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OBTAINING Artemisia cina POLYPLOIDY THROUGH PLANT GROWTH REGULATOR TREATMENT IN SHOOT CULTURE Herawati, Maria Marina; Pudjihartati, Endang; Pramono, Suwijiyo; Sulistyaningsih, Endang; Purwantoro, Aziz
AGRIVITA, Journal of Agricultural Science Vol 37, No 2 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Artemisia cina is a medicinal plant species which produces bioactive compound potential to anti-tumor, antifungal and antibacterial medicines. This study was aimed to obtain A. cina polyploid plants through a treatment of growth regulators in shoot culture. The shoot were treated in 1; 15; 2; and 3 mg L-1 of 2,4-dichloro phenoxy acetic acid (2,4-D) combined with 1; 1.5; 2; 3 mg L-1 of Benzyladenine (BA) for 28 days. Chromosome analysis showed that the highest polyploidy percentage, 23%, was reached in the treatment of 2 mg L-1 of 2,4-D combined with 1 of BA mg L-1. The polyploidy level varied, comprising 2n=3x, 2n=4x, 2n=5x, 2n=6x, with the highest polyploidy level percentage, 28.57%, and it was attained in the tetraploid (2n=4x). Polyploid plants had larger leaves area, larger stomatal size, and higher chlorophyll content than diploid plants. However stomatal density of polyploidy plants was lower than that of in diploid plants.Keywords: 2,4-D, Artemisia cina, BA, polyploidy
ISOLASI, IDENTIFIKASI DAN ANTAGONISME IN VITRO ISOLAT TRICHODERMA SPP. ASAL KEBUN KARET BLIMBING, PEKALONGAN, JAWA TENGAH Berlian, Intan; Anarqi, Sindu; Pudjihartati, Endang
Jurnal Penelitian Karet JPK : Volume 34, Nomor 2, Tahun 2016
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v34i2.231

Abstract

Rigidoporus microporus merupakan patogen penyebab penyakit jamur akar putih (JAP) yang sangat merugikan perkebunan karet di Kebun Blimbing, Pekalongan, Jawa Tengah. Tindakan pengendalian secara preventif, kuratif dengan cara kultur teknis dan kimiawi sudah dilakukan namun belum dapat mengendalikannya. Penelitian ini bertujuan untuk mendapatkan isolat lokal perkebunan karet di Kebun Blimbing yang bersifat antagonis terhadap JAP. Penelitian terdiri dari: (1) isolasi, (2) identifikasi, dan (3) uji antagonisme dengan metode dual culture. Pengujian menggunakan rancangan acak lengkap. Penelitian ini berhasil mendapatkan sebelas isolat Trichoderma spp. dari rhizosfer tanaman karet di Kebun Blimbing. Sebelas isolat tersebut yaitu Trichoderma spp. dari Blok Sikaum TBM 1 (P1), Blok Sikaum TBM 2 (P2), Blok Sikaum TM 1 (P3), Blok Sikaum TM 2 (P4), Blok Sari TM 1 (P5), Blok Sari TM 2 (P6), Blok Sitirejo TBM 1 (P7), Blok Sitirejo TBM 2 (P8), Blok Semurup TM 1 (P9), Blok Semurup TM 2 (P10), Blok Semurup TM 3 (P11). Semua isolat Trichoderma spp. tersebut bersifat antagonis terhadap pertumbuhan R. microporus. Persentase daya hambat Trichoderma spp. terhadap R. microporus berkisar antara 36,56% sampai 69,66%. Penghambatan paling tinggi (69,66%) diamati pada isolat P9 dan terendah (36,56%) pada isolat P3.
Bio-Priming Benih Kedelai (Glycine Max (L.) Merrill) untuk Meningkatkan Mutu Perkecambahan Livia Trihanni Hasan, Theresa Dwi Kurnia Endang Pudjihartati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 1, No 2 (2016): June 2016
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.689 KB) | DOI: 10.24002/biota.v1i2.992

Abstract

Sifat benih kedelai dengan kandungan protein dan lemak tinggi menjadi penyebab benih kedelai cepat mengalami deteriorasi atau penurunan mutu benih. Salah satu cara untuk meningkatkan mutu perkecambahan adalah dengan perlakuan pemeraman atau bio-priming. Tujuan penelitian ini adalah mengetahui pengaruh bio-priming dalam meningkatkan mutu perkecambahan. Penelitian ini menggunakan benih kedelai kuning varietas Grobogan yang sudah disimpan selama tiga bulan. Rancangan percobaan menggunakan Rancangan Acak Kelompok (RAK) dan data yang diperoleh dianalisis sidik ragam dengan uji Beda Nyata Jujur (BNJ) taraf signifikansi 5%. Perlakuan dalam penelitian ini adalah: 1) Kontrol (K), 2) Priming (M) perlakuan matriks priming dengan memasukkan benih kedelai ke dalam media berupa arang sekam lembab dengan perbandingan benih : arang sekam : air = 3 : 10 : 3 (b/b/v), 3) Bio-priming menggunakan EM-4 (E) dengan cara merendam benih dalam larutan EM-4 0.3% selama satu jam, 4) Bio-priming menggunakan Trichoderma harzianum (T) yaitu priming benih dengan 75 g Trichoderma harzianum selama satu hari, dan 5) Bio-priming menggunakan EM-4 + Trichoderma harzianum (ET). Hasil penelitian menunjukkan bahwa bio-priming dengan menggunakan EM-4 mampu memperbaiki viabilitas, vigor dan pertumbuhan kecambah kedelai, sedangkan bio-priming menggunakan Trichoderma harzianum tidak menunjukkan perbedaan nyata pada semua variabel pengamatan dibandingkan kontrol. Perlakuan bio-priming menggunakan kombinasi EM-4 dan Trichoderma harzianum cenderung menurunkan mutu perkecambahan kedelai dibandingkan kontrol.
Embriogenesis Somatik dari Eksplan Benih Gandum Tropis (Triticum aestivum L.) Pudjihartati , Endang; Marina Herawati , Maria
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 17, No 2 (2012): June 2012
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (24.699 KB) | DOI: 10.24002/biota.v17i2.136

Abstract

Embriogenesis somatik dalam penelitian ini menggunakan eksplan embrio dewasa (benih) gandum tropis varietas Dewata dan galur R-036 dan R-040 koleksi Pusat Studi Gandum Fakultas Pertanian dan Bisnis UKSW, meliputi tahap induksi dan proliferasi. Penelitian ini bertujuan mendapatkan genotipe gandum tropis yang mudah di kultur secara in vitro dan medium yang baik dalam rangka memperoleh Embrio Somatik (ES). Pada tahap induksi, benih gandum dikultur pada empat konsentrasi 2,4-d (2; 2,5; 3 dan 3,5 ppm). Pada tahap proliferasi diteliti empat komposisi media yang mengandung 2,5 dan 3 ppm 2,4-d dengan dan tanpa 0,5 ppm BAP. Hasil penelitian menunjukkan bahwa jumlah ES dan skor ES primer relatif tertinggi dari varietas Dewata pada 3 ppm 2,4-d, galur R-040 pada 2,5 ppm 2,4-d, dan galur R-036 pada 2 ppm 2,4-d. Eksplan gandum galur R-036 menghasilkan jumlah ES dan skor ES primer relatif tertinggi dibandingkan genotipe lain. Mutu benih yang digunakan mempengaruhi respons eksplan dalam menghasilkan jumlah ES dan skor ES primer dan kurang mempengaruhi jumlah ES dan skor ES sekunder. Pada tahap proliferasi, jumlah ES sekunder relatif tertinggi, dari galur R-036 pada 2,5 ppm 2,4-d, sedangkan varietas Dewata dan R-040 berturut-turut pada 2,5 ppm dan 3,0 ppm 2,4-d. Aplikasi 2,4-d yang dikombinasikan dengan BAP dapat menurunkan jumlah ES, tetapi hanya cenderung menurunkan skor ES sekunder pada ketiga genotipe. Pada skor ES hasil proliferasi tidak diamati adanya pengaruh komposisi ZPT yang diteliti (2,4-d dan BAP) pada varietas Dewata dan galur R-040, sedangkan pada galur R-036 skor ES sekunder maksimum pada 2,5 ppm 2,4-d.
Chitinase Activity in Healthy and Sclerotium rolfsii Infected Peanut ENDANG PUDJIHARTATI; SISWANTO SISWANTO; SATRIYAS ILYAS; SUDARSONO SUDARSONO
HAYATI Journal of Biosciences Vol. 13 No. 2 (2006): June 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (62.34 KB) | DOI: 10.4308/hjb.13.2.73

Abstract

The objectives of this experiment were to analyze the endo- or exo-chitinase activities of healthy and Sclerotium rolfsii infected peanuts. The experiment analyzed 24 different peanut genotypes. Results of the experiment showed chromogenic dimer was the most suitable substrate for analysing chitinase activities. Both endo- and exo-chitinases activities were detected in leaf, stem, and crown tissues. Increased in chitinase activities were detected in S. rolfsii infected peanut tissues than in healthy plant. Regression analysis showed negative slope between disease intensity and chitinase activity in S. rolfsii infected peanut tissue (R2= 0.45). Key words: Disease response, stem rot disease, endo- and exo-chitinase
Oxidative Burst, Peroxidase Activity, and Lignin Content of Sclerotium rolfsii Infected Peanut Tissue ENDANG PUDJIHARTATI; SATRIYAS ILYAS; SUDARSONO SUDARSONO
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.142 KB) | DOI: 10.4308/hjb.13.4.166

Abstract

The objectives of this experiment were to analyse physiological responses, such as oxidative burst reaction, peroxidase activity, and lignin content of healthy and S. rolfsii-infected peanut tissues. Differences in physiological responses among 24 peanut genotypes were determined, the disease severity was calculated and used to group resistance of tested genotypes. The regressions among observed peroxidase activity, lignin content and disease severity were used to determine the possible mechanisms of S. rolfsii resistance in peanut. Peanut seeds were grown in polybag and the growing plants were inoculated at the crown, stem, and leaf tissues. Results of the experiment indicated that infection of S. rolfsii in peanut did not induce oxidative burst. However, infection of the pathogen resulted in increased peroxidase activity and lignin content in the infected tissues. Regression analysis between peroxidase activity and disease severity showed negative slopes, indicating the more resistance the genotype, the more peroxidase activity in the tissue. Regression analysis between lignin content and disease severity was not significant. Key words: hypersensitive response (HR), resistance mechanisms, Sclerotium stem rot, disease response, Arachis hypogaea
Evaluasi Kualitas Estetika Taman Balekambang Solo berdasarkan Pengalaman Berkunjung Erlitha Rahmawati; Endang Pudjihartati
E-JURNAL ARSITEKTUR LANSEKAP Vol.8, No.1, April 2022
Publisher : Prodi Arsitektur Pertamanan, Fakultas Pertanian, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JAL.2022.v08.i01.p13

Abstract

Evaluation of the aesthetic quality of Balekambang Solo Park based on visiting experience. Big Cities such as Solo City have green open spaces such as the Balekambang Park, safe anda beautiful park is the main point to be likes by people. Aesthetic quality is one of the factor that everything becomes beautiful. The aim of this research is to evaluate the aesthethic quality of Balekambang Park Solo based on the experience of traveling. Method this research was conducted by taking landscape photos and performing aesthetic quality assessments by distributing online questionnaires. Then, a division of respondents who had been visited was carried out with responden who had never been. The results of the aesthetics quality evaluation, based on the experience of visiting, makes a difference. The differences can be seen in several areas of Balekambang Park such as the Playground Area. Partinah Bosch Area, Rocky Road Area, Open Space Area anda Artificial Lake Area. The Playground Area there area 2 landscape that have different results, Partinah Bosch Area has 4 landscape, Rocky Road Area has 2 landscape, Open Space area has 3 landscape, and Artificial Lake area has 2 landascape. The difference in these results can be caused that respondents who have not vivisted only judge form the picture, while respondents who visit the area understand the layout or location the area so that they can remember the uniqueness and the condition of the area.
Analisis Persepsi, Preferensi, dan Ekspektasi Perancangan Children Playground Panti Asuhan Salib Putih Salatiga Yeni Setiyawan; Endang Pudjihartati
Jurnal Arsitektur Lansekap Vol.7, No.2, Oktober 2021
Publisher : Prodi Arsitektur Pertamanan, Fakultas Pertanian, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JAL.2021.v07.i02.p11

Abstract

Analysis of perceptions, preferences, and expectations for the design of the children playground at the Salib Putih Orphanage in Salatiga. Playing is a need for children that must be fulfilled. Children can improve gross and fine motoric development, improve reasoning, and understand their environment, shape theirimagination, fantasy power, and creativity by playing. At this time, the Salib Putih Orphanage did not have a special play area for children, the children play in the yard and hall. This study aims to determine the potential and constraints on the site of the Salib Putih Orphanage and to determine the perceptions, preferences, and expectations of stakeholders in the orphanage regarding the design of the children playground which can then be used as input in designing the play area at the Salib Putih Orphanage. The research stages included site surveys, interviews with orphanage caregivers and foundation administrators as well as questionnaires to 40 respondents covering the residents of orphanages, the children and adolescents, with educational levels ranging from elementary school, junior high school to higher education, the orphanage foundation staff and workers, also the practical students at the orphanage. Site survey includes location, boundaries, accessibility, climate. Interviews use the in-depth interview method, which is the process of extracting information in depth, openly, and free of problems and research focus, while the questionnaire for respondents using the Likert measurement scale is presented with several questions that must be filled, given 5 choices that have gradations levels from Strongly Disagree, Disagree, Neutral, Agree to Strongly Agree. Each of these answer choices hasa weighted value of 1-5 points. The results showed that the site in the area of the Salib Putih Orphanage has the potential to build a children’s playground with a variety of facilities and supports in accordance with the analysis of perceptions, preferences, and expectations.
Pengaruh Pemangkasan Cabang dan Mikoriza terhadap Produksi dan Mutu Benih Mentimun (Cucumis Sativus L.) ANWI APRILIANA; ENDANG PUDJIHARTATI; HARMASTINI SUKIMAN
Agrotrop : Journal on Agriculture Science Vol 9 No 1 (2019)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.267 KB) | DOI: 10.24843/AJoAS.2019.v09.i01.p06

Abstract

The Effect of Branch Pruning and Mycorrhiza on Production and Seed Quality of Cucumis sativus L. The purpose of this study was to obtain the best branch pruning, with or without mycorrhiza application, in order to increase the production and quality of cucumber seeds. Pruning the branch at the beginning of cucumber growth is expected to delay fruit formation and support good vegetative growth. This research was carried out in the PT. Primasid Andalan Utama seed production area in Kalibeji Village, Tuntang District, Semarang Regency, Seed Technology Laboratory and Plant Physiology Laboratory, Faculty of Agriculture and Business, Satya Wacana Christian University. This study was conducted by the split-plot randomized complete block design with 2 factors. The main plot consists of 2 levels, namely control/without mycorrhiza (M0) and mycorrhiza application (M1). Pruning the branches as the subplots consist of 4 levels, were pruning to the second, third, fourth and fifth branches of the cucumber plant (P1, P2, P3 and P4). DMRT was used to differentiate the significance of the treatment. The results showed that mycorrhiza application increased the percentage of root infections. The pruning to the fifth branch (P4) is the best; in control without mycorrhiza (M0P4) increased for the number of fruits, the seed weight per plant, and the germination simultaneously; whereas in mycorrhiza application (M1P4) increased the number of fruits and the seed weight per plant. It is also observed that the pruning to the second branch (P1) increased the weight of 1000 grains, the speed of germination and the simultaneous of germination; both with and without mycorrhiza application.
Pengaruh Perlakuan Organomatrixpriming Terhadap Peningkatan Mutu Fisiologis Benih Cabai (Capsicum annuum L.) Fatma Rosita Ratnaningtyas; Endang Pudjihartati
Buletin Anatomi dan Fisiologi Volume 4, Nomor 1, Tahun 2019
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (842.02 KB) | DOI: 10.14710/baf.4.1.2019.45-54

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh organomatrixpriming dalam memperbaiki mutu fisiologis benih cabai. Benih yang digunakan adalah benih cabai varietas Ciko yang telah disimpan selama dua tahun dengan daya berkecambah 74,67%. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan faktor tunggal. Terdapat delapan perlakuan dan lima ulangan. Bahan organomatrixpriming yang diujikan adalah akuades, air kelapa, pupuk organik cair plus, tauge, tauge fermentasi, bonggol pisang, bonggol pisang fermentasi. Organomatrixpriming dilakukan menggunakan media zeolit selama 4 hari, kemudian dilanjutkan dengan pengujian perkecambahan menggunakan metode UKDdp. Hasil penelitian menunjukkan bahwa pemberian perlakuan pupuk organik cair plus dan bonggol pisang fermentasi dapat memperbaiki viabilitas benih cabai serta memenuhi standar daya berkecambah benih menurut Balitsa dan SNI yaitu ≥85%. Tetapi organomatrixpriming yang lain tidak berpengaruh nyata terhadap peningkatan viabilitas dan vigor benih. Perlakuan tauge fermentasi justru menurunkan seluruh variabel mutu fisiologis. Infeksi Aspergillus yang menyebabkan benih busuk dan tumbuh abnormal saat pengujian perkecambahan paling tinggi terdapat pada perlakuan tauge.  Kata kunci : benih cabai, organomatrixpriming, viabilitas, vigor