Rochmah Kurnijasanti
Department Of Veterinary Basic Medicine, Faculty Of Veterinary Medicine, Universitas Airlangga

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ISOLATION OF Streptomyces sp. FROM LAPINDO MUD SOIL, SIDOARJO, EAST JAVA PROVINCE, INDONESIA AS A LARVICIDE CANDIDATE AGAINST Aedes aegypti Subagyo Yotopranoto; Rochmah Kurnijasanti; Etik Ainun Rohmah
Folia Medica Indonesiana Vol. 53 No. 2 (2017): JUNE 2017
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.549 KB) | DOI: 10.20473/fmi.v53i2.6355

Abstract

Streptomyces sp. is a bacterium that can live in many kind of habitations e.i. marine, sea sponge, coastal area, soil, desert soil, river bank etc. Many antibiotics and secondary metabolites are produced by this bacterium. Several secondary metabolites of the bacterium can be used as an insecticide against insects including mosquito larva.The purpose of this study was to search and isolate until species of Streptomyces sp. from Sidoarjo Lapindo mud soil in East Java province that can be used as a larvicide against Aedes aegypti larva, the dengue haemorrhagic fever vector.The method of study was to collect several mud soil samples from Sidoarjo Lapindo. The collected samples were cultured in ISP-4 media for producing mix-cultures. Then, to isolate the suspected colony of Streptomyces sp. by culturing again in several replications on ISP-4 media in petri-dish. The pure isolates were cultured in ISP-4 slant media. There were could be obtained eight pure isolates of Streptomyces sp.The characterization of 16S rRNA of Streptomyces sp. was done in order to determine the species. DNA isolation was done and followed by DNA sequencing, then compared to Gene Bank with BLAST program. The results showed that the sequence nucleotide bases of Streptomyces Sp-D6 had high similarity to Streptomyces sp. 171524, beside the sequence nucleotide bases of Streptomyces Sp-D7 and Sp-D9 had high similarity to Strepto-myces sp. ACT-01578 and ACT-175695. These three species can be used as larvicide candidate against Ae. aegypti.
Potensi Ekstrak Etanol Biji Cucurbita moschata terhadap Kadar Malondehaldehid Mencit Model Diabetes Suwanto, Suwanto; Gustomi, Mono Pratiko; Kurnijasanti, Rochmah
Indonesian Journal of Human Nutrition Vol 7, No 1 (2020)
Publisher : Jurusan Gizi, Fakultas Kedokteran, Universitas Brawijaya Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.ijhn.2020.007.01.3

Abstract

Abstrak Kebutuhan akan kesehatan diharapkan pada semua orang, namun kesehatan harus perlu dijaga agar tidak terpapar adanya radikal bebas yang berlebihan, karena akan memicu timbulnya penyakit diabetes mellitus disertai adanya peningkatan kadar malondehaldehid. Pencegahan radikal bebas yang berlebihan dapat memanfaatkan biji Cucurbita moschata. Antioksidan pada biji Cucurbita moschata berperan dalam mengatasi dan mencegah adanya stres oksidatif pada penderita diabetes mellitus. Penelitian bertujuan untuk mengetahui potensi ekstrak etanol biji Cucurbita moschata terhadap kadar malondehaldehid mencit model diabetes. Metode penelitian eksperimental dengan desain post test control group design, mencit jantan sebanyak 24 ekor umur 1 bulan dengan berat badan 20-30 gram, dibagi menjadi 6 kelompok: kontrol negatif, kontrol positif, kontrol metformin, dan  kelompok perlakuan ekstrak etanol biji Cucurbita moschata dosis 180, 360 dan 720 mg/kg BB diberikan secara sonde oral selama 14 hari. Pengamatan terhadap kadar malondehaldehid. Hasil penelitian pemberian ekstrak etanol biji Cucurbita moschata pada semua dosis kelompok perlakuan dapat menurunkan kadar malondehaldehid (Kruskal Wallis p 0,04 < 0,05), sedangkan dosis yang lebih efektif sebanyak 360 mg/kgBB. Kesimpulan semua dosis kelompok perlakuan dapat menurunkan kadar malondehaldehid, sedangkan dosis yang lebih efektif sebanyak 360 mg/kgBB. Kata kunci: Cucurbita moschata, malondehaldehid, diabetesAbstractEverybody expects of being healthy, but health must be maintained to avoid excessive free radical exposure since it will trigger the onset of diabetes mellitus that is accompanied by an increase in the malondialdehyde level. Prevention of excessive free radicals can be done by utilizing Cucurbita moschata seeds. Antioxidants in Cucurbita moschata seeds play a role in overcoming and preventing oxidative stress in people with diabetes mellitus. The study aimed to determine the potential of ethanol extract of Cucurbita moschata seeds on the malondialdehyde level in diabetic mice. This study applied an experimental research method with a posttest control group design. The subjects were 24 male mice aged one month with a bodyweight of 20-30 grams and were divided into six groups: negative control, positive control, metformin control, and treatment groups of ethanol extract Cucurbita moschata seed doses of 180, 360, and 720 mg/kgBW given orally for 14 days. The results of the study showed that ethanol extract of Cucurbita moschata seeds on all treatment doses could reduce the malondialdehyde level (Kruskal Wallis p 0.04 <0.05), but the most effective dose was 360 mg/kgBW. In short, all treatment doses can reduce the malondialdehyde level, but the most effective dose is 360 mg/kgBW. Keywords: Cucurbita moschata, malondialdehyde, diabetes
PENGARUH PEMBERIAN EKSTRAK KULIT SEMANGKA (Citrullus lanatus) TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA TIKUS (Rattus norvegicus) DENGAN PAPARAN SUHU PANAS Lailatul Rohmah; Indah Norma Triana; Agus Sunarso; Suherni Susilowati; Nove Hidajati; Rochmah Kurnijasanti
Ovozoa : Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.302 KB) | DOI: 10.20473/ovz.v7i2.2018.131-136

Abstract

High temperatures lead to oxidative stress which can decrease the quality of spermatozoa. Watermelon’s rind have an ability for antioxidants effect that can reduce sperm’s damages that results by role explanation of free radicals. This study aimed to determine the effect of extract of watermelon’s rind on the motility and viability of sperm on male rat exposed to heat. This experiments use rat as animals experiment with five treatments and four repetitions. Five treatments are: P0: without exposured to 40˚C of temperature and without treatment by extract watermelon’s rind, P1: was exposured to 40˚C of temperature and without treatment by extract watermelon’s rind, P2: was exposured to 40˚C of temperature and treatments by extract watermelon’s rind dosage 20 mg/rat/day, P3: was exposured to 40˚C of temperature and treatments by extract watermelon’s rind dosage 40 mg/rat/day, and P4: was exposured to 40˚C of temperature and treatments by extract watermelon’s rind dosage 80 mg/rat/day. The treatment was done for 52 days. The results, P4 have the best effect that can be increase the motility and viability of rat’s sperm (Rattus norvegicus) exposed to heat.
THE EFFECTS OF BANANA STEM (Musa paradisiaca var. sapientum) EXTRACT ON HISTOPATHOLOGIC GASTRIC OF RATS INDUCED BY INDOMETACHIN Rochmah Kurnijasanti; Arninda Alfiani Putri
Folia Medica Indonesiana Vol. 52 No. 4 (2016): OCTOBER - DECEMBER 2016
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.916 KB) | DOI: 10.20473/fmi.v52i4.5470

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This research aims to find out the administration effect of Ambon banana stem extract (Musa paradisiaca var. sapientum) to prevent gastric damage and observe histopathology of rat’s gastric induced by indomethacin. This research used 30 male rats age 8-12 week having 150 gram weight. They were selected randomly and divided into five groups. Negative control (K-) was given 0.5 ml CMC Na 0.5 % for 9 days and 0.5 ml corn oil was given on 10th. Positive control (K+) was given 0.5 ml CMC Na 0.5% for 9 days and then given Indomethacin emultion 5 mg/0.5 ml/150 g BW once on 10th day, and the other groups were given Ambon banana stem extract for (P1) 20 mg/150 g BW, (P2) 40 mg/150 g BW and (P3) 80 mg/150 g BW for 9 days. Afterwards, they were given Indometachin emultion 5 mg/150 g BW once on 10th day. Each gastric specimen was processed and histopathological changes were observed. Scoring of mucosa epithelium erosion and hemorrhagic, as qualitative data, was analyzed by Kruskall-Wallis test and continued by using Z test. The result shows that Pisang Ambon’s stem extract reduce significantly in gastric mucosa epithelium erosion and hemorrhagic induced by Indomethacin (p<0.05).
The Anthelmintic Activity Of Etanol Extract of Porang Tubers Against Fasciola gigantica In Vitro Firanda Agustina; Rochmah Kurnijasanti; Lucia Tri Suwanti; Nunuk Dyah Retno Lastuti; Kadek Rachmawati; Lilik Maslachah
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31235

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This study aims to determine the anthelmintic activity ethanol extract of porang tubers (A. oncophillus) against F. gigantica worms in vitro. There were five treatments and each treatment was done in five replications and used 10 F. gigantica. The treatments of this research were K- with CMC Na 1%, K+ with Albendazole 2.4 mg/ml, P1 with extract concentration 5%, P2 with extract concentration 10%, P3 with extract concentration 20%. The results showed that the extract of Porang tuber (A. oncophillus) had an anthelmintic effect against F. gigantica worms in vitro. In the extract with a concentration of 20%, there were anthelmintic properties that almost the same as Albendazole. The higher the extract concentration, the higher the anthelmintic properties. The longer the immersion time, the higher the number of dead worms. The morphological changes was evaluated by light microscopic examination and the results showed many histopathological changes on the morphology of F. gigantica. The results indicate that the possible use of the tubers as a potential anthelmintic against F. gigantica.
Pengaruh Persentase Pertukaran Air pada Pertumbuhan Gracilaria verrucosa dalam Budidaya Bak Terkontrol [The Effect Of Percentage Water Replacement On Growth Of Gracilaria verrucosa In Controllable Tanks] Rr. Juni Triastuti; Raindra Daksina; Rochmah kurnijasanti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 1 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.229 KB) | DOI: 10.20473/jipk.v2i1.11675

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AbstractGracilaria is a species of red algae which has potentially to culture. Generally, the culture is done in ponds, but today some ponds are use for housing and the pond number are decreasing, and the other hand the water quality of ponds is contaminated by industrial wasted. To overcome these problems, the solution is doing culture in a controllable tanks, no large space is needed, it can do in house, no seasonal influences and the supporting factors like o onutrient, pH, light intensity, temperature (C), salinity (/ ) and water circulation are easily to control. The purpose of oo this research is to determine the effect of water replacement on growth and the total number of chlorophyl a on Gracilaria verrucosa. This research was held at June 19 th 2009 – July 22 th 2009 in Laboratorium Kering, Fakultas Perikanan dan Kelautan and as the comparison is Desa Pulukerto Kecamatan Kraton, Kabupaten Pasuruan, Jawa Timur. The growth measurement in Public Health Veteriner Departement Faculty of Medical Veterinary Airlangga University Surabaya. The chlorophyll a measurement was held in Patology Clinic Departement Faculty of Medical Veterinary Airlangga University Surabaya. This research use Fully Randomized Design method with 5 treatments : in treatment A there was no water replacement (0%), in treatment B the replacement was 25%, replacement in treatment C was 50%, treatment D was 75% and in treatment E, all the water taking out from the tank was 100% replaced by new water from the reservoir, replicated four times. The growth data (heavy and length), with Analysis of Variance (ANOVA) and continueted with Duncan Multiple Range Test.
The Effectiveness of Ethanol Extract of Red Betel Leaf (Piper crocatum) Againts Mortality of Boophilus microplus Larvae in Vitro Meta Aprilia; Poedji Hastutiek; Rochmah Kurnijasanti; Lucia Tri Suwanti; Mohammad Sukmanadi; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.694 KB) | DOI: 10.20473/jops.v3i1.16429

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The aims of this study is to know the effectiveness of ethanol extract of red betel leaf (Piper crocatum) against mortality of Boophilus microplus Larvae in Vitro, as well as knowing lethal concentration 50 (LC50) and lethal concentration 90 (LC90). Method that used in the research was completely randomized design. There were five treatments and each treatment was done in five replications. This research used ten Boophilus microplus larvae in each treatment for all replication. The observation and recording of mortality was done at 1, 2, 3, 4, and 5 hours. Boophilus microplus larvae were declared dead if there was no movement. The obtained data was analyzed using ANOVA factorial. The result of this research show that ethanol extract of red betel leaf (Piper crocatum) has effectiveness against mortality of Boophlius microplus larvae in Vitro. In the extract 10% concentration, there is effectiveness that almost the same as Noticks®. The higher concentration of the extract, the higher the effectiveness against mortality lavae.In probit analysis show that LC50 achieved by concentration 5,4% or 2.700mg/50ml and LC90 achieved by concentration 11,3% or 5.650mg/50ml.
PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER SUSU SKIM TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN Maulinda Agchirilia Vladika; Sunaryo Hadi Warsito; Suherni Susilowati; Tatik Hernawati; Rochmah Kurnijasanti; Tjuk Imam Restiadi
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.119 KB) | DOI: 10.20473/ovz.v8i2.2019.116-121

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The purpose of this study was to determine the effect and the best concentrasion of green tea extract in skim milk diluent for motility, viability, and intact plasma membrane Sapudi sheep spermatozoa that was stored 50C. The semen was divided into four groups: skim milk  diluent; 0,05 mg green tea extract in skim milk diluent; 0,10 mg green tea in skim milk diluent and 0,15 mg green tea extract in skim milk diluent. Spermatozoa quality was observed first day until fifth day after diluent. The data obtained was analyzed with the analysis of variance one way ANOVA. The result showed that the percentage of motility, viability and intact plasma membrane spermatozoa at the 5th day after diluent showed a significant differece (p<0,05) between 0,05 mg green tea extract in skim milk diluent; 0,10 mg green tea in skim milk diluent and 0,15 mg green tea extract in skim milk diluent. Motility at the 3th day after diluent respectively 46,67b ± 2,582; 43,33bc ± 4,028; 40,00ab ± 3,162; and 37,50a ± 2,739. Viability  at the 3th day after diluent respectively are 79,50b ± 1,643; 77,67b ± 3,327; 77,00ab ± 3,230; and 73,83a ± 3,061. Intact plasma membrane at the 3th day after diluent respectively are 50,17b ± 2,483; 47,00ab ± 3,899; 46,83ab ± 2,858; and 46,00a ± 3,286. The conclusion of this study was the green tea extract of 0,05 mg in milk skim diluent could increase the percentage of sperm quality until 5th day after diluent.
PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER SUSU SKIM KUNING TELUR TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN Wahyu Retno Swari; Emy Koestanti Sabdoningrum; Wurlina Wurlina; Suherni Susilowati; Rochmah Kurnijasanti; Erma Safitri
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.407 KB) | DOI: 10.20473/ovz.v8i2.2019.122-126

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The purpose of this study was to determinated the effect and the best concentration of greentea extract in skim milk and egg yolk diluent for quality sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups; skim milk and egg yolk diluent, 0,5% green tea extract in skim milk and egg yolk diluent, 0,1% green tea extract in skim milk and egg yolk diluent, and 0,15% green tea extract in skim milk and egg yolk diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after being diluted. The data obtained was analyzed with the Analysis of Variant (ANOVA), followed by multiple range test duncan. The results showed that the highest percentage of motility, viability and intact plasma membrane derived from green tea extract 0,15% on skim milk and egg yolk diluent  for 1 day storage is 86.66b ± 2.58, 92.00b ± 1.78, and 74.16b ± 3.25. The lowest percentage of motility, viability and intact plasma membranes was obtained from the skim milk and egg yolk diluent for 5 days storage is 43.33a ± 6.05, 56.50a ± 3.08 and 28.33a ± 2.80. The conclusion of this study is the addition of 0.15% green tea extract in a skim milk and egg yolk diluent can maintain the quality of sperm for up to 5 days of storage at cold temperatures.
PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER KUNING TELUR SITRAT TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN Itrah Khoirunnisa; Suherni Susilowati; Lilik Maslachah; Tri Wahyu Suprayogi; Rochmah Kurnijasanti; Hermin Ratnani
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.347 KB) | DOI: 10.20473/ovz.v8i2.2019.127-131

Abstract

The purpose of this research was determine the effect and the best concentration of greentea extract in egg yolk citrate diluent for quality Sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups : egg yolk citrate diluent, 0,05 % green tea extract in egg yolk citrate diluent, 0,1 % green tea extract in egg yolk citrate diluent and 0,15 % green tea extract in egg yolk citrate diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after diluent. The data obtained was analyzed with the analysis of variance (ANOVA), followed by multiple range test duncan. The result showed that the higest precentage of motility, viability and intact plasma membran were obtained from 0,05 % green tea extract in egg yolk citrate diluent for 1 day of storage which were 85.83 ± 3.76, 91.16 ± 2.13 and 66.83 ± 6.49. The lowest precentage of motility, viability and intact plasma membrane were obtained from egg yolk citrate diluent for 5 day of storage which were 26.66 ± 6.05, 49.16 ± 6.43 and 26.66 ± 4.32. Conclution of this research was the addition of 0,05 % green tea extract can be used as a diluent could maintain the precentage of spermatozoa quality until 5 days after diluent.