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All Journal JBIO: jurnal biosains (the journal of biosciences) Journal of Biology Education Jurnal Pendidikan Biologi Jurnal Pembelajaran dan Biologi Nukleus Jurnal Pendidikan Indonesia (Japendi)
Melva Silitonga
Universitas Negeri Medan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology

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BERAT BADAN DAN KADAR KOLESTEROL ORGAN-ORGAN DALAM AYAM BROILER YANG DIBERI PAKAN SUPLEMENTASI TEPUNG DAUN BANGUNBANGUN (Plectranthus amboinicus L. Spreng) Agatha Cecilia Sihite; Melva Silitonga
JBIO: jurnal biosains (the journal of biosciences) Vol 4, No 1 (2018): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v4i1.9789

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian pakan suplementasi tepung daun bangunbangun (TDB) (Plectranthus amboinicus L. Spreng) terhadap berat badan, berat organ dalam dan kadar kolesterol organ dalam ayam broiler. Jenis penelitian adalah eksperimental menggunakan Rancangan Acak Lengkap (RAL) Non Faktorial menggunakan 50 ekor ayam broiler CP 707. Pemberian TDB dilakukan setiap hari dengan mencampurkan TDB dengan pakan komersial dengan dosis 0%, 1%, 2%, 3% dan 4% selama 42 hari . Parameter yang diamati adalah berat badan, berat hati, berat usus halus, berat ampela, kadar kolesterol hati, kadar kolesterol usus halus dan kadar kolesterol ampela. Data yang diperoleh dianalisis menggunakan Anava satu jalur dengan menggunakan SPSS 21.0. Hasil penelitian menunjukkan bahwa suplementasi TDB 2% meningkatkan berat badan ayam secara signifikan, dan suplementasi TDB 1%, 3% dan 4% tidak berpengaruh . Suplementasi TDB 1% hingga 4% berpengaruh menurunkan berat hati secara signifikan. Suplementasi TDB 4% signifikan menurunkan kadar kolesterol hati, usus halus dan ampela.
KEMANJURAN IgY KUNING TELUR AYAM YANG TELAH MEMPEROLEH SUPLEMENTASI PIRIDOKSIN MENCEGAH KELAINAN OLEH Salmonella enteritidis Pasar Maulim Silitonga; Melva Silitonga; Meida Nugrahalia
JBIO: jurnal biosains (the journal of biosciences) Vol 5, No 3 (2019): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v5i3.14563

Abstract

Telah dilakukan penelitian untuk menguji kemanjuran IgY kuning telur ayam yang telah memperoleh suplementasi piridoksin dalam mencegah kelainan yang diakibatkan oleh infeksi bakteri S.Enteritidis. Telur dikoleksi dari ayam petelur yang telah diberi suplementasi piridoksin dosis 3 mg/kg dan diinjeksi dengan S. Enteritidis sebanyak 1,0 ml  (109sel/ml).  Pada uji kemanjuran IgY digunakan 10 ekor tikus putih dewasa yang dibagi menjadi 2 kelompok yang terdiri dari lima ekor setiap kelompok. Kelompok P diberi kuning telur selama 31 hari , kelompok K  (control)  tidak diberi kuning telur. Setelah perlakuan kuning telur selesai, semua tikus putih disuntik dengan S.Enteritidis 1,0 ml  (109sel/ml). Hasil penelitian menunjukkan bahwa IgY antidiare yang terkandung dalam kuning telur ayam yang telah memperoleh suplementasi piridoksin, cukup efektif mempertahankan kadar kalium dan albumin serum sebagaimana pada kondisi normal. 
EFEKTIFITAS BERBAGAI METODE SUPLEMENTASI PIRIDOKSIN MENGOPTIMALISASI PRODUKSI IMMUNOGLOBULIN Y (IgY) KUNING TELUR AYAM Pasar Maulim Silitonga; Melva Silitonga; Meida Nugrahalia
JBIO: jurnal biosains (the journal of biosciences) Vol 4, No 2 (2018): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v4i2.10417

Abstract

Telah dilakukan penelitian yang bertujuan untuk mengoptimalkan produksi immunoglobulin Y (IgY) kuning telur dengan metode suplementasi piridoksin.  Digunakan 12 ekor ayam betina dewasa (jenis Isa brown) siap bertelur, antigen yang digunakan toksoid tetanus.  Pemeliharaan dilakukan dalam kandang baterai selama 10 minggu. Selama percobaan, semua ayam diberi air minum secara ad libitum dan ransum komersil standar. Setelah 12 hari masa adaptasi, ayam diberi perlakuan suplementasi piridoksin dosis 3 mg/kg ransum dengan metode yang bervariasi yaitu via air minum (S1), mencampurkannya dalam ransum (S2) dan melalui suntikan intramuscular (S3). Semua ayam disuntik dengan antigen toksoid tetanus dosis 100 Lf yang diemulsikan dalam Freund’s adjuvant complete  yang diberikan secara intramuscular. Immunisasi ulang dilakukan dengan menggunakan freund’s adjuvant incomplete setelah dua, tiga dan empat minggu pemberian perlakuan suplementasi piridoksin.  Sampel telur diambil setelah 2 minggu injeksi antigen toksoid terakhir. Ekstraksi IgY kuning telur dilakukan dengan Metode PEG–Khloroform, Uji spesifitas IgY dengan uji AGP, Purifikasi IgY dengan FPLC, kadar IgY kuning telur ditentukan dengan metode Bradford. Hasil penelitian menunjukkan bahwa tidak ada perbedaan yang signifikan kadar IgY kuning telur ayam yang diberi suplementasi piridoksin via air minum, dicampur dalam ransum maupun via suntikan intravena. Suplementasi piridoksin pada ayam petelur memberikan rataan kadar antibodi / immunoglobulin yolk (IgY) kuning telur untuk ketiga jenis metode yang diaplikasikan sebesar 106,6 - 109,0 mg/ butir telur.
PENGARUH EKSTRAK ETANOL Plectranthus amboinicus Lour Spreng TERHADAP BERAT BADAN DAN BERAT RELATIF ORGAN TIKUS YANG DINDUKSI KANKER KULIT DENGAN DMBA Melva Silitonga; Erlintan Sinaga; Pasar Maulim Silitonga
JBIO: jurnal biosains (the journal of biosciences) Vol 7, No 2 (2021): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v7i3.23288

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol daun Plectranthus amboinicus terhadap  berat dan berat relatif organ dan berat badan tikus yang diinduksi kanker kulit dengan DMBA. Digunakan 24 ekor tikus dan dibagi menjadi empat kelompok yaitu K-, K+, T1 dan T2. K- yaitu kelompok kontrol diberi CMC 0.5%. K+  diberi DMBA 175 µg, T1 dan T2 diberi DMBA 175 µg dan EEP sebanyak 250 dan 500 mg/kg berat badan. Induksi DMBA diterapkan secara topical dibagian sisi posterior punggung dengan luas 3×3cm. DMBA dilarutkan dalam 0.1 ml aceton dioleskan. seminggu dua kali selama delapan minggu. Pada minggu ke sembilan, diberikan EEP setiap hari selama empat minggu pada kelompok T1 dan T2. Selama penelitian semua tikus diberi pakan dan minum secara ad libitum. Pada minggu ke 13 tikus dibunuh lalu dibedah untuk memperoleh organ ginjal, paru, hati dan limpa. Data yang diperoleh dianalisis dengan Anova. Hasil penelitian menunjukkan induksi kanker kulit dengan DMBA meningkatkan berat jantung, paru dan ginjal. Pemberian EEP menurunkan berat organ tersebut  menjadi sama dengan kontrol. DMBA meningkatkan berat relatif organ jantung, ginjal, paru-paru dan hati. EEP menurunkan berat relatif organ.  Pemberian DMBA menurunkan berat  badan mingguan pada tikus perlakuak K+, T1 dan T2. Pemberian EEP meningkatkan berat badan tikus yang diinduksi kanker kulit dengan DMBA
The Development of Animal Physiology Textbook Based on Higher Order Thinking Skills for Biology Department Students Indra Jaya Purba; Melva Silitonga; Idramsa Idramsa
Jurnal Pendidikan Biologi Vol 11, No 2 (2022): Jurnal Pendidikan Biologi
Publisher : Magister Pendidikan Biologi, Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpb.v11i2.33654

Abstract

The students’ higher order thinking skills were still low due to the learning system, such as the use of textbooks that have not led to an increase in higher order thinking skills. The availability of updated textbooks based on higher order thinking skills was needed in the university. The aim of this study was to develop a textbook based on higher order thinking skills on animal physiology course. The development model used in this study was the Thiagarajan (4D) development model which consisted of define, design, development and dissemination. However, the research stage carried out was only up to development. The developed textbook was validated based on three criterias, namely material, language and design. The developed textbook was also tested to students who have taken animal physiology course and obtained the students’ response. The result of validation by material expert was 86 in the category of “Very Good”, language expert was 88 in the category of “Very Good”, design expert was 87 in the category of “Very Good” and students’ response according to individually, small group and limited group were 86, 89 and 89 respectively. The whole students’ responses were categorized as “Very Good”. It can be concluded that the product can be used as a textbook in animal physiology course. This study is useful as a learning source for students and an alternative teaching material for lecturers, as well as improving the quality of animal physiology learning.
The Influence of Learning Style on Science Process Skills and Student Learning Outcome of Digestive System Material Githa Indriana; Melva Silitonga; Fauziyah Harahap
Jurnal Pendidikan Indonesia Vol. 2 No. 06 (2021): Jurnal Pendidikan Indonesia (Japendi)
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (276.476 KB) | DOI: 10.36418/japendi.v2i6.194

Abstract

This study aimed to analyze the learning style on science process skill and learning outcome class XI digestive system in State Senior High School of Kisaran, Asahan. This method applied a descriptive study with a quantitative approach. Samples were taken from four schools, each one class totaling 144 students by using purposive sampling. To obtain the data, the description test was used as a questionnaire to determine student learning styles. The high results are visual with a percentage of 38.19. Then the student science process skills instrument with indicators such as observing, classifying, interpreting, predicting, asking questions, hypothesizing, planning experiments, applying concepts, and communicating in biology learning. The results on science process skills of the digestive system material were categorized as good with a score of 82.06 and the highest indicator for observing obtained a mean score of 86.63.with a very good category and the lowest indicator for applying the concept obtained a score of 79.16 a good category. The learning result test obtained a score of 89,04with a very good category. The high score of C6 was obtained at 91.43. and lowest C5 obtained 72.22.
In vitro Growth of Cattleya sp Orchid from Leaf Explants with Growth Regulators Fauziyah Harahap; Itra Hariyadi; Melva Silitonga; Cicik Suryani; Syahmi Edi; Ayu Putri Ningsih
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 1 (2023): Jurnal Pembelajaran Dan Biologi Nukleus March 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i1.3945

Abstract

Using leaf explants, this research aimed at identifying the type of growth response that can be created and the optimal mix of media for the growth of Cattleya sp orchid. This was an experimental investigation employing the RAL method (completely randomized design) with a combination of 2.4-D (0;1; 2; 3 ppm) and Kinetin  (0;0.3;0.6 ppm) repeated three times. According to the results of this study, the growth regulators 2.4-D and kinetin  were unable to promote the development of all explants. Explants transferred to media containing polyvinylpyrrolidone (PVP) to prevent browning after 12 weeks of observation. Then the results of the study also found the emergence of shoots in the treatment P0 (control), P2 (2,4-D 0 + Kinetin  0.6 ppm), P8 (2,4-D 2 + Kinetin  0.6) and P11 (2.4- D 3 + K 0.6 ppm) in the 3rd week of observation, the appearance of callus at P5 (2.4-D 1 + Kinetin  0.6 ppm) in the 4th week of observation
Problem-Solving Ability with the Implementation Guided Discovery Model on Excretory System Material in Class XI of SMA Negeri 18 Medan Morina Wati; Melva Silitonga
Journal of Biology Education Vol 12 No 2 (2023): Agustus 2023
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v12i2.71589

Abstract

One of the demands of 21st-century learning is optimizing students' problem-solving abilities. The problem-solving ability of students at SMA Negeri 18 Medan is still low. One of the contributing factors to low problem-solving ability is the lack of application of learning devices and models that can activate students and are still teacher-centered. One learning model that can improve problem-solving skills is the guided discovery model. This study aims to analyze students' problem-solving abilities with the implementation of the guided discovery model, the implementation of guided discovery syntax in learning, and student responses to the guided discovery model on excretory system material. This type of research is quasi-experimental research with a pretest-posttest control group design. Sampling using random sampling technique, XI IPA 1 as guided discovery class and XI IPA 2 as direct instruction class. The instruments used consist of essay questions on problem-solving skills, syntax implementation observation sheets, and student response questionnaires. The data obtained are first tabulated, searched for averages, and then analyzed by normality tests and independent sample t-tests. From the results of the independent sample t-test, a significance value of 0.000<0.05 was obtained, indicating that students' problem-solving abilities with guided discovery models and direct instruction models differed significantly. The results showed that the problem-solving ability of grade XI students of SMA Negeri 18 Medan who were taught using the guided discovery model was very high (82.69), the implementation of the guided discovery model syntax with a percentage of 99%, and student responses to learning showed a positive response. Based on the results of the study, the guided discovery model is very appropriate to be used in training the problem-solving ability of grade XI students at SMA Negeri 18 Medan on excretory system material.
Co-Authors Agatha Cecilia Sihite Ayu Putri Ningsih Cicik Suryani Erlintan Sinaga Fauziyah Harahap Githa Indriana Idramsa Idramsa Indra Jaya Purba Itra Hariyadi Meida Nugrahalia Morina Wati Pasar Maulim Silitonga Syahmi Edi