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Potensi Ekstrak Kulit Jeruk Pacitan (Citrus sinensis) sebagai Stimulus Regenerasi Sel pada Luka Bakar Rattus norvegicus Samsudin, Rinza Rahmawati; Arimurti, Anindita Riesti Retno
Jurnal Labora Medika Vol 2, No 2 (2018): Jurnal Labora Medika
Publisher : Jurnal Labora Medika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Luka bakar adalah kerusakan jaringan yang terjadi pada permukaan kulit. Eritema adalah respons peradangan yang pertama kali muncul di daerah peradangan selama proses penyembuhan luka bakar. Semakin cepat derajat eritema menurun, semakin cepat proses penyembuhan untuk luka bakar. Ekstrak kulit jeruk pacitan mengandung senyawa aktif d-limonen, flavonoid, saponin, dan tanin yang dapat merangsang regenerasi sel dalam penyembuhan luka bakar. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstraksi kulit jeruk Pacitan untuk mengurangi eritema pada Rattus norvegicus penyembuhan luka bakar derajat II. Penelitian ini bersifat eksperimental. Penelitian ini menggunakan 25 tikus putih yang dibagi menjadi 5 kelompok perlakuan berbeda. Pada K0 sebagai kontrol, tikus putih yang mengalami luka bakar derajat II hanya diberikan aquadest, K1 diberi perlakuan luka bakar menggunakan normal saline, K2 diobati dengan ekstrak kulit jeruk Pacitan 40%, K3 diobati dengan luka bakar menggunakan ekstrak kulit jeruk Pacitan 60%. dan K4 diberikan perawatan luka bakar menggunakan ekstrak kulit jeruk Pacitan 80%. Perawatan dilakukan selama 7 hari. Data yang diperoleh dari penelitian kemudian dianalisis dengan Uji Parametrik One Way Analysis of Variance (ANOVA). Berdasarkan uji Homogenitas dengan mengolah data menggunakan uji Levene, diketahui bahwa nilai signifikansi 0,829> 0,05, sehingga dapat disimpulkan bahwa data bersifat homogen. Sedangkan untuk uji normalitas pengolahan data menggunakan uji Shapiro-Wilk dimana nilai P> 0,05 dapat disimpulkan bahwa data data berdistribusi normal. Hasil penelitian menunjukkan bahwa pemberian ekstrak kulit jeruk Pacitan selama 7 hari dapat mempercepat pengurangan eritema, secara signifikan dengan P <0,05.
Keanekaragaman Genetik Nyamuk Vektor Filariasis Culex quinquefasciatus Say, 1823 (Diptera: Culicidae) di Kota dan Kabupaten Pekalongan Dengan Metode PCR-RAPD Anindita Riesti Retno Arimurti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1087.745 KB) | DOI: 10.30651/jmlt.v1i2.1496

Abstract

Mosquito Culex quinquefasciatus is a vector of nematode worms, namely Wuchereria bancrofti which is the cause of filariasis in tropical and subtropical countries. Distributed of Cx. quinquefasciatus is widely in Indonesia with differences the geographical, resulting in the adaptation to the environment and may  results in a high variation, both phenotypic (morphology) and genotypic (genetic) variation. This study aims was to determine the genetic diversity of mosquitoes Cx.quinquefasciatus as vector filariasis in Pekalongan City and Regent. Genetic characterization performed by PCR-RAPD using three primers, ie OPA-11, OPA-12, and OPA-15. Data were analyzed by using UPGMA algorithm and Simple Matching Coefficient and presented as dendrogram. The results showed a high genetic diversity with the polymorphisms up to 100%.  Keywords: Culex quinquefasciatus, vector, filariasis, PCR-RAPD 
Gambaran Parasit Cacing Nematoda Usus Dan Cestoda Pada Feses Sapi (Boss sp.) Di Peternakan Sumber Jaya Ternak, Kecamatan Tikung, Kabupaten Lamongan, Jawa Timur Anindita Riesti Retno Arimurti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 3, No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.751 KB) | DOI: 10.30651/jmlt.v3i1.4632

Abstract

Cows are important animals for Indonesian farmers who have high economic value. Cow breeding business has many benefits for human life, especially meat, milk, bones, skin, offal and feces (cow dung). Cow feces can be used as an organic fertilizer manufacture by cattle farmers. But it needs to be considered in making organic fertilizer derived from cows because it contains many diseases and parasites. If the cow's feces are not treated properly, it will cause environmental pollution to the local residents. Cow infected with parasites can experience a decrease in body weight, decreased endurance, impaired growth and death. To find out intestinal helminth parasitic infections, one of them is by identifying worms in cow feces. The majority of the population of Tikung Subdistrict, Lamongan Regency, earn their living as farmers and ranchers. The purpose of this study was to identify intestinal Nematode worms in cow feces in Tikung Subdistrict, Lamongan Regency. The method used in this study was Nacl Saturated to determine the morphology and forms of parasites. The results showed that cattle feces samples taken from the Tikung Subdistrict of Lamongan District contained the Nematoda parasite, which was found the presence of Hookworm eggs in 3 samples with a percentage of 6% and found 1 positive sample (+) containing Taenia saginata eggs, with a percentage of 2% of the total 50 samples in Sumber Jaya Livestock Farm in cattle on Tikung Subdistrict, Lamongan Regency is still relatively low. Keywords: Cow Feces (Bos sp.), Intestinal Nematodes, Cestodes
Efektivitas Minyak Atsiri Serai Wangi (Combypogon nardus) Sebagai Insektisida Alami Untuk Kecoa Amerika (Periplaneta americana) Anindita Riesti Retno Arimurti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.116 KB) | DOI: 10.30651/jmlt.v1i1.1008

Abstract

  ABSTRAK Cockroaches are one of the most common types of insects around our neighborhood, and it is one of the most dangerous insects because some species of cockroaches are known to transmit disease in humans such as tuberculosis, typhoid, asthma, cholera, and hepatitis. Eradication of cockroaches can be done physically and chemically, the use of chemical-based insecticides can cause respiratory problems. In an insecticide drug containing natural ingredients contain essential oils, the content of essential oil compounds serve as an anti-insect drug on citronella. This study aimed to determine the effect of essential oils of citronella fragrance to cockroaches. This type of research was experimental. The population in this study were cockroaches (Periplaneta americana) obtained from the houses of villagers of Tanjung, Sampang, Madura. The total sample was 32 adult stage cockroaches. Data analysis was using free T test with error rate of  5% or 0,05.The results showed that cockroaches stopped moving with an average of 15.75. Based on T test results showed the significant results of 0.00 which was smaller than 0.05 then H0 was rejected Ha was accepted. It can be concluded that the essential oils of citronella fragrance have an effect on the death of cockroaches. Keywords: Cockroach, Essential Oil Of Citronella Fragrance (Cymbopogon nardus)
Test the effectiveness of Breadfruit (Artocarpus altilis) leaf extract on the growth of Staphylococcus aureus Fitrotin Azizah; Anindita Riesti Retno Arimurti; Dita Artanti; Yeti Eka Sispita Sari
Journal of Natural Sciences and Mathematics Research Vol 6, No 1 (2020): June
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/jnsmr.2020.6.1.11151

Abstract

Infectious diseases caused by Staphylococcus aureus are a health problem in Indonesia. Antibiotics can be used as the treatment, but currently Staphylococcus aureus is resistant to antibiotics. Therefore, it is necessary to find alternative medicine. One of the plants that has characteristics to be used as herbal medicine is Breadfruit leaves. The purpose of this study was to determine the effect of concentration on breadfruit leaf juice which can inhibit the growth of Staphylococcus aureus bacteria. This type of research is experimental. The study population was pure Staphylococcus aureus bacteria on NAS (Nutrient Agar Slant) media. The sample in this study was pure Staphylococcus aureus transferred from pure culture media (NAS), while the number of repetitions of the sample was 5 times the treatment, in this study were P1 (100%), P2 (75%), P3 (50%), P4 (25%), P5(0%). Data on the growth of Staphylococcus aureus bacteria was obtained by indirect observation, namely through laboratory tests and data collection techniques using the ANOVA test with an error rate (0.05) and followed by the Tukkey HSD test through the SPSS16.0 program. Based on the results of the antibacterial test of breadfruit leaf juice (Artocarpus altilis) in the ANOVA test, the probability number was 0.000 and there was an effect on the growth of Staphylococcus aureus bacteria. Followed by the Tukkey HSD test, it was obtained at a concentration of 25% which is the minimum inhibitory power, the effective concentration to inhibit the growth of Staphylococcus aureus at a concentration of 75%. From the data above, it can be concluded that there is an effect of breadfruit leaf juice (Artocarpus altilis) on the growth of Staphylococcus aureus.©2020 JNSMR UIN Walisongo. All rights reserved. 
Interpretation of external quality assurance results on liver function test Rahma Widyastuti; Nur Vita Purwaningsih; Rinza Rahmawati Samsudin; Anindita Riesti Retno Arimurti
Journal of Natural Sciences and Mathematics Research Vol 6, No 2 (2020): December
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/jnsmr.2020.6.2.11221

Abstract

The quality of laboratory examination results must always be guaranteed in the sense that it can provide a level of accuracy and precision that can be accounted for. Therefore, a study of External Quality Consolidation (PME) was conducted. The purpose of this study was to describe the results of External Quality Monitoring in the field of clinical chemistry parameters of Serum Glutamic Oxalocetic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT) at the Public Health Center Laboratory in the Surabaya area. The population in this study was a health center in the area of the Surabaya City Health Office with a sample of 15 health centers that had examinations in the field of clinical chemistry. The sample used in this study was normal level control serum which was measured on the parameters of Serum Glutamic Oxalocetic Transaminase (SGOT) and Serum Glutamic Pyruvic Oxalocetic Transaminase (SGPT). The results of data analysis showed that the percentage of PME levels of SGOT with good VIS criteria was 73.33%, sufficient criteria was 0%, less criteria was 6.67%, poor criteria was 20%. While the SGPT parameters good criteria are 53.33%, sufficient criteria are 33.33%, less criteria are 13.33%, poor criteria are 0%.©2020 JNSMR UIN Walisongo. All rights reserved.
Potensi Ekstrak Kulit Jeruk Pacitan (Citrus sinensis) sebagai Stimulus Regenerasi Sel pada Luka Bakar Rattus norvegicus Rinza Rahmawati Samsudin; Anindita Riesti Retno Arimurti
Jurnal Labora Medika Vol 2, No 2 (2018): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (462.719 KB) | DOI: 10.26714/jlabmed.2.2.2018.19-23

Abstract

Luka bakar adalah kerusakan jaringan yang terjadi pada permukaan kulit. Eritema adalah respons peradangan yang pertama kali muncul di daerah peradangan selama proses penyembuhan luka bakar. Semakin cepat derajat eritema menurun, semakin cepat proses penyembuhan untuk luka bakar. Ekstrak kulit jeruk pacitan mengandung senyawa aktif d-limonen, flavonoid, saponin, dan tanin yang dapat merangsang regenerasi sel dalam penyembuhan luka bakar. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstraksi kulit jeruk Pacitan untuk mengurangi eritema pada Rattus norvegicus penyembuhan luka bakar derajat II. Penelitian ini bersifat eksperimental. Penelitian ini menggunakan 25 tikus putih yang dibagi menjadi 5 kelompok perlakuan berbeda. Pada K0 sebagai kontrol, tikus putih yang mengalami luka bakar derajat II hanya diberikan aquadest, K1 diberi perlakuan luka bakar menggunakan normal saline, K2 diobati dengan ekstrak kulit jeruk Pacitan 40%, K3 diobati dengan luka bakar menggunakan ekstrak kulit jeruk Pacitan 60%. dan K4 diberikan perawatan luka bakar menggunakan ekstrak kulit jeruk Pacitan 80%. Perawatan dilakukan selama 7 hari. Data yang diperoleh dari penelitian kemudian dianalisis dengan Uji Parametrik One Way Analysis of Variance (ANOVA). Berdasarkan uji Homogenitas dengan mengolah data menggunakan uji Levene, diketahui bahwa nilai signifikansi 0,829> 0,05, sehingga dapat disimpulkan bahwa data bersifat homogen. Sedangkan untuk uji normalitas pengolahan data menggunakan uji Shapiro-Wilk dimana nilai P> 0,05 dapat disimpulkan bahwa data data berdistribusi normal. Hasil penelitian menunjukkan bahwa pemberian ekstrak kulit jeruk Pacitan selama 7 hari dapat mempercepat pengurangan eritema, secara signifikan dengan P <0,05.
Pemeriksaan Cemaran Kapang Pada Udara Dan Pakaian Santri Di Salah Satu Pondok Pesantren Surabaya Timur Anindita Riesti Retno Arimurti; Fitrotin Azizah
Al-Hayat: Journal of Biology and Applied Biology Vol 4, No 1 (2021)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v4i1.7062

Abstract

Dormitory is a place for student who built in various small scales to a very large scale. Of the many dormitory residents with bad space such as lack of ventilation and incoming sunlight, the large number of scattered students' clothes causes the room to become damp. The presence of air mold is caused by the lack of ventilation as well as the humidity of the room environment in the dormitory which can cause several diseases, one of which is ARI. The purpose of this study was to determine whether or not there is mold contamination on air in the room and clothes of the students at one of the Islamic boarding schools in East Surabaya. This type of research is descriptive. The sample used is air in several dormitory rooms at one of the Islamic boarding schools in East Surabaya. This research method uses the isolation technique of Sabauraud Dextose Agar (SDA) media which is partially opened and then placed in several rooms in this Islamic boarding school. Second, 30 samples of clothes were taken randomly from students from different disciplines and then scratched on SDA media. In the results of macroscopic observations, of the 30 sampled rooms. Microscopic colonies of cotton taken from the media showed positive results for hyphae, mycellium, spores, and conidiospores. The results obtained were positive there were Aspergillus sp.  with the percentage was 100% while the results of the students' clothing swabs, from 30 clothing samples found 80% positive for mold contamination.
The potency of bacteria isolated from the hydroponic rockwool of field mustard (Brassica rapa L.) for nitrogen fixation and indole acetic acid (IAA) production Anindita Riesti Retno Arimurti; Vella Rohmayani; Nurhidayatullah Romadhon; Tara Puri Ducha Rahmani; Lee James Watson; Kenari Sri Wahyuni; Nova Ulumiya
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 1 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i1.28451

Abstract

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EDUKASI DAN PELAYANAN KARYAWAN DI UNIVERSITAS MUHAMMADIYAH SURABAYA MELALUI KONSUMSI BAKTERI ASAM LAKTAT UNTUK PENINGKATAN SISTEM IMUN DI COVID-19 Dita Artanti; Nur Vita Purwaningsih; Yeti Eka Sispita Sari; Fitrotin Azizah; Rinza Rahmawati Samsudin; Vella Rohmayani; Anindita Riesti Retno Arimurti; Mulya Fitrah Juniawan
Humanism : Jurnal Pengabdian Masyarakat Vol 3, No 2 (2022): Agustus
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/hm.v3i2.14354

Abstract

Pada tahun 2020 seluruh dunia sedang mengalami kondisi pandemik akibat infeksi virus. Virus SARS-CoV-2 yang dapat menyebabkan penyakit COVID-19. Kondisi ini mengakibatkan “Panic Buying”, karena semua orang mencoba untuk menghindari penyebaran dan penularan penyakit COVID-19 dengan melakukan berbagai cara, salah satunya dengan mengambil tambahan suplemen seperti vitamin C dan suplemen probiotik. Probiotik merupakan kelompok bakteri asam laktat, jika dikonsumsi dalam jumlah yang cukup dapat memberikan manfaat kesehatan bagi tubuh. Bakteri asam laktat memiliki fungsi sebagai imunomodulator atau meningkatkan sistem kekebalan tubuh sehingga dapat mencegah infeksi penyakit pada hewan dan manusia. Kegiatan edukasi dan pelayanan dilakukan melalui poster dan penyampaian langsung dengan metode door to door. Sehingga karyawan di Universitas Muhammadiyah Surabaya lebih mudah memahami terkait konsumsi bakteri asam laktat untuk peningkatan sistem imun di COVID-19. Para karyawan sangat antusias dengan kegiatan edukasi yang dilakukan, mereka juga menjadi lebih disiplis untuk menghindari penularan dan pencegahan penyakit COVID-19.Kata Kunci: edukasi dan pelayanan karyawan, COVID-19, Bakteri Asam Laktat, dan sistem imun.