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Sensitivity of Harumanis Mango Seed (Mangifera indica L) Solution toward Aeromonas hydrophila Telaumbanua, Siti; Lukistyowati, Iesje; Syawal, Henni
Jurnal Perikanan dan Kelautan Vol 24, No 1 (2019)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.24.1.24-31

Abstract

Research was conducted from October to December 2017, at the labory Examination of Fish Disease, Fisheries and Marine Faculty of Universitas Riau. The Purpose of this study was to determine the sensitivity of harumanis mango seed (Mangifera indica L) as antimicrobial to A. hydrophila, the range of Minimal Inhibitory Concentration (MIC) and the lethal dose (LD50) harumanis manggo seed (M. indica L) solution against to catfish (Pangasius pangasius) (7-9 cm) by immersion. The research method used doses; D1: 100%; D2: 90%; D3: 80%; D4: 70%; D5: 60%; D6: 50%; D7: 40%; D8: 30%; D9: 20% D10: 10%, and K : Control (Oxytetracyclin).The results showed that mango seed solution was sensitive to A. hydrophila bacteri and was able to inhibit A. hydrophila up to 0.09% dose with inhibit zone of 7.31 mm. Minimum Inhibitory Concetrantion (MIC) is 0.1% (1000 ppm) number of colonies 270.66 CFU/ mL.The dose of LD50 is 2115,85 ppm
Profile of Leukocytes Differentiation of Silver Pompano (Trachinotus blochii) That Were Fed Using Chicken Feather Meal in Fish Diet Fermented by Bacillus sp. Ipa, Adha; Lukistyowati, Iesje; Syawal, Henni
Jurnal Perikanan dan Kelautan Vol 24, No 2 (2019)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.24.2.106-113

Abstract

This research was conducted from July-August 2017. The purpose of the research is to know the effect of adding the chicken feather meal in fish food which was fermented by Bacillus sp. The fermented chicken feather meal was added to the food for adjusting the leukocytes differentiation of Silver Pompano (Trachinotus blochii). The method was an experimental method with a Completely Randomized Design (CRD), one factor with six treatments level. The for 180 fishes. This research used six treatments such as, Kn (negative control): without fermented chicken feather meal by Bacillus sp. in the feed and were not infected by Vibrio alginolyticus, Kp (positive control): without fermented chicken feather meal byBacillus sp. in the feed and were infected by V. alginolyticus, P1-P4 respectively: fermented chicken feather meal by Bacillus sp. in the feed 5%, 10%, 15% and 20%. The collected data is; leukocytestotal, leukocytes differentiation, the average of weight, and survival rate. The results showed that there were a significant effect of adding the fermented chicken feather meal by Bacillus sp.  in the feed to the leukocytes total and leukocytes differentiation of Silver Pompano (Trachinotus blochii). The best treatment was 10% of Bacillus sp.(P2) wich was shown by the80,53x103 cells of leukocytes total, 80,33% of lymphocytes, 9,67 % of monocytes, 10.00 % of Neutrophils, , 5,30 g was the average of weight and100 % of survival rate.
Erythrocyte Profile of Pangasius Hypophthalmus Feed With Rhizophora Apiculata Leaf Extract and Maintained In Net Cages Zissalwa, Fricilia; Syawal, Henni; Lukistyowati, Iesje
Jurnal Perikanan dan Kelautan Vol 25, No 1 (2020)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.1.70-78

Abstract

 Ekstrak daun R. apiculata memiliki manfaat sebagai suplemen herbal yang dapat ditambahkan ke dalam pakan, karena mengandung senyawa flavonoid, tanin, saponin, terpenoid, dan steroid yang dapat meningkatkan kesehatan ikan jambal siam (P. hypophthalmus). Penelitian ini telah dilaksanakan pada bulan Agustus sampai Oktober 2019. Tujuan penelitian adalah untuk mendapatkan dosis terbaik ekstrak daun R. apiculata yang ditambahkan ke dalam pakan untuk meningkatkan kesehatan ikan jambal siam yang dilihat dari total eritrosit, kadar hemoglobin, kadar hematokrit, dan morfologi eritrosit. Metode yang digunakan adalah metode eksperimen dengan Rancangan Acak Lengkap (RAL)satu faktor empat taraf perlakuan terdiri dari P0;(tanpa penambahan ekstrak). P1;(1,5 mg/kg). P2;(1,7 mg/kg). dan P3;(1,9 mg/kg) dan dilakukan ulangan sebanyak tiga kali sehingga diperlukan 12 unit percobaan. Pemeliharaan dilakukan selama 60 hari di dalam keramba dengan ukuran 1x1,5x1 m3. Pemeriksaan darah dilakukan sebanyak tiga kali,yaitu  pada awal pemeliharaan, hari ke-30, dan hari ke-60. Dosis terbaik terdapat pada perlakuan P2 (1,7 mg/kg) dilihat dari total eritrosit 2,57x106 sel/mm3, kadar hemoglobin 13,13 g/dL, nilai hematokrit 38,43%, pertumbuhan bobot mutlak 38,93 g, kelulushidupan 97,33%, dan menunjukkan morfologi eritrosit yang normal didukung oleh kualitas air selama penelitian seperti DO 6,4-6,9mg/L, pH 6,1-6,8, Suhu 28,2-29,5 0C, dan Amoniak 0,04-0,08 mg/L.
IDENTIFICATION OF PATHOGENIC BACTERIA OF SELAIS (Ompok hypopthalmus) ARE CAUGHT IN THE KAMPAR RIVER TERATAK BULUH VILLAGE IN RIAU PROVINCE Saragih, Andre A; Syawal, Henni; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract

This research had been conducted in April up to June 2014 in LaboratoryofParasite andFish Diseases,Faculty of Fisheriesand Marine Sciencesof RiauUniversity. This research aims to find out the type of bacteria pathogenicpotentially infect slice of fish in the Kampar River, Teratak Buluh Village in RiauProvince. The adventages of this research is to provide the types of bacterialpathogens that potentially attack the slice of fish in the public water of Riau. Themethod applied in this research is survey methode by taking some samples in thefield ( in Kampar River ) and then analyzed in the laboratory. And the result isthere are five species of bacteria found namely Aeromonas sp, Basillus sp,Pseudomonas sp, Stapyhilococcus sp, and Edwardsiella sp.Keywords: Bacterial pathogens, Fish slice
PENGOBATAN PENYAKIT MAS (Motile Aeromonas Septicaemia) DENGAN EKSTRAK DAUN MANGROVE (Rhizophora sp.) PADA IKAN JAMBAL SIAM (Pangasius hypophthalmus) Mastuti, Rini; Syawal, Henni; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract

ABSTRAKDaun mangrove Rhizophora sp. memiliki kandungan antimikrobial seperti tanin, alkaloid, flavonoid dan saponin. Penyakit Motile Aeromonas Septicaemia disebabkan oleh bakteri Aeromonas hydrophila yang dapat menyebabkan kematian 100 % dalam kurun waktu tiga hari. Tujuan penelitian adalah untuk mengetahui dosis terbaik dari ekstrak daun Rhizophora sp. mengobati penyakit MAS (Motile Aeromonas Septicaemia) dilihat dari gambaran eritrosit ikan jambal siam (Pangasius hypophthalmus).Metode yang dilakukan adalah eksperimen dengan Rancangan Acak Lengkap (RAL) satu faktor empat taraf perlakuan. Ikan uji yang digunakan adalah ikan jambal siam yang terinfeksi A. hydrophila berukuran 13-15 cm, sebanyak 120 ekor. Dosis yang digunakan untuk pengobatan adalah 1300 ppm, 1600 ppm, 1900 ppm, dan kontrol tanpa pengobatan. Pengobatan dengan cara perendaman di dalam larutan ekstrak daun Rhizophora sp. selama 5 menit dilakukan dua kali dengan selang waktu 24 jam. Parameter yang diukur antaralain; total eritrosit, kadar hemoglobin, nilai hematokrit, dan kelulushidupan. Hasil penelitian menunjukkan bahwa ekstrak daun Rhizophora sp. mampu mengobati ikan jambal siam yang terinfeksi MAS (Motile Aeromonas Septicaemia) dengan metode perendaman. Dosis yang terbaik 1600 ppm ditandai dengan rata-rata total eritrosit 290 x104 sel/mm3, kadar hemoglobin 9,30 g/dL, nilai hematokrit 29,66 %, dan kelulushidupan ikan uji pascapengobatan 84,61%. Kata kunci: Rhizophora sp, Aeromonas hydrophila,Hematologi, Pangasius hypophthalmus
ADDITION OF MANGOSTEEN RIND (Garcinia mangostana L.) SIMPLICIA IN FEED ON DIFFERENTIATION OF LEUKOCYTES OF AFRICAN CATFISH (Clarias gariepinus) INFECTED BY Aeromonas hydrophila Syatma, Mario; Lukistyowati, Iesje; Aryani, Netti
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 3, No 1 (2016): Wisuda Februari Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Abstract

This research was conducted on 12nd May until 23rd July 2015. The aims of thisresearch was to determine the best dose of mangosteen rind (Garcinia mangostanaL.) simplicia addition in feed to increase non-specific immune responses in Africancatfish (Clarias gariepinus). This research used Completely Randomized Design(CRD) with one factor, five treatments and three replications. The treatments wereaddition of mangosteen rind simplicia in feed with different concentration i.e : Kn(without addition of mangosteen rind simplicia and without infected with A.hydrophila), Kp (without addition of mangosteen rind simplicia and infected with A.hydrophila), P1 (addition of mangosteen rind simplicia 2 g/kg of feed and infectedwith A. hydrophila), P2 (addition addition of mangosteen rind simplicia 4 g/kg of feedand infected with A. hydrophila) and P3 (addition of mangosteen rind simplicia 6 g/kgof feed and infected with A. hydrophila). The results showed that mangosteen rindsimplicia in feed of treatment P3 on the best dose (6 g/kg of feed) and it can beincrease of non-specific immune responses in African catfish.Key words : Garcinia mangostana L., Clarias gariepinus, A. hydrophila,Simplicia, Differentiation of Leukocytes
SURVIVAL RATE OF Pangasius hypopthalmus THAT ARE IMMERSED IN CURCUMIN (Curcuma domestica V) AND WERE INFECTED BY Aeromonas hydrophila Bertha, Agustina; Siagian, Morina Riauwaty; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 3, No 2 (2016): Wisuda Oktober Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was held in June-November 2015 in Parasites and Fish Diseases Laboratory, Faculty of Fisheries and Marine Science, University of Riau Pekanbaru. This research aimed to determine survival rate of Pangasius hypopthalmus that are immersed in curcumin (Curcuma domestica V) and infected by Aeromonas hydrophila. This research used five treatment such as; Kn (negative control): were not immersed of curcumin and were not infected A. hydrophila, Kp (positive control): were not immersed of curcumin and infected by A. hydrophila), P1: immersed with curcumin 0,5g/L, P2 immersed with curcumin 0,7g/L and P3: immersed with curcumin 0,9 g/L. The fish were immersed with curcumin for two minutes each once a week and they were reared for 28 days. The result showed P1 ( immersion with curcumin solution in a dose of 0,5 g/L) were the best treatment with survival rate of 93,33% and total of leucocytes 16,1167x104 cell/mm3. It was concluded that curcumin can be used for prevention of A. hydrophilla and increase the survival rate of Pangasius hypopthalmus.Key words: Pangasius hypophthalmus, Curcumin, Aeromonas hydrophila
EFFECTS OF FEEDING FEED ENRICHED CURCUMIN TURMERIC (Curcuma domestica Val) OVERVIEW OF THE RED BLOOD CELLSPangasius hypophthalmus Malau, Diana P; Riauwaty, Morina; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was conducted in June-August 2016. The purpose of this study was todetermine the effect of feeding feed enriched curcumin turmeric (Curcuma domestica Val)to the picture of the red blood cells (total erythrocytes, hematocrit, and hemoglobinconcentration) Pangasiushypophthalmus and infected by Aeromonas hydrophilla. Thedesign used was Completely Randomized Design (CRD) with one factor, the level 5treatments and 3 replications. The treatments were feeding enriched curcuminextract withdifferent dose i.e Kn = negative control (feeding without being given curcumin extract andwere not infected byAeromonashydrophilla); Kp = positive control (feeding without beinggiven curcumin extract and infected byAeromonashydrophilla); P1 = addition of curcuminextract in feed at a dose of 0.3 g/kg; P2 = addition of curcumin extract in feed at a dose of0.5 g/kg; and P3 = addition of curcumin extract in feed at a dose of 0.7 g/kg. The resultsshowed that feeding enriched curcuminextract against of Pangasiushypophthalmus at adose of 0.7 g/kg (P3) is the best dose for Pangasiushypophthalmuswith a total oferythrocyte 1.87x106 cells/mm3, hematocrit 35.33 %, and hemoglobin concentration of 7.66g/dl after infected byAeromonashydrophilla and generate growth in absolute weight of 3.66g and a survival rate of 100 %.Keywords:Curcuminextract, Pangasius hypophthalmus, Aeromonas hydrophila, Totalerythrocytes,
HISTOPATHOLOGY LIVER AND KIDNEY OF Clarias gariepinus THAT ARE FED SIMPLISIA MANGOSTEEN RIND (Garcinia mangostana L) Kahfi, Khaiqal El; Riauwaty, Morina; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This study has been conducted on Maret to June 2016 in at Parasite and Fish DiseaseLaboratory, Faculty of Fisheries and Marine Sciences, University of Riau. Theaims of this study was to know changes in liver and kidney tissue structures of catfish(Clarias gariepinus) fed simplisia mangosteen rind. This study used an experimentalmethod and histological of the liver and kidney structure of Clarias gariepinus wereanalyzed by descriptive. The treated fishes were fed in three differentconcentrations of fed simplisia mangosteen rind. Kn: (feed control) the fish were notfed simplisia mangosteen rind, P1: The fish were fed simplisia mangosteen rind 2g/kg, P2: The fish were fed simplisia mangosteen rind 4 g/kg, P3: The fish were fedsimplisia mangosteen rind 6 g/kg and fishes were reared for 60 days. Showed that theabnormalities of liver and kidney such us inflammatory cells, haemorrhage,h yp e r t r h o p y and necrosis. Base on data obtain it can be concluded that the bestconcentration of simplisia mangosteen rind 2 g/kg is able to prevent damage of liverand kidney after given fed simplisia mangosteen rind.Key words : Clarias gariepinus, simplisia mangosteen rind, histopathology,kidney, liver
SENSITIVITAS EKSTRAK DAUN Xylocarpus sp. TERHADAP BAKTERI Pseudomonas sp. Putri, Atika; Syawal, Henni; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was conducted from November 2016 until April 2017 in the Parasites and Fish Disease Laboratory, Faculty of Fisheries and Marine, University of Riau and Integrated Chemical Laboratory, Faculty of Mathematics and Natural Science of Health Muhammadiyah University of Riau. The aims of this research was to determine the sensitivity of Xylocarpus sp. leaf extract on Pseudomonas sp., the range of Minimum Inhibitory Concentration (MIC) of Xylocarpus sp. leaf extract can inhibit the growth of Pseudomonas sp. and know the toxicity of Xylocarpus sp. leaf extract against African catfish, the LC50 test conducted by immersion for 24 hours. This research used an experimental method. The treatment that being used were giving Xylocarpus sp. leaf extract at a concentration of D1 (100%), D2 (90%), D3 (80%), D4 (70%), D5 (60%), D6 (50%), D7 (40%), D8 (30%), D9 (20%), D10 (10%) and Kp : control (cloramphenicol). The results showed showed that Xylocarpus sp. leaf extract can inhibit the growth of Pseudomonas sp. at a concentration of 10% with an average of 8,12 mm zone of inhibition. MIC test showed a concentration of 8,5% average number of colonies of bacteria as much as 248,67 × 108CFU/mL can inhibit the growth of Pseudomonas sp. LC50 Xylocarpus sp. leaf extract against African catfish by immersion for 24 hours in the concentration 8,6% (860 ppm).Key words : Xylocarpus sp., Minimum Inhibitory Concentration, Pseudomonas sp., Sensitivitas, LC50