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OPTIMASI NUTRISI MEDIA PERTUMBUHAN BAKTERI TERMOFIL PENGHASIL SELULASE DARI SUMBER AIR PANAS RIMBO PANTI -, Armaini; Dharma, Abdi; Syukur, Sumaryati; -, Jamsari
Jurnal Riset Kimia Vol 5, No 1 (2011): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i1.165

Abstract

 Optimization have been done on the media for the growth of the isolated thermophiles bacteria from hot springs Rimbo Panti, the nutrients comprising variety of carbon sources such as CMC (carboxymethyl cellulose), avicell (micro crystalline cellulose), and cellobiose, with a variety of sources organic nitrogen, peptone, extracts yeast, tryptone, and urea, as well as variations consist of inorganic nitrogen sources, KNO3, NaNO3, (NH4)2SO4, and (NH4)NO3. Determination of cellulase activity performed using DNS reagent (3,5-dinitro salicylic acid). Maximum cellulase production with high activity based on the results of this research, the best of carbon source is CMC with optimum concentration 0.125%, inorganic nitrogen source is peptone with the optimum concentration of 0.3 to 0.4% and the inorganic nitrogen source is (NH4)2SO4 with optimum concentration of 0.2 - 0.25%. Optimization of size of inoculums obtained the optimum amount of inoculums 2%. Keywords: Optimization, thermophiles bacteria, cellulose, carbon sources, nitrogen sources
OPTIMASI NUTRISI MEDIA PERTUMBUHAN BAKTERI TERMOFIL PENGHASIL SELULASE DARI SUMBER AIR PANAS RIMBO PANTI Armaini -; Abdi Dharma; Sumaryati Syukur; Jamsari -
Jurnal Riset Kimia Vol. 5 No. 1 (2011): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i1.165

Abstract

 Optimization have been done on the media for the growth of the isolated thermophiles bacteria from hot springs Rimbo Panti, the nutrients comprising variety of carbon sources such as CMC (carboxymethyl cellulose), avicell (micro crystalline cellulose), and cellobiose, with a variety of sources organic nitrogen, peptone, extracts yeast, tryptone, and urea, as well as variations consist of inorganic nitrogen sources, KNO3, NaNO3, (NH4)2SO4, and (NH4)NO3. Determination of cellulase activity performed using DNS reagent (3,5-dinitro salicylic acid). Maximum cellulase production with high activity based on the results of this research, the best of carbon source is CMC with optimum concentration 0.125%, inorganic nitrogen source is peptone with the optimum concentration of 0.3 to 0.4% and the inorganic nitrogen source is (NH4)2SO4 with optimum concentration of 0.2 - 0.25%. Optimization of size of inoculums obtained the optimum amount of inoculums 2%. Keywords: Optimization, thermophiles bacteria, cellulose, carbon sources, nitrogen sources
Penapisan, isolasi, dan karakterisasi mikroalga yang berpotensi sebagai sumber biodiesel dari perairan Danau Kerinci, Jambi Riska Hernandi; Abdi Dharma; A Armaini
Jurnal Litbang Industri Vol 9, No 1 (2019)
Publisher : Institution for Industrial Research and Standardization of Industry - Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (506.968 KB) | DOI: 10.24960/jli.v9i1.4326.41-49

Abstract

Mikroalga menjadi sumber minyak nabati yang berpotensi sebagai bahan baku pembuatan biodiesel dengan kandungan lipid 30-70% dari berat biomassa kering dan komposisi asam lemak yang lengkap. Penelitian ini bertujuan untuk mempelajari keragaman spesies mikroalga dari perairan Danau Kerinci di Jambi, menganalisis kandungan lipid dan asam lemak isolat mikroalga. Mikroalga diisolasi dengan kombinasi teknik goresan, pengenceran berseri, dan mikropipet. Penentuan tingkat pertumbuhan dengan spektrofotometer UV-Vis. Penentuan berat biomassa kering secara gravimetri. Analisis kualitatif lipid dengan uji nile red menggunakan mikroskop flourescence. Analisis kuantitatif lipid dengan ekstraksi menggunakan n-heksana.  Analisis kandungan asam lemak dengan alat GC-MS. Terdapat 19 spesies mikroalga yang diidentifikasi dan 2 spesies berhasil ditapis dengan stres salinitas, yaitu MA1 (Scenedesmus rubescens) dan MA2 (Galdieria sulphuraria). MAI dan MA2 memiliki kandungan lipid yang lebih tinggi pada pupuk Growmore dibandingkan pada medium Bold’s Basal. MA1 memiliki kandungan lipid 31,95% pada medium Bold’s Basal dan 32,4% pada pupuk Growmore. MA2 memiliki kandungan lipid 28,72% pada medium Bold’s Basal dan 28,93% pada pupuk Growmore. Mikroalga MA1 dan MA2 dapat dijadikan sumber biodiesel dengan kandungan lipid dan asam lemak jenuh (C16:0, C18:0) yang tinggi.ABSTRACTMicroalgae has been considered recently as a promising biomass feedstock with great potential for biodiesel production with 30-70% lipid content of the dry biomass weight and produces high fatty acid. This research investigated the diversity of microalgae species from waters of Lake Kerinci in Jambi and analysis of the lipid content and fatty acid of microalgae. The isolation was done by agar plate, serial dilution, and micropipette method. The growth rate of the isolated microalgae was determined by UV-Vis spectrophotometer. Dry biomass weight was determined gravimetrically. Nile red staining performed on the isolates to observe the potential of lipid content. Lipids were extracted using n-hexane. Fatty acid analysis by GC-MS. From the results of identification, there were 19 species of microalgae and 2 species were screened with salinity stress. Based on identification of the both isolates, it is known that MA1 isolate is Scenedesmus rubescens and MA2 is Galdieria sulphuraria. MAI and MA2 had higher lipid content in Growmore agrolyzer than Bold’s Basal medium. MA1 had lipid content 31.95% in Bold’s Basal medium and 32.4% in Growmore agrolyzer, MA2 had lipid content 28.72% in Bold’s Basal medium and 28.93 % in Growmore agrolyzer. MA1 and MA2 was a potential as a biodiesel source with high lipid content and saturated fatty acids (C16:0, C18:0).
Penapisan, isolasi, dan karakterisasi mikroalga yang berpotensi sebagai sumber biodiesel dari perairan Danau Kerinci, Jambi Riska Hernandi; Abdi Dharma; A Armaini
Jurnal Litbang Industri Vol 9, No 1 (2019)
Publisher : Institution for Industrial Research and Standardization of Industry - Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (506.968 KB) | DOI: 10.24960/jli.v9i1.4326.41-49

Abstract

Mikroalga menjadi sumber minyak nabati yang berpotensi sebagai bahan baku pembuatan biodiesel dengan kandungan lipid 30-70% dari berat biomassa kering dan komposisi asam lemak yang lengkap. Penelitian ini bertujuan untuk mempelajari keragaman spesies mikroalga dari perairan Danau Kerinci di Jambi, menganalisis kandungan lipid dan asam lemak isolat mikroalga. Mikroalga diisolasi dengan kombinasi teknik goresan, pengenceran berseri, dan mikropipet. Penentuan tingkat pertumbuhan dengan spektrofotometer UV-Vis. Penentuan berat biomassa kering secara gravimetri. Analisis kualitatif lipid dengan uji nile red menggunakan mikroskop flourescence. Analisis kuantitatif lipid dengan ekstraksi menggunakan n-heksana.  Analisis kandungan asam lemak dengan alat GC-MS. Terdapat 19 spesies mikroalga yang diidentifikasi dan 2 spesies berhasil ditapis dengan stres salinitas, yaitu MA1 (Scenedesmus rubescens) dan MA2 (Galdieria sulphuraria). MAI dan MA2 memiliki kandungan lipid yang lebih tinggi pada pupuk Growmore dibandingkan pada medium Bold’s Basal. MA1 memiliki kandungan lipid 31,95% pada medium Bold’s Basal dan 32,4% pada pupuk Growmore. MA2 memiliki kandungan lipid 28,72% pada medium Bold’s Basal dan 28,93% pada pupuk Growmore. Mikroalga MA1 dan MA2 dapat dijadikan sumber biodiesel dengan kandungan lipid dan asam lemak jenuh (C16:0, C18:0) yang tinggi.ABSTRACTMicroalgae has been considered recently as a promising biomass feedstock with great potential for biodiesel production with 30-70% lipid content of the dry biomass weight and produces high fatty acid. This research investigated the diversity of microalgae species from waters of Lake Kerinci in Jambi and analysis of the lipid content and fatty acid of microalgae. The isolation was done by agar plate, serial dilution, and micropipette method. The growth rate of the isolated microalgae was determined by UV-Vis spectrophotometer. Dry biomass weight was determined gravimetrically. Nile red staining performed on the isolates to observe the potential of lipid content. Lipids were extracted using n-hexane. Fatty acid analysis by GC-MS. From the results of identification, there were 19 species of microalgae and 2 species were screened with salinity stress. Based on identification of the both isolates, it is known that MA1 isolate is Scenedesmus rubescens and MA2 is Galdieria sulphuraria. MAI and MA2 had higher lipid content in Growmore agrolyzer than Bold’s Basal medium. MA1 had lipid content 31.95% in Bold’s Basal medium and 32.4% in Growmore agrolyzer, MA2 had lipid content 28.72% in Bold’s Basal medium and 28.93 % in Growmore agrolyzer. MA1 and MA2 was a potential as a biodiesel source with high lipid content and saturated fatty acids (C16:0, C18:0).
EFEKTIFITAS BAKTERI UNTUK DEGRADASI SAMPAH PLASTIK YANG DIISOLASI DARI TEMPAT PEMBUANGAN AKHIR (TPA) AIR DINGIN PADANG Nessa Satya Wati; Armaini Armaini; Tedy Alfajri; Indriani Sahira
JURNAL KIMIA SAINTEK DAN PENDIDIKAN Vol 5 No 2 (2021): JURNAL KIMIA SAINTEK DAN PENDIDIKAN
Publisher : Program Studi Kimia - Universitas Sari Mutiara Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.739 KB)

Abstract

The problem of waste in Indonesia, which has not been handled properly, is plastic waste, where the high total consumption of plastic causes the amount of plastic waste produced to continue to increase every year. This study aims to determine the bacteria that can degrade plastic isolated from the Padang Cold Water Final Disposal Site (TPA). The method used in this study is that the sample was taken from the Padang Air Cold TPA at several points and then diluted. The bacteria were isolated in a petri dish that already contained NA (nutrient agar) media with the streak method, then incubated for 48 hours, the bacteria that have been grown are purified to get more specific bacteria, after being purified, degradation tests are carried out by inserting sterilized and weighed plastic pieces into a petri dish containing bacteria and NA media, the plastic samples used are LDPE (Low-Density Polyethylene) and Biodegradable. In the study 2 types of bacteria were purified , the weight reduction results in LDPE plastic samples were 10.61% on bacteria 1 and Biodegradable by 10.3% on bacteria 1. The SEM (Scanning Electron Microscope) test showed that the surface of the test sample was wavy, and there were wrinkles. In this study, it can be concluded that the isolated bacteria 1 can degrade plastic.
Evaluation of Lipid Profile and Liver Function After Administration of Scenedesmus dimorphus in Obese Mice Armaini Armaini; Siti Hajir; Yetria Rilda
Jurnal Riset Kimia Vol. 13 No. 2 (2022): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v13i2.503

Abstract

Obesity is a major public health problem in developing countries and is a significant risk factor for metabolic disorders. Microalgae Scenedesmus dimorphus (S. dimorphus) contains bioactive compounds such as pigment function as antioxidants, and omega-3 and omega-6 PUFAs have potential as nutraceuticals. The study aimed to evaluate the lipid profile and liver function after the administration of S. dimorphus in obese mice. The research design uses mice which are divided into 6 groups; Group 1 (G1) normal control, G2 control Obesity, G3 treatment with Orlistat, G4 treatment S. dimorphus (0.25mg/g BW), G5 treatment S. dimorphus (0.5 mg/g BW) and G6 treatment S. dimorphus (0.75 mg/g BW) each group consisted of 5 mice and 21 days of observation time. The parameters observed were lipid profile and liver function of mice. Based on the results of the study, the effective dose for treating obesity is a dose of S. dimorphus 0.75 mg/g (BW) can reduce cholesterol, triglycerides, and LDL levels, respectively 67.7 mg/dl, 49.2 mg/dl, 10 , 2 mg/dl, and increased HDL, 68.32 mg/dl compared to control of obesity (G2), respectively 108.7 mg/dl, 139.1 mg/dl, 20.6 mg/dl and HDL 60, 28 mg/dl, this dose is also effective for improving the function of blood pressure by reducing AST and ALT 15.6 U/L and 18.8 U/L, respectively, compared to the obesity group (G2), which is 26.6 U/L, and 29,7 U/L. Based on the results of the study it can be concluded that S. dimorphus is useful for anti-obesity for mice (Mus musculus).