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Pertumbuhan Bibit Tanaman Manggis (Garcinia mangostana L.) Setelah Inokulasi dengan Berbagai Galur Agrobacterium rhizogenes1 , Lizawati; Roedhy Poerwanto; , Sobir; Iman Rusmana; Tri Muji Ermayanti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 2 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.098 KB) | DOI: 10.24831/jai.v35i2.1321

Abstract

Growth of mangosteen essentially depends on its root system.  Therefore, it needs technology to obtain stringer mangosteen root system.  The use of Agrobacterium rhizogenes bacterium is an alternative.  The objectives of this experiment were : 1) to find the effective strain of A. rhizogenes bacterium for inoculation of mangosteen seedling root, 2) to find the best inoculation method for inducing mangosteen seedling root.  The materials used in this experiment were ; mangosteen fruit and A. rhizogenes collection from Puslit Biotechnology LIPI Cibinong-Bogor.  The experiment was arranged in completely randomized design with two factorial treatments.  The first factor : 11 strains A. rhizogenes (ATCC-15834, ATCC-8196, R-1000, 07-20001, A4, A4-J, 509, 510, 511, MAFF 01-1724, and control), the second factor : 2 inoculation methods (cutting and dipping).  The results showed that A. rhizogenes  of ATCC-15834, 509, 07-20001, A4, and R-1000 increased stem diameter, plant height, leaf number, lateral and tertiary root number, better than ATCC-8196, MAFF 01-1724, 510, 511, A4-J, and control.  Cutting root method of inoculation resulted in higher live plant percentage compared to dipping root method.   Key words :  Agrobacterium rhizogenes, Garcinia mangostana, inoculation
Proliferasi Kalus dari Eksplan Hipokotil dan Kotiledon Tanaman Jarak Pagar (Jatropha curcas L.) pada Pemberian 2,4-D Zulkarnain, Zulkarnain; Lizawati, Lizawati
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.286 KB) | DOI: 10.31258/jnat.14.1.19-25

Abstract

The aim of this study was to develop an efficient method for the induction of embryogenic callus formation for in vitro propagation ofjatropha. Plant materials used were 30-days old in vitro seedlings, cut into hypocotyl and cotyledon (lower, middle and upper) sections.Medium used was MS composition supplemented with vitamins, 3% sucrose, 0.7% agar at pH 5.8 ± 1, and 2,4-D (0, 1, 2, 3, 4 dan5 mg l-1). Cultures were kept at temperature of 25 ± 1 0C with 50 μmol m-2 s-1 light intensity and 16-h photoperiod. The results indicated thatthe rate of callus formation depended on the source of explant, the application of 2,4-D, and the interaction of both. The fastest callusproliferation (2.33 days following initiation) was obtained on cotyledon explants cultured on medium without 2,4-D. The explant sourcesand 2,4-D concentrations also showed significant effect on the percentage of explant forming callus. The most callus formation (88.33%)was obtained on middle cotyledon cultured on 3 mg l-1 2,4-D, whereas the fewest (6.84%) was found on upper cotyledon cultured on mediumwithout 2,4-D. The colour of callus was dominated by white, light yellow, cream and brown with mostly compact structure, particularly onhypocotyl cultured on medium without 2,4-D. The texture of callus formed on hypocotyl treated with up to 4 mg l -1 2,4-D was dominatedby coarse appearance. In contrast, majority of callus proliferated on hypocotyl treated with 5 mg l -1 2,4-D or cotyledon treated with orwithout 2,4-D produced callus with smooth texture %.
Callus Proliferation from Immature Leaf Explants of Durian (Durio zibethinus Murr. cv. Selat) with the Addition of Picloram and BAP Zulkarnain ,; , Lizawati
Jurnal Hortikultura Indonesia Vol. 4 No. 3 (2013): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.339 KB) | DOI: 10.29244/jhi.4.3.107-114

Abstract

ABSTRACTThis study was aimed to obtain an appropriate medium composition with various combinations of Picloram + BAP for the proliferation of embryogenic callus from immature leaf explants of durian. The experiment was carried out at the Plant Biotechnology Laboratory,  Faculty of Agriculture ,  the University of Jambi from January through to November 2012. Five levels of Picloram (1.0, 2.0, 3.0, 4.0, 5.0 mg  L-1) in combination with three levels of BAP (0, 0.5, 1.5 mg  L-1) were tested. Therefore, there were 15 treatment combinations with 4 replicates resulting in  60 experimental unit. Each unit  consisted of 4 culture flasks containing one immature leaf explant. Cultures were kept in culture room with 16 h photoperiod and 1000 lux light intensity. The results showed that: 1) callus proliferation on immature leaf explants of durian cv. Selat was dependent upon the level of Picloram + BAP added to culture medium, 2) the addition of 3.0 -  5.0 mg L-1Picloram without BAP was found to be effective in promoting callus proliferation on the majority of cultured explants, 3) all regenerated callus showed similar characteristics, but embryogenic properties was not seen yet, and 4) the application of tissue culture technique in the propagation of durian cv. Selat needs further comprehensive  investigation, particularly on factors directly affecting culture development and inducing somatic embryogenesis.Key words: tissue culture, in vitro culture, micropropagation, plant hormones, auxin, cytokinin, fruit crops. ABSTRAKPenelitian  ini  bertujuan  untuk  mendapatkan komposisi  media  yang  tepat dari  kombinasi Picloram + BAP untuk proliferasi kalus embriogenik dari eksplan daun dewasa durian. Penelitian dilakukan  di  Laboratorium  Bioteknologi Tanaman,  Fakultas  Pertanian,  Universitas  Jambi  dari Januari hingga November 2012. Perlakuan kombinasi media zat pengatur tumbuh adalah lima taraf Picloram (1.0, 2.0, 3.0, 4.0, 5.0 mg L-1) dengan kombinasi tiga taraf perlakuan BAP (0, 0.5, 1.5 mg L-1).  Terdapat  15  kombinasi  media perlakuan dengan  4  ulangan,  sehingga  terdapat  60  kombinasi satuan percobaan. Setiap unit percobaan terdapat 4 botol kultur dengan satu eksplan daun. Kultur disimpan di ruang kultur selama 16 jam penyinaran dan intensitas cahaya 1000  lux. Hasil penelitian menunjukkan bahwa: 1) Proliferasi kalus dari eksplan daun  muda buah  durian tergantung pada taraf kombinasi  picloram  +  BAP yang  ditambahkan  ke  media  kultur;  2)  Penambahan  3.0-5.0  mg  L-1Picloram  tanpa  BAP  memberikan  hasil  yang  efektif  untuk  menginduksi proliferasi  kalus  pada sebagian besar eksplan; 3)  Regenerasi kalus menunjukkan karakteristik serupa, tetapi embriogenik kalus  tidak  muncul, dan 4)  Perbanyakan  eksplan  daun  durian  dengan  tehnik  kultur jaringan membutuhkan penelitian lebih  lanjut, terutama pada faktor yang berpengaruh langsung pada induksi embriogenesis somatik.Kata kunci: auksin, buah, kultur jaringan, kultur in vitro, mikropropagasi, sitokinin, zat pengaturtumbuh,
Induksi dan Multiplikasi Tunas Jarak Pagar (Jatropha curcas L.) Secara In Vitro , Lizawati; Trias Novita; Ragapadmi Purnamaningsih
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 1 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.045 KB) | DOI: 10.24831/jai.v37i1.1398

Abstract

The conventional propagation of physic nut (Jatropha curcas) is difficult, because it requires a high number of mother plant, which is very limited. In vitro culture is an alternative technique to conventional one to solve the problem.  An experiment was done to obtain the best in vitro culture media for shoot induction and multiplication. This research was separated into two steps, (1) in vitro induction of explant growth, and (2) in vitro shoot multiplication.  Results showed that medium of WPM + 2.0 ppm BAP induced shoot and leaf better than the control.  The highest number of leaf axillary's multiplication was obtained from the medium WPM + 2.0 ppm BAP + 0.1 ppm NAA. Various medium formulations for the induction and multiplication of shoots resulted in highly leaf fall.  The use of DKW + 2.0 ppm BAP + 0.4 ppm TDZ + 3.0 ppm AgNO3 medium has effectively induced shoot multiplication and reduction of dehydrated leaf. Meanwhile, the used of DKW medium supplemented with 5 ppm kinetin resulted in the best shoot elongation.   Key words :  Induction, in vitro, Jatropha curcas, shoot, multiplication
INDUKSI KALUS EMBRIOGENIK DARI EKSPLAN TUNAS APIKAL TANAMAN JARAK PAGAR (Jatropha curcas L.) DENGAN PENGGUNAAN 2,4 D DAN TDZ Lizawati .
Bioplantae Vol. 1 No. 2 (2012): Bioplantae
Publisher : Bioplantae

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Abstract

This studyaims at determine the interactionbetween the different level of 2.4-D andTDZ concentrations, to induction theembryogeniccallus of physic nut. This Research used completely randomized design with factorial treatment pattern, which consists oftwofactors :concentration of2,4 –D, which consists of 5levels : 0.0, 2.5,  5.0,  7.5, 10.0 ppm, and the concentration ofTDZconsists of 5levels: 0.0,  0.5,  1.0, 1.5, 2.0 ppm. The results showes that there are interactions between 2,4-D andTDZ to  formingcallus   ofthe apicalbudexplants of physic nut.The combination of5.0 ppm2,4-D + 1.0ppmTDZand7.5ppm2,4-D + 1.5ppmTDZproducedthe time appearing of callusfastestcompared toother  treatments. The highestpercentage ofcallus formation occuredin thetreatment of2.5ppm2,4-D + 1.0ppmTDZand7.5ppm2,4-D + 1.5ppmTDZ. Whiteyellow color, shinyand friable are characteristicof embryogenic callusproduced on thetreatment of0.0 ppmand 2.5ppm2,4-D with the addition ofTDZata concentration of 0.5ppm, 1.0 ppm, 1.5 ppmand 2.0ppm.   Key words : embryogenic, callus, TDZ
Pengaruh Pemberian Kombinasi Isolat Fungi Mikoriza Arbuskula terhadap Pertumbuhan Vegetatif Tanaman Jarak Pagar (Jatropha Curcas L.) yang Ditanam pada Tanah Bekas Tambang Batu Bara Lizawati Lizawati; Elis Kartika; Yulia Alia; Rajjitha Handayani
Biospecies Vol. 7 No. 1 (2014): Januari 2014
Publisher : Universitas Jambi

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Abstract

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberiankombinasi isolat fungi mikoriza arbuskula (FMA) terhadap pertumbuhan vegetatiftanaman jarak pagar (Jatropha curcas L.) pada tanah bekas tambang batu bara.Rancangan penelitian yang digunakan adalah rancangan acak lengkap (RAL) dengansatu faktor yaitu kombinasi beberapa isolat FMA : yang terdiri dari Glomus-sp 3, 6, 15dan 16 dengan dosis 20 gr per polybag. Hasil penelitian menunjukkah bahwa pemberianisolat FMA Glomus-sp 3, Glomus-sp 15, Glomus-sp 16 masing – masing sebanyak 6.67g diduga merupakan kombinasi terbaik terhadap pertumbuhan vegetatif bibit jarak pagarumur 4 bulan setelah tanam pada tanah bekas tambang batu bara.
Teknologi Percepatan Pertumbuhan Bibit Duku (Lansium domesticum Corr) melalui Aplikasi Fungi Mikoriza Arbuskular [Accelerating The Growth of Duku Seedlings (Lansium domesticum Corr) through the Application of Arbuscular Mycorrhizal Fungi)] Lizawati LIZAWATI; Elis KARTIKA; Elly Indra SWARI; Zul Fahri GANI
Biospecies Vol. 8 No. 2 (2015): Juli 2015
Publisher : Universitas Jambi

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Abstract

The research investigated the influence of four isolates of arbuscular mycorrhizal fungi(AMF) on the growth of duku seedlings. The research used randomized block design with onefactor of some isolates AMF: Glomus-sp 3, 6, 15, and 16 at a dose of 20 g per polybag. The resultsshowed that inoculation of AMF isolates increase the canopy and root growth of the dukuseedlings. Duku seedlings inoculated with mycorrhiza showed higher plant height, stem diameter,dry weight, secondary roots number and length, than the control. Furthermore, mycorrhizalinoculation increased the absorption of nutrients (Phosphor) in the leaves of duku seedlings. Rootstaining results showed that AMF colonization in the duku roots only indicate hyphae; the otherstructures (vesicles, arbuscular and spores) were not detected.