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All Journal Indonesian Journal of Pharmaceutical Science and Technology Majalah Obat Tradisional Indonesian Journal of Natural Pigments Media Farmasi Indonesia
Lia Kusmita
Sekolah Tinggi Ilmu Farmasi Yayasan Pharmasi
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UJI AKTIVITAS ANTIOKSIDAN DAN PENENTUAN KANDUNGAN ANTOSIANIN TOTAL KULIT BUAH MANGGIS (Garcinia mangostana L) Kusmita, Lia; Wulansari, Endang Dwi; Supiyanti, Wiwin
Majalah Obat Tradisional Vol 15, No 2 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.473 KB) | DOI: 10.14499/mot-TradMedJ15iss2pp%p

Abstract

Buah manggis dengan nama latin Garcinia mangostana L. mendapat julukan Queen of tropical fruit (Ratunya Buah-buahan Tropik). Kulit buah manggis berwarna ungu kehitaman. Diduga kulit buah manggis mengandung senyawa antosianin. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan ekstrak kulit buah manggis dan kandungan antosianin total dalam kulit buah manggis. Obyek penelitian adalah kulit buah manggis yang sudah layak untuk dikonsumsi. Kulit buah manggis dihaluskan kemudian direndam dengan pelarut metanol yang mengandung HCl 1%. Dilakukan uji aktivitas antioksidan menggunakan metode DPPH dan penentuan kandungan antosianin total dalam kulit buah manggis menggunakan metode perbedaan pH. Uji aktivitas antioksidan ekstrak kulit buah manggis meliputi uji aktivitas antioksidan secara kualitatif berupa lempeng KLT ekstrak kulit buah manggis disemprotkan penampak bercak DPPH 0,1 mM menghasilkan bercak kuning pucat dengan latar belakang ungu. Uji aktivitas antioksidan secara kuantitatif berdasarkan parameter EC50 menggunakan metode DPPH dengan pembanding vitamin C. Diperoleh nilai EC50 sebesar 8,5539 μg/mL dan nilai EC50 vitamin C adalah 3,3676 μg/mL. Rata-rata kandungan antosianin total dalam kulit buah manggis adalah 59,3 mg dalam 100 gram kulit buah manggis. 
Uji Aktivitas Antibakteri Pigmen Karotenoid Dari Bakteri Simbion Karang Lunak Sarcophyton sp. Terhadap Pertumbuhan Bakteri Patogen Staphylococcus aureus ATCC 25923 Wiguna, Awang Surya; Kusmita, Lia; Radjasa, Ocky Karna
Indonesian Journal of Pharmaceutical Science and Technology Vol 3, No 3 (2016)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (692.307 KB) | DOI: 10.15416/ijpst.v3i3.9176

Abstract

Mikroba penghasil antibiotik dapat berupa fungi maupun bakteri yang bersimbiosis pada organisme lain. Pigmen karotenoid dari bakteri simbion karang lunak merupakan mikroorganisme yang berpotensi sebagai antibakteri. Tujuan penelitian ini untuk mengetahui aktivitas antibakteri ekstrak pigmen karotenoid dari bakteri simbion karang lunak Sarcophyton sp. terhadap pertumbuhan bakteri patogen Staphylococcus aureus ATCC 25923 serta mengetahui perbedaan aktivitas antibakteri ekstrak pigmen karotenoid pada konsentrasi 0,5 %, 0,75 %, dan 1 % dengan metode sumuran. Bakteri yang menghasilkan pigmen karotenoid dibiakkan dan diidentifikasi dengan mengisolasi DNA untuk dilakukan PCR dan mensequen hasilnya. Hasil penelitian aktivitas antibakteri dengan rata – rata diameter zona hambat pada konsentrasi 0,5 % sebesar 0,678 cm, konsentrasi 0,75 % sebesar 0,978 cm, konsentrasi 1 % sebesar 1,416 cm serta diameter zona hambat kontrol positif amoksisilin trihidrat sebesar 1,875 cm.
EFEK ANALGETIK TEPUNG UMBI BIDARA UPAS (MERREMIA MAMMOSA (LOUR) HALL. F.) PADA MENCIT JANTAN Puspitaningrum, Ika; Kusmita, Lia
Media Farmasi Indonesia Vol 9 No 2 (2014): Media Farmasi Indonesia
Publisher : STIFAR "YAYASAN PHARMASI SEMARANG"

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Abstract

Abstract                Analgesic is a compound that can reduce or eliminate pain without losing consciousness. Bidara upas (Merremia mammosa (Lour) Hall. F.) is one of the flora that part edible tubers and useful for the treatment of, among others, anti-inflammatory and analgesic. The content of flavonoids and alkaloids in the tubers bidara upas could be expected to have an effect as an analgesic.                This study aims to determine the analgesic effect bidara upas tuber starch (BUTS) and starch tubers effective dose bidara upas as an analgesic. This study was an experimental study with random sampling technique (random sampling).                Testing of analgesic effect (BUTS) using 25 male mice Swiss strain which is divided into 5 groups. Group I, II, and III as a dose of 1, 2 and 3 are a group (BUTS) giving a dose of 1,5; 3; and 6 g/kgBB, IV as a negative control group is a group of distilled water provision, as well as the positive control group V is a group of administration of paracetamol dose 91 mg/kgBB. All treatments are given orally. 5-minute intervals after treatment, all mice were given painful stimuli in the form of a sterile solution of glacial acetic acid 1% v / v with a dose of 300 mg/kgBB and observed stretching that arise every interval of 5 minutes for 60 minutes. Total cumulative stretching all treatment groups were analyzed statistically with SPSS 16 with a 95% confidence level. It also calculated percent analgesic power all treatment groups.             The results obtained are significant differences between the groups with a negative control group tuber flour upas bidara three doses. This proves tuber flour bidara upas have analgesic effect. In addition, the results obtained are significant differences between the positive control group by group tuber flour upas bidara three doses. Tuber bidara upas starch total cumulative dose has smaller stretching and % power analgesic greater than the paracetamol group. Based on these results, the effective dose tuber flour bidara upas as analgesic of 1,5 g/kgBB of mice.
UJI AKTIVITAS GEL EKSTRAK ETANOL KULIT BUAH MANGGIS (GARCINIA MANGOSTANA L.) SEBAGAI PENYEMBUH LUKA BAKAR PADA KULIT PUNGGUNG KELINCI -, Mutmainah; Kusmita, Lia; Puspitaningrum, Ika
Media Farmasi Indonesia Vol 9 No 1 (2014): Media Farmasi Indonesia
Publisher : STIFAR "YAYASAN PHARMASI SEMARANG"

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Abstract

ABSTRACT Mangosteen is a skin shells discarded by consumers or may be called by the waste of agricultural products. Mangosteen rind  contain xanthone, flavonoids, saponins and tannins that are thought to be potentially heal burn.   The air of this study was to determine the effect of ethanolic extract of mangosteen rind with a concentration of 2,5%, 5%, 10 % of ethanol extract of mangosteen rind  in the preparation of the gel on the healing of burns on the back of rabbits. The research is conducted using male rabbit which was divided into five groups, namely the group of positive control, negative control, mangosteen rind extract gel ethanol concentration of 2,5%, 5%, 10 %. Induction plates use iron burns with size of 2 cm is heated at a temperature of 100°C for 5 seconds, these gels are done every day until wound healing as seen from the time required for diameter shrink to the perfect closing wound.  The results of pharmacological tests of statistical tests with increased concentrations of the extract showed a significant difference in each group viewed from a long healing (p <0.05). Physical characteristics of the test results showed that the concentration of the ethanol extract of mangosteen rind in a row with a concentration of 2,5%, 5%, 10% were able to increase the viscosity, pH, adhesion and lower dispersive power.
INDEKS GLIKEMIK DAN ANALISIS MAKRONUTRIEN TEPUNG UMBI KIMPUL (XANTHOSOMA VIOLACEUM SCHOTT.) SEBAGAI ANTIDIABETES MELITUS TIPE II Puspitaningrum, Ika; Kusmita, Lia; -, Mutmainah
Media Farmasi Indonesia Vol 9 No 1 (2014): Media Farmasi Indonesia
Publisher : STIFAR "YAYASAN PHARMASI SEMARANG"

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Abstract

Abstrak Diabetes mellitus tipe 2 atau diabetes mellitus tidak tergantung insulin (DMTTI) merupakan tipe diabetes melitus yang terjadi karena menurunnya kepekaan jaringan pada insulin. Tepung umbi kimpul (Xanthosoma violaceum Schott.) telah terbukti sebagai antidiabetes mellitus tipe II. Penelitian ini bertujuan untuk mengetahui nilai indeks glikemik tepung umbi kimpul. Selain itu, penelitian ini juga bertujuan untuk mengetahui kadar makronutrien dalam tepung umbi kimpul yang meliputi karbohidrat, protein, lemak dan serat. Pengukuran nilai indeks glikemik menggunakan hewan uji tikus jantan galur Wistar usia 2-3 bulan sebanyak 15 ekor. Hewan uji dibagi menjadi 3 kelompok perlakuan yaitu pemberian glukosa standar dan suspensi tepung umbi kimpul dalam CMC-Na 0,1 % dosis 2g/kg berat badan serta CMC Na 0,1% secara oral. Selanjutnya sampling serum darah tikus dilakukan pada jam ke-0, 1 dan 2 setelah pemberian bahan uji. Penetapan kadar glukosa darah menggunakan metode GOD-PAP. Kadar glukosa darah yang diperoleh selanjutnya dihitung nilai indeks glikemiknya (IG). Nilai IG suweg dapat dilakukan dengan cara membandingkan nilai Area Under Curve (AUC) glukosa darah individu setelah pemberian sampel dengan nilai AUC glukosa standar yang bernilai IG 100. Perhitungan AUC mengikuti rumus trapesium yang terbentuk di daerah bawah kurva antara waktu (jam) dengan kadar glukosa (mg/dL). Hasil penelitian menunjukkan nilai Indeks Glikemik tepung umbi kimpul sebesar 0,29. Nilai IG tepung umbi kimpul masuk klasifikasi IG yang sangat rendah. Analisa makronutrien menunjukkan bahwa tepung umbi kimpul mengandung karbohidrat sebesar 52,2%, protein sebesar 3,07%, lemak sebesar 0,44% dan serat sebesar 3,00%.
UJI AKTIVITAS PENGANGKAPAN RADIKAL 2,2-DIFENIL-1-PIKRILHIDRAZIL (DPPH) PIGMENT BAKTERIAL SIMBION DARI SARGASSUM POLYCYTRUM Kusmita, Lia; Nugraheni, Sri Achadi; Nuryadi, Handung
Media Farmasi Indonesia Vol 8 No 2 (2013): Media Farmasi Indonesia
Publisher : STIFAR "YAYASAN PHARMASI SEMARANG"

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Abstract

Carotenoids are pigments that are found in plants, animals, microorganisms and humans. The pigment has many functions, one of which is as an antioxidant. There have been many studies of carotenoids in plants, but the types of carotenoids in bacteria is very rarely observed when the potential is not lost. The ability of marine bacteria is well known to produce carotenoids. Bacterial symbionts associated with Sargassum polycytrum can produce neoxanthin. This research will test the antioxidant activity by DPPH method on bacterial symbionts pigment extracts as compared with extracts of S. polycytrum. Based on the results obtained IC50 of 3.01 ± 2350 ppm was obtain for extract pigments S. polycytrum, 645 ± 2.11 ppm for pigment extract bacterial symbionts, and 564 ± 2.26 ppm for ?-carotene as a comparison.
Activity Antioxidant on Pigments of Bacterial Symbionts of Soft Coral From Jepara Sea Masduqi, Ahmad Fuad; Franyoto, Yuvianto Dwi; Kusmita, Lia; Muchlisin, Sakti; Widyananto, Prasetyo Abi; Sulistyani, Sulistyani; Wijayanti, Diah Permata
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.43

Abstract

Soft corals have been known to produce secondary metabolites, some of which may have anticancer, antifouling, antibacterial and antioxidants activity. It has been suggested that natural products from marine invertebrates have striking similarities to metabolites of their association microorganisms. Symbiont bacteria on soft coral can produce bioactive compounds that play an important role in chemical ecology and as a marine natural product. Marine bacteria associated with soft coral collected from Jepara were successfully isolated on medium ZoBell 2216E and screened to synthesize the pigment. This approach has allowed the use of this organism as an environmentally friendly alternative source of new natural pigment. This study found 25 bacterial isolates from 6 types of soft coral. Out of 25 bacterial isolates, only 3 bacterium, positively contains pigments. Four isolates, PCl 1, PS2 1, and PSa 2. Pigments analysis with UV spectrophotometric method showed the wavelength of pigments were in the range 300-600 nm. Genomic DNA was isolated from these colonies and nested PCR of the DNA was performed to amplify the 16S rDNA. Antioxidant activity was tested with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. From the results of molecular identification by 16S rDNA method, it was shown that bacterium PCl 1, PS2 1, and PSa 2 was closely related to Pseudomonas stutzeri, Ponticoccus gilvus, Bacillus marisflavi with 99%, 99and 98% homology value. Antioxidant activity is as follows: PCl 1>PS2 1>PSA 2.
Co-Authors Ahmad Fuad Masduqi Awang Surya Wiguna, Awang Surya Diah Permata Wijayanti Endang Dwi Wulansari Franyoto, Yuvianto Dwi Handung Nuryadi Ika Puspitaningrum Muchlisin, Sakti Mutmainah -, Mutmainah Ocky Karna Radjasa Sri Achadi Nugraheni Sulistyani Sulistyani Widyananto, Prasetyo Abi Wiwin Supiyanti, Wiwin