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Perbandingan Fungsi Paru Juru Parkir Basement dengan Juru Parkir Ruang Terbuka di Kota Bandung Trissekti, Galih; Kusmiati, Mia; Budiman, Budiman
Global Medical & Health Communication (GMHC) Vol 2, No 2 (2014)
Publisher : Fakultas Kedokteran Universitas Islam Bandung

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Abstract

Abstrak Polusi udara bertanggung jawab atas 3,1 juta kematian seluruh dunia setiap tahunnya. Efek buruk dari polusi udara berdasarkan penelitian pada tiga lokasi berbeda di Beijing, Cina tahun 1986, menyatakan bahwa peningkatan konsentrasi polusi udara sebesar 1 mikrogram/m3 mampu menurunkan forced expiratory volume in one second (FEV1) sebesar 35,6 mL. Populasi yang berisiko mengalami masalah pernapasan akibat terpapar asap kendaraan yang dapat terhirup setiap waktu ini secara jangka panjang, salah satunya adalah juru parkir. Tujuan penelitian ini untuk mengetahui perbandingan fungsi paru juru parkir basement dengan juru parkir ruang terbuka di Kota Bandung periode Januari–Juni2014. Desain penelitian bersifat analitik kuantitatif dengan rancangan potong lintang terhadap masig-masing 33 subjek yang berprofesi sebagai juru parkir basement dan juru parkir ruang terbuka. Terlebih dahulu dilakukan pengukuran data karakteristik fisik berupa usia (tahun) dan IMT (kg/m2), selanjutnya dilakukan pengukuran fungsi paru menggunakan parameter FEV1, forced vital capacity (FVC), dan FEV1/FVC dengan spirometri, kemudian dibandingkan antara kedua kelompok juru parkir. Hasil penelitian menunjukkan bahwa nilai median FEV1 juru parkir basement (3.300 mL, range 2.600–4.400 mL) dan juru parkir ruang terbuka (3.000 mL, range 2.300–3.800 mL) dengan nilai p=0,011. Nilai FVC rata-rata juru parkir basement (3.587,88 ± 470,21 mL) dan juru parkir ruang terbuka (3.287,88 ± 478,77 mL) dengan nilai p=0,013. Nilai median FEV1/FVC juru parkir basement (0,94; range 0,79–0,98) dan juru parkir ruang terbuka (0,92; range 0,77–0,97) dengan nilai p=0,016.Simpulan hasil penelitian menunjukkan fungsi paru yang digambarkan dengan FEV1, FVC, dan FEV1/FVC pada juru parkir basement lebih baik daripada juru parkir ruang terbuka.   Kata kunci: Fungsi paru, juru parkir basement, juru parkir ruang terbuka The Comparison of Lung Function between Basement Parking and Street Parking Attendants in Bandung City Abstract Air pollutions responsible for 3.1 milion death in the world every years. The bad effect from it according to research in three different places of Beijing (1986) reveal that enhancement 1 microgram/m3 of air pollution concentration can cause reduction forced expiratory volume in one second (FEV1) about 35.6mL. Population with high risk to have respiratory disorder as consequence exposed to vehicle’s smoke that can inhaled anytime and long-term, one of which is parking attendants. This research is therefore conducted to obtain comparison of lung function between basement parking attendants and street parking attendants in Bandung city period January–June 2014. This research design is quantitative analysis with cross sectional method towards each 33 subjects that worked as basement parking attendants and street parking attendants. The demography characteristic such as age (years old) and BMI (kg/m2). Further performed test of lung function with parameters: FEV1, forced vital capacity (FVC), and FEV1/FVC by spirometer, furthermore compared between the two groups of parking attendants. The research result showed that FEV1 median score of basement parking attendants (3,300 mL, range 2,600–4,400 mL) and street parking attendants (3,000 mL, range 2,300–3,800 mL) with p=0.011. The FVC average score of basement parking attendants (3,587.88 ± 470.21 mL) and street parking attendants (3,287.88 ± 478.77 mL) with p=0.013. The FEV1/FVC median score of basement parking attendants (0.94; range 0.79–0.98) and street parking attendants (0.92; range 0.77–0.97) with p=0.016. In conclusion lung function described by FEV1, FVC, and FEV1/FVC in basement parking attendants are better than street parking attendants, with all score are significant.   Key words: Basement parking attendant, lung function, street parking attendants 
IDENTIFIKASI AKTINOMISETES SEDIMEN AIR TAWAR MAMASA, SULAWESI BARAT DAN AKTIVITASNYA SEBAGAI ANTIBAKTERI DAN PELARUT FOSFAT Putri, Ade Lia; Lisdiyanti, Puspita; Kusmiati, Mia
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 2 (2018): December 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (542.935 KB) | DOI: 10.29122/jbbi.v5i2.2953

Abstract

Identification of Actinomycetes in Freshwater Sediments from Mamasa, West Sulawesi and Their Antibacterial and Phosphate Solubilizing ActivitiesABSTRACTA large number of actinomycetes that have been isolated and screened were obtained from soil and marine samples. Consequently, the possibility of isolating novel Actinomycetes and secondary metabolites compounds strains from soil and marine samples have limited. Exploration of actinomycetes from freshwater sediment is rare. In this study, 30 isolates of Actinomycetes from freshwater sediments in Mamasa District, West Sulawesi were isolated, identified, and screened for their antibacterial and phosphate solubilizing activity. Actinomycetes were isolated by serial dilution method and were identified based on morphological and 16S rRNA gene sequence. Antibiotic activity was screened using the agar plug diffusion method, while soluble phosphate ability was observed by clear zone ratio in PKA medium. Most of the isolates belong to the genus Streptomyces (80%). Out of 30 isolates, 56.6% showed antibacterial activity and 36.6% had potential as solubilizing phosphate which belong to genus Streptomyces, Actinomadura, and Kitasatospora.Keywords: 16S rRNA, Actinomycetes, antibacterial, freshwater sediment, phosphate solubilizing ABSTRAKSebagian besar aktinomisetes yang telah diisolasi dan dilakukan penapisan metabolit sekundernya berasal dari sampel tanah dan laut. Konsekuensinya, kesempatan untuk menemukan aktinomisetes jenis baru maupun yang menghasilkan metabolit sekunder baru dari tanah dan laut semakin berkurang. Eksplorasi aktinomisetes dari lingkungan lain seperti sedimen air tawar jarang dilakukan. Pada penelitian ini, 30 isolat aktinomisetes yang diisolasi dari sedimen air tawar di Kabupaten Mamasa, Sulawesi Barat, telah diidentifikasi dan dilakukan penapisan antibakteri dan kemampuan isolat dalam melarutkan fosfat. Aktinomisetes diisolasi dengan metode pengenceran secara langsung dan selanjutnya diidentifikasi secara morfologi dan molekular berdasarkan gen 16S rRNA. Metode yang digunakan dalam penapisan aktivitas antibakteri adalah agar plug diffusion method, sedangkan kemampuan aktinomisetes dalam melarutkan fosfat diuji dengan cara menumbuhkan isolat pada media PKA. Isolat yang paling banyak diisolasi termasuk ke dalam marga Streptomyces (80%). Dari 30 isolat, 56,6% isolat menunjukkan adanya aktivitas antibakteri dan 36,6% dari isolat berpotensi sebagai pelarut fosfat, yang termasuk ke dalam marga Streptomyces, Actinomadura, dan Kitasatospora.Kata Kunci: 16S rRNA, Aktinomisetes, sedimen air tawar, antibakteri, pelarut fosfat
ISOLATION AND IDENTIFICATION OF ACTINOMYCETES ASSOCIATED WITH MOSS ON THE SURFACE OF THE BOROBUDUR TEMPLE STONE Putri, Ade Lia; Purbani, Debora Christin; Kanti, Atit; Kusmiati, Mia; Habibi, Moh
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.20334

Abstract

Mosses growing on the surface of the Borobudur Temple will affect the aesthetic value of the temple. Interaction between moss and actinomycetes may trigger the growth of moss that can cause an increase in biodeterioration of stone. The purpose of this study was to isolate and identify the actinomycetes associated with moss on the surface of decayed stone of Borobudur Temple as well to assess their ability for phosphate solubilizing. Actinomycetes were isolated using serial dilution method and were identified based on 16S rRNA gene sequences. A total of 37 actinomycetes were isolated from three sampling sites. The isolates found belong to five genera (Gordonia, Microbacterium, Micromonospora, Nocardia, and Streptomyces) and distributed among four families (Microbacteriaceae, Micromonosporaceae, Nocardiaceae, and Streptomycetaceae). Isolates of actinomycetes composed of 19 Streptomyces Group and 18 Rare Actinomycetes Group. Nineteen isolates (51.35%) were identified as genus Streptomyces. Seventeen isolates (45.94%) showed abilities to release soluble phosphate and most of the isolates belong to the genus Streptomyces. The isolates have been collected will be deposited to Indonesian Culture Collection (InaCC) to enrich the collection of actinomycetes from ancient stone in Indonesia and will be used as a source of reference material research, taxonomic, or as source for further study.
PENGARUH PERMAINAN TRADISIONAL ANJANG-ANJANGAN TERHADAP KARAKTER TANGGUNG JAWAB ANAK USIA DINI Kusmiati, Mia; kurniati, Euis; Aryaprasetya, I Gusti Komang
Edukids: Jurnal Pertumbuhan, Perkembangan, dan Pendidikan Anak Usia Dini Vol 13, No 2 (2016)
Publisher : Prodi PGPAUD

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.795 KB) | DOI: 10.17509/edukid.v13i2.16921

Abstract

The  Influence  Of  Anjang-anjangan  Traditional  Game  On  Young Children’s  Character  Trait  Of  Responsibility.  The  research  intends  to  find  the influence  of  anjang-anjangan  traditional  game  on  young  children’s  character  trait  of responsibility. It adopted quasi-experimental method with nonequivalent control group design.  The  research  was  conducted  to  Group  B  of  State  Kindergarten  Centeh, Bandung City, with a number of 14 and 17 children as the sample for the experimental and control groups, respectively. Data were collected using a research instrument in the form of observation guidelines. The results of the pretest show that 25% of the children in the experimental group had shown an emerging character trait of responsibility and the  rest  75%  had  not;  meanwhile,  36%  of  the  children  in  the  control  group  had demonstrated  an  emerging  character  trait  of  responsibility,  and  the  rest  64%  had  not. After  treatment,  results  of  the  posttest  show  that  the  number  of  children  in  the experimental  group  showing  the  emergence  of  responsibility  character trait  was 73%, and those who did not 27%, whereas in the control group the percentages of children who  showed  and  did  not  show  emerging  responsibility  character  trait  were  61%  and 39%,  respectively.  With  the  result  of  t-test  showing  that  p  (0.038)    0.05,  Ha  was accepted,  meaning  that  there  was  a  significant  difference  in  the  character  trait  of responsibility between the experimental and control groups. Based these findings, it is recommended  that  teachers  of  young  children  employ  anjang-anjangan  traditional game  as  one  of  the  alternative  methods  to  develop  young  children’s  responsibility character trait.    Abstrak: Pengaruh Permainan Tradisional Anjang-anjangan Terhadap Karakter Tanggung  Jawab  Anak  Usia  Dini.  Penelitian  ini  bertujuan  untuk  mengetahui pengaruh  permainan  tradisional  anjang-anjangan  terhadap  karakter  tanggung  jawab anak  usia  dini.  Metode  penelitian  yang  digunakan  adalah  kuasi  eksperimen  dengan desain  penelitian  nonequivalent  control  group  desain.  Penelitian  ini  dilakukan  pada kelompok  B  di  Taman  Kanak-kanak  Negeri  Centeh  Kota  Bandung,  dengan  jumlah sampel kelompok eksperimen sebanyak 14 orang dan kontrol sebanyak 17 orang. Data penelitian  diperoleh  menggunakan  instrumen  penenlitian  berupa  pedoman  observasi. Berdasarkan  hasil  penelitian  bahwa  data  pretest  pada  kelompok  eksperimen menunjukkan anak yang muncul karakter tanggung jawabnya sebanyak 25% dan yang belum muncul sebanyak 75%, pada kelompok kontrol  menunjukkan anak yang muncul karakter  tanggung  jawabnya  sebanyak  36%  dan  yang  belum  muncul  sebanyak  64%. Setelah  dilakukan  treatment  hasil  akhir  pada  data  posttest  kelompok  eksperimen menunjukkan anak yang muncul karakter tanggung jawabnya sebanyak 73% dan yang belum muncul sebanyak 27%, pada kelompok kontrol menunjukkan anak yang muncul karakter  tanggung  jawabnya  sebanyak  49%  dan  yang  belum  muncul  sebanyak  51%, dengan  perolehan  akhir    uji  t  menunjukkan  P  (0,038)    0,05  maka  Ha  diterima,  yang artinya  terdapat  perbedaan  yang  signifikan  karakter  tanggung  jawab  antara  kelompok eksperimen  dengan  kelompok  kontrol.  Berdasarkan  hasil  penelitian  tersebut  peneliti merekomendasikan  kepada  pendidik  anak  usia  dini  supaya  permainan  tradisional anjang-anjangan  dijadikan  sebagai  metode  alternative  mengembangkan  karakter tanggung jawab pada anak usia dini.   
IDENTIFIKASI AKTINOMISETES SEDIMEN AIR TAWAR MAMASA, SULAWESI BARAT DAN AKTIVITASNYA SEBAGAI ANTIBAKTERI DAN PELARUT FOSFAT Putri, Ade Lia; Lisdiyanti, Puspita; Kusmiati, Mia
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (542.935 KB) | DOI: 10.29122/jbbi.v5i2.2953

Abstract

Identification of Actinomycetes in Freshwater Sediments from Mamasa, West Sulawesi and Their Antibacterial and Phosphate Solubilizing ActivitiesABSTRACTA large number of actinomycetes that have been isolated and screened were obtained from soil and marine samples. Consequently, the possibility of isolating novel Actinomycetes and secondary metabolites compounds strains from soil and marine samples have limited. Exploration of actinomycetes from freshwater sediment is rare. In this study, 30 isolates of Actinomycetes from freshwater sediments in Mamasa District, West Sulawesi were isolated, identified, and screened for their antibacterial and phosphate solubilizing activity. Actinomycetes were isolated by serial dilution method and were identified based on morphological and 16S rRNA gene sequence. Antibiotic activity was screened using the agar plug diffusion method, while soluble phosphate ability was observed by clear zone ratio in PKA medium. Most of the isolates belong to the genus Streptomyces (80%). Out of 30 isolates, 56.6% showed antibacterial activity and 36.6% had potential as solubilizing phosphate which belong to genus Streptomyces, Actinomadura, and Kitasatospora.Keywords: 16S rRNA, Actinomycetes, antibacterial, freshwater sediment, phosphate solubilizing ABSTRAKSebagian besar aktinomisetes yang telah diisolasi dan dilakukan penapisan metabolit sekundernya berasal dari sampel tanah dan laut. Konsekuensinya, kesempatan untuk menemukan aktinomisetes jenis baru maupun yang menghasilkan metabolit sekunder baru dari tanah dan laut semakin berkurang. Eksplorasi aktinomisetes dari lingkungan lain seperti sedimen air tawar jarang dilakukan. Pada penelitian ini, 30 isolat aktinomisetes yang diisolasi dari sedimen air tawar di Kabupaten Mamasa, Sulawesi Barat, telah diidentifikasi dan dilakukan penapisan antibakteri dan kemampuan isolat dalam melarutkan fosfat. Aktinomisetes diisolasi dengan metode pengenceran secara langsung dan selanjutnya diidentifikasi secara morfologi dan molekular berdasarkan gen 16S rRNA. Metode yang digunakan dalam penapisan aktivitas antibakteri adalah agar plug diffusion method, sedangkan kemampuan aktinomisetes dalam melarutkan fosfat diuji dengan cara menumbuhkan isolat pada media PKA. Isolat yang paling banyak diisolasi termasuk ke dalam marga Streptomyces (80%). Dari 30 isolat, 56,6% isolat menunjukkan adanya aktivitas antibakteri dan 36,6% dari isolat berpotensi sebagai pelarut fosfat, yang termasuk ke dalam marga Streptomyces, Actinomadura, dan Kitasatospora.Kata Kunci: 16S rRNA, Aktinomisetes, sedimen air tawar, antibakteri, pelarut fosfat
Santri trained troops: Making new normal kits and training for the COVID-19 task force Damayanti, Meta Maulida; Yuniarti, Yuniarti; Rachmawati, Meike; Kusmiati, Mia
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol 7, No 1 (2022): February 2022
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v7i1.6020

Abstract

Since the COVID-19 pandemic first emerged in December 2019 and was confirmed in Indonesia in March 2020, the Indonesian government has imposed a strict lockdown. The number of confirmed cases is increasing all the time. Efforts to prevent transmission must be carried out immediately, including improving health protocols. The activity aims to create trained troops Santri, by providing insight into COVID-19 which consists of training to implement health protocols and soft skills for making new normal kits, namely hand sanitizers and cloth masks. The activities were carried out at the Manarul Huda Islamic Boarding School, Bandung to 31 Santri. The new normal kit manufacturing training is accompanied by an expert. The training of Santri produces cadres with a level of knowledge about COVID-19 most highly with a fair category (51,61%). Based on the percentage of the group, age17 years, male gender, and education level graduated from school have a higher level of good knowledge than other groups. This service activity produces trained troops Santri equipped with knowledge about COVID-19 particularly good health protocols, has soft skills to make hand sanitizers and cloth masks. Towards they can produce a new normal kit and create a COVID-free Islamic boarding school area.