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Pengaruh Pengasaman terhadap Fotodegradasi Klorofil a Aji Wahyu Budiyanto; Soenarto Notosudarmo; Leenawaty Limantara
Jurnal Matematika & Sains Vol 13, No 3 (2008)
Publisher : Institut Teknologi Bandung

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Abstract

Chlorophyll is tetrapyrole pigment, which is potential to be developed as photosensitizer agent on cancer photodynamictherapy. Its environments and properties during interaction are an important parameter to know the next fate of thesensitizer. Acidic environment is one of the key factors on cancerous patients. The research was carried out to determinethe acidification effect against photostability of chlorophyll a that was observed by its spectral pattern. Result showedthat titration of less than 250 μL acetic acid 3 M caused degradation of chlorophyll a more rapidly. Volume and type ofacid properties did not only influenced pheophytinization reaction, but also caused pheophytin a to deaggregate, thatwas characterized by hypsochromic shift of Qy band.
KOMPOSISI DAN KANDUNGAN PIGMEN UTAMA TUMBUHAN TALIPUTRI Cuscuta australis R.Br. DAN Cassytha filiformis L. Heriyanto .; Leenawaty Limantara
Makara Journal of Science Vol 10, No 2 (2006): November
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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The Composition and The Content of The Main Pigments on Dodders Plant Cuscuta australis R.Br. and Cassytha filiformis L. Research on dodders plant Cuscuta australis R.Br. and Cassytha filiformis L was done to analyze their pigment composition and content. The pigment composition was analyzed by the use of thin layer chromatography (TLC) method based on spot color and retardation factor. The water content was measured according to Sudarmadji et. al. The chlorophylls and carotenoid contents were calculated by Porra et. al. and Gross equation, respectively. Result showed that Cuscuta australis R.Br. (green yellowish and orange) and Cassytha filiformis L. (green and brown reddish) had similar pigment composition consist of carotene, pheophytin a, chlorophyll a, chlorophyll b and xanthophyll. The average of the chlorophyll content from the highest to the lowest one was Cassytha filiformis L. green followed by Cuscuta australis R.Br. green yellowish and orange, while the total chlorophyll of Cassytha filiformis L. brown reddish was relatively similar with other dodders plant. The average of the carotenoid content from the highest to the lowest was Cuscuta australis R.Br. orange followed by Cuscuta australis R.Br. green yellowish. The pigment content of Cassytha filiformis L. was relatively similar to Cassytha filiformis L. brown reddish. Keywords: dodders plant, chlorophyll, carotenoid, pigment.
Efek Beta Karoten dan Agregasi Klorofil Pada Fotostabilitas Klorofil a dalam Pelarut Aseton Costa, Junet. F. da; Karwur, Ferry F; Limantara, Leenawaty
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.132 KB) | DOI: 10.31258/jnat.11.2.115-123

Abstract

Chlorophyll as photosynthetic pigment has many benefits to human such as antioxidant, antibacterial, color additive,immunity, and photosensitizer. Concerning to these applications, chlorophyll a will react with oxygen and light andreduce its effectiveness. It is then important to understand the stability of chlorophyll a on oxygen and light.Stability of chlorophyll a in the presence of beta-carotene (1:1) in acetone was studied for various waterconcentrations. Each solution was exposed to red light (ë e” 630 nm) for 0.5; 1.0; 2.5; 5.0; 10; 20; 30; 40; 50; and 60minutes and their spectrum were analysed. When water was added (8.33; 16.67; 25; 33.33; 41.67; 50; 58.33; 66.67;75; and 83.33 percent) to the acetone solution of chlorophyll a, the chlorophylls aggregate, and in the presence ofbeta-carotene, the chlorophyll more stable. The water shifts the chlorophyll a spectrum toward red duringillumination with or without the presence of carotene. The formation of oligomeric chlorophyll a aggregate occurswithin 10-20 minutes after the water was applied. This strategy of aggregating the chlorophylls to dissipateexcess energy captured from light does not support the photostability of chlorophyll as much as beta-carotene.Surprisingly, when beta carotene was mixed with 66.7% and 75.0% of water, the chlorophyll a degraded 5.56% and9.71% respectively. In conclusion, the aggregate form of chlorophyll a and the presence of beta-carotene increasephotostability of chlorophyll a in acetone solution.
Fotoproteksi Kurkumin terhadap β-Karoten pada Berbagai Nisbah Molar serta Aktivitas Antioksidannya Natalina, Elly; Rahayu, Puji; Sulistyowati, Sulistyowati; Limantara, Leenawaty
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

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Curcumin and β-carotene are two kinds of pigments serve as antitumor, anticancer and antioxidant agents. Theantioxidant activity of curcumin is stronger and more stable as compared to β-carotene, so it can protect β-carotene degradation from light and oxygen. Therefore curcumin has higher antioxidant activity as well. Theresearch was conducted by mixing curcumin and β-carotene in several molarity ratios followed by iradiation usingvolpi lamp for 0, 10, 30, 60, and 90 minutes. The result shows that curcumin has photoprotection effect because itcan protect photooxidation of β-carotene. Mixing of curcumin and β-carotene 1:1 was the most stable ratio forprotecting β-carotene.
Analisis Komposisi dan Kandungan Karotenoid Total dan Vitamin A Fraksi Cair dan Padat Minyak Sawit Kasar (CPO) Menggunakan KCKT Detektor PDA Syahputra, M. Rio; Karwur, Ferry F; Limantara, Leenawaty
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (684.953 KB) | DOI: 10.31258/jnat.10.2.89-97

Abstract

This study was carried out on two phases of Crude Palm Oil (CPO) to determine the total and composition ofcarotenoid and vitamin A content. Total of carotenoid was analyzed using spectrophotometer UV-Vis, and then theresult was calculated by Gross (1991) equation. The vitamin A content was calculated by NAS-NRC equation (1974).The type and composition of both phases of CPO were determined by Choo’s method (1994) by using HPLC withPhoto Diode Array (PDA) detector. The sample was prepared in two methods, with and without saponification. Theresult shows that total carotenoids in liquid and solid phase of CPO are 536 ± 13.2 g/g (liquid), 352 ± 17.7 μg/g(solid) and the vitamin A were 89.4 ± 2.2 RE (liquid), 58.7 ± 3.0 RE (solid), respectively. The carotenoid compositionsof both phases of CPO were dominated by - and -carotenes. The result shows that - and -carotenes preparedby saponification method in liquid phase are 29.03% and 60.88%, and without saponification (direct method) are28.14% and 59.44%. The result for solid phase shows that - and -carotenes by saponification are 25.89% and60.81%, and without saponification (direct method) are 30.00% and 56.92%. The research also shows the advantagesof using HPLC with PDA detector for identification and analysis of type and carotenoid composition.
ENKAPSULASI DENGAN KITOSAN-NATRIUM ALGINAT MENGGUNAKAN TEKNIK SPRAY-DRYING DAN FREEZE-DRYING Mulyadi, Naomi Megananda; Widyaningsih, Tri Dewanti; Wijayanti, Novita; Indrawati, Renny; Limantara, Leenawaty
Jurnal Pangan dan Agroindustri Vol 4, No 2 (2016)
Publisher : Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Brawijaya

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Abstract

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Perkembangan Sensitizer pada Terapi Fotodinamika Tumor dan Kanker Hingga Penuntunan Nanopartikel (Nanoparticulate Targeting) Dengan Antibodi Monoklonal INDRAWATI, RENNY; KARWUR, FERRY F; LIMANTARA, LEENAWATY
Indonesian Journal of Cancer Vol 4, No 3 (2010): Jul - Sep 2010
Publisher : "Dharmais" Cancer Center Hospital

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World Health Organization (WHO) estimates both the number of patients and mortality rates due to cancer will continue to rise. Various researches were conducted in order to prevent and handle the cases of tumors and cancers, including the application of photosynthetic pigment molecules known as photodynamic therapy (PDT). Chlorophylls, the main pigment in photosynthesis, have an ability to capture light energy and control series of photobiology and photochemical processes1. In PDT, chlorophyll or its derivatives compounds act as the sensitizer which have energy excitation by light radiation (visible or near infra red), and generate some reactive oxygen species which triggers the death of cancer cells selectively (through apoptosis and / or necrosis pathway)2. Sensitizer compounds have been progressing from the first to third generation. The development of the third generation sensitizer was influenced by the advances of nanotechnology which lead to the improvement of PDT efficacy. The structure and size of nanoparticles can increase light absorption, and make the sensitizer accumulate in cancer tissues more specifically 3-4. Furthermore, nanoparticulatte targeting also interested to be studied because by conjugate functional groups, i.e. monoclonal antibody, on the sensitizer, it can improve the selectivity of therapy in targeting tumor and cancer tissues.
Pigmen Spirulina sebagai Senyawa Antikanker Pirenantyo, Prasetyandaru; Limantara, Leenawaty
Indonesian Journal of Cancer Vol 2, No 4 (2008): Oct - Dec 2008
Publisher : "Dharmais" Cancer Center Hospital

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Spirulina, ganggang hijau biru yang berbentuk spiral, mengandung fitonutrisi yang cukup lengkap dan berpotensi sebagai agen antikanker yang efektif. Fikosianin, klorofil a, dan karotenoid adalah pigmen-pigmen yang dikandung Spirulina. Artikel ini merangkum berbagai penelitian in vivo dan ex vivo yang melaporkan ketiga pigmen tersebut merupakan antioksidan kuat. Bahkan, beberapa laporan mencatat bahwa turunan klorofil a juga berpotensi sebagai antimutagen, induktor apoptosis, dan fotosensitizer untuk PDT (Fotodinamika Terapi Kanker). PDT diharapkan memberikan harapan baru dalam terapi kanker. Namun demikian, tidak hanya pigmen yang berperan sebagai antioksidan dalam terapi penyembuhan kanker dengan Spirulina, nutrisi lain seperti asam lemak GLA (gamma linoleic acid), enzim superoksida dismutase (SOD), dan oligosakarida juga turut berperan sebagai senyawa antikanker. Spirulina dijual dalam bentuk suplemen golongan CAM (Complementary and Aternative Medicine). Terapi dengan mengkonsumsi spirulina cair, tablet, kapsul, ataupun bubuk secara teratur menawarkan sebuah jawaban bagi mereka yang sedang bergumul dengan kanker stadium awal jika ingin menghindari pembedahan, radioterapi, ataupun kemoterapi.vKata kunci: spirulina, fikosianin, klorofil a, karotenoid, antioksidan, complementary and alternative medicine
Potensi Astaxantin sebagai Senyawa Antikanker Pratiwi, Reny; Limantara, Leenawaty
Indonesian Journal of Cancer Vol 2, No 4 (2008): Oct - Dec 2008
Publisher : "Dharmais" Cancer Center Hospital

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Astaxantin merupakan salah satu golongan karotenoid xantofil yang memiliki peran penting dalam menjaga kesehatan tubuh. Pada berbagai studi telah dibuktikan peran astaxantin dalam melindungi sel dan jaringan dari kerusakan terutama akibat stres oksidatif yang juga dapat memicu terjadinya kanker. Dalam tulisan ini akan dibahas mengenai potensi astaxantin sebagai senyawa antikanker.Kata Kunci: astaxantin, antioksidan, antikanker.
KARAKTERISASI ANTOSIANIN BUAH MURBEI SPESIES MORUS ALBA DAN MORUS CATHAYANA DI INDONESIA Sitepu, Rehmadanta; Heryanto, Heryanto; Brotosudarmo, Tatas H.P.; Limantara, Leenawaty
Natural Science: Journal of Science and Technology Vol 5, No 2 (2016): Volume 5 Number 2 (August 2016)
Publisher : Univ. Tadulako

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Buah murbei kaya antioksidan karena kandungan antosianin yang banyak. Sayangnya,  pemanfaatannya sebagai sumber antioksidan di Indonesia masih  minim. Pemanfaatan tanaman murbei hanya sebatas daun yang dijadikan pakan ternak dan teh. Penelitian ini bertujuan untuk melakukan karakterisasi antosianin buah murbei spesies Morus alba dan Morus cathayana. Karakterisasi antosianin ekstrak buah murbei Morus cathayana dan Morus alba dilakukan menggunakan pelarut 0,1% asam klorida (HCl)  dalam metanol dan dianalisis menggunakan spektrofotometer UV-tampak dan Kromatografi Cair Kinerja Tinggi (KCKT). Hasil serapan spektra UV-tampak yang diperoleh dari ekstrak kasar menunjukkan spektra serapan ekstrak buah M. cathayana memberikan nilai yang lebih tinggi ± 1,3 a.u dibandingkan serapan yang diperoleh dari ekstrak kasar buah M. alba dengan serapan ± 0,4 a.u. Nilai total antosianin yang diperoleh dari M. cathayana sebanding dengan serapan ekstrak segarnya, yaitu 40,39 ± 7,64 mg/g berat kering dibandingkan  M. alba yang memiliki nilai total antosianin 11,57 ± 3,02 mg/g berat kering. Hasil kromatogram dari KCKT menggunakan kolom XR-ODS dengan pengelusi A adalah 0,1% asam formiat dalam asetonitril dan pengelusi B adalah 0,1% asam formiat dalam air menunjukkan baik M. cathayana maupun M. alba memiliki dua pigmen antosianin dominan. Walaupun bergitu, intensitas kromatogram M. cathayana lebih tinggi dibandingkan intesitas pada M. alba. Nilai intensitas M. cathayana adalah 50 mAU untuk waktu tambat 7,86 menit dan 15 mAU untuk waktu tambat 8,38 menit. Nilai intensitas M. alba adalah 10 mAU untuk waktu tambat 7,35 menit dan 3 mAU untuk waktu tambat 7,76 menit. Dua antosianin dominan pada M. alba dan M. cathayana  diprediksi  merupakan sianidin-3-O-glukosida dan sianidin-3-O-rutinosida.
Co-Authors A. B. Susanto A.B. SUSANTO A.B. Susanto AB Susanto Adhiwibawa, Marcelinus A.S. Agus Sabdono Aji Wahyu Budiyanto Amelia Myristi Lolita Bambang Cahyono Budi Prasetyo C Hanny Wijaya Christina Astutiningsih Dece Elisabeth Sahertian Diah Mustika Lukitasari Diah Mustika Lukitasari, Diah Mustika Dian Resita Dian Ridwan Nurdiana Dwi I Prayitmo DWI SUSILANINGSIH EDWIN MAHENDRA Elly Natalina Endrika Widyastuti Ferdiansyah Ferdiansyah FERRY F KARWUR Ferry Fredy Karwur Ferry Fredy Karwur Gabriela Johana Septiany, Gabriela Johana Hana Susanti Maleta Hari Kristopo Helly de Fretes Heriyanto . Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heryanto Heryanto Hong, Khoo Mun Indriatmoko Indriatmoko Junet. F. da Costa Katarina Purnomo Salim Lia Kusmita M. Rio Syahputra Madalena Madalena Maria Ika Putri Soegiarto, Maria Ika Putri Muhammad Diah Khoirul Huda Naely K Wusqy NAELY K. WUSQY Naomi Megananda Mulyadi, Naomi Megananda Neltji Herlina Ati Novita Wijayanti Ocky K Radjasa Ocky K. Radjasa Ocky Karna Radjasa Panca, Ida Nur Prasetyandaru Pirenantyo, Prasetyandaru Puji Rahayu Puji Rahayu Rebecca Christiana Reni Subawati Kusumaningtyas Renny Indrawati Renny Indrawati Renny Indrawati Renny Indrawati Renny Indrawati, Renny Reny Pratiwi, Reny Rosita Dwi Chandra Rosita Dwi Chandra, Rosita Dwi Salim, Katarina Purnomo Sitepu, Rehmadanta Soenarto Notosoedarmo Soenarto Notosudarmo Sulistyowati Sulistyowati Suryasatriya Trihandaru Susanti Pudji Hastuti Tatas H.P. Brotosudarmo Tatas H.P. Brotosudarmo Tatas Hardo Panintingjati Brotosudarmo Tri Dewanti Widyaningsih Venny Santosa Widya Dwi Rukmi Putri Windu Merdekawati Yundari, Yundari _ Heriyanto