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All Journal Jurnal Kedokteran Hewan Jurnal Ilmiah Mahasiswa Veteriner Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences)
Erwin Erwin
Faculty Of Veterinary Medice, Universitas Syiah Kuala
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Veterinary

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MENCIT (Mus musculus) GALUR BALB-C YANG DIINDUKSIKAN STREPTOZOTOSIN BERULANG SEBAGAI HEWAN MODEL DIABETES MELITUS Erwin e; Etriwati e; Rusli r
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i1.352

Abstract

Penelitian ini bertujuan mengetahui persentase peningkatan sel beta Langerhans pankreas yang mengalami nekrosis setelah diinduksi streptozotosin berulang.Tiga puluh ekor mencit jantan galur Balb-c, umur 12-14 minggu dengan bobot badan 30-40 g dikelompokkan menjadi 2 kelompok perlakuan.Kelompok I (KI)diberikan pelarut streptozotosin danKelompok II (KII) diberikan streptozotosin dengan dosis 40 mg/kg bb dalam 50 mM natrium sitrat buffer pH 4,5.Semua perlakuan diberikan secara intraperitoneum selama 5 hari berturut-turut. Pemeriksaan kadar glukosa darah dan berat badan dilakukan pada hari ke-0, 7, 14, 21, dan 28 sesudah pemberian perlakuan. Hewan percobaan dari masing-masing kelompok dieutanasiasebanyak 2 ekor pada hari ke-7, 14, 21, dan 28 setelah pemberian perlakuan untuk pewarnaan Gomori. Data pengamatan berat badan adalah42,86±2,3 vs 32,78±7,4 gdan kadar glukosa darah puasa 95,89±2,5 vs 255,77±87,60 mg/dl menunjukkan perbedaan yang signifikan (P0,05) antara kedua kelompok perlakuan, sedangkan persentase sel beta yang mengalami nekrosis antara kedua kelompok perlakuan 4,57±0,47 vs 64,11±4,10juga menunjukkan perbedaan yang signifikan (P0,05). Induksi dosis rendah streptozotosin secara berulang dapat menyebabkan peningkatan persentase nekrosis sel beta Langerhans pankreas.
PROFIL DARAH KUCING LOKAL (Felis catus) SELAMA KESEMBUHAN DISTANT FLAPS akbar muzaky; Erwin Erwin; Herrialfian Herrialfian; M Nur Salim; Tongku Nizwan Siregar
JURNAL ILMIAH MAHASISWA VETERINER Vol 5, No 3 (2021): MEI-JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v5i3.12107

Abstract

ABSTRAKPerubahan nilai profil darah merupakan salah satu indikasi gangguan fisiologis tubuh. Penelitian ini bertujuan mengetahui perubahan nilai profil darah kucing lokal (Felis catus) selama kesembuhan distant flaps. Penelitian ini menggunakan tiga ekor kucing lokal jantan. Semua kucing dibuat luka 2 × 2 cm pada ekstremitas bagian depan, untuk kemudian ditangani dengan teknik distant flap yang bersumber dari area thorak. Pengambilan darah pada hari ke-0 sebelum bedah dan hari ke-3, 6, 9, dan 12 setelah bedah distant flap. Pemeriksaan nilai profil darah meliputi eritrosit, leukosit, hemoglobin, hematokrit, trombosit, dan diferensial leukosit (granulosit, monosit dan limfosit). Hasil pengamatan menunjukkan nilai profil darah kucing mengalami fluktuasi sepanjang hari pengamatan. Peningkatan dan penurunan nilai profil darah merupakan respon tubuh terhadap kesembuhan distant flaps. Distant flap dapat digunakan untuk penutupan luka area ekstremitas pada kucing. Kata kunci : profil darah, kucing, distant flap ABSTRACTChanges in blood profile values are one indication of the physiological disturbances of the body. This study aims to determine the changes in blood profile of local cats (Felis catus) during the healing of distant flaps. This study used three male local cats. All cats were made 2 × 2 cm wound on the front extremities, they then were treated with distant flap technique originating from the thorax area. Blood collection was carried out on day 0 before surgery and days 3, 6, 9, and 12 after distant flap surgery. The blood tests included erythrocytes, leukocytes, hemoglobin, hematocrit, platelets, and differential leukocytes (granulocytes, monocytes and lymphocytes). The observations showed that the value of a cat's blood profile experienced fluctuations throughout the observation day. The increase and decrease in blood profile value is the body's response to the healing of distant flaps. Distant flap can be used for wound closure of the extremity area in cats. Keywords : blood profile, cat, distant flap
Profil Hematologi Kelinci Setelah Implan Plate Material Logam (Profile Hematology Of Rabbit After Metal Plate Implant) Yolanda Sari; erwin erwin; Nuzul Asmilia; Zuhrawaty Zuhrawaty; Amiruddin Amiruddin; Muhammad Hambal
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 1 (2021): NOVEMBER-JANUARI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i1.11797

Abstract

ABSTRAKPenelitian ini bertujuan mengamati jumlah eritrosit, hemoglobin, hematokrit dan trombosit kelinci setelah implan plate material logam. Penelitian ini menggunakan 6 ekor kelinci yang dibagi menjadi 2 kelompok. Kelompok 1 diimplan plate tantalum dan kelompok 2 diimplan plate besi. Pengambilan darah melalui vena auricularis pada hari ke 0, 7, 14, 28, dan 56. Setelah perlakuan jumlah eritrosit, hemoglobin, hematokrit dan trombosit dihitung menggunakan hematology analyzer. Hasil penelitian menunjukkan penurunan jumlah eritrosit, hemoglobin dan hematokrit setelah implan material logam dengan perbedaan tidak signifikan antar waktu pengamatan sedangkan jumlah trombosit menunjukkan perbedaan signifikan antar waktu pengamatan. Implan plate tantalum dan plate besi menyebabkan perubahan jumlah eritrosit, hemoglobin, hematokrit dan trombosit pada beberapa hari pengamatan. Kata kunci : eritrosit, hemoglobin, hematokrit, plate besi, plate tantalumABSTRACT            This study was aimed to determine the number of erythrocytes, hemoglobin, hematocrit and thrombocytes on rabbits after metal plate implant. This study used 6 rabbits that divided into 2 groups. Group 1 was implanted of tantalum plate and group 2 was implanted of iron plate. Taking blood through auricular vein on days 0, 7, 14, 28, dan 56. After treatment the number of erythrocytes, hemoglobin, hematocrit and thrombocytes were calculated with the hematology analyzer. The results showed a decrease in the number of  erythrocytes, hemoglobin and hematocrit after metal plate implant with no significant difference between the time of observation while trombocytes showed a significant difference between the time of observation. Tantalum implant and iron plate causes changes in the number of erythrocyte, hemoglobin, hematocrit and thrombocyte on several days of observation. Keywords: total of erythrocyte, hemoglobin, hematocrit, thrombocyte, iron plate, tantalum plate
UJI DAYA HAMBAT EKSTRAK ETANOL DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP PERTUMBUHAN Staphylococcus aureus (INHIBITORY TEST OF ETHANOL EXTRACT OF NONI LEAF (MORINDA CITRIFOLIA L.) ON STAPHYLOCOCCUS AUREUS GROWTH) Erina Erina; Rinidar Rinidar; T Armansyah; Erwin Erwin; Rusli Rusli; Radhika Elsavira
JURNAL ILMIAH MAHASISWA VETERINER Vol 3, No 3 (2019): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.868 KB) | DOI: 10.21157/jim vet..v3i3.11377

Abstract

ABSTRAKMengkudu (Morinda citrifolia L.) merupakan tanaman khas daerah tropis termasuk Indonesia, yang buah, daun, biji dan akarnya sering digunakan dalam pengobatan tradisional, di antaranya sebagai antibakteri. Penelitian ini dilakukan bertujuan mengetahui kepekaan ekstrak daun mengkudu (Morinda citrifolia L.) terhadap pertumbuhan Staphylococcus aureus. Esktrak daun mengkudu dibagi menjadi 3 konsentrasi yang berbeda yaitu 25%, 50%, 75% dengan penicilin sebagai kontrol positif dan akuades sebagai kontrol negatif. Uji daya hambat dilakukan sesuai dengan metode Kirby-Bauer dengan 3 kali ulangan. Hasil rata-rata zona hambat ekstrak daun mengkudu terhadap bakteri Staphylococcus aureus pada konsentrasi 25% yaitu 6,35 mm, pada konsentrasi 50% yaitu 6,73 mm, dan pada konsentrasi 75% yaitu  6,86 mm. Kontrol positif antibiotik penicilin diperoleh rata-rata 14,28 mm dan kontrol negatif akuades diperoleh rata-rata 0,00 mm. Dapat disimpulkan bahwa ekstrak etanol daun mengkudu memiliki zona hambat sedang terhadap pertumbuhan Staphylococcus aureus sesuai dengan CLSI.Kata kunci: antibakteri, daya hambat, ekstrak daun mengkudu, Staphylococcus  aureus.   ABSTRACT Noni (Morinda citrifolia L.) is one of the most common plants in tropical areas, including Indonesia, which fruit, leaves, seed and root, are used in traditional treatment, for an example  antibacterial. The aims of this research is to know the sensitivity of noni leaf extract (Morinda citrifolia L.) against Staphylococcus aureus growth. Noni leaf extract was divided into 3 different concentrations 25%, 50%, 75%. Penicilin used as control positive and Aquadest used as control negative. The inhibition effect test was performed in accordance to Kirby-Bauer method with 3 replications. The average of inhibition zone of noni leaf extract on Staphylococcus aureus at 25% concentration was 6,35 mm, at concentration of 50% was 6,73 mm, and at concentration 75% was 6.86 mm. The averaged positive control of penicilin antibiotics was 14,28 mm and negative control of aquadest was 0,00 mm. It can be concluded that the ethanol extract of noni leaves had fair activity to inhibit the growth of Staphylococcus aureus according to CLSI.Keyword : antibacterial, inhibition effect, noni leaf extract, Staphylococcus aureus.
NILAI SERUM GLUTAMIC OXALOACETIC TRANSAMINASE (SGOT) DAN SERUM GLUTAMIC PYRUVIC TRANSAMINASE (SGPT) KELINCI SETELAH IMPLAN PLATE BERBAHAN LOGAM (THE VALUE OF SERUM GLUTAMIC OXALOACETIC TRANSAMINASE (SGOT) AND GLUTAMIC PYRUVIC TRANSAMINASE (SGPT) OF RABBITS AFTER METAL PLATES IMPLANT) Anta Rezky Sitepu; Erwin Erwin; Muhammad Hasan; Sugito Sugito; Al Azhar; Razali Razali
JURNAL ILMIAH MAHASISWA VETERINER Vol 4, No 2 (2020): FEBRUARI-APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v4i2.11413

Abstract

ABSTRAK                Penelitian ini bertujuan untuk mengetahui perubahan nilai Serum Glutamic Oxaloacetic Transaminase (SGOT) dan Serum Glutamic Pyruvic Transaminase (SGPT) kelinci setelah implan plate berbahan logam. Penelitian ini menggunakan 6 ekor kelinci yang dibagi menjadi 2 kelompok. Kelompok 1 diimplan plate berbahan tantalum dan kelompok 2 diimplan plate berbahan besi. Pengambilan darah melalui vena auricularis dilakukan pada hari ke 0, 7, 14, 28, dan 56. Hasil penelitian menunjukkan nilai SGOT dan SGPT meningkat pada beberapa hari pertama pengamatan dan menurun kembali pada beberapa hari terakhir pengamatan yang masih dalam batas normal. Penggunaan implan plate tantalum dan plate besi alternatif menyebabkan perubahan nilai SGOT dan SGPT pada beberapa hari pengamatan.Kata Kunci : SGOT, SGPT, Plate, Tantalum, Besi ABSTRACT            This study aims to determine changes in Serum Glutamic Oxaloacetic Transaminase (SGOT) and Glutamic Pyruvic Transaminase (SGPT) values of rabbit after a metal implant plate. This study used 6 rabbits divided into 2 groups. Group one implanted a plate made from tantalum and group two implanted with alternative iron plates. Blood collection through the auricular vein was carried out on days 0, 7, 14, 28, and 56. The results showed that the value of SGOT and SGPT increased in several days of observation and increased again on the final day which was still within normal limits. The use of tantalum implants and alternative iron plates causes changes in the value of SGOT and SGPT on several days of observation.Keyword : SGOT, SGPT, Plate, Tantalum, Iron
Kajian Molekuler Karakteristik Gen Calpastatin (CAST) pada Kambing Boerka (Capra Hircus) sri yuliati; T. Zahrial Helmi; Rinidar Rinidar; Ummu Balqis; erwin erwin; Rosmaidar Rosmaidar
JURNAL ILMIAH MAHASISWA VETERINER Vol 3, No 4 (2019): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v3i4.7831

Abstract

ABSTRAKGen yang yang dapat mempengaruhi keempukan daging salah satunya adalah gen calpastatin (CAST). Tujuan penelitian ini adalah untuk mengetahui karakteristik gen CAST pada kambing boerka secara molekuler. Sampel penelitian yang digunakan adalah darah yang diambil dari 5 ekor kambing boerka yang dipelihara di UPT Hewan Coba Universitas Syiah Kuala. Metode yang digunakan yaitu dengan polymerase chain reaction (PCR), elektroforesis dan sekuensing DNA. Gen CAST pada kambing boerka berhasil diamplifikasi dengan suhu 59°C sebesar 1552 bp. Sebanyak 5 sampel yang telah di PCR dilakukan sekuensing. Hasil sekuensing dianalasis menggunakan software program Basic Local Alignment Search Tool (BLAST) dan Clustal W dari program Mega 6.01. Hasil analisis menunjukkan bahwa gen CAST pada kambing boerka memiliki kemiripan dengan gen CAST pada Ovis  aries dan Bos taurus. Karakteristik gen CAST pada kambing boerka diantaranya memiliki panjang pasangan basa yang dapat teramplifikasi sebesar 1552 bp menggunakan suhu annealing 59°C dan memiliki kemiripan sekuen dengan gen CAST pada Ovis aries dan Bos taurus sesuai data yang ada di Genbank serta adanya variasi jarak genetik antar sampel kambing boerka.Kata kunci : Calpastatin, PCR, sekuensing , kambing boerka. ABSTRACT                Genes that can affect the tenderness of one of them is the calpastatin (CAST) gene. The aim of this research was to find out the characteristic of CAST gene in boerka goats. The sample of research used blood collected from 5 boerka goats which kept in UPT Hewan Coba Syiah Kuala University. The method used with polymerase chain reaction (PCR), elektoforesis and DNA sequencing. CAST gene on boerka goats was successfully amplified with a temperature of 59 ° C for 1552 bp. A total 5 samples have been in PCR in sequencing. The results of sequencing in analytics using software Local Basic Alignment Search Tool (BLAST) and Clustal W program from Mega 6.01 program. The results showed that the CAST gene in boerka goats had similarities with the CAST gene in Ovis aries and Bos taurus . Characteristics CAST gene in boerka goats such as having an alkaline pair length that can be amplified by 1552 bp using annealing temperature of 59 ° C and sequence similarity with CAST gene on Ovis aries and Bos taurus according to existing data in Genbank and the variation of genetic distance between samples of boerka goats.Keywords : Calpastatin, PCR, sequensing, boerka goat    
Profil Darah Pada Kucing Selama Proses Kesembuhan Luka Melalui Teknik Skin Flaps Rotasi Yang Dirawat Dengan Dry Dressing Dan Moist Dressing: Blood Profile of Cats during the Wound Healing Process using the Rotation Flap with both Dry and Moist Dressing Erwin Erwin; Rusli Rusli; Fidella Diva Jones
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 10 No. 1 (2020): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v10i1.83

Abstract

The skin flap is one of the techniques employed to reconstruct defects and close wounds. This study aims to observe the blood profile in cats during the healing process of rotation skin flaps that were treated with a dry and moist dressing. This study used six male cats within the age of 1-2 years old and weighed among 2-4 kg, divided into two treatment groups. All of the specimens were treated with the following procedure; the fur on the abdominal area was shaved and disinfectant was applied to the area between the os sacrum and os femur, lastly, 2 cm2 triangle wound was deliberately made on the area. The treatment of the wound was done by the rotation skin flaps method. Group 1 was treated with sterile bandage and group 2 was treated with sofratulle®. Blood samples were taken from the cephalica antebrachii vein on the 1st, 3rd, 6th, and 12th days. The erythrocytes, hemoglobin, hematocrit, platelets, leukocytes, neutrophils, basophils, eosinophils, monocytes, and lymphocytes were counted by hematology analyzer. The results showed that there is a significant difference (P<0.05) in the increase of neutrophil levels between the group treated with sterile kassa (P-1) and the group treated with sofratulle® (P-2). The number of the cats’ red blood cells rose on the 3rd day and rose again on the 12th day after the treatment with a significant difference (P<0.05) during the observation. In conclusion, the rotation flaps treatment method with dry dressing and moist dressing in the wound healing process affected the cats’ blood profile and the moist dressing was preferred to the dry dressing.
Gambaran Histopatologi Hati Tikus Putih (Rattus Norvegicus) Setelah Pemasangan Implan Wire Material Logam Seftia Yolanda; Etriwati Etriwati; Erwin Erwin; Dian Masyitha; Roslizawaty Roslizawaty; Arman Sayuti
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 4 (2022): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i4.16667

Abstract

ABSTRAK Penelitian ini bertujuan untuk melihat gambaran histopatologi hati tikus putih (Rattus norvegicus) setelah diimplan wire komersial dan wire alternatif  pada tulang femur. Penelitian ini menggunakan 10 ekor tikus putih yang dibagi menjadi 2 kelompok perlakuan. Kelompok perlakuan 1 diimplan dengan wire komersial (SS316L) dan kelompok perlakuan 2 diimplan dengan wire alternatif (benang pancing). Hari ke-86 setelah perlakuan, semua tikus dieutanasia menggunakan ketamine. Jaringan hati diambil untuk pembuatan sediaan histopatologi dalam blok parafin. Gambaran histopatologi hati tikus yang diimplan dengan kelompok wire komersial dan wire alternatif  menunjukkan perubahan yaitu adanya hiperemi, hemoragi, nekrosis dan sel-sel inflamasi dengan perbedaan yang tidak signifikan (P0,05). Gambaran histopatologi hati tikus yang diimplan dengan wire alternatif tidak berbeda dengan wire komersial.ABSTRACT This study aims to look at the histopathological picture of the liver of white rat (Rattus norvegicus) after implanting commercial and alternative wires in the femur bone. This study used 10 white rats which were divided into 2 groups. Treatment group 1 is implanted with commercial wire (SS316L) and consultation group 2 is implanted with alternative wire (fishing line). The 86th day after treatment, all rats were dehydrated using ketamine. Liver tissue is taken to make histopathological preparations in paraffin blocks. Histopathological features of rat liver implanted with commercial and alternative wire groups showed hyperemia, hemorrhage, necrosis and inflammatory cells with insignificant differences (P 0.05). Histopathological picture of rat liver implanted with wire is no different from commercial wire.
Co-Authors akbar muzaky Al Azhar Amiruddin Amiruddin Anta Rezky Sitepu Arman Sayuti Dian Masyitha Erina Erina Etriwati E Fidella Diva Jones Herrialfian Herrialfian M Nur Salim Muhammad Hambal Muhammad Hasan Nuzul Asmilia Radhika Elsavira Razali Razali Rinidar Rinidar Rinidar Rinidar Roslizawaty Roslizawaty Rosmaidar Rosmaidar Rusli r Rusli Rusli Rusli Rusli Seftia Yolanda sri yuliati Sugito Sugito T Armansyah T. Zahrial Helmi Tongku Nizwan Siregar Ummu Balqis Yolanda Sari Zuhrawaty Zuhrawaty