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Isolasi dan Aktivitas Antibakteri Aktinomisetes Asal Tanah Gambut Riau Linda, Tetty Marta; Roza, Rodesia Mustika; Yuliati, Rola; Wahyuliyanti, Wahyuliyanti
Jurnal Natur Indonesia Vol 10, No 1 (2007)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.074 KB) | DOI: 10.31258/jnat.10.1.18-23

Abstract

The aims of this study are to isolate actinomycetes from peat soil samples, to determine the ability of actinomycetes to inhibit the growth of Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus) and Gram negativebacteria (Escherichia coli and Pseudomonas sp.). A total of 14 actinomycetes strains were recovered from peat soil samples using pour plate method with Starch Casein Agar. The results showed that 11 isolates were active against B. subtilis, 8 isolates against S. aureus, 8 isolates were active against E. coli and 8 isolates againstPseudomonas sp. Two isolates (SM 1.3 and SM 1.6) were active against all bacterial targets.
Seleksi Aktinomisetes Penghasil Protease dari Tanah Gambut Desa Langkai, Siak, Riau LINDA, TETTY MARTA; MARTINA, ATRIA; FEBRIANTI, BERNADETA LENI; , HERLINDA; , TABRI
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Actinomycetes is well known as secondary metabolites producer and hence it has high pharmacological andcommercial interest. In this study, a total of 14 isolates of actinomycetes from peat soils in Langkai Village,Siak, Riau had been selected for their ability to produce protease. Extracellular protease from Actinomycetesisolates was characterized by incubating the isolate in Nutrient Agar media containing casein 1%. Screeningof actinomycetes isolates showed that five isolates had the ability to produce a clear zone with the value ofproteolytic index (IP) ranged from 5.73 to 11.15. Isolates L313 showed the highest IP value (11.15), as wellas the highest protease production (0.041 U/ml) in Nutrient Broth media containing casein 1% after 6 daysincubation at room temperature.Key words: actinomycetes, clear zone, peat soil, protease, proteolitic index
Uji Patogenisitas Fungi Entomopatogen Lokal Riau sebagai Agen Biokontrol Hama Rayap (Coptotermes curvignathus Holmgren) SINTAWATI, REGIANA; MARTINA, ATRIA; LINDA, TETTY MARTA
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Biopesticide is a technique that can be used to the control pest population in the environment. Thepurpose of this research was to isolate fungi from the soil using baiting and cadaver technique andpathogenicity test. Isolates which had high germinating conidia was tested their patogenicity againstCoptotermes curvignathus. A total of 12 isolates were baited from soil and cadaver samples at palm oilplantation. The result showed eight isolates had high germinating conidia categories (˃ 80%). Thepathogenicity test of four isolates showed 100% mortality for RB.r01 and BK.r01; and KB.r01 andNK.r01were 72% and 67% respectively. Isolates capability against termite was not significantly different,but it was significantly different from control.Keywords : entomopathogenic fungi, pathogenicity, Coptotermes curvignathus, biocontrol
Biosynthesis of ZnO Micro-Nanoflower with Ananas comosus Peel Extract Maya Sari; Yolanda Rati; Tetty Marta Linda; Yanuar Hamzah; Ari Sulistyo Rini
Journal of Aceh Physics Society Volume 10, Number 4, October 2021
Publisher : PSI-Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jacps.v10i4.18951

Abstract

 Abstrak. Dalam rangka mengurangi pemakaian bahan kimia berbahaya, kini telah mulai dikembangkan penggunaan bahan alami pada proses pembentukan nanopartikel. Pada penelitian ini, biosintesis nanopartikel ZnO dilakukan menggunakan ekstrak kulit Ananas comosus sebagai agen capping sekaligus agen pereduksi. Seng nitrat digunakan sebagai prekursor dari ion seng, sedangkan kulit nanas dibuat menjadi ekstrak untuk dimanfaatkan kandungan antioksidannya. Sampel ZnO dipelajari sifat fisisnya dari hasil karakterisasi X-ray diffraction (XRD), scanning electron microscopy (SEM) dan spektroskopi UV-Vis. Berdasarkan pola XRD, nanopartikel ZnO memberikan fasa kristal heksagonal wurtzite dengan ukuran kristal 14 nm. Morfologi SEM masing-masing sampel didapatkan berbentuk bunga atau micro-nanoflower dengan ukuran diameter rata-rata 510 nm dan 560 nm untuk sampel 0,01 M dan 0,025 M. Hasil spektrum absorbansi UV-Vis menunjukkan peningkatan puncak penyerapan cahaya dengan penambahan konsentrasi seng nitrat. Berdasarkan informasi sifat fisis ini, sampel ZnO berpotensi diaplikasikan sebagai material fotokatalis.Abstract. In order to reduce the use of hazardous chemicals, the use of natural ingredients has now been developed in the process of forming nanoparticles. In this study, biosynthesis of ZnO nanoparticles was carried out using Ananas comosus peel extract as capping agent and reducing agent. Zinc nitrate was used as a precursor to zinc ion. The physical properties of ZnO samples were studied from the characterization result of scanning electron microscopy (SEM), UV-Vis spectroscopy, and X-ray diffraction (XRD). The SEM morphology of each different sample was obtained in the form of micro-nanoflower with an average diameter  of 510 nm and 560 nm for 0.01 M and 0.025 M samples, respectively. The UV-Vis absorbance spectrum results showed an increase in the light absorption peak as  zinc nitrate concentration increased. According to the XRD pattern, the ZnO nanoparticles possessed an hexagonal wurtzite crystal phase with a crystal size of 14 nm. Based on this information on physical properties, the ZnO sample has the potential to be applied as a photocatalyst material.
Seleksi Aktinomisetes Isolat Lokal Dari Tanah Gambut Riau Sebagai Antipatogen Pada Streptococcus pyogenes TETTY MARTA LINDA; LAMISSI NAPITUPULI; RODESIA MESTIKA ROZA
Prosiding Seminar Biologi Vol 2 No 1 (2016): Prosiding Seminar Nasional From Basic Science to Comprehensive Education
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v2i1.3392

Abstract

Dua puluh empat isolat aktinomisetes hasil isolasi dari tanah gambut Riau diseleksi menggunakan metode agar disk untuk dilihat aktivitas antipatogen pada Streptococcus pyogenes menggunakan medium Triptic Soy Agar (TSA). Hasil seleksi diperoleh lima isolat aktinomisetes (MH23, L313, L18, L223 dan MH11) menghasilkan zona bening disekitan bakteri uji. Isolat MH23 menghasilkan zona bening tertinggi yaitu 24 mm. Kelima isolat aktinomisetes berpotensi sebagai penghasil antibiotik yang bermanfaat bagi kesehatan.Kata kunci: Aktinomisetes, antibiotik, tanah gambut, Streptococcus pyogenes
Seleksi dan Akivitas Enzim Selulase Aktinomisetes Lokal Riau pada Media Lignoselulosa Ampas Tebu PESRITA, ANALIA; LINDA, TETTY MARTA; DEVI, SILVERA
Jurnal Riau Biologia Vol 2, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Ampas tebu merupakan limbah pertanian yang mengandung selulosa, hemiselulosa, dan lignin sehingga dapat digunakan sebagai media untuk menumbuhkan mikroorganisme yang mampu menghasilkan enzim selulase, xylanase dan ligninase. Tujuan dari penelitian ini adalah untuk melihat kemampuan isolat aktinomisetes lokal dalam menghidrolisis substrat pada medium CMC (Carboxilmetil cellulose ) dan ampas tebu. Sebanyak 13 isolat aktinomisetes lokal diseleksi dan diuji aktivitas enzim selulase yang dihasilkannya pada medium CMC dan medium ampas tebu. Seleksi dilakukan secara kualitatif berdasarkan rasio zona bening yang terbentuk, sedangkan isolat dengan rasio tertinggi akan diuji aktivitas selulase yang dihasilkannya secara kuantitatif menggunakan dengan metode DNS (Dinitro Salicylic Acid). Hasil yang diperoleh menunjukkan semua isolat mampu memperlihatkan selulolitik hidrolitik selulase baik pada medium CMC dan medium ampas tebu. Rasio zona bening pada medium ampas tebu lebih tinggi dari medium CMC yaitu sebesar 40,72 ± 0,95 yang diperlihatkan oleh isolat L223. Uji kuantitatif dari aktivitas selulase isolat L223 sebesar 35,30 x10-3 U/ml.
Seleksi Isolat Aktinomisetes Asal Tanah Gambut Desa Rimbo Panjang Kabupaten Kampar Dalam Menghasilkan Hormon IAA (Indole Acetic Acid) MAWARTI, INDAH; FIBRIARTI, BERNADETA LENI; ZUL, DELITA; ROZA, RODESIA MUSTIKA; MARTINA, ATRIA; LINDA, TETTY MARTA
Jurnal Riau Biologia Vol 2, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Hormon IAA (Indole Acetic Acid) termasuk fitohormon golongan auksin yang berperan sebagai zat pemacu pertumbuhan tanaman. Selain tumbuhan, mikroba juga diketahui mampu menghasilkan IAA. Penelitian ini bertujuan untuk menyeleksi kemampuan koleksi isolat aktinomisetes Laboratorium Mikrobiologi FMIPA UR dalam menghasilkan IAA. Uji produksi IAA oleh aktinomisetes dilakukan dengan penambahan reagen Salkowski menggunakan metode kolorimetri dalam medium SCB (Starch Casein Broth) yang diperkaya dengan triptofan sebagai prekursor dan tanpa triptofan. Hasil penelitan ini diperoleh sebanyak 50 dari 85 isolat aktinomisetes yang memiliki kemampuan dalam menghasilkan hormon IAA. Produksi IAA tertinggi dihasilkan oleh RB5S78 sebesar 35,97 ppm dan konsentrasi terendah dihasilkan oleh RB4S67 sebesar 0,24 ppm dalam medium SCB yang diperkaya triptofan. Pada medium tanpa triptofan hasil tertinggi diperoleh isolat aktinomisetes RB5S78 dengan konsentrasi sebesar 15,28 ppm dan konsentrasi terendah dihasilkan oleh RB4S65 sebesar 0,16 ppm. Isolat aktinomisetes yang menghasilkan IAA dengan kriteria tinggi yang diperoleh termasuk dalam genus Streptomyces dan Rhodococcus.
KEMAMPUAN ISOLAT TUNGGAL DAN KONSORSIUM AKTINOMISETES LOKAL RIAU DALAM MENDEGRADASI HIDROKARBON MINYAK BUMI Novalita Dwi Fanny; Tetty Marta Linda; Atria Martina
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 2 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.94 KB) | DOI: 10.22437/bs.v4i2.5691

Abstract

Pollution of crude oil on environmental can have an effect of nutrient uptake on plants and balance of ecosystems. Biodegradation. is one way to reduce petroleum pollution . The purpose of this research are determine the potential growth of actinomycetes local Riau (L11 and L121) in crude oil hydrocarbons at concentrations of 0%, 2%, 5% and to know their ability to degrade crude oil hydrocarbons at concentrations of 5%. This research observed the growth of each actinomycetes local Riau and the percentage of crude oil degradation by a single isolate and a consortium with gravimetric method. The results showed that the growth of L11 and L121 total population was not significantly different at addition crude oil of 2% and 5% but significantly different wth 0% (without crude oil). Test degradation of crude oil using single isolate gave significantly different results for the consortium isolates. Percentage of degradation crude oil by L11 was 23.5%, L121 was 19.2% and consortium isolate (L11 + L121) was 31.9% with an incubation time of 10 days in a minimum medium salts. This actinomycetes local Riau can be developed as biodegradation agents for crude oil on soil and water.
STUDI PERTUMBUHAN DAN DEGRADASI FENOL OLEH KULTUR TUNGGAL AKTINOMISETES DARI TANAH GAMBUT Tiara Elsita Masni; Tetty Marta Linda; Bernadeta Leni Fibriarti
Al-Kauniyah: Jurnal Biologi Vol 13, No 1 (2020): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2866.46 KB) | DOI: 10.15408/kauniyah.v13i1.12854

Abstract

AbstrakFenol adalah senyawa organik yang bersifat toksik dan larut dalam air, sehingga mudah menimbulkan pencemaran pada perairan dan menurunkan kualitas air. Penelitian ini bertujuan untuk melihat potensi tiga isolat aktinomisetes asal tanah gambut Riau dalam Minimal Salt Medium yang mengandung fenol pada konsentrasi 0 ppm, 200 ppm, 400 ppm, dan 600 ppm serta mengetahui kemampuan aktinomisetes dalam mendegradasi fenol pada konsentrasi 600 ppm menggunakan metode folin ciocalteau. Potensi pertumbuhan isolat L121, L18, L11 menunjukkan total populasi tidak berbeda nyata dengan penambahan 400 ppm dan 600 ppm fenol, tetapi berbeda nyata terhadap 0 ppm dan 200 ppm fenol. Potensi pertumbuhan tertinggi terdapat pada isolat L121 dan terendah pada isolat L11. Kemampuan degradasi  fenol oleh  masing-masing isolat adalah  L121 sebesar 570,80 ppm (95%),  L18 sebesar 218,85 ppm (36%)  dan L11 sebesar  97,21 ppm (16%)  dari konsentrasi fenol awal 600 ppm pada Minimal Salt Medium. Isolat aktinomisetes ini berpotensi dikembangkan untuk penanggulangan pencemaran di lingkungan.Abstract Phenol is an organic compound is toxic and easily soluble in water so easy to cause pollution in a waters such as water quality degradation. The aim of this research is to see the potential of three isolates of actinomycetes from Riau peat soil in Minimal Salt Medium containing phenol concentration 0 ppm, 200 ppm, 400 ppm and 600 ppm and to know the ability of actinomycetes in degradation of phenol at the concentration of 600 ppm using folin ciocalteu. The growth potential of L121, L18, L11 isolates showed the total population was not significantly different with the addition of 400 ppm and 600 ppm of phenol but significantly different from 0 ppm and 200 ppm of phenol. The highest growth potential was found in L121 isolate and lowest in L11 isolate. The degradation ability of phenols by each isolate was L121 570.80 ppm (95%), L18 218.85 ppm (36%) and L11 was able to degrade phenol 97.21 ppm (16%) from the initial phenol concentration of 600 ppm at Minimum Salt Medium.These actinomycetes have the potential to be developed for the overcome of pollution in the environment.
Efficacy of Cellulolytic Bacteria Consortium for Composting Oil Palm Empty Bunches Containing Phytonutrients Aini, Diah Nurul; Linda, Tetty Marta
Jurnal Natur Indonesia Vol 18, No 1 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.212 KB) | DOI: 10.31258/jnat.18.1.12-19

Abstract

Oil palm empty fruit bunches (OPEFB) are themost solid palm oil waste. OPEFB has often been processed into compost with the addition of certain activators. It is expected that with the addition of a consortium bioactivator composting of OPEFB can be faster and the compost produced has good nutrient content. The study aims was to determine the ability of bioactivator bacteria of cellulolytic consortium in degrading TKKS of incubation laboratory scale for 30 days. A consortium of compost bioactivator used were Bacillus sp. S43, Bacillus cereus strains of IARI-MB-6, Bacillus cereus strains TS11, Alcaligenes faecalis strains ZJUTBX11, Bacillus sp. 13847, Stenotrophomonas sp. S169-III-5, Alcaligenes faecalis strains KH-48 and Bacillus cereus strain Y22 by a comparison of 1:1:1:1:1:1:1:1. The results showed that bioactivator consortium was able to degrade OPEFB which on P4 (OPEFB + chicken manure + consortium isolate) reduced organic C from 50.1 to 34.5, increased total nitrogen from 0.73 to 1.35 and reduced the C/N ratio from 37.11 to 25.56 and produced compost phytonutrients and not phytotoxicity.