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PENDEKATAN FENETIK TAKSONOMI DALAM IDENTIFIKASI KEKERABATAN DAN PENGELOMPOKKAN IKAN GENUS TOR DI INDONESIA Rahayu, Dwi Anggorowati; Nugroho, Endik Deni; Haryono, Haryono; Kurniawan, Nia; Azrianingzih, Rodiyati
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 11, No 1 (2014): Prosiding Seminar Nasional XI Biologi
Publisher : Universitas Sebelas Maret

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Abstract

Tor genus is a local Indonesian freshwater fish, and endangered species. The urgent conservation efforts should be made to determination of relationship based on phenetic approach. This is because the taxonomical position and classification of this fish were unclear. The data used in this study were meristic, general of morphology, and special character to determinate up to the species level. The special characters of this fish were the existence and size of the median lobe. Samples were taken from Banyu Biru lake (Lokal name were Sengkaring and Tambra), as well as the related species, They were consists of Tor duoronensis from Padang, Tor Tambraides from West Borneo, and Tor soro were obtained from North Sumatra. Fenogram formed based on UPGMA method. According to the phenogram, it was shown that 3 related groups of Tor Genus laid from near to far distance based on their relationship, 2 apomorphy and 4 automorphy group. The first consists of two subklad namely Tor duoronensis and Sengkaring with their similarity index 100%, and Tambra was sister clad of Tor duoronensis supported by the similarity index of 92.9 %. The second Subklad consists of Tor tambra and Tor soro (Apomorfi B) with a similarity index 59%, while the furthest distance Tor tambraides with similarity index of 45, 625%. Tambra and Sengkaring showed a grouping pattern that closely related to Tor duoronensis based on seven main distinguishing morphometric features. Seven main distinguishing features were consist of: size of median lobe, SL (standard length), MXBL (maxillary barbels lenght), CPL (caudal peduncle lenght), PDL (pre dorsal lenght), IW (interorbital width) and SNL (snout lenght). Furthermore, the results of morphometric analysis showed that Sengkaring and Tambra was indeed not a member of Tor Tambraides and Tor soro species due to significant difference of body size. Keywords: Tor, Relationship, Phenetic, Morphology, Special Character, Meristric, Conservation
Virtual Screening of Mimosa pudica Secondary Metabolites as Hyaluronidase B Potential Inhibitor to Prevent Vespa velutina Venom Spreading Rijalullah, Muhammad Asyraf; Pramudya, Muhammad Alif Imam; Maisuroh, Dalilatul; Zain, Dhiyaa Syahlaa Bianca Febrinnisa; Kurniawan, Nia; Fatchiyah, Fatchiyah
JSMARTech: Journal of Smart Bioprospecting and Technology Vol 2, No 3 (2021)
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2021.002.03.96

Abstract

Vespa velutina, also known as the Yellow-legged hornet, is a wasp species native to Asia with a large distribution area in Indonesia. Hyaluronidase B in a wasp venom acts as a "spreading factor", which is the key at the beginning of envenomation. Shameplant (Mimosa pudica), a common plant in Indonesia, has shown the potential to be a hyaluronidase B inhibitor. This study aimed to analyze the potential of secondary metabolites in Shameplant as an inhibitor of V. velutina Hyaluronidase B base on their molecular interactions and as a topical drug base on physicochemical characteristics. In silico computational studies is performed to predict the binding modes of M. pudica compounds and hyaluronidase B enzyme. The secondary metabolites were retrieved from the PubChem database and screened using SwissADME. The seven metabolite compounds were docked with Hyaluronidase B and hyaluronan by HEX Cuda 8.0.0 program. Hyaluronidase B was also docked with its native ligand (hyaluronan) to validate the docking study. Three dimensional and 2D views were then evaluated using Discovery Studio 2016. Results of this study are all compounds do not have the same molecular interaction with the control. It defines no inhibition of the interaction on the active side. Mimopudine is the most potent inhibitor of hyaluronidase B based on its binding energy. While, jasmonic acid is the only compound that meets the physicochemical parameter of the topical drug.
Pengaruh Hormon Hipofisa dan Ovaprim Terhadap Ovulasi Serta Pengaruh Pakan Terhadap Pertumbuhan Berudu Katak Fejervarya cancrivora Ardyah Ramadhina Irsanti Putri; Nia Kurniawan; Agung Pramana W.M
Biotropika: Journal of Tropical Biology Vol 1, No 5 (2013)
Publisher : University of Brawijaya

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Katak Fejervarya cancrivora merupakan kelompok dari kelas amfibi yang habitatnya sangat dipengaruhi oleh perubahan kondisi habitat dan aktivitas manusia. Salah satu upaya untuk mempertahankan keanekaragaman hayati yaitu memanfaatkan teknologi reproduksi buatan dengan melakukan induksi pematangan gonad menggunakan hormon hipofisa dan ovaprim. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian hormon hipofisa dan ovaprim terhadap pematangan gonad dan keberhasilan fertilisasi pada katak Fejervarya cancrivora, serta mengetahui pengaruh perbedaan pemberian pakan pada pertumbuhan berudu katak Fejervarya cancrivora. Injeksi dilakukan pada katak betina yang  hampir matang gonad dengan total dosis injeksi 250 µl secara intraperitonial. Setelah dipijahkan dan difertilisasi, telur dipelihara sampai menetas dan diberikan dua perlakuan pakan, yaitu pakan bayam  rebus (P1) serta  pakan bayam rebus dan kuning telur rebus (P2). Hasil penelitian menunjukkan pada perlakuan injeksi hipofisa dihasilkan telur 632 butir sel telur dan berhasil menetas 65,67%  dan perlakuan injeksi ovaprim sebanyak 108 butir sel telur dan dapat menetas 75,92%. Hasil  perlakuan P1 dan P2 pada kedua jenis injeksi tidak terdapat perbedaan panjang (p>0,05) namun terdapat perbedaan berat (p<0,05) pada perlakuan selama masa metamorfosis. Persentase jumlah berudu yang berhasil bermetamorfosis sempurna pada perlakuan injeksi hipofisa sebanyak 41,12%, sedangkan pada perlakuan injeksi ovaprim sebanyak 31,67%.
GENETIC ANALYSIS AND MOLECULAR INTERACTION OF UGPase IN SOME TUBER PLANTS RELATED TO GLUCOMANNAN PRODUCTION Badiul Ulum; Estri Laras Arumingtyas; Nia Kurniawan
Biotropika: Journal of Tropical Biology Vol 1, No 3 (2013)
Publisher : University of Brawijaya

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UGPase is an enzyme that affect the conformation of polysaccharide with β 1-4 bond. There are some differences interaction of UGPase enzyme with UDP-Glucose substrate in some tuber plants. So, the aim of this research are to observe the interaction of UGPase and UDP-G in some tuber plants and to observe the relationship among tuber plant based on UGPase sequence. Result of molecular docking shows the catalytic domain interaction of UGPase and UDP-G in substrate binding loop, lower energy free obtain from A. konjac is -5.06 kcal/mol, following by I. batatas and S. tuberosum is -4,60 dan -3,73 kcal/mol respectively. Interaction was stabilize by some interaction type, there are electrostatic and hydrogen in A. konjac and I. batatas, and hydrophobic in S. tuberosum. Sequence alignment of UGPase shows I. batatas and S. tuberosum relatively close than to A. konjac.   Key words: interaction, UDP-Glucose, UGPase
Understanding Snake Bite Cases Pattern Related to Volcano-Seismic Activity: An Evidence in Bondowoso, Indonesia Nia Kurniawan; Ahmad Muammar Kadafi; Agung Sih Kurnianto; Franky Ardiansyah; Tri Maharani
Biotropika: Journal of Tropical Biology Vol 5, No 3 (2017)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2017.005.03.8

Abstract

Bondowoso located in a valley of Raung mountain roots in East Java, Indonesia. The eruption of Mt. Raung in 2015 not only cause loss of life, but also underlining the fact of unusual Snake Bite Cases (SBC), as recorded in the dr. Koesnadi Hospital. We conducted a thorough analysis of the SBC in 2015 along with the survey on all the incident location in January to March 2016. This study represented an improvement on the population of venomous snake in the agriculture area, and reached the highest peak before the eruption. The presence of continuous vegetation at the edge of watersheds become green corridor that functioned as the movement track for snakes in order to avoid the volcano effect.
Eksplorasi Jenis-Jenis Amfibi di Kawasan OWA Cangar dan Air Terjun Watu Ondo, Gunung Welirang, TAHURA R.Soerjo Qothrun Izza; Nia Kurniawan
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
Publisher : University of Brawijaya

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Penelitian ini bertujuan untuk mengetahui jenis amfibi yang dapat ditemukan di kawasan OWA Cangar dan Air Terjun Watu Ondo, TAHURA R.Soerjo, mengetahui karakter habitatnya, serta mengetahui jenis yang berpotensi sebagai bioindikator. Pengambilan data mulai November 2013 hingga Maret 2014 menggunakan metode  Visual Encounter Survey (VES), total usaha 13 jam, 3 pengamat. Lokasi pengambilan data pada ketinggian 1400-1700 mdpl. Faktor abiotik yang diukur meliputi  pH air, suhu (udara & air), dan kelembapan udara. Ditemukan lima jenis amfibi yang seluruhnya tergabung dalam ordo Anura dari lima famili berbeda, yakni katak pohon emas (Philautus aurifasciatus Schlegel, 1837), katak serasah (Leptobrachium hasseltii Tschudi, 1838), bangkong kerdil (Limnonectes microdiscus Boettger, 1892), kongkang jeram (Huia masonii Boulenger, 1884), dan kodok buduk (Duttaphrynus melanostictus Schneider, 1799). Tidak ditemukan satupun spesies dari ordo Caudata maupun Gymnophiona. P. aurifasciatus kerapkali ditemukan pada tumbuhan Psychotria sp., Dypsis lutescens, dan Ficus sp.. L. microdiscus mempunyai habitat daerah hutan dari dataran rendah hingga tinggi. Habitat H. masonii selalu terkait dengan sungai berbatu yang berarus deras dan air jernih. L. hasseltii dapat ditemukan di daerah tinggi di antara serasah hutan. D. melanostictus mempunyai habitat di dekat hunian manusia atau wilayah terganggu. Spesies yang berpotensi besar sebagai bioindikator adalah P. aurifasciatus, H. masonii sebagai indikator perairan jernih, dan D. melanostictus sebagai indikator wilayah terganggu. Kata kunci : Amfibi, Cangar, ketinggian, VES 
The Predicted Distribution of Javan Munia (Lonchura leucogastroides) in Indonesia based of Behavior Analysis in Kalibaru, Banyuwangi, East Java Agung Sih Kurnianto; Nia Kurniawan
Biotropika: Journal of Tropical Biology Vol 1, No 1 (2013)
Publisher : University of Brawijaya

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Javan Munia is one of the common species of birds that found in west area of Indonesia (Sunda land). This species is endemic to Indonesia (Lombok, Bali, Java, and South Sumatra). The large population, especially during the harvest season makes this bird became a major pest for agriculture. This research aims to determine the adaptive behaviors of Javan Munia that become major reasons of widely spreads and the influence of these factors to the distribution. Observations made in Kalibaru, Banyuwangi, East Java, which is a mostly agricultural area. Any form of behavior in the territorial area (nesting and courtship areas) and range area (where colonize and feed) were observed periodically in the past 12 months. Sunbathing is known as a activity that undertaken by many species of birds, including the Javan Munia. This munia has specific mating and nest construction behaviour. The development of Javan Munia is very quickly, in 40 days, child will become a new adult. Javan Munia is very easy to adapt to the surrounding environment, including the used of oil palm (Elais guineensis) fiber and paper receipts as nest material. There are a possibility of invasive occurrence to the entire island of Sumatra by this species. It is quite possible because of the opening of oil palm plantations and settlements continue, until Aceh. The spread possibilty to east end of distribution (Lombok) can occur up to Nusa Tenggara Island, because of short distances between the islands and similiar vegetation circumstances.
Struktur Komunitas Vertebrata dan Invertebrata Air pada Petak Sawah Organik di Kecamatan Lawang, Kabupaten Malang Herlin Aprilia Kartini; Nia Kurniawan
Biotropika: Journal of Tropical Biology Vol 1, No 4 (2013)
Publisher : University of Brawijaya

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Abstract

Sistem pertanian organik diharapkan tidak hanya menghasilkan produk pertanian yang baik namun juga tidak menimbulkan dampak buruk bagi lingkungan dan organisme yang hidup disekitar. Tujuan penelitian ini adalah untuk mengetahui diversitas dan struktur komunitas vertebrata dan invertebrata air pada petak sawah organik di Kecamatan Lawang, Kabupaten Malang. Penelitian ini bersifat deskriptif eksploratif dengan pengambilan data yang dilakukan sejumlah 5 kali. Metode pengambilan sampel vertebrata yang digunakan adalah ‘visual encounters surveys’, sedangkan hewan invertebrata diamati dengan membuat plot ukuran 1m2 pada petak sawah. Petak sawah yang diamati adalah sejumlah empat titik, dengan waktu pengamatan pada pagi hari sekitar pukul 06.00-11.00 WIB. Hasil pengamatan vertebrata air diperoleh 4 spesies meliputi 3 jenis ikan dan 1 jenis amfibi. Vertebrata perairan yang memiliki Indeks Nilai Penting (INP) tertinggi adalah katak sawah Fejervarya spp.. yaitu sebesar 109,9. Sedangkan invertebrata yang ditemukan antara lain berbagai jenis siput, arthropoda dan kerang. Invertebrata yang memiliki INP tertinggi adalah  siput Lymnaea truncatula dengan INP sebesar 59,98. Nilai indeks diversitas Shannon-Wienner (Hi) hewan air pada lokasi penelitian memiliki nilai yang sedang ( 1 - 3) yaitu vertebrata air sebesar 1,37 dan invertebrata air sebesar 2,38.   Kata Kunci: invertebrata sawah organik, struktur komunitas, vertebrata
Effect Variations of Fish Food on Growth in Catfish. (Clarias Garipinus) Lingga Nugraha; Nia Kurniawan
Biotropika: Journal of Tropical Biology Vol 1, No 3 (2013)
Publisher : University of Brawijaya

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ABSTRAK This study was carried out faculty Mathematics UB Malang 1 June 2012. This study aims to determine the effect of variations of food to African catfish (Clarias Gariepinus) using pests (Gold snails) and poultry waste (feces gemak) on the growth of channel catfish as well as to reduce the cost of purchasing an expensive artificial feed, so need to note the retention and protein efficiency ratio of fish needs every day. The variables were observed in this study was the weight, length, body color. The study was conducted using completely randomized design (CRD) with three treatment that feeding by comparing the artificial fish feed plant, fish feed made ​​with gold snails and artificial feed with poultry manure with each ratio 100:0, 70:30, 70:30 @ weight of each fish and each made ​​repeated 4 times, with other factors such as pH equated, light intensity, water temperature, water quality, oxygen levels. The results showed that there was no significant difference between the treatment given to the growth of the weight and length of the fish, but mixed with dung feeding quail in the ratio of 70: 30 more profitable because the costs were less. Feeding pellets mixed with the ratio of 70:30 keongmas also beneficial although slightly larger than quail dirt. Although the number of calories produced is less than the controls, but not significantly different, and the provision of a varied diet had no effect on the shape and color of the fish where the fish are shown color remains black and shiny, which means normal. So dirt and keongmas quail can be a safe alternative to feed and to reduce maintenance costs.   Keywords: Lele Dumbo, food, growth, color  
Eksplorasi Ektoparasit Pada Ikan Famili Cyprinidae Di Kolam Rumah Makan Wilayah Malang Raya Karina hanum wardany; Nia Kurniawan
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
Publisher : University of Brawijaya

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Ectoparasites were common found on fishes. Ectoparasites often infect parts of the fins, scales, operculum and gills in fish. This research aims to determine the types of ectoparasites on fish family Cyprinidae and the influence of environmental conditions on the number of ectoparasites. This research was conducted in three pools restaurant in Malang Raya region, then measured abiotic factors of pool water. Fish as the host of ectoparasites, taken using nets and anesthetized by using 3 phenol chloroform. Fish scales and fins were observed. Ectoparasites that attach to the parts taken and put in 70% alcohol. Ectoparasites observed and identified under a microscope. Measurement data of water quality and quantity ectoparasites were analyzed using SPSS 16 program. Argulus japonicus is a species of ectoparasite was founds in B Batu restaurant and I Dau restaurant. While ectoparasites found in G Malang restaurant was Lernaea cyprinacea. Argulus japonicus and Lernaea cyprinacea were exotic species from Japan. The entry of ectoparasites caused by introduction of Koi fish to Indonesia. The results showed that there were significant differences in the number of ectoparasites I Dau with B Batu and G Malang restaurants respectively. The correlation result between the number of ectoparasites and the physico-chemical factors were showed that the pool water DO levels were positively correlated to the number of ectoparasites.   Key word: Ectoparasites, fish family Cyprinidae, restaurant pond at Malang Area, SPSS 16
Co-Authors Achmad Dadang Burhanuddin Adityas Arifianto Agung Nugroho Agung Pramana W.M Agung Pramana W.M Agung Pramana Warih Marhendra Agung Sih Kurnianto Agung Sih Kurnianto Agung Sih Kurnianto Agus Nurrofik Agus Suryanto Aguung Sih Kurnianto Ahmad Muammar Kadafi Amin Setyo Leksono Amir Hamidy AMIR HAMIDY Andri Maulidi Andri Maulidi Anggun Sausan Firdaus Ardiansyah, Franky Ardiantoro, Ari Ardyah Ramadhina Irsanti Putri Ardyah Ramadhina Irsanti Putri Arief Sugiharto Asmaul Khusnah Asmaul Khusnah Azrianingzih, Rodiyati Azrianingzih, Rodliyati Badiul Ulum Badiul Ulum Bagus Priambodo Bayu Hendra Prakosa Catur Retnaningdyah Djong Hon Tjong Dwi Anggorowati Rahayu Elsa Mufti Endik Deni Nugroho Estri Laras Arumingtyas Faldy Alifianto Fatchiyah Fatchiyah Fauzi, Muhammad Alif Firdaus, Anggun Sausan Fitra Arya Dwi Nugraha Fitra Arya Dwi Nugraha Franky Ardiansyah Haryono Haryono Herlin Aprilia Kartini Herlin Aprilia Kartini Imti Yazil Wafa Karina hanum wardany Karina hanum wardany Karuniawan Puji Wicaksono Kholilah, Tsaniyah Nur Kurnianto, Aguung Sih Lingga Nugraha Luchman Hakim Lutfita Fitriana Lutviyana Rahman Lutviyana Rahman Maharani, Tri Mahmudul Hasan, Mahmudul Maisuroh, Dalilatul Masayuki Sumida, Masayuki Mohamad Nasirudin Mumpuni Mumpuni Nashi Widodo Nobukazu Nakagoshi Pramudya, Muhammad Alif Imam Qothrun Izza Qothrun Izza Rijalullah, Muhammad Asyraf Rudi Candra Aditama Rudi Candra Aditama Shima Ramadani Sugiharto, Arief Takeshi Igawa, Takeshi Tesri Maideliza Tri Maharani Vendy Adrinanda Vendy Adrinanda Zain, Dhiyaa Syahlaa Bianca Febrinnisa