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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan Sainteks Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biotropika Indonesian Journal of Biotechnology JSDH ANNALES BOGORIENSES Marine Research in Indonesia Biosaintifika: Journal of Biology & Biology Education Menara Perkebunan Jurnal Tanah dan Iklim Makara Journal of Science
YULIN LESTARI
Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Veterinary

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Characterization of Ethanolic Extract of Streptomyces sp. as a Pancreatic Lipase Inhibitors Produced by Endophytic Streptomyces sp. AEBg12 Fitri, Lenni; Meryandini, Anja; Iswantini, Dyah; Lestari, Yulin
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8907

Abstract

Endophytic Streptomyces sp. AEBg12 isolated from Zingiber cassumunar (Bangle) is known to produce pancreatic lipase inhibitory compound. However, the characteristics of this active compound has not been reported yet. This study aimed to determine the characteristics of pancreatics inhibitory compound produced by Streptomyces sp. AEBg12 and to assess the role of endophytic actinobacteria in producing pancreatic lipase inhibitor using endophytic-free bangle tissue culture, wild bangle and compared with the activity of Streptomyces sp. AEBg12 endophytes. Supernatant of Streptomyces sp. AEBg12 was extracted using ethanol, ethyl acetate, and n-hexane solvents. Toxicity test was performed using larvae of shrimp Artemia salina. The results showed that the best solvent to obtain pancreatic lipase inhibitor compounds was ethanol. Phytochemical analysis showed that ethanolic extract of endophytic Streptomyces sp. AEBg12 contained flavonoids. IC50 value of ethanol extract was 180.83 g/ml. The result of TLC showed that ethanolic extract of Streptomyces AEBg12 had a blue luminescence band indicated that there were either flavone, flavanones, flavonols or isoflavones. Inhibitory activity of Streptomyces sp. AEBg12 was higher than wild bangle and bangle tissue culture. The information from this study can be be used as a basic data for further characterization of the active compound, which might be developed as an antiobesity agent through its pancreatic lipase inhibitory activity.
Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial Agent Fitriandini, Risa; Budiarti, Sri; Lestari, Yulin
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10323

Abstract

Rhododendron has long been known to treat various diseases including diarrhea, but diversity and potency of its endophytic actinobacteria has not been studied. The objectives of this research were to explore the existence of endophytic actinobacteria from Rododendron spp. and assesed their antibacterial activity, as an effort to control the growth of bacterial pathogen resistant to some antibiotics. The endophytes were isolated from Rhododendron spp. using HV medium, and purified in ISP2 medium. The antibacterial activity was assayed against Enteropathogenic Escerichia coli (EPEC) K1.1 resistant to ampicillin and Bacillus pumilus. The Minimum Inhibitory Concentration (MIC) value, macroscopic and microscopic were examined. Twenty three of endophytic actinobacteria were successfully isolated from 7 Rhododendron species. Two of them, i.e. RJkb1 and RJkb3 isolates, had high antibacterial activity, with 17.2 mm and 14.5 mm inhibition zone against EPEC K1-1, respectively; and 12.4 mm and 16.1 mm inhibition zone against B. pumilus, respectively. The highest antibacterial activity for both RJkb1 and RJkb3 isolates was achieved at day 15, at 28 oC. At 250 g/mL to 1750 g/mL either RJkb1 or RJkb3 supernatant showed no activity against EPEC K1-1. The MIC value against B. pumilus was at 1250 g/mL for both tested isolates. Under an electron microscope observation, cell morphology of the treated B. pumilus showed elongated cells and viewer in cell number, compared with the untreated one. From this work, the existence of endophytic actinobacteria from Rhododendron spp. and their antibacterial activity contribute to the understanding of their diversity and potency as antibacterial agent.
Exploration of Potential Actinomycetes from CIFOR Forest Origin as Antimicrobial, Antifungus, and Producing Extracellular Xylanase Sipriyadi, Sipriyadi; Lestari, Yulin; Wahyudi, Aris Tri; Meryandini, Anja; Suhartono, Maggy Thenawidjaja
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5052

Abstract

This study aimed to isolate and explore the actinomycetes of CIFOR forest origin as an antimicrobial and antifungal agent, to produce an extracellular xylanase, and to identify isolates based on 16S rRNA gene sequences. Actinomycetes were isolated using Humic-acid Vitamin-B agar (HV) media. Actinomycetes colonies that grow on the medium HV was subsequently purified by growing them on yeast malt agar (YMA) media), then an antagonistic test of selected bacteria against Bacillus sp., Escherichia coli, Fusarium oxysporum, and Sclerotium sp was performed. Xylanase activity test was detected by observing a clear zone, followed by identification. Total of 35 isolates of actinomycetes isolated based on their colony morphology characteristics and diverse types of spore chains showed Streptomyces spp. of isolates CFR-06, CFR-15, CFR-17, CFR-18, and CFR-19 were able to inhibit the growth of Bacillus sp.. The highest inhibition zone has a diameter of 10.1 mm (isolate CFR-17). Isolates CFR-01 and CFR-15 were able to inhibit the growth of E. coli with the highest inhibition zone diameter of 5.1 mm (isolate CFR-15). Isolates CFR-29 and CFR-12 were able to inhibit the growth of F. oxysporum while isolate CFR-35 were able to inhibit the growth of Sclerotium sp.. Xylanase activity test showed that isolates CFR-12, CFR-20, CFR-22, CFR-24, CFR-25, CFR-30, CFR-33, CFR-34 have an ability to produce extracellular xylanase enzyme. Actinomycetes isolate (Xyl_22) as a potential xylanase enzyme producer was closely related with Streptomyces drozdowicii by the maximum similarity of 99%.How to CiteSipriyadi, S., Lestari, Y., Wahyudi, A., Meryandini, A., & Suhartono, M. T. (2016). Exploration Potential CIFOR Forest actinomycetes origin as Antimicrobial, Anti Fungus and Producing Enzymes Extracellular Xylanase. Biosaintifika: Journal of Biology & Biology Education, 8(1), 94-102.
EFFECT OF POVIDONE IODINE TREATMENT ON BACTERIAL COMMUNITY ASSOCIATED WITH WHITE SHRIMP (LITOPENAEUS VANNAMEI) LARVAE Pangastuti, Artini; Suwanto, Antonius; Lestari, Yulin; Suhartono, Maggy T.
Marine Research in Indonesia Vol 34, No 2 (2009)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/mri.v34i2.471

Abstract

The efficacy of povidone iodine as a surface disinfectant was assessed in white shrimp (Litopenaeus vannamei) eggs and larvae. Eggs and nauplii were exposed to povidone iodine 20 ppm for 20 seconds. Culture-based method and Terminal Restriction Fragment Length Polymorphism (T-RFLP) were used to monitor the total number of bacteria and diversity of the bacterial community associated with shrimp eggs and larva at each developmental stage. Povidone iodine reduced the total culturable bacteria, especially Vibrio, on eggs and nauplii, as well as the total bacteria in the whole community as estimated by T-RFLP results. Povidone iodine also reduced the diversity of bacterial community and altered the evenness of phylotypes distribution suggesting that the use of povidone iodine as a surface disinfectant in shrimp aquaculture should be reconsidered.
Diversty of Actinomycetes from Soil Samples Collected from Lombok Island, Indonesia Lisdiyanti, Puspita; Tamura, Tomohiko; Ratnakomala, Shanti; Ridwan, Roni; Kartina, Gina; Lestari, Yulin; Katsuhiko, Ando; Widyastuti, Yantyati
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.783 KB) | DOI: 10.1234/52

Abstract

A total of 137 strains of Actinomycetes were isolated from 14 soil samples collected in 3 part of Lombok Island, Indonesia  using  SDS-YE (SY) and Rehydration and Centrifugation  (RC)  isolation methods  and Humic Acic Vitamin (HV) agar as isolation media. All the isolates were identified by morphological characteristic and by analysis of 16S rRNA gene sequence. On the basis of their morphology and 16S rRNA gene sequence, 67% of isolates were belonged  to the Streptomyces Group and 33% of isolates were belonged to the Rare-Actinomycetes (Non-Streptomyces) Group. Nine families and 15 genera were found from the samples. It is indicated the richness of actinomycetes in these area. The genus  Streptomyces  is the most abundant in all soil samples, occupying 67% of all isolates by using SY method; while the genusActinoplanes  is mainly found by using RC as isolation method. Two strains belong to the genus Virgisporangium and Catenulispora that showed taxonomic interest need further study for describing as ne species.   Keywords: Actinomycetes, Streptomyces Group, Non-Streptomyces Group, Diversity
Antioxidant and Antiglycation Activity of Rhizosphere and Endophytic Actinobacteria of Xylocarpus granatum Ariansyah, Alifian; Batubara, Irmanida; Lestari, Yulin; Egra, Saat
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.478 KB) | DOI: 10.15294/biosaintifika.v11i2.20018

Abstract

Utilization of endophytic bacteria such as actinobacteria is one of the alternatives to obtain the bioactive compounds similar to the host plant. This study aimed to identify compounds produced by rhizosphere and endophytic actinobacteria isolated from Xylocarpus granatum for their antioxidant and antiglycation activity. Actinobacterial culture supernatant was extracted using ethyl acetate with a ratio of 1:1 (v/v). Antioxidant activity was performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Whole TLC-bioautography and phytochemical tests were used to predict the group of active compounds. Antiglycation activity was identified based on the ability to inhibit the formation of advanced glycation end products (AGEs). Fifteen actinobacterial isolates had been isolated from rhizosphere (ten isolates), fruit (two isolates), and leaf (two isolates) of X. granatum. All isolates had been tested for their antioxidant and antiglycation activity. Isolate XR2 had the highest antioxidant activity with IC50 value of 1719.26 mg/L. Meanwhile, the highest antiglycation activity was obtained from isolate XR8 with IC50 value of 327.62 mg/L. This study informs that actinobacteria also live in the fruit, leaves and rhizosphere of X. granatum. The existence of rhizosphere and endophytic actinobacteria from X. granatum and their antioxidant and antiglycation activities contributes to the understanding of their diversity and potency as an antioxidant and antiglycation agent.
EFFECT OF POVIDONE IODINE TREATMENT ON BACTERIAL COMMUNITY ASSOCIATED WITH WHITE SHRIMP (LITOPENAEUS VANNAMEI) LARVAE Pangastuti, Artini; Suwanto, Antonius; Lestari, Yulin; Suhartono, Maggy T.
Marine Research in Indonesia Vol 34 No 2 (2009)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/mri.v34i2.471

Abstract

The efficacy of povidone iodine as a surface disinfectant was assessed in white shrimp (Litopenaeus vannamei) eggs and larvae. Eggs and nauplii were exposed to povidone iodine 20 ppm for 20 seconds. Culture-based method and Terminal Restriction Fragment Length Polymorphism (T-RFLP) were used to monitor the total number of bacteria and diversity of the bacterial community associated with shrimp eggs and larva at each developmental stage. Povidone iodine reduced the total culturable bacteria, especially Vibrio, on eggs and nauplii, as well as the total bacteria in the whole community as estimated by T-RFLP results. Povidone iodine also reduced the diversity of bacterial community and altered the evenness of phylotypes distribution suggesting that the use of povidone iodine as a surface disinfectant in shrimp aquaculture should be reconsidered.
APLIKASI MIKROORGANISME LOKAL (MOL) DIPERKAYA MIKROB BERGUNA PADA BUDIDAYA PADI SYSTEM OF RICE INTENSIFICATION (SRI) ORGANIK Batara, Lily Noviani; Anas, Iswandi; Santosa, Dwi Andreas; Lestari, Yulin
Jurnal Tanah dan Iklim (Indonesian Soil and Climate Journal) Vol 40, No 1 (2016)
Publisher : Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jti.v40n1.2016.71-78

Abstract

Abstrak. Mikroorganisme Lokal (MOL), suatu cairan bahan organik yang ditambahkan gula merah atau molase, berperan untuk meningkatkan pertumbuhan dan produksi serta mengatasi masalah hama dan penyakit tanaman padi pada System of Rice Intensification (SRI) organik. Sifat MOL sangat beragam dan sering tidak mengandung mikrob berguna. Tujuan penelitian ini adalah untuk (i) mengevaluasi kualitas berbagai macam MOL, (ii) memperbaiki kualitas MOL dengan menambahkan mikrob berguna, serta (iii) menguji pengaruh MOL yang diperbaiki kualitasnya terhadap pertumbuhan dan produksi tanaman padi dengan metode SRI organik. Pengujian kualitas dan perbaikan kualitas MOL dilakukan di Laboratorium dan di lapang di Desa Ciasihan, Kecamatan Pamijahan, Kabupaten Bogor, Jawa Barat. Mikrob berguna yang digunakan untuk memperbaiki kualitas MOL yaitu Azotobacter sp., Azospirillum sp., bakteri pelarut fosfat, dan Trichoderma harzianum. Hasil penelitian menunjukkan bahwa kualitas MOL yang diproduksi oleh petani sangat beragam sifat fisik, kimia dan biologinya. Kandungan unsur hara N paling tinggi terdapat pada MOL krokot 0,15%, unsur hara P pada MOL krokot dan nasi 0,06%, sementara unsur hara K pada MOL rebung 0,63%. Pembuatan MOL secara kuantitatif dan penambahan mikrob berguna ke dalam MOL mampu meningkatkan kualitas MOL yang dapat dilihat dari peningkatan pertumbuhan dan hasil tanaman padi. Jumlah anakan MOL rebung diperkaya mikrob berguna 42 batang rumpun-1 lebih tinggi bila dibandingkan dengan perlakuan MOL rebung 39 batang rumpun-1. Jumlah gabah dengan MOL rebung diperkaya mikrob berguna rata-rata 148,5 gabah malai-1, lebih tinggi dibandingkan dengan MOL rebung 142,5 gabah malai-1. Berat gabah kering panen MOL rebung diperkaya mikrob 10,7 t ha-1, lebih tinggi bila dibandingkan dengan MOL rebung setinggi 9,3 t ha-1.Abstract. Indigenous Microbes (IMO), an organic liquid product enriched with palm sugar or molasses, can be used to improve the growth and yield as well as to protect plants from pest and diseases in the Organic System of Rice Intensification (SRI). IMO characteristics vary and some do not contain beneficial microbes. This research was aimed to (i) evaluate the quality of IMO, (ii) to improve IMO quality by enriching with beneficial microbes and (iii) to evaluate the effects of enriched IMO on rice growth and yield under the Organic SRI. Chemical, physical and biological properties of IMO were evaluated at the Laboratory and the field trial was performed at Ciasihan village, Pamijahan District, Bogor, West Java. Benefical microbes used to improve the quality of IMO were Azotobacter sp., Azospirillum sp., phosphate solubilizing bacteria and Trichoderma harzianum. The results of this study showed that the physical, chemical and biological properties of IMO produced by farmers varied. Purslane IMO was highest in N content (0.15%), P content in purslane and rice IMO was 0.06% while K content in bamboo shoot IMO was 0.63%. Quantitative preparation of IMO and enrichment with beneficial microbes is necessary to improve its quality as can be observed from the improvement of rice growth and yield. Numbers of tillers of bamboo shoots of IMO enriched with beneficial microbes was 42 tillers hill and was higher compared to the treatment of bamboo shoots IMO without microbe enrichment which was 39 tillers hill-1. The number of rice grain under bamboo shoot IMO enriched beneficial microbes was 148.5 grains panicle-1 which was higher than that of the bamboo shoot IMO without enriched beneficial microbes of 142.5 grains panicle-1. Grain yield under bamboo shoot IMO enriched beneficial microbes was also higher, i.e. 10.7 t ha-1, which was higher compared to bamboo shoots IMO without enriched beneficial microbes of 9.3 t ha-1.
Pancreatic Lipase Inhibitory Activity of Endophytic Actinobacteria from Rhododendron spp. Winanda, Witri; Batubara, Irmanida; Lestari, Yulin
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.29981

Abstract

Antiobesity medication is available as therapeutic compounds that can reduce fat digestion by the inhibition of pancreatic lipase. Actinobacteria have the potency as source of bioactive compounds with various biological function including as pancreatic lipase inhibitor.  However, the potency of endophytic actinobacteria from Rhododendron spp. as source of pancreatic lipase inhibitor producer has not been reported yet. The aim of this study was to examine the potential of pancreatic lipase inhibitory activity of 23 endophytic actinobacteria from Rhododendron spp.; to characterize their colony based on morphology and molecular analysis. Screening test of pancreatic lipase inhibitor was conducted using the supernatant of endophytic actinobacteria, lipase pancreatic porcine (L3126) and p-nitrophenyl butyrate. The supernatant of selected isolates was extracted using ethyl acetate. The result showed that various inhibitory activities ranging between 0.00 until 91.69%. There were 11 out of 23 isolates that have potential as pancreatic inhibitor. Amongst them, the extract of four selected isolates, i.e. RZP 1.3, RSSB 3.2, RSS 2.1, and RJB F3.2 demonstrated inhibitory percentage of more than 80%. The RJB F3.2 extract showed to have IC50 value by 431.48 µg mL-1 compared to control, i.e. Xenical (89.07 µg mL-1). Phytochemical analysis exhibited that the extract of the selected isolates contained alkaloid which may function as pancreatic lipase inhibitor. Based on the morphological character, the selected isolates have various morphological colonies and 16S rRNA gene sequence revealed the sequence homology to Streptomyces spp. The data clearly indicate that endophytic actinobacteria from Rhododendron spp. have potency as pancreatic lipase inhibitor producer and further studies could be explored for the development of antiobesity agent.
Characterization of Xylanase Streptomyces spp. SKK1-8 ANJA MERYANDINI; TRIO HENDARWIN; DEDEN SAPRUDIN; YULIN LESTARI
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.007 KB) | DOI: 10.4308/hjb.13.4.151

Abstract

Streptomyces spp. SKK1-8 producing xylanase was isolated from soil sample from Sukabumi West Java. The xylanase have an optimum condition at pH 6 and 50 0C. Addition of 5 mM Cu2+ decreased the xylanase activity up to about 77%, whereas not by other cations. The xylanase was stable at 3 0C for 48 hours, and the enzyme half lifetime was 1 hour 45 minute at 50 0C. This xylanase showed the highest activity on oatspelt xylan, and their molecular masses were estimated approximately 16.80, 15.21, and 13.86 kDa. HPLC analysis showed that xylosa and arabinosa were the main hydrolytic product of birchwood xylan. Key words: xilanase, Streptomyces spp., characterization, zymogram and SDS-PAGE, stability
Co-Authors Abdjad Asih Nawangsih Alifian Ariansyah Amor Tresna Karyawati, Amor Tresna Anas, Iswandi Ando Katsuhiko ANDO KATSUHIKO Anja Meryandini Anja Meryandini Anja Meryandini Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R ARI SUSILOWATI Ariansyah, Alifian Aris Tri Wahyudi ARTINI PANGASTUTI Arum Asiyanti Suhastyo, Arum Asiyanti Arum Asriyanti Suhastyo BAMBANG MARWOTO Batara, Lily Noviani Budi Wirawan Deden Saprudin DWI ANDREAS SANTOSA Dyah Iswantini Dyah Iswantini DYAH NOOR HIDAYATI Egra, Saat Endang Sukara EVI TRIANA Evi Triana Ferry Mutia Fitriandini, Risa GINA KARTINA Gina Kartina GINA KARTINA Hamim Hamim HIDEKI YAMAMURA Iman Rusmana Indah Puspita SARI Irmanida Batubara Irmanida Batubara Iswandi Anas Iswandi Anas KATSUHIKO ANDO Katsuhiko Ando Laksmita Prima SANTI Lenni Fitri Lenni Fitri Lia Aseptin Murdini Lilis Nuraida Maggy T. Suhartono Maggy Thenawidjaja Suhartono Mahyarudin, Mahyarudin Marina Ratte Mazidah Noer Inayah Min Rahminiwati MISA OTOGURO Misa Otoguro MONA PRIMANITA Niken Financia Gusmawati NITA ROSALINDA PRAYITNO Nita Rosalinda Prayitno Nunuk Widhyastuti NUNUK WIDHYASTUTI PUSPITA LISDIYANTI Puspita Lisdiyanti PUSPITA LISDIYANTI PUSPITA LISDIYANTI Puspita Lisdiyanti RASTI SARASWATI RASTI SARASWATI RATIH DEWI HASTUTI RATIH DEWI HASTUTI Ratih Dwi Hastuti ROHMATUSSOLIHAT ROHMATUSSOLIHAT RONI RIDWAN Roni Ridwan RONI RIDWAN Saat Egra SASMIATI FARACH DITA Shanti Ratnakomala SHANTI RATNAKOMALA Shanti Ratnakomala SHANTI RATNAKOMALA SHINJI MIYADOH Sipriyadi Sri Budiarti Poerwanto Suryo Wiyono SUSILOWATI1 SUSILOWATI1 TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Titi Candra Sunarti dan Michael (E-Jurnal Agro-Industri Indonesia) TOMOHIKO TAMURA TOMOHIKO TAMURA Tomohiko Tamura TRI RATNA SULISTIYANI TRIO HENDARWIN Umi Fatmawati Winanda, Witri Yantyati Widyastuti YANTYATI WIDYASTUTI Yantyati Widyastuti Yantyati Widyastuti