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Lack of Atherosclerotic Lesion Progression on Severe Hyperlipidemic Rabbits DIMAS ANDRIANTO; SULISTIYANI SULISTIYANI; LATIFAH KOSIM DARUSMAN; HASIM DANURI
HAYATI Journal of Biosciences Vol. 16 No. 2 (2009): June 2009
Publisher : Bogor Agricultural University, Indonesia

In human, coronary heart disease causes by severe pathological atherosclerosis. In this study, we established animal model to study atherosclerosis caused by hyperlipidemia. This study therefore was undertaken to define the effect of increasing atherosclerosis risk factor, include body weight as well as age, cholesterol concentration and dietary fat in rabbit chow, and time of treatment. Male New Zealand White rabbits were divided into 4 groups; Group I and III were consisted of 2 months rabbit were fed with standard rabbit chow. To introduce atherosclerosis, the chow for Group II was contained 0.25% cholesterol and 5% palm oil; whereas the chow for group IV was contained 0.5% cholesterol and 5% coconut oil to induce higher atherosclerotic lesion. Results showed that group II and IV developed hyperlipidemia. However, aortic cholesterol concentration in those groups did not different significantly (P > 0.05). We suggest that low carbohydrate composition in diet, 50% lower compared to the previous researches, was able to increase high-density lipoprotein (HDL) concentration. This study demonstrated the complex interactions between low carbohydrate diet and cholesterol metabolism and the dramatic effects of reducing atherosclerosis risk factor; however, even though hyperlipidemic condition was achieved, total plasma cholesterol HDL ratio was maintained low. Key words: atherosclerosis, hyperlipidemia, rabbit, low carbohydrate diet

Physiological Response of Crocidolomia pavonana to the Calophyllum soulattri Active Fraction EDY SYAHPUTRA; DJOKO PRIJONO; DADANG DADANG; SYAFRIDA MANUWOTO; LATIFAH KOSIM DARUSMAN
HAYATI Journal of Biosciences Vol. 13 No. 1 (2006): March 2006
Publisher : Bogor Agricultural University, Indonesia

The objective of this study was to evaluate the physiological response of the cabbage head caterpillar Crocidolomia pavonana treated with an active fraction of Calophyllum soulattri bark extract. Extraction of the test plant materials were performed with maceration method using methanol, continued by counter-current distribution separation in ethylacetate and water. Methanol fractionation of C. soulattri was performed by vaccuum liquid chromatography and the bioassays were conducted by a leaf-feeding method. The results showed that the dichloromethane fraction of C. soulattri had strong insecticidal activity against C. pavonana larvae, with LC50 of 0.05%. Sublethal treatments with the active fraction at LC15, LC50, and LC85 reduced the relative growth rate of the fourth instars by 48.9-94.1%. The treatments with the fraction at LC15 and LC50 to the fourth instars reduced the activity of invertase and protease enzyme by 20.7-24.1 and 14.4-25.14%, respectively, but increased the activity of trehalase by 26.7-120% as compared with controls. Key words: Physiological response, Crocidolomia pavonana, Calophyllum soulattri, botanical insecticide

Changes of Gibberellin and Total Sugar Content in Flower Developmental Stages of Mangosteen I NYOMAN RAI; ROEDHY POERWANTO; LATIFAH KOSIM DARUSMAN; BAMBANG SAPTA PURWOKO
HAYATI Journal of Biosciences Vol. 13 No. 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

The objectives of this experiment were to study the changes of gibberellic acid and total sugar content in flower developmental stages of mangosteen. The result showed that flower development of mangosteen consisted of four stages: induction, differentiation, maturition of flower organs, and anthesis. Floral induction was microscopically characterized by the swelling of the basal structure of the new shoot. It was found that induction stage of mangosteen flowering was characterized by sharp decrease of gibberellic acid (GA3, GA5, GA7) and increase of total sugar content of leaf. On the other hand, it was found that leaf of the non-flowering shoot apices had high gibbrellic acid and low total sugar. Key words: mangosteen, flowering, induction, gibberellic acid

The research was carried out to isolate and characterize of bioactive proteins from plant parts of Benincasa hispida (Thunb.) Cogn and to analyse of the toxicity and cytotoxicity of the proteins. The proteins were extracted with phosphate buffer saline, then they were precipitated using 80% saturated ammonium sulphate, continued with the dialysis using pH 7 phosphate buffer. The dialysate was fractionated through gel filtration chromatography and characterized using SDS-PAGE. The toxicity of th CHURIYAH CHURIYAH; LATIFAH KOSIM DARUSMAN
HAYATI Journal of Biosciences Vol. 16 No. 4 (2009): December 2009
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.16.4.161

The research was carried out to isolate and characterize of bioactive proteins from plant parts of Benincasa hispida (Thunb.) Cogn and to analyse of the toxicity and cytotoxicity of the proteins. The proteins were extracted with phosphate buffer saline, then they were precipitated using 80% saturated ammonium sulphate, continued with the dialysis using pH 7 phosphate buffer. The dialysate was fractionated through gel filtration chromatography and characterized using SDS-PAGE. The toxicity of the proteins was analyzed through brine shrimp lethality test (BSLT), followed with cytotoxicity test using HeLa and K-562 cancer cell lines. Three bioactive protein fractions were isolated from the fruits, the seeds and roots. The lowest yield of proteins was 0.021% from the fruit, then 0.051% from the seed, while the highest was 0.54% from the root. All proteins were toxic on BSLT with LC50 within the range of 24-39 µg. Characterization of proteins using SDS-PAGE indicated the molecular mass of those proteins were approximately 17-29 kDa. The cytotoxicity test of the root protein showed that the protein could inhibit proliferation of HeLa cell up to 28.50% and K-562 cell up to 36.60% compared to that of non treated cell. Key words: Benincasa hispida (Thunb.) Cogn, bioactive protein, cytotoxicity

Characterization of an Endophytic Bacterium G062 Isolate with Beneficial Traits ALINA AKHDIYA; ARIS TRI WAHYUDI; ABDUL MUNIF; LATIFAH KOSIM DARUSMAN
HAYATI Journal of Biosciences Vol. 21 No. 4 (2014): December 2014
Publisher : Bogor Agricultural University, Indonesia

An endophytic bacterium isolate G062 was characterized base on its molecular genetic potents, morphology, physiology, and biochemistry reactions. Analysis of 16S rDNA sequences of G062 showed the highest similarity to Paracoccus halophilus (98%). Detection of the phlD and prnC genes occurrence indicated that the bacterium had this antibiotic-like genes of Diacethylphloroglucinol (DAPG) and pyrrolnitrin. The cells are rod shaped (0.59-0.89 x 1.85-3.3 µm), aerobic, Gram negative, non motile, non spore forming, positive catalase, positive oxydase, could reduce NO3 to N2, nitrogen fixing, producing siderophore and plant growth hormones-like compounds (IAA, Gibberellin, and zeatin), and solubilizing phosphate. The G062 isolate could grow on media containing 2.5% NaCl. Range of the temperature and pH growth were 15-40 and 5.0-9.5 oC, respectively. The bacterium did not cause red blood cells lysis. There was no hypersensitive response when it was injected into tobacco leaves, and it was not pathogenic against potato plantlets. Moreover, the bacterium promoted the growth of the potato plant and had high colonization ability. These results suggested that the bacterium had beneficial and good traits as biological agent candidate to promote potato plant growth.

Potensi Daerah Sidik Jari Spektrum Inframerah Sebagai Penanda Bioaktovitas Ekstrak Tanaman Obat Latifah Kosim Darusman; Rudi Heryanto; Mohamad Rafi; Wulan Tri Wahyuni
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 3 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.806 KB)

Efficacy and quality of medicinal plant extracts depend on chemical composition therein. Therefore, to ensure its efficacy orquality, the chemical composition of extracts have to be analyzed based on its chemical marker or its chemical pattern. This study tried to develop a method for extracts quality assay based on infrared spectrum in fingerprint region and extracts inhibition activity to xanthine oxidase. Five extracts from kunyit (Curcuma domestica), temulawak ( C xanthorriza), jahe (Zingiber officina/e), temukunci (Boesenbergia pandurata), and cabe jawa (Piper retrofractum) were tested to inhibit xanthine oxidase activity. The bioactive data of extracts and its infrared spectrum was analyzed using PCA and PLS-DA (partial least squarediscriminant analysis). Almost all extracts that were tested showed inhibition activity to xanthine oxidase activity, except the extract of jahe. The result showed that PCA can group all of extract into each of their region. Meanwhile, PLS-DA has shown to be a good prediction model for classifying extracts based on fingerprint region of its mR Spectrum and its biological activity. Keywords: fingerprint, mR spectra, compound marker, xanthine oxidase

Pengembangan Pupuk Cair Nitrogen Berukuran Nanometer untuk Meningkatkan Efisiensi Pemupukan Deden Saprudin; Munif Gulamahdi; Wiwik Hartatik; Latifah Kosim Darusman; Ilfa Nuraisyah
Jurnal Ilmu Pertanian Indonesia Vol. 17 No. 2 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.569 KB)

Conventional utilization of urea just has absorbed 30-60%. Remaining, 40-70% lost to environment, give not economic benefit and serious problem in environment. The use of less efficient urea has received attention lately. One way to overcome the problem of inefficiency of urea fertilizer use is a nano-sized fertilizer. Synthesized nanofertilizer in this study were nanomagnetit (Fe3O4). Nanomagnetit synthesized from FeCl3 as a source of iron, sodium citrate as the reductant, and urea as a source base. Nanomagnetite synthesized using a hydrothermal method. Based on the results of X-ray diffraction, the nanomagnetit has average crystal size 46.66 nm and crystal structure is Face Center Cubic. Energy-dispersive X-ray spectroscopy shows constituent elements in nanomagnetite are iron (29.45%), oxygen (53.07%), carbon (14.86%), and sodium (2.62%). This shows the iron and oxygen is the main constituent elements nanomagnetite. In the process of magnetite formation absorbed urea by 30% with N content (0.14%) and most are in the liquid synthesis (0.82%) so that it can be used as fertilizer on crops. Based on this research, variations of pH (6-8) liquid fertilizer provided no significant effect. The addition of 0.15 g nanomagnetite have better growth among other treatments.

Senyawa Penciri Ekstrak Daun Jati Belanda (Guazuma ulmifolia Lamk) sebagai Anti-Kolesterol Irmanida Batubara; . Husnawati; Latifah Kosim Darusman; Tohru Mitsunaga
Jurnal Ilmu Pertanian Indonesia Vol. 22 No. 2 (2017): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Jati belanda (Guazuma ulmifolia Lamk) leaves is traditionally used as slimming and cholesterol reducing agent. This research aims to determine the active component on jati belanda leaves as marker compound for cholesterol reducing agent. The quality of leaves was checked prior to use for next step of analysis. Jati belanda leaves with good quality was extracted by ethanol 30% and the extract was separated by chromatography technique such as open column chromatography and preparative high performance liquid chromatography. The results showed that the quality of samples is meet to the requirement of Indonesian Food and Drug Agency (Badan Pengawas Obat dan Makanan (BPOM)) based on moisture content, ash content, and heavy metal content. Based on the separation results showed that the cholesterol reducing compound from jati belanda is quercetin

Induksi Ekstrak Pegagan Secara in vitro terhadap Proliferasi dan Diferensiasi Sel-Sel Otak Besar Anak Tikus (IN VITRO INDUCTION OF CENTELLA ASIATICA (PEGAGAN) EXTRACT ON THE PROLIFERATION AND DIFFERENTIATION OF NEWBORN RAT CORTEX CEREBRI CELLS) Ita Djuwita; Min Rahminiwati; Latifah Kosim Darusman; Siti Sa’diah
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.61 KB)

The aim of the study was to analyze the potency of Centella asiatica extract to induce proliferation andneurogenesis process of newborn rats cortex cerebri. Research has been conducted on in vitro culture ofthree days old rat (Sprague Dawley) cerebrum cells in DMEM (Dulbecco’s Modiûed Eagle’s Medium)containing 10% NEAA (Non Essential Amino Acid), 1 mM NaHCO3, 10% NBCS (Newborn Calf Serum)and 50 µg/mL gentamycin (mDMEM), with and without Centella asiatica (CA) leaf extracts. The experimentwas set in five groups of treatment consisted of positive control (mDMEM+30 µg/mL asiaticoside (AC)),negative control (mDMEM), and mDMEM with three concentration of CA extract i.e. 100 ppm, 200 ppmand 400 ppm. Culture was done in 5% CO2 incubator at 37oC for six days. The parameters observed werecells proliferation based on Population Doubling Time (PDT), neuron and glia composition, and the lengthof axon and dendrite. Cells concentration were counted using Newbauer hemocytometer. Neuron and gliacells were determined based on morphology after Hematoxylin-Eosin staining, and the length of axon anddendrite were measured using eyepiece micrometer. Data were analyzed using ANOVA and Duncan test.The results showed that Centella asiatica extract at concentration 100 ppm could induce neurogenesisand increased the axon length growth. However, at concentration 200 and 400 ppm, CA extract inhibitedthe neuronal cells proliferation and the axonal growth (P<0,05). In conclusion, induction of Centella asiaticaextracts at concentration of 100 ppm on the cortex cell cerebrum cells culture increase the axon lengthgrowth and tends to induce neurogenesis; however at higher concentration CA extract was neurotoxic.

Protein Patterns in Arbuscular Mycorrhizal Roots and Non-Mycorrhizal Roots of Oil Palm Seedling HAPPY WIDIASTUTI; NAMPIAH SUKARNO; LATIFAH KOSIM DARUSMAN
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

A comparison of the protein patterns in root extracts from none mycorrhizal and mycorrhizal oil palm roots has been made. The polypeptides were analyzed every three weeks up to 11 weeks. A factorial design of fungi species (no mycorrhizal, Acaulospora tuberculata, Gigaspora margarita) and with or without fertilizer was assessed. The result showed that specific polypeptides were detected in primary and secondary roots. In unfertilized oil palm root, a 60 kDa polypeptide was detected while it was abcent in fertilized root. Inoculation of A. tuberculata with the addition of fertilizer application yielded a specific 26.7 kDa polypeptide in primary root on the 11th week after inoculation. A specific 64.2 kDa polypeptide of G. margarita was detected in unfertilized secondary root also on the 11th week.

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