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Journal : Journal of Microbial Systematics and Biotechnology

PCR-based Specific Detection of Bacillus in Liquid Organic Fertilizer Hidayat, Iman; Laili, Nur; Agustiyani, Dwi; Antonius, Sarjiya
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.783 KB) | DOI: 10.37604/jmsb.v1i1.21


Rapid molecular PCR-based detection method for Bacillus species used in the production of Beyonic® liquid organic fertilizer was carried out based on nucleotide sequence data from the 16S rRNA gene. The method involved sequencing the 16S rRNA gene of several Bacillus species and identifying around 16-22 specific nucleotide bases from 5' and 3' ends in the Bacillus 16S rRNA gene sequences. One specific primer pair for Bacillus detection was determined as follow: 5' - CAT AAG ACT GGG ATA ACT CCG GG - 3' (forward) from positions of 85-107 bp, and 5’ - CCA GGC GGA GTG CTT AAT GC - 3’ (reverse) from positions of 836-854 bp. PCR assay and gel electrophoresis analysis showed that the primer pair was specific to the genus Bacillus.