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All Journal The International Journal of Tropical Veterinary and Biomedical Research
Elly L. Rustiati
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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DNA Isolation on Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park: A First Step towards Its ID Card Elly L. Rustiati; Priyambodo Priyambodo; Siti Asiyah; Dedi Candra; Diah E. Anggraini; Elizabeth D. Krismuniarti; Eko Agus Srihanto; Liza Angeliya; Nuning Nurcahyani; Enny Saswiyanti
The International Journal of Tropical Veterinary and Biomedical Research Vol 3, No 1 (2018): Vol. 3 (1) May 2018
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (189.698 KB) | DOI: 10.21157/ijtvbr.v3i1.11368

Abstract

Elephant Training Center (ETC) Way Kambas National Park (WKNP) was built to support human-elephantmitigation conflict. The small population of captive sumatran elephant in ETC WKNP need a comprehensivestrategy in order to maintain the genetic variation of each individual and avoid inbreeding drive. Currently, geneticstudies have opened new field studies in ecology, included conservation ecology. Patterns in variation of populationhas been investigated by molecular method supporting species conservation effort. The captive sumatran elephant’sID Card is a necessary in database building, which included morphology, health status, and genetic profile. Geneticprofile in each ID Card was filled by cytogenetic and molecular profile for RADP result, that initiated with DNAisolation. The DNA sources collected by blood sampling protocol described by Asiyah et al. (2016) from captivesumatran elephant in ETC, WKNP, and be carried to laboratory in cold condition. The DNA sources stored at 4oCand isolated following commercial protocol. The result of DNA isolation stored at -20oC until amplificationanalysis. DNA isolation was successfully done, for further individual genetic ID building.
Comparative Cytogenetic Study on Male and Female Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park Priyambodo Priyambodo; Elly L. Rustiati
The International Journal of Tropical Veterinary and Biomedical Research Vol 3, No 1 (2018): Vol. 3 (1) May 2018
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.059 KB) | DOI: 10.21157/ijtvbr.v3i1.11333

Abstract

Sumatran elephant (Elephas maximus sumatranus) was one of five big mammals in Way Kambas National Park (WKNP). Cytogenetic study was a part of conservation effort for sumatran elephant, especially in conservation genetic field. Captive sumatran elephant’s blood in Elephant Training Center (ETC) WKNP was collected to karyotype analysis. Captive sumatran elephant’s chromosome was analyzed by squashing technique with pretreating cells in a hypotonic solution. The study of karyotypes were made by giemsa staining applied to captive elephant’s blood cell. The diploid number of captive sumatran elephant was 56 both in male and female. The karyotype analysis performed different fundamental number in male and female, both of total fundamental number (FN) and autosomal fundamental number (FNa). Chromosome of female sumatran elephant showed the FN = 8, and FNa = 6, but in male sumatran elephant FN = 7, and FNa = . The karyotypes of X chromosome was large submetacentric and Y chromosome was small acrocentric.. The karyotype presented here will compilled with DNA fingerprinting analysis of all captive sumatran elephant in ETC WKNP as references in future breeding policy.
Co-Authors A.Basyir Firdaus AGUS SETIAWAN Anak Agung Istri Sri Wiadnyani DEDI CANDRA Diah E. Anggraini Eko Agus Srihanto Elizabeth D. Krismuniarti Enny Saswiyanti Liza Angeliya Nuning Nurcahyani Priyambodo Priyambodo Siti Asiyah