 5%) are consumed its young leaves, flowers, and fruit. Palatability of the cuscus showed that each cuscus choos 43 species of plants (98%) as a food, P. orientalis and P.Urinusdid not choose Syzygium aqueum while S. maculatus, and P. vestitus did not choose Flacourtia inemis as resources feed. Kuskus adalah hewan berkantung (marsupial), aktif di malam hari (nocturnal), berekor panjang yang kuat (prehensile), dan masuk dalam famili Phalangeridae. Di Indonesia, kuskus dari anggota genus Ailurops, Phalanger, Spilocuscus, dan Strigocuscus menyebar terbatas hanya di Indonesia Bagian Timur (Sulawesi, Maluku, Papua, dan Pulau Timor). Di Indonesia, kukus termasuk dalam hewan yang dilindungi. Dibutuhkan studi untuk mengidentifikasi jenis pakan kuskus pada habitat alami yang diharapkan dapat membantu usaha konservasi satwa ini di masa mendatang. Strategi konservasi kuskus secara in-situ maupun ex-situ berdasarkan asal habitat akan lebih terarah dan berhasil guna apabila informasi potensi tumbuhan sebagai sumber pakan dapat diketahui dengan pasti. Tujuan penelitian ini adalah untuk mengetahui jenis-jenis tumbuhan sebagai pakan kuskus (Phalangeridae) asal Maluku di Taman Nasional Manusela bagian Utara, Kabupaten Maluku Tengah. Hasil penelitian menunjukkan, ditemukan 44 spesies tumbuhan hutan yang dikonsumsi kuskus di Taman Nasional Manusela. Dari hasil penelitian terlihat bahwa kuskus asal Maluku pada umumnya mengonsumsi bagian tumbuhan yang masih muda berupa daun muda 29 spesies (66%), buah 26 spesies (59%), bunga 4 spesies (9%), dan tunas 3 spesies (6%). Delapan spesies tumbuhan (18%) dengan komposisi buah dan daun muda paling disukai kuskus, dua spesies tumbuhan (5%) dengan komposisi buah dan bunga, satu jenis tumbuhan (2%), dengan komposisi buah dan tunas muda, serta dua spesies tumbuhan (5%) dengan komposisi daun muda, bunga, dan buah. Palatabilitas pada kuskus menunjukkan bahwa setiap genus kuskus memilih 43 spesies tumbuhan (98%) sebagai sumber pakan, P.orientalis dan P. Urinus tidak memilih Syzygium aqueum sedangkan S. maculatus, dan P. vestitus tidak memilih Flacourtia inemis sebagai sumber pakan.
Keragaman Genotip dan Jarak Genetik Sapi Madura Berdasarkan Restriction Fragment Length Polymorphism-DNA (RFLP-DNA) Marleny Leasa; Rony Marsyal Kunda
Buletin Peternakan Vol 36, No 2 (2012): Buletin Peternakan Vol. 36 (2) Juni 2012
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v36i2.1582

Abstract

Penelitian ini bertujuan untuk mengetahui variasi genotip dan jarak genetik sapi Madura di Kabupaten Sampang dan Balai Besar Inseminasi Buatan (BBIB) Singosari berdasarkan teknik RFLP. Digesti DNA genom dengan enzim EcoRI dan PstI menghasilkan fragmen DNA dengan ukuran yang bervariasi baik pada induk, pedet, dan pejantan unggul dengan kisaran antara 10000 bp sampai 980 bp dan 10000 bp sampai 1250 bp. Analisis MVSP1 dengan metode UPGMA untuk jarak genetik ditemukan bahwa sampel sapi Madura berada dalam 2 cluster dan 1 outgroup. Persentase jarak genetik berada pada rentangan 0 sampai 25%. (Kata kunci: Variasi genotip, Jarak genetik, Sapi Madura, RFLP)
Polymorphism of Growth Hormone (GH) Gene in Lakor Goat from Lakor Island of Southwest Maluku Regency Rony Marsyal Kunda; Slamet Diah Volkandari; Maman Rumanta; Pieter Kakisina
Buletin Peternakan Vol 44, No 4 (2020): BULETIN PETERNAKAN VOL. 44 (4) NOVEMBER 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i4.58934

Abstract

Lakor goat survive in Lakor island in Southwest Maluku with high temperature and limited water. Growth trait in goat is interest to explore cause related with economic trait that encoded by growth hormone (GH) gene. The aim of this study was identify of polymorphism GH gene of Lakor goat in Lakor island. A total of 63 samples were collected from three locations (village) i.e Ketti Letpey (18), Werwawan-Yamluli (26), and Letoda (19). DNA was extracted from hair follicles. A 422 bp specific DNA fragment was successfully amplified and genotyped by PCR-RFLP method using HaeIII enzyme. Results showed that polymorphism was found with two variant of genotypes (AA and AB) and two alleles (A and B). AB genotype was dominant in all of populations (93.7%) with A and B alleles were 0.53 and 0.47, respectively. Heterozygosity observed and expected value reached 0.502 and 0.498, respectively while Polymorphic Information Content was in moderate values (0.374). All of populations were in disequilibrium genetic. It maybe caused limited buck and nonrandom mating in population that effect of low genetic variation. Inbreeding study are needed to explore it. The introgression of bucks from other families in several locations within Lakor island can be an alternative solution to increase the genetic diversity of the lakor goat population.
Identifikasi Keragaman Genetik Gen 12S Ribomsom RNA Sebagai Penanda Genetik untuk Penentuan Spesies Kuskus (IDENTIFICATION OF GENETIC DIVERSITY 12SRRNA GENES AS GENETIC MARKER FOR DETERMINING SPECIES CUSCUS) Rini Widayanti; Hery Wijayanto; Woro Danur Wendo; Rony Marsyal Kunda
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.9 KB)

Abstract

Cuscus is marsupial’s animal (Phalageridae) which has limited spread in eastern Indonesia (Sulawesi,Maluku, Papua), Australia and Papua New Guinea. The ex-situ and in-situ conservation of cuscus undercaptivating condition is an alternative solution to protect cuscus from extinction. This study aimed todetermine nucleotide sequence and genetic markers on 12Sr RNA gene with sequencing method of eachspecies on three islands. Whole genome DNA was extracted from 17 samples of cuscus obtained fromdifferent habitats, Sulawesi (2 individual), Maluku (7 individual), and Papua (8 individual) according tothe protocol of Qiamp DNA Blood Mini Kit (Qiagen), and then it was used as template for amplificationof 12Sr RNA gene by using PCR. PCR product were then purified using column chromatography and wereused as template for sequencing reaction. Result sequencing of 12Sr RNA gene were analyzed usingMEGA program version 6. PCR product gives a result nucleotida of 958 bp according to databasegenebank, sequencing product gives result nucleotida of 896 bp and found of 105 different nucleotide sites.Filogram based on nucleotide sequences 12SrRNA gene from Sulawesi cuscus is Ailurops ursinus whereasthe cuscus from Papua and Maluku is Phalanger sp. and Spilocuscus maculatus species. Thirteen nucleotidasites were found, sites no 67 (A/G), 89 (G/C), 137 (T/C), 285 (G/A), 468 (T/C), 595 (T/C, 598 (T/C), 647 (T/C),654 (G/A), 665 (T/C), 769 (C/T), 874 (C/T), and 876 (A/G) which can be used as genetic marker betweenPhalanger genera from Papua and Maluku, and three nucleotida sites (sites no 127 (G/A), 481 (C/T), and885 (T/C) can be used as genetic marker between Spilocuscus genera from Papua and Maluku.
IDENTIFICATION OF GENETIC DIVERSITY CYTOCHROME OXIDASE SUBUNIT II (COII) MITOCHONDRIAL GENE AS GENETIC MARKER FOR ANISAKIS SPECIES IN Euthynnus affinis Prasetyarti Utami; Niken Satuti Nur Handayani; Rony Marsyal Kunda
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (665.25 KB) | DOI: 10.21157/j.ked.hewan.v11i3.2960

Abstract

ABSTRACT This study aimed to get specific genetic marker for Anisakis sp. identification on mackerel tuna using gene sequence cytochrome oxidase subunit II (COII) mitochondrial deoxyribonucleic acid (mtDNA) and to identify taxonomic affiliation between Anisakis sp. from Indonesia and others Anisakis sp. from GenBank database. This study started with sample collections at three fish auctions in Cilacap (Central Java), morphology classification, DNA isolation, and molecular based identification using polymerase chain reaction (PCR) and sequencing methods. Molecular based identification of Anisakis used gene amplification COII mtDNA as a cell target prior to sequence. Morphology characteristic results showed that Anisakis nematodes which infected mackerel tuna classified as type II L3 larvae. Molecular based identification showed significant result, which found 530 bp COII DNA gene fragment similar to target cell. Gene sequencing alignment results of COII Anisakis gene compared with GenBank showed 11 different nucleotide sites that can be used as genetic barcode for Indonesian Anisakis sp. This study showed that Anisakis sp. infected mackerel tuna in Java Sea is Anisakis physeteris and considered as zoonosis.
Study of Biomass in Two Mangrove Ecosystems Maman Rumanta; Sandra Sukmaning Aji; Rony Marsyal Kunda; Frenly Marvi Selano; Ucu Rahayu
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 5 (2023): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i5.3755

Abstract

This study aims to determine the storage of biomass in the mangrove ecosystem of Jakarta Bay compared to Ambon Bay. This research used descriptive quantitative method. Sampling technique using the Point Center Quarter Method (PCQM) which was measured around the chest height of mangrove trees (DBH). Data analysis was performed using an allometric equation where each mangrove plant has a specific gravity. The results of this study indicated that the composition of mangrove species in Ambon Bay consists of 6 mangrove species, namely: Bruguiera cylindrica (L.) Bl, Rhizophora apiculata Bl, Sonneratia alba Smith, Aegiceras corniculatum (L.) Blanco, Avicennia officinalis L, Ceriops tagal. In the ecosystem, Rhizophora apiculata Bl and Sonneratia alba Smith had higher biomass values compared to the other four species. Meanwhile, in Jakarta Bay, the composition of mangrove plants is dominated by Sonneratia alba Smith. The subsurface biomass in the Jakarta Bay and Ambon region is lower than the aboveground biomass.
Impact of Environmental Geographic toward Point Mutations in Exon 1 of Growth Differentiation Factor (GDF9) Gene in Kosta and Lakor Goat Breeds Maman Rumanta; Rony Marsyal Kunda; Slamet Diah Volkandari; Ivan Mambaul Munir
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 6 (2023): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i6.3794

Abstract

This study aims to analyze the impact of environmental biogeography on point mutations in Kosta and Lakor goat breeds as local Indonesian germplasm using the GDF9 Exon 1 gene sequence. A total 43 folicle samples of Kosta and Lakor goats which collected in Serang regency and Lakor island were analyzed. To isolate the total genome of the samples, the tissue isolation method was used. The polymerase chain reaction (PCR) method was used for amplification of the exon-1 region of the GDF9 gene with two pairs of primers. Analysis of genetic variation and single nucleotide polymorphisms (SNP) using MEGA software version X. Multiple alignment results showed that there are 2 nucleotides that have mutations, but not encode different amino acids. This results indicates that the mutation has association with litter size of two breeds. This finding shows that Lakor goats have polymorphism i.e SNP g.54C>T which is significantly associated with litter size. The analysis of GDF9 gene showed that all samples from the Two local Indonesian goat breeds (Kosta and Lakor) are  monomorphic. But, although both are monomorphic, geographical isolation still contributes to genetic drift within and between populations.
IDENTIFICATION OF GENETIC DIVERSITY CYTOCHROME OXIDASE SUBUNIT II (COII) MITOCHONDRIAL GENE AS GENETIC MARKER FOR ANISAKIS SPECIES IN Euthynnus affinis Prasetyarti Utami; Niken Satuti Nur Handayani; Rony Marsyal Kunda
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.2960

Abstract

ABSTRACT This study aimed to get specific genetic marker for Anisakis sp. identification on mackerel tuna using gene sequence cytochrome oxidase subunit II (COII) mitochondrial deoxyribonucleic acid (mtDNA) and to identify taxonomic affiliation between Anisakis sp. from Indonesia and others Anisakis sp. from GenBank database. This study started with sample collections at three fish auctions in Cilacap (Central Java), morphology classification, DNA isolation, and molecular based identification using polymerase chain reaction (PCR) and sequencing methods. Molecular based identification of Anisakis used gene amplification COII mtDNA as a cell target prior to sequence. Morphology characteristic results showed that Anisakis nematodes which infected mackerel tuna classified as type II L3 larvae. Molecular based identification showed significant result, which found 530 bp COII DNA gene fragment similar to target cell. Gene sequencing alignment results of COII Anisakis gene compared with GenBank showed 11 different nucleotide sites that can be used as genetic barcode for Indonesian Anisakis sp. This study showed that Anisakis sp. infected mackerel tuna in Java Sea is Anisakis physeteris and considered as zoonosis.
Co-Authors Frenly Marvi Selano Hery Wijayanto Ivan Mambaul Munir Jamhari Jamhari Maman Rumanta Marleny Leasa Niken Satuti Nur Handayani Pieter Kakisina Prasetyarti Utami Rini Widayanti Sandra Sukmaning Aji Slamet Diah Volkandari Slamet Diah Volkandari Sri Kurniati Handayani Ucu Rahayu Woro Danur Wendo