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All Journal Jurnal Ilmu Ternak Jurnal Medik Veteriner Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary
Tita Damayanti Lestari
Department Of Reproduction, Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Pengujian Anti Protein Produksi Blastosis (Anti-PAG) melalui Metode Dot Blot (Evaluatin Of Anti PAG From Blastosis through Dot Blot Method) Tita Damayanti Lestari
Jurnal Ilmu Ternak Vol 11, No 1 (2011)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v11i1.407

Abstract

Penelitian  pengujian  reaksi antiprotein dan protein produksi blastosis dilakukan dengan tujuan mengetahui apakah  terjadi saling mengenali antara anti-PAG dan protein PAG yang terdapat dalam serum sapi bunting. Pengujian dilakukan dengan menggunakan metode Dot Blot. Uji dilakukan terhadap serum sapi bunting   umur 15, 20, 25 dan 30 hari, masing-masing dari empat ekor dengan dosis pengenceran anti-PAG adalah 1/100, 1/200 dan 1/400. Hasil reaksi berupa warna ungu dikonversikan kedalam angka menggunakan metode NIH Image Version 1.62. Data dianalisis menggunakan Rancangan Acak Lengkap Pola Tersarang, dilanjutkan dengan uji jarak berganda Duncan untuk mengetahui pengaruh serum dalam dosis. Uji statistik pada hasil reaksi Dot Blot menunjukkan perbedaan yang  signifikan antara serum sapi dara bunting 15,20,25 dan 30 hari dalam dosis pengenceran 1/100, 1/200 dan 1/400. PAG pada serum sapi dara bunting 15 hari mampu dikenali oleh anti-PAG pada pengenceran 1/400. Serum sapi dara bunting umur 30 hari memiliki nilai paling sensitif pada semua dosis pengenceran, disusul kemudian dengan serum sapi umur kebuntingan 25, 20 dan 15 hari. Kata Kunci : Anti-PAG, PAG, Dot Blot, serum  sapi bunting
Hubungan antara skor hasil bacaan heat detector dengan gambaran sitologi vagina sebagai parameter penentuan estrus pada kambing Nanda Irsa Meydilasari; Tatik Hernawati; Tita Damayanti Lestari; Erma Safitri; Trilas Sardjito; Ismudiono Ismudiono; Sri Hidanah
Ovozoa : Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (655.361 KB) | DOI: 10.20473/ovz.v9i1.2020.7-11

Abstract

This study aimed to determine the relationship between heat detector reading results and the feature of vaginal swab preparations as a parameter for determining estrus in Peranakan Etawa goats. Five healthy non-pregnant goats weighing 20-30 kg, were synchronized using PGF2α (7.5 mg Dinoprost, Enzaprost) intramuscularly twice with 11 days interval. Vaginal cytology examination began the day after the second PGF2α injection. The heat detector (Draminski®, Dramiński SA, Poland) is an estrus detecting device based on the amount of electric current flowing through the vaginal mucus. Spearman correlation analysis showed a relationship (p <0.05) between heat detector reading and estrus detection based on vaginal cytology. The heat detector readings were very significantly correlated positively (p <0.01) with the number of epithelial cells (coefficient correlation, r= 0.564), but negatively correlated with the number of cornified cells (r = –0.854), and also significantly correlated positively (p <0.05) to the number of leukocytes (r = 0.487). The estrus stages were significantly correlated positively (p <0.05) only to the number of leukocytes (r = 0.918). Likewise, the number of epithelial cells was highly correlated negatively (p <0.01) only to cornified cells (r = –0,643), and the number of cornified cells was significantly correlated negatively (p <0.05) only to the number of leukocytes (r = –0.472). It could be concluded that the features of the proestrus, estrus and metestrus stages based on the goat vaginal cytology was correlated with the reading results of the heat detector.
Relationship between the value of the estrous detector measurement result and serum progesterone level in Etawa crossbred goats after estrous synchronization Annisa Lita Triagil; Ismudiono Ismudiono; Tita Damayanti Lestari; Abdul Samik; Budi Utomo; Boedi Setiawan
Ovozoa : Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.82-85

Abstract

This study was conducted to determine the relationship between the estrous detector's reading value (Draminski®, Dramiński S.A, Poland) and serum progesterone levels on Etawa crossbred does. Eighteen healthynon-prehnant Etawa crossbreds does weighing 20-25 kg, in their first parity were used in this study. The does were divided equally into two groups. In the two groups, the does were synchronized their estrus by means of intramuscular and intravulvar injection of 7.5 mg prostaglandin Fɑ (PGF2ɑ) twice in 11-day intervals. After the second injection, the estrous detector value measurement, visual observation of the estrus sign, and blood sampling were conducted for five days. The blood sample was processed into the serum to examine for progesterone levels by ELISA. The data analysis was using the simple correlation method on SPSS type 23 for windows. The result showed that a positive relationship (p <0.05) with coefficient correlation (r) was 0.609 between the values of the estrous detector measurement with serum progesterone levels. It could be concluded that the estrous detector's low value indicates that the Etawa crossbred was in the estrus phase and followed by the decreasing of progesterone level but not reaching the basal level.
Detection of Fertility Levels of Female Bawean Deer (Axis kuhlii) Based on Fecal Steroid Metabolic Mitha Ardila Rahmawati; Mas’ud Hariadi; Tjuk Imam Restiadi; Rimayanti Rimayanti; Tita Damayanti Lestari; Pudji Srianto
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.84-90

Abstract

The aim of the study was to detect the fertility level of females Bawean deer (Axis kuhlii) through fecal steroid metabolic which were tested using Enzyme-Linked Immunosorbent Assay (ELISA). The study used five females Bawean deer in Taman Flora Surabaya, which were marked using R1, R2, R3, R4, and R5 necklaces. The feces of each deer was collected on the 1st day, 6th day, 11th day, 16th day, and 21st day. The samples which were collected then extracted using the freeze dry method. Samples were tested using ELISA. The results of the measurement of the levels of fecal steroid metabolic  in five Bawean deers showed that three Bawean deer (R1, R4, and R5) were in the luteal or pregnant phase as indicated by the increased of progesterone from 1st day to 21st day. While the other two Bawean deer (R2 and R3) were in the follicular or estrous phase as indicated by the decreased of level of faecal steroid metabolic  on 11th day for deer R2 and 16th day for deer R3. The results showed that the levels of the fecal steroid metabolic can be used to determine the follicular phase and luteal phase and the pregnancy status of female Bawean deer.
Identification of Cat (Felis catus) Blood Splatter on Cotton Fabric After Periods of Drying Using Leucomalachite Green and Takayama Reagent Vanessa Ann Charles; Tita Damayanti Lestari; Djoko Legowo; Ismudiono Ismudiono; Nove Hidajati; Retno Sri Wahyuni
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28593

Abstract

Blood-stain or blood splatter analysis when used properly can assist in establishing a chain of events linked to violent crimes (Bevel and Gardner, 2008). The methods used in detecting blood splatters in the field are chemical methods. Leucomalachite green is a colorimetric test which is used to test the presence of blood (Castro and Coyle, 2008). Takayama reagent is a confirmatory test for blood (Strassman, 1922). The aim of this research is to detect the blood splatter on cotton fabric after it has been dried for 1 day, 3 days and 5 days using Leucomalachite green and Takayama reagent. Cotton fabric was specifically chosen for this experiment with 3 different periods of drying. The unstained cotton fabric was cut into squares, and a blood sample was splattered on each piece. The fabrics splattered with blood were then dried for 1 day, 3 days and 5 days. The blood splatter was then tested using Leucomalachite green and Takayama reagent, and the results were noted afterwards. For the control, red food dye was dried for 1 day then tested with Leucomalachite green and Takayama reagent. The image results of the Leucomalachite green test are analyzed using ImageJ software 1.8.0_112 where the red, green and blue pixels are converted to grayscale. The image results of the Takayama test are graded based on the number and pattern of crystal. In conclusion, Leucomalachite green and Takayama reagent are able to detect cat blood splatter on the cotton fabric. Leucomalachite green produced a higher intensity/ darker colour as a result of an older sample, and the lower intensity/ lighter colour as a result of a fresher sample of the Leucomalachite green test. Takayama reagent produced a densely packed pattern of crystals as a result of an older sample, and the loosely packed pattern of crystals as a result of a fresher sample of the Takayama test.
Co-Authors Abdul Samik Annisa Lita Triagil Boedi Setiawan BUDI UTOMO Djoko Legowo ERMA SAFITRI I. Ismudiono Ismudiono Ismudiono Mas’ud Hariadi Mitha Ardila Rahmawati Nanda Irsa Meydilasari Nove Hidajati Pudji Srianto Restiadi, Tjuk Imam Retno Sri Wahyuni Rimayanti Rimayanti Sri Hidanah Tatik Hernawati Trilas Sardjito Vanessa Ann Charles