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Journal : Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)

Pertumbuhan Bibit Tanaman Manggis (Garcinia mangostana L.) Setelah Inokulasi dengan Berbagai Galur Agrobacterium rhizogenes1 , Lizawati; Roedhy Poerwanto; , Sobir; Iman Rusmana; Tri Muji Ermayanti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 2 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.098 KB) | DOI: 10.24831/jai.v35i2.1321

Abstract

Growth of mangosteen essentially depends on its root system.  Therefore, it needs technology to obtain stringer mangosteen root system.  The use of Agrobacterium rhizogenes bacterium is an alternative.  The objectives of this experiment were : 1) to find the effective strain of A. rhizogenes bacterium for inoculation of mangosteen seedling root, 2) to find the best inoculation method for inducing mangosteen seedling root.  The materials used in this experiment were ; mangosteen fruit and A. rhizogenes collection from Puslit Biotechnology LIPI Cibinong-Bogor.  The experiment was arranged in completely randomized design with two factorial treatments.  The first factor : 11 strains A. rhizogenes (ATCC-15834, ATCC-8196, R-1000, 07-20001, A4, A4-J, 509, 510, 511, MAFF 01-1724, and control), the second factor : 2 inoculation methods (cutting and dipping).  The results showed that A. rhizogenes  of ATCC-15834, 509, 07-20001, A4, and R-1000 increased stem diameter, plant height, leaf number, lateral and tertiary root number, better than ATCC-8196, MAFF 01-1724, 510, 511, A4-J, and control.  Cutting root method of inoculation resulted in higher live plant percentage compared to dipping root method.   Key words :  Agrobacterium rhizogenes, Garcinia mangostana, inoculation
Induksi dan Multiplikasi Tunas Jarak Pagar (Jatropha curcas L.) Secara In Vitro , Lizawati; Trias Novita; Ragapadmi Purnamaningsih
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 1 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.045 KB) | DOI: 10.24831/jai.v37i1.1398

Abstract

The conventional propagation of physic nut (Jatropha curcas) is difficult, because it requires a high number of mother plant, which is very limited. In vitro culture is an alternative technique to conventional one to solve the problem.  An experiment was done to obtain the best in vitro culture media for shoot induction and multiplication. This research was separated into two steps, (1) in vitro induction of explant growth, and (2) in vitro shoot multiplication.  Results showed that medium of WPM + 2.0 ppm BAP induced shoot and leaf better than the control.  The highest number of leaf axillary's multiplication was obtained from the medium WPM + 2.0 ppm BAP + 0.1 ppm NAA. Various medium formulations for the induction and multiplication of shoots resulted in highly leaf fall.  The use of DKW + 2.0 ppm BAP + 0.4 ppm TDZ + 3.0 ppm AgNO3 medium has effectively induced shoot multiplication and reduction of dehydrated leaf. Meanwhile, the used of DKW medium supplemented with 5 ppm kinetin resulted in the best shoot elongation.   Key words :  Induction, in vitro, Jatropha curcas, shoot, multiplication