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All Journal J@TI (TEKNIK INDUSTRI) Berkala Ilmiah Pendidikan Biologi (BioEdu) JTSL (Jurnal Tanah dan Sumberdaya Lahan) Journal of S&T Policy and R&D Management BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Kesehatan Bakti Tunas Husada Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat Journal on Education Islamic Guidance and Counseling Journal Journal of Microbial Systematics and Biotechnology Jurnal Ilmu Farmasi dan Farmasi Klinik Attractive : Innovative Education Journal
Nur Laili
Pusat Penelitian Perkembangan Iptek Lembaga Ilmu Pengetahuan Indonesia (Pappiptek-LIPI)
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Public Health Social Sciences Other

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Journal : Journal of Microbial Systematics and Biotechnology

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PCR-based Specific Detection of Bacillus in Liquid Organic Fertilizer Hidayat, Iman; Laili, Nur; Agustiyani, Dwi; Antonius, Sarjiya
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.783 KB) | DOI: 10.37604/jmsb.v1i1.21

Abstract

Rapid molecular PCR-based detection method for Bacillus species used in the production of Beyonic® liquid organic fertilizer was carried out based on nucleotide sequence data from the 16S rRNA gene. The method involved sequencing the 16S rRNA gene of several Bacillus species and identifying around 16-22 specific nucleotide bases from 5' and 3' ends in the Bacillus 16S rRNA gene sequences. One specific primer pair for Bacillus detection was determined as follow: 5' - CAT AAG ACT GGG ATA ACT CCG GG - 3' (forward) from positions of 85-107 bp, and 5’ - CCA GGC GGA GTG CTT AAT GC - 3’ (reverse) from positions of 836-854 bp. PCR assay and gel electrophoresis analysis showed that the primer pair was specific to the genus Bacillus.
Co-Authors Abimanto, Dhanan Andi Salamah Aprianti, Rifdah Arista, Ratny Witanti Aziz Q, Ikhwan Cholida, Diana Dwi Agustiyani Eko HANDAYANTO Hartati Imamuddin Hendri Tanjung Iis Lestari, Iis Iman Hidayat K.M., Dewi Andini Kirana Rukmayuninda Ririh Maman Rahmansyah Mimiek Murrukmihadi Ria Kurniawati Sarjiya Antonius Setiani, Annisa Isna Shirly Rizki Kusumaningrum, Shirly Rizki Silmi Tsurayya, Silmi Sri Hartati Wahyu Kusumaningtyas Wicaksono, Adityo Yuliani Yuliani