BIBIANA W LAY
Department Of Biology, Faculty Of Biotechnology, Atma Jaya Catholic University, Jalan Jenderal Sudirman No. 51, Jakarta 12930, Indonesia

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PEMANFAATAN KONSORSIUM BAKTERI LOKAL UNTUK BIOREMEDIASI LIMBAH TEKSTIL MENGGUNAKAN SISTEM KOMBINASI ANAEROBIK-AEROBIK Sastrawidana, I Dewa K; Lay, Bibiana W; Fauzi, Anas Miftah; Santosa, Dwi Andreas
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1086.061 KB) | DOI: 10.14203/beritabiologi.v9i2.2020

Abstract

The objective of this research is to study the potential use microorganisms which are identified as Aeromonas sp., Pseudomonas $p., Flavobaclerium sp., Plesiomonas sp. and Vibrio sp. Five bacteria strains from sludge of Badung river were identified as Vibrio sp. and Plesiomonas sp. Two anaerobic-aerobic reactors were operated to treat textile waste water. Each reactor contained vulcanic stone to increase spesific surface of media for attachment of bacteria.Bacteria consortia used for anaerobic process consist of Aeromonas sp. (ML6), Aeromonas sp. (ML14), Aeromonas sp. 9ML24), Pseudomonas sp. (ML8) and Flavobacterium sp. (ML20). Whereas, bacteria consortia for aerobic process consist of Plesiomonas sp. (SB1), Plesiomonas sp. (SB2), Vibrio sp.(SB1), Vibrio sp. (SB2) and Vibrio sp. (SB3). The system was operated for 3 day in each reactor. The result showed, biodegradation of textile waste water in combined anaerobic-aerobic system by attached growth process is potential for treatment of textile waste water.This technology is effective to decrease COD value up to 98.38%, BODS 93.90%, TDS 80.87%, TSS 87.50% and decolorization of textile dyes up to 95.57%.
PENGOLAHAN LINDI SEBAGAI PUPUK CAIR UNTUK MENDUKUNG PENGEMBANGAN TPA SAMPAH LESTARI Nurhasanah, Nurhasanah; Darusman, Latifah K; Sutjahjo, Surjono Hadi; Lay, Bibiana Widiati
Jurnal Matematika Sains dan Teknologi Vol 11 No 1 (2010)
Publisher : LPPM Universitas Terbuka

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Abstract

A laboratory scale research to obtain environmentally safe effluent and liquid fertilizer from leachate from Final Waste Disposal Site in Galuga owned by Regional Government of Bogor City was conducted from July 2006 through to April 2007. The experiment was initiated by aerating the leachate in 4 difference aeration rates (0, 10, 30 and 70 liters/minute) followed by processing the sediment to product the liquid fertilizer. The experiment of production liquid fertilizer was carried out by adding lime with different dosage into sediment generated from processing by aerating at 70 liters/minute followed by centrifugation process or secher. Further, the liquid fertilizer generated from such experiment was applied to chilis planting (Capsicum annum). The research found that the processing conducted by aerating at the rate 70 liters/minute was the most effective in reducing pollutant from leachate. The addition of 1000 ppm CaO or Ca(OH)2 limes in sediment from aeration is the most effective in depositing the dissolved material compared to the addition of limes in other dosage. Liquid fertilizer generated through the addition of 1000 ppm CaO have the content of N = 375,83 ppm, P = 121,44 ppm, K = 948,11 ppm, Ca = 827,20 ppm, Mg = 959,50 ppm, S = 48,53 ppm, Cu = 8,23 ppm, Zn = 30,02 ppm, Mn = 230,57 ppm, Fe = 320,95 ppm, Pb = 10,34 ppm, Cd = 7,46 ppm and Cr = 2,05 ppm. The use of liquid fertilizer generated by adding 1000 ppm CaO was the most effective in enhancing vegetation growth and production of chili. The non essential elements (Pb, Cd and Cr) in fruits from vegetation given liquid fertilizer produced from such treatment did not exceed tolerable threshold.
Carotenoid Production by Rhodosporidium paludigenum Using Cassava Starch Hydrolyzed by Bacillus subtilis as Substrate Warjoto, Renna Eliana; Felianti, Felianti; Lay, Bibiana Widiyati
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.36

Abstract

Carotenoids are natural pigments with colors ranging from yellow to red that are beneficial for food, cosmetics, and animal feed industries. These pigments can be found in fruits, vegetables, algae, and microorganisms. Among all microorganisms that have been known to produce carotenoids, Rhodosporidium paludigenum is still poorly investigated. Therefore, this study aimed to determine the potential of carotenoid production by R. paludigenum using cassava starch hydrolyzed by Bacillus subtilis as a substrate. The cassava starch for hydrolysis was divided into four concentrations, i.e., 2%, 4%, 6%, and 8% w/v. During the hydrolysis period, the amylase enzyme activity produced by B. subtilis was evaluated. The reducing sugar concentration was then examined to determine the optimum medium for carotenoid production. The highest amylase enzyme activity was produced on the second day in all cassava starch concentrations. However, the highest reducing sugar concentration was discovered in the 6% w/v cassava starch concentration. Thus, a batch submerged fermentation for carotenoid production by R. paludigenum was performed using the hydrolysate as the sole substrate. At the end of the fermentation, the total carotenoid was extracted, and the concentration was determined using spectrophotometry. The total yield of xanthophyll over biomass was higher than that of β-carotene. These findings elucidated the potency of cassava starch hydrolysate obtained from the starch hydrolyzed by B. subtilis, for carotenoid production by the red yeast R. paludigenum.
The Use of Selected Biomarkers, Phagocytic and Cholinesterase Activity to Detect the Effects of Dimethoate on Marine Mussel (Mytilus edulis) KHUSNUL YAQIN; BIBIANA WIDIATI LAY; ETTY RIANI
HAYATI Journal of Biosciences Vol. 15 No. 1 (2008): March 2008
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (70.987 KB) | DOI: 10.4308/hjb.15.1.32

Abstract

Effects of organophosphorous pesticide, dimethoate on blue mussels, Mytilus edulis using selected biomarkers have been studied. Mussels were exposed to serial dilutions of dimethoate, 7.88, 15.75, 31.35, and 63.00 µg/l including positive and negative controls for 14 days. The suppression effects of dimethoate on phagocytic activity significantly occurred at two lowest concentrations of dimethoate (7.88 and 15.75 µg/l), but stimulation effects significantly emerged at the following highest concentrations (31.35 and 63.00 µg/l). The declining tendency of the cholinesterase (ChE) activity (23% lower than the control) appeared when mussels exposed to 7.88 and 15.75 µg/l dimethoate. Moreover, the significant inhibition of the ChE activity occurred at 31.35 µg/l dimethoate exposure. This study suggested that the phagocytic and the ChE activity are useful biomarkers for assessing the affects of organophosporous pesticide, dimethoate on neuro-immune system of blue mussels, M. edulis. Key words: dimethoate, cholinesterase, phagocytic, blue mussels
Pengaruh pemberian vitamin C dan Vitamin E terhadap tantangan E. Coli Patogen pada ayam Pedaging I Wayan Batan; Bibiana W. Lay; Aisjiah Girinda; Hernomoadi Hoeminto
Hemera Zoa Vol. 76 No. 1 (1993): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

This study was undertaken to asses the effects of dietary vitamin C, vitamin E, vitamin C and vitamin E, combined with E. coli bacterin against patogenic E. coli challenge in broiler chickens.The completely randomized design was used, in 4 x 2 factorialexperiment i.e. consisted 4 vitamins and two bacterins. Each treatment combination had 10 chicks as replication, hence 80 broiler chicks were used in this study. Data obtained from the study were analysed using analysis of variance or Kruskal Wallis test in order to identify the influence of treatment factor and their interactions.The chickens were fed standard ration supplemented with 300 mg vitamin E or 330 mg vitamin C or 300 mg vitamin E and 330 mg vitamin C or no vitamin supplement per kg ration as control.E. coli bacterin inactivated by heat, was given to the chickens when they were 2 weeks old. The bacterin was injected directly to the airsacs of the treatment groups.At 4 weeks of age, all of the chickens were challenged with patogenic E. coli given directly to the airsacs.The data showed that the supplemental of either vitamin E, vitamin C, or vitamin E and vitamin C significantly reduced pathological lesions caused by E. coli challenge, whilst the E. colli bacterin alone did not. Suplemental vitamin C, or vitamin C and vitamin E, and also injection of E. coli bacterin significantly reduced chicken body weight.
Perbandingan pengaruh Escherichia coli dan vaksinasi ND La Sota terhadap bursa fabricus anak ayam pedaging Ekowati Handharyani; Hernomoadi .; Bibiana W. Lay; M.B. Malole .
Hemera Zoa Vol. 77 No. 2 (1995): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Infeksi E. coli pada anak ayam pedaging secara mandiri dapat menimbulkan penurunan jumlah sel limfosit (24.657%). radang interstisial (15.330%), hiperemi (24.811%). serta penurunan berat relatif bursa Fabricius (0.152 gram persen). Vaksinasi ND La Sota (tetes mata). dilanjutkan dengan infeksi E. coli menyebabkan penurunan jumlah sel limfosit (7.314%), edema radang (3.826%). hiperemi (9.569%) dan penurunan berat relatif bursa (0.150 gram persen). Pemberian vaksin ND La Sota (tetes mata) menyebabkan perubahan yang minimal pada bursa Fabricius berupa penurunan jumlah sel limfosit (2.03 1 %), edema (2.348%). hiperemi (2.03 1 %).
Kasus Kolera Pada Itik Wiwin Winarsih; Sugyo Hastowo; Bibiana W. Lay
Media Veteriner Vol. 4 No. 1 (1997): Media Veteriner
Publisher : Media Veteriner

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Abstract

Telah ditemukan kasus kolera pada itik yang diperiksa di Laboratorium Patologi Unggas, Fakultas Kedokteran Hewan, lnstitut Pertanian Bogor antara tahun 1992 hingga 1994.Kebanyakan itik terserang kolera yang akut, tanpa gejala klinis dan kematian mendadak. Beberapa itik terserang secara kronis dengan gejala klinis sesak nafas dan kebengkakan pada gejala kepala.Gambaran patologi anatonli yang tampak berupa titik perdarahan pada epikardium enteritis kartarhalis, hepatitis nekrotikan rqilier, linlpa membengkak, peradangan pada kantung udara, pecah kuning telur, serta peradangan pada ovariurn dan sinus. Dari darah jantung dan sumsum tulang itik dapat diisolasi bakteri Pasteureulla multocrda. Kelinci yang disuntik dengan suspensi bakteri mati dengan gejala septikemia yang ditandai dengan perdarahan padaepikardium dan selaput serosa. hiperemi dan pembengkakan umum serta enteritis.
Hemorrhagic Septicaemia In Alabio Ducks Infected With Pasteurella multocida By Various Routes Wiwin Winarsih; Hermonoadi Huminto; Bibiana Widiyati Lay; Ramdani Ramdani
Media Veteriner Vol. 7 No. 1 (2000): Media Veteriner
Publisher : Media Veteriner

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Abstract

Seratus ekor itik Alabio yang berumur lima rninggu dibagi menjadi empat kelompok berdasarkan rute infeksi yaitu intravena (i.v.), intramuskular (i.m.) intratrakhea (i.t.) dan kontrol (K). Kelompok perlakuan diinfeksi dengan bakteri P. multocida yang diisolasi dari itik dengan dosis 2x 105   cfu mL. Semua itik dinekropsi setelah satu, tiga, enam, 12 dan 24 jam infeksi. Itik-itik dari Kelompok i.v. menunjukkan septikemia hemorhagika, sedang itik dari Kelompok i.m. dan i.t. menunjukkan kerusakan yang sama tiga jam p.i. Semakin lama waktu pengamatan, semakin parah proses kerusakannya. Pada Kelompok Kontrol tidak ditemukan kerusakan
A laboratory scale research to obtain environmentally safe effluent from Final Waste Disposal Site leachate in Galuga owned by Regional Government of Bogor City was conducted from July 2006 through to April 2007. The experiment of production tolerable threshold effluent was carried out by aerating the leachate in 4 different aeration rates (0, 10, 30 and 70 liters/minute).  The research found that the most effective method to reduce pollutant was aerating at the rate of 70 liters/minutes.  Howev Nurhasanah .; Latifah K. Darusman; Surjono Hadi Sutjahjo; Bibiana Widiati Lay
Forum Pasca Sarjana Vol. 34 No. 1 (2011): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Abstract

A laboratory scale research to obtain environmentally safe effluent from Final Waste Disposal Site leachate in Galuga owned by Regional Government of Bogor City was conducted from July 2006 through to April 2007. The experiment of production tolerable threshold effluent was carried out by aerating the leachate in 4 different aeration rates (0, 10, 30 and 70 liters/minute).  The research found that the most effective method to reduce pollutant was aerating at the rate of 70 liters/minutes.  However, only some of pollutant (Cu, Zn, Pb and E.coli) can be reduced until below tolerable threshold.   Keywords: effluent, leachate, tolerable threshold
POTENSI NETRALISASI IMUNOGLOBULIN Y ANTITETANUS YANG DIISOLASI DARI TELUR AYAM (THE POTENCY NETRALIZATION OF ANTI TETANUS IMMUNOGLOBULIN Y THAT WERE ISOLATED FROM CHICKEN EGGS) I Nyoman Suartha; I Wayan Teguh Wibawan; Retno Damayanti Soejoedono; Bibiana W. Lay
Jurnal Veteriner Vol 8 No 2 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The porpuse of study was to explore the potential use of? anti tetanus IgY from eggs yolk as a substitute for anti tetanus serum raised in ?horses. The eggs were collected from chickens which have previously been immunized with tetanus toxoid. Neutralization potency test of anti tetanus IgY determined by ?Spearman-Karber method.? The highest mean titer of anti tetanus of egg yolk was 80.16 ? 33.55 IU/ml and the lowest was 1.69 ? 0.63 IU/ml. The concentration? of purified IgY was 1.644 ? 0.424 mg/ml. Spearman-Karber value of potency of anti tetanus IgY are 35 IU/ml. ?This research concluded that Chickens was capable of produced of anti tetanus in eggs yolk with value of potency are 35 IU/ml.