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All Journal Indonesian Journal of Agricultural Science JURNAL BIOLOGI INDONESIA
P. Lestari
Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development Jalan Tentara Pelajar No. 3A, Bogor 16111, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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DEVELOPMENT AND APPLICATION OF 1536-PLEX SINGLE NUCLEOTIDE POLYMORPHISM MARKER CHIP FOR GENOME WIDE SCANNING OF INDONESIAN RICE GERMPLASM Utami, Dwinita W.; Rosdianti, I.; Lestari, P.; Satyawan, D.; Rijzaani, H.; Tasma, I M.
Indonesian Journal of Agricultural Science Vol 14, No 2 (2013): October 2013
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

A successful molecular breeding program requires detailed and comprehensive understanding of the diversity of rice germ-plasm and genetic base of target traits. The objective of this research was to develop the high throughput 1536-SNP chip linked to heading date and yield component traits and used it for genotyping the diverse Indonesian rice germplasm. The genotype data obtained could be used for diversity analysis and genome wide association mapping study. A 1536-SNP genome wide assay was developed using the Illumina’s GoldenGate technology. The SNP markers were selected in the rice genome regions containing heading date and yield component genes or regions where the quantitative trait loci (QTLs) of the two traits were mapped. The developed custom SNP chips were then used for genotyping 467 rice accessions showing diversity in heading dates and yield components. The assay can reliably be used for diversity analysis and mapping genes associated with heading date and yield component traits. For 1536-SNP BIO-RiceOPA-1 custom chip designed, a total of 34.832 SNPs distributed in rice genome particularly in the region of heading date and yield component genes or QTLs were identified. A total of 1536-SNP were selected and confirmed to be used for genotyping analysis. Analysis performance and quality of 1536-SNP BIO-RiceOPA1 showed that 60% (918/1536) of total SNP markers had a good differentiating power in scanning the rice accessions tested (MAF > 0.2). The 1536-SNP genome wide assay Illumina’s GoldenGate designed was useful for diversity analysis and could be used as SNP marker for large scale genotyping in rice molecular breeding involving Indica-Indica, Indica-Japonica and Indica-Tropical Japonica crosses.
Produktivitas Klon Katuk (Sauropus androgynus (L.) Merr.) Triploid pada Berbagai Kombinasi Perlakuan Pemupukan dan Waktu Pemangkasan Juhaeti, Titi; Lestari, P.; Utami, N W
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3161.361 KB) | DOI: 10.14203/jbi.v11i1.2158

Abstract

Ease of access, affordability and continuity of katuk production is important to support a variety of supplements industry-katuk based being developed at this time. The problem is the existing clones and its cultivation technique has not been able to fulfil the market needs. Therefore clone assembling was done to produce triploid katuk with larger leaves size. In this paper we put forward the research on the fertilization and pruning time that appropriate to support the leaf production of triploid katuk. The study was carried out at the experimental plot in Botany Division of Research Center for Biology-LIPI. The experimental designs was Nested RCBD Design, the main plot were 2 level of pruning time (P1: 3 Weeks After First Pruning (MSPP) and P2: 5 MSPP). The subplots were fertilization (K: Control, B: Beyonic, B ¼: Beyonic + NPK¼, B ½:  Beyonic+ ½ dose NPK and NPK: NPK without Beyonic). The growth parameters observed were plant height, number of leaves, the leaf size, were done every week until 8 weeks after planting (WAP). The first pruning to harvest shoots were done at 8 WAP, and the next pruning were done according pruning time treatment i.e. P1 and P2. The pruning was done 3 times. The variables observed at pruning time were shoot length, number of leaves, leaf size, the pruned fresh weight and the fresh weight of edible parts. The result indicated that the triploid katuk clones showed a good response to fertilization both growth and leaf production. The biofertilizers can be combined with the use of inorganic fertilizers to increase edible part of pruning harvest production. Keywords: pruning time, bioorganic fertilizer, triploid katuk, pruning harvest production 
PRODUKTIVITAS KLON KATUK (SAUROPUS ANDROGYNUS (L.) MERR.) TRIPLOID PADA BERBAGAI KOMBINASI PERLAKUAN PEMUPUKAN DAN WAKTU PEMANGKASAN Juhaeti, Titi; Lestari, P.; Utami, N W
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2158

Abstract

Ease of access, affordability and continuity of katuk production is important to support a variety of supplements industry-katuk based being developed at this time. The problem is the existing clones and its cultivation technique has not been able to fulfil the market needs. Therefore clone assembling was done to produce triploid katuk with larger leaves size. In this paper we put forward the research on the fertilization and pruning time that appropriate to support the leaf production of triploid katuk. The study was carried out at the experimental plot in Botany Division of Research Center for Biology-LIPI. The experimental designs was Nested RCBD Design, the main plot were 2 level of pruning time (P1: 3 Weeks After First Pruning (MSPP) and P2: 5 MSPP). The subplots were fertilization (K: Control, B: Beyonic, B ¼: Beyonic + NPK¼, B ½:  Beyonic+ ½ dose NPK and NPK: NPK without Beyonic). The growth parameters observed were plant height, number of leaves, the leaf size, were done every week until 8 weeks after planting (WAP). The first pruning to harvest shoots were done at 8 WAP, and the next pruning were done according pruning time treatment i.e. P1 and P2. The pruning was done 3 times. The variables observed at pruning time were shoot length, number of leaves, leaf size, the pruned fresh weight and the fresh weight of edible parts. The result indicated that the triploid katuk clones showed a good response to fertilization both growth and leaf production. The biofertilizers can be combined with the use of inorganic fertilizers to increase edible part of pruning harvest production. Keywords: pruning time, bioorganic fertilizer, triploid katuk, pruning harvest production 
Co-Authors D. Satyawan Dwinita W. Utami H. Rijzaani I M. Tasma I. Rosdianti TITI JUHAETI Utami, N W Utami, N W