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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Medical Journal of Indonesia The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy
Agnes Kurniawan
Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta.
Agriculture, Biological Sciences & Forestry Environmental Science Health Professions Medicine & Pharmacology

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Detection of Cryptosporidium sp infection by PCR and modified acid fast staining from potassium dichromate preserved stool Kurniawan, Agnes; Dwintasari, Sri W.; Soetomenggolo, Herbowo A.; Wanandi, Septelia I.
Medical Journal of Indonesia Vol 18, No 3 (2009): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (421.766 KB) | DOI: 10.13181/mji.v18i3.354

Abstract

Aim To identify the frequency of Cryptosporidium infection in children below 3 years old by examining concentrated long term preserved stool using PCR detection of 18S rRNA gene and compared with modified acid fast staining technique.Methods Hundred eighty eight stools from children ≤ 3 years old were stored for 13 months in 2.5% K2Cr2O7 solution at 40C. Cryptosporidium oocysts were isolated by water-ether concentration technique. The concentrates were smeared onto object glass and stained with modified acid fast staining, and the rest of the concentrates were DNA extracted by freezing and thawing cycles and proteinase K digestion, then direct PCR was done to detect 18S rRNA gene.Result The proportion of positive stools for Cryptosporidium sp by acid fast staining from concentrated stools and 18S rRNA PCR were 4.8% and 34.6% respectively, which showed statistically significant difference.Conclusion The frequency of Cryptosporidium infection among children ≤ 3 years old was very high and stool storage in K2Cr2O7 for 13 months did not affect the PCR result. High prevalence of Cryptosporidium infection indicated high transmission in that area and the potential to be transmitted to other individuals such as the immunocompromised. (Med J Indones 2009;18:147-52)Key words: 18S rRNA, cryptosporidiosis
Treatment of Wuchereria kalimantani infection in Presbytis cristata with a single dose of CGI 18041 Supali, Taniawati; Rukmono, Bintari; Wibowo, Heri; Ekarina, Rita; Kurniawan, Agnes; Wardhani, Endang S.; Purnomo, Purnomo; Haerani, Tjahaya; Partono, Felix
Medical Journal of Indonesia Vol 6, No 1 (1997): January-March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.467 KB) | DOI: 10.13181/mji.v6i1.800

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[no abstract available]
Diagnosis and Management of Blastocystis Hominis Infection in Patient with HIV-AIDS Ginanjar, Eka; Kurniawan, Agnes; Hardjono, Teguh; Syam, Ari Fahrial; Pitoyo, Ceva Wicaksono
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 8 ISSUE 2 August 2007
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.036 KB) | DOI: 10.24871/82200757-62

Abstract

The incidence of AIDS/HIV infection has been increasing worldwide. Patients with AIDS/HIV infection is at high risk to get opportunistic infection which is often become life-threatening. Common infections associated with AIDS/HIV are tuberculosis (TB) infection and viral hepatitis. Commensal organism found in human body is actually not pathogenic. Blastocystis hominis is generally considered as commensal organism of intestinal tract and might cause opportunistic infection in patients with AIDS. We reported a case of young male patient with AIDS/HIV infection and evidence of opportunistic infection of Blastocystis hominis found in ascitic fluid along with concomitant lung TB and viral hepatitis. Patient was well-responded to treatment of B. Hominis. Keywords: AIDS/HIV infection, opportunistic infection, Blastocystis hominis
GENOTYPING OF TOXOPLASMA GONDII IN CEREBRAL AND OCULAR TOXOPLASMOSIS Mayashinta, Dearikha Karina; Halleyantoro, Ryan; Sari, Ika Puspa; Kurniawan, Agnes
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.022 KB) | DOI: 10.11594/jtls.08.03.02

Abstract

Toxoplasma gondii is an obligate intracellular protozoon, spread world-wide and capable of infecting birds and mammals. Genetic information on T. gondii type that causes human toxoplasmosis is limited. In this study, genetic analysis of SAG2 locus was used to determine the genotype of T. gondii from cases with cerebral and ocular toxoplasmosis in Indonesia. Genotype determination was done directly on the clinical samples. A number of 28 cerebrospinal fluid and 8 vitreous humor positively infected with T. gondii, underwent PCR-RFLP to classify each isolate into one of three genotypes of T. gondii. Type I was the most common found suggesting that cerebral and ocular toxoplasmosis in Indonesia is mostly caused by type I strain of T. gondii. 
Co-Authors Ari Fahrial Syam Bintari Rukmono Ceva Wicaksono Pitoyo Eka Ginanjar Endang S. Wardhani Felix Partono Halleyantoro, Ryan Herbowo A. Soetomenggolo I Ketut Suada Ika Puspa Sari Mayashinta, Dearikha Karina Purnomo Purnomo Rita Ekarina Septelia I. Wanandi Sri W. Dwintasari Taniawati Supali Teguh Hardjono Tjahaya Haerani