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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Jurnal Veteriner Jurnal Teknologi Pangan dan Gizi Jurnal Penelitian Pascapanen Pertanian Indonesian Journal of Biotechnology Animal Production : Indonesian Journal of Animal Production Jurnal Medik Veteriner ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research
Denny Widaya Lukman
Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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The Effect of Addition Fermented Dairy-Waste Water Sludge By Aspergillus Niger in Ration on Growth Performance and The Caecal Microbial of Broiler Marlina, Eulis Tanti; Balia, Roostita Lobo Lobo; Lukman, Denny Widaya
ANIMAL PRODUCTION Vol 21, No 1 (2019)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.067 KB) | DOI: 10.20884/1.jap.2019.21.1.690

Abstract

Dairy Wastewater Sludge (DWS) is sediment from milk processing. Nutritional content of DWS can be used as feed ingredients. The effects of nutrition in DWS are tested on the growth of broiler as well as its specific impact on the development of microflora on broiler digestion. The research methods used experimental design. Tapioca by-product (onggok) is used as DWS binder while Aspergillus niger fermentation is applied to improve nutritional content. The rate of addition fermented DWS in the ration was evaluated through measuring weight gain for 35 days and microflora quality in the cecum by counting the number of non-pathogenic and pathogenic bacteria in the cecum at the end of the research period. The research data were analyzed by ANOVA with Duncan's multiple range test. The results showed that the addition of 20% fermented DWS in rations resulted in the highest body weight gain and could suppress the growth of pathogenic bacteria (Salmonella spp., E. coli, and Enterobacteriaceae) in caecal. The ratio of non pathogenic and pathogenic bacteria increased proportional to the addition of fermented DWS levels in the ration.   Different caecum weight of broiler with different fermented DWS levels was a reprentation of microorganism activity in caecum.  This condition can illustrate the good health status of livestock so as to optimize the growth of broiler.
KEBERADAAN KOLIFORM PADA DAGING AYAM SUWIR BUBUR AYAM YANG DIJUAL DRAMAGA BOGOR Zelpina, Engki; Purnawarman, Trioso; Lukman, Denny Widaya
Jurnal Teknologi Pangan dan Gizi Vol 19, No 1 (2020)
Publisher : Widya Mandala Surabaya Catholic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (448.4 KB) | DOI: 10.33508/jtpg.v19i1.2447

Abstract

Penelitian ini bertujuan untuk mengetahui cemaran bakteri koliform pada daging ayam suwir bubur ayam yang dijual Dramaga Bogor. Sampel daging ayam suwir bubur ayam yang digunakan berasal dari pedagang bubur ayam. Sejumlah 15 sampel daging ayam suwir bubur ayam di isolasi terhadap bakteri koliform. Metode yang digunakan adalah cara tuang (pour plate method). Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa jumlah cemaran koliform pada daging ayam suwir bubur ayam yang dijual di Dramaga Bogor adalah 4,25 x 105 ± 2,74 x 105. Cfu/g. Jumlah koliform pada daging ayam suwir bubur ayam melebihi SNI sehingga dapat menimbulkan berbagai gangguan kesehatan masyarakat.
Escherichia coli O157:H7 Resistan Antibiotik Pada Daging Kebab yang Dijual di Sekitar Kampus IPB Dramaga Bogor Devi Yanti Sari; Herwin Pisestyani; Denny Widaya Lukman
Acta VETERINARIA Indonesiana Vol. 9 No. 3 (2021): November 2021
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.9.3.179-186

Abstract

Kebab merupakan salah satu makanan siap saji atau ready to eat (RTE) yang populer di seluruh dunia. Escherichia coli (E. coli) O157:H7 banyak dihubungkan dengan kejadian outbreak foodborne disease pada kebab. Kontaminasi E. coli O157:H7 resistan antibiotik pada kebab dapat menimbulkan masalah kesehatan serius. Penelitian ini bertujuan mengidentifikasi E. coli O157:H7 resistan antibiotik yang diisolasi dari daging kebab yang dijual di sekitar Kampus IPB Dramaga Bogor. Total 43 sampel daging kebab diambil dari seluruh pedagang kebab di sekitar Kampus IPB Dramaga dalam radius 2 km dari batas terluar Kampus. Isolasi dan identifikasi E. coli mengacu pada Standar Nasional Indonesia (SNI) 2897:2008 dari Badan Standardisasi Nasional tentang Metode Pengujian Cemaran Mikroba dalam Daging, Telur, dan Susu, serta Hasil Olahannya. Uji serotyping E. coli O157:H7 menggunakan uji Serologis. Uji resistansi E. coli O157:H7 mengacu pada standar Clinical Laboratory Standards Institute (CLSI) dan dilakukan terhadap 10 jenis antibiotik menggunakan metode Kirby-Bauer disk diffusion. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan enam isolat positif E. coli O157:H7 (31.6%; 6/19) yang resistan terhadap ampisilin, amoksisilin-asam klavulanat, sefotaksim, gentamisin, siprofloksasin, enrofloksasin, kolistin sulfat dengan satu isolat termasuk multidrug resistant (MDR). Semua isolat E. coli O157:H7 masih sensitif terhadap trimethoprim-sulfametoksasol, oksitetrasiklin, dan kloramfenikol.
Seroprevalensi dan Faktor Risiko Rabies pada Anjing di Daerah Endemis Kabupaten Sukabumi Ardilasunu Wicaksono; Abdul Zahid; Etih Sudarnika; Dordia Anindita Rotinsulu; Denny Widaya Lukman; Sri Murtini; Yusuf Ridwan
Jurnal Ilmu Pertanian Indonesia Vol. 26 No. 3 (2021): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.26.3.436

Abstract

Sukabumi District is reported to date as an endemic rabies area in West Java Province. In supporting the rabies control programs, the occurrence of rabies needs to be determined to decrease the number of cases. This research aims to determine the seroprevalence of rabies and identify the risk factors associated with rabies in the district. The research was conducted in Jampang Tengah and Cisolok sub-districts with positive rabies cases on dogs and humans. The blood samples were taken from 191 selected dogs using simple random sampling. Rabies antibody titer was measured using ELISA, and the risk factor was identified using selected dogs’ data classified into intrinsic and extrinsic characteristics. Data were analyzed using the chi-square test and the calculation of the odds ratio. The results showed that the seroprevalence of rabies was 7.9% (4.9-10.9%). The average antibody titer was 0.23 EU with a minimum value of 0.03 EU and a maximum of 0.96 EU. Thus, as high as 7.8% of the population showed rabies seropositivity from the unvaccinated dogs, while among the vaccinated dogs, only 8.0% of the population with rabies protective antibody titer. The significant risk factor of rabies seroprevalence is the typology of raising area (p = 0.003), revealing that the dogs raised in the coastal area had the odds of 5.8 (1.6-21.2) higher to get the rabies seropositivity than the dogs raised in the highland area. Keywords: antibody titre, dogs, rabies, risk factor, seroprevalence
Detection of Mycobacterium avium subspecies paratuberculosis Formula Milk in Bogor Widagdo Sri Nugroho; Minarwati Sudarwanto; Denny Widaya Lukman; Ewald Usleber
Jurnal Teknologi dan Industri Pangan Vol. 19 No. 1 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.407 KB)

Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which is usually associated to Crohn’s disease (CD) in human. The disease shows similarities in clinical signs and pathology characteristic with John’s disease (JD) in ruminants which is infected by MAP. Researchers in Europe, USA, and Australia detected MAP in their dairy products and showed the relationship among MAP, CD, and JD. Meanwhile Indonesia imported milk and milk products from those countries to cover the national demand. This situation keeps MAP as potential-problem in national dairy herd and human health in the future. The aim of this study was to detect MAP in the formula milk for toddler. Fifty samples from five established milk producers were taken on August 2006 at the supermarket in Bogor. Two separate diagnostic methods were used parallel in this study i.e polymerase chain reaction method (PCR) with insertion sequence F 57 as the primer and the Mycobacterial Growth Indicator Tube (MGIT). Neither MAP grew in MGIT after 20 weeks of incubation period but 5 samples were found positive by nested PCR. Although there was no evidence weather MAP grew from the samples, as well as in human to provide data on MAP in Indonesia. Key words: Mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR F57
Deteksi Penyakit Bovine Viral Diarrhea pada Sapi Potong Impor melalui Pelabuhan Tanjung Priok Aditya Primawidyawan; Agustin Indrawati; Denny Widaya Lukman
Acta VETERINARIA Indonesiana Vol. 4 No. 1 (2016): Januari 2016
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.354 KB) | DOI: 10.29244/avi.4.1.7-13

Abstract

Penelitian ini bertujuan untuk melakukan suatu kajian serologis tentang penyakit bovine viral diarrhea (BVD) dan mendeteksi adanya kaitan pemeliharaan kandang sebagai faktor risiko sumber penularan penyakit BVD pada sapi potong impor. Pengujian screening awal mengggunakan ELISA (enzyme linked immunosorbant assay) Antibodi BVD terhadap 100 sampel serum darah sapi, dan ditemukan 63 positif terhadap adanya antibodi anti BVD. Selanjutnya dilakukan pengujian lanjutan ELISA Antigen BVD dan hasilnya seluruh sampel negatif terhadap Antigen BVD. Hasil positif uji ELISA terhadap antibodi BVD mengindikasikan bahwa sampel mengandung antibodi anti BVD akibat pernah terinfeksi oleh virus BVD secara sementara (transient) atau melalui vaksinasi. Berdasarkan dokumen health certificate dari negara asal tidak terdapat informasi yang jelas terhadap perlakuan vaksinasi BVD pada sapi potong impor. Oleh karena itu diperlukan pemeriksaan screening di negara Indonesia untuk mendeteksi dan melakukan usaha preventif mencegah penyebaran di feedlot. Faktor-Faktor yang mempengaruhi kejadian hasil ELISA antibodi positif BVD, terkait dengan penyebaran penyakit BVD selama dalam masa pemeliharaan dan penggemukkan adalah program biosekuriti pada peternakan dengan nilai (OR=3,316; CI=1,380-7,967), dan pengelolaan limbah kandang dalam peternakan dengan nilai (OR=2,667; CI=1,105-6,434). Hasil ini menunjukkan ada asosiasi antara kedua faktor yang ada pada peternakan dengan kejadian penyakit BVD.Kata kunci: BVD, ELISA antibodi dan antigen, faktor risiko. (Detection and Risk Factors Study of Bovine Viral Diarrhea in Cattle Imports at Tanjung Priok Port)This research was a serological study on bovine viral diarrhea (BVD) and also to detect the relevance of maintenance farm management as a risk factor on the spreads of BVD. The initial screening test was performed using antibody capture enzyme linked immunosorbent assay (ELISA) to BVD on 100 cattle blood serum samples. The screening test showed that of 63 samples were positive to BVD antibody and 37 samples were negative to BVD antibody. The next screening test was performed using antigen capture ELISA to BVD and all samples showed negative results on BVD antigen. The results of the ELISA test positive for antibodies to BVD indicates that the samples examined anti-BVD antibodies due to BVD virus had been infected by a temporary (transient) or vaccination. Based on the document health certificate from the country of origin there is no clear information on the treatment of BVD vaccination on imports of beef cattle. So, we need a screening examination in the country of Indonesia to detect and perform preventive measures to prevent the spread in feedlots. Relevant factors that affected the occurrence of positive result on BVD antibody detection was farm biosecurity programs with odds ratio (OR) value of 3.316 and confidential interval (CI) value of 1.380-7.967. Further relevant factor was caging waste management with OR value of 2.667 and CI value of 1.105-6.434. There were statistically significant differences (p<0.05) between farm biosecurity programs and caging waste management related to BVD disease incidence.Keywords: BVD, ELISA antibodies and antigen, risk factors
Deteksi Virus African Swine Fever dari Sampah Makanan Kapal Laut Internasional di Pelabuhan Tanjung Priok Putut Isworo Arimurti; Chaerul Basri; Denny Widaya Lukman
Acta VETERINARIA Indonesiana Vol. 9 No. 2 (2021): Juli 2021
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.9.2.112-119

Abstract

ABSTRAK Sampah makanan kapal dihasilkan dari sampah dapur dan atau restoran yang berasal dari kru kapal atau penumpang. Sampah makanan kapal laut internasional yang diturunkan di Pelabuhan Tanjung Priok berpotensi sebagai media pembawa virus African swine fever (ASF) ke Indonesia. Penelitian ini bertujuan untuk mendeteksi keberadan virus ASF dari sampah makanan kapal laut internasional yang berasal atau transit dari negara tertular ASF di Pelabuhan Tanjung Priok. Jumlah sampel dari setiap negara dihitung secara proporsional dari data jumlah frekuensi kedatangan kapal. Sebanyak 23 sampel pooling sampah makanan yang mengandung daging babi didapatkan dari 23 kapal yang berasal dari 5 negara tertular ASF yaitu China, Hong Kong, Vietnam, Filipina, dan Korea Selatan. Sampel diuji dengan real-time PCR di Balai Besar Uji Standar Karantina Pertanian menggunakan kit ekstraksi dan master mix komersial serta primer King seperti yang direkomendasikan oleh OIE. Hasil penelitian menunjukkan dari total 23 sampel yang diambil sebanyak 2 sampel positif (8,69%) mengandung virus ASF. Sampel positif tersebut berasal dari kapal China dan Filipina. Keberadaan virus ASF pada sampah makanan tersebut menunjukkan indikasi bahwa sampah makanan dari kapal laut internasional dapat menjadi jalur masuknya ASF ke Indonesia. Kata kunci: African swine fever, sampah makanan, kapal laut, real-time PCR ABSTRACT Ship’s food waste was generated from the kitchen and or the restaurant originating from ships crews or passengers. International ship’s food waste that is disposed at Tanjung Priok Port has the potential to carry African swine fever (ASF) to Indonesia. This study is aimed to detect the presence of the ASF virus from international ship’s food waste originating or transiting from ASF-infected countries at Tanjung Priok Port. The number of samples from each country which was calculated proportionally from the data on the frequency of ship arrivals. A total of 23 pooling samples of food waste containing pork were obtained from 23 ships from 5 ASF-infected countries, i.e., China, Hong Kong, Vietnam, Philippines, and South Korea. Samples were tested with real-time PCR at The Center for Diagnostic of Agricultural Quarantine using extraction kits and commercial master mixes and King’s primer as recommended by OIE. The results showed that from total of 23 samples, there were 2 positive samples (8.69%) containing the ASF virus. Those positive samples were from China and Philippines ships. The presence of the ASF virus in food waste indicates that food waste from international ships can be the entry route for ASF to Indonesia.
Seroprevalensi Rabies Pascavaksinasi pada Populasi Anjing di Kawasan Endemik Rabies di Kabupaten Sukabumi Sri Murtini; Dordia Anindita Rotinsulu; Yusuf Ridwan; Denny Widaya Lukman; Abdul Zahid; Ardilasunu Wicaksono; Usamah Afiff; Asah Hilaliah; Risqika Akla Velayati; Etih Sudarnika; Koekoeh Santoso; Agus Wijaya
Acta VETERINARIA Indonesiana Vol. 10 No. 1 (2022): Maret 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.1.87-95

Abstract

Penelitian ini bertujuan untuk mengevaluasi hasil vaksinasi rabies dan membandingkan kenaikan titer antibodi yang terbentuk di Kecamatan Cisolok dan Jampang Tengah sebagai Kawasan endemik rabies Kabupaten Sukabumi dan mengasosiasikan titer antibodi yang terbentuk dengan umur, jenis kelamin, dan cara pemeliharaan anjing yang diperiksa . Sebanyak 211 anjing diamati pada studi ini. Sampel serum prevaksinasi dan pascavaksinasi diambil dan selanjutnya diuji dengan uji ELISA tidak langsung untuk mengetahui titer antibodinya. Data titer antibodi dari masing-masing sampel dianalisis secara deskriptif, sedangkan asosiasi umur, jenis kelamin, dan cara pemeliharaan dengan titer antibodi yang terbentuk pascavaksinasi dihitung menggunakan uji chi-square. Hasil pengujian menunjukkan seroprevalensi rabies di Kecamatan Cisolok dan Jampang Tengah sebelum vaksinasi adalah 17,3% dan 2,6% dengan titer 0,58 EU/mL dan 0,55 EU/mL, sedangkan seroprevalensi setelah vaksinasi menjadi 62,5% dan 93% dengan titer 1,29 EU/mL dan 1,33 EU/mL. Variabel umur, jenis kelamin, dan cara pemeliharaan tidak berasosiasi nyata dengan peningkatan titer antibodi rabies setelah vaksinasi. Hasil studi menyimpulkan bahwa seroprevalensi rabies di kawasan endemik rabies di Kabupaten Sukabumi meningkat setelah dilakukan vaksinasi dari sebelumnya 11% menjadi 75,8%, dengan nilai peningkatan titer (Δ titer) sebesar 1,09 EU/mL.
JSPS-9 Microbiologial Quality of Chicken Carcasses in Bogor Indonesia Based on Campylobater sp. and Salmonella sp. Counts Herwin Pisestyani; Elisabet Tangkonda; Maya Shofa; Surachmi Setyaninigsih; Denny Widaya Lukman; Bambang Pontjo Priosoeryanto; Naoaki Misawa
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (508.528 KB)

Abstract

Unhygienic handling chicken carcasses during slaughtering until selling to costumers can lead to contamination by pathogenic bacteria such as Campylobacter sp., and Salmonella sp. entering human’s body can cause foodborne diseaseThe aims of this study were to detect contamination and enumuration of Campylobacter sp, and Salmonella sp. in chicken carcasses from poultry slauhterhouses and markets in Bogor Indonesia.
Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants.  Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD.  Meanwhile Indonesia imported milk and milk products from those countries to cover national demand.  In the future it will be a potential problem to national dairy he Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Rochman Naim; Ewald Usleber
Forum Pasca Sarjana Vol. 32 No. 2 (2009): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants.  Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD.  Meanwhile Indonesia imported milk and milk products from those countries to cover national demand.  In the future it will be a potential problem to national dairy herd and human health.  The aim of this study is to detect MAP in the growing up milk formula.  Fifty samples from five established distributors were taken in Bogor.  Some diagnostic methods were used parallel in this study, namely Mycobacterial Growth Indicator Tube (MGIT), Herrold’s Egg Yolk enrichment with mycobactine-J (HEYM) and polymerase chain reaction method (PCR) with insertion sequence IS 900 and F 57 as primer.  Neither MAP grew up in MGIT and HEYM after 20 weeks of incubation period. No positive samples were found by conventional PCR using IS 900 and F57 either but 5 samples were detected positive by nested PCR F57.  Although there was no evidence of MAP grew from the samples in this study, the comprehensive and sustainable studies on MAP still should be carried out with more and varied samples, as well as in human to provide data on MAP and to anticipate it in Indonesia.   Key words: mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR
Co-Authors Abdul Zahid Abdulwahed Ahmed Hassan Aditya Primawidyawan Agustin Indrawati Andrijanto Hauferson Angi Anindya Kurniawati Annisa Madyanti Geminastiti Parampasi Asah Hilaliah Bambang Pontjo Priosoeryanto Bambang Sumiarto Cahyaningsih, Umi Chaerul Basri Chandra Utami Wirawati Debby Fadhilah Pazra Devi Yanti Sari Edi Sukmawinata Eko Agus Srihanto Engki Zelpina Erfiandini Eka Puspita Etih Sudarnika Eulis Tanti Marlina Ewald Usleber Fachriyan Hasmi Pasaribu Hadri Latif Hasudungan A Sidabalok Heri Yulianto Herwin Pisestyani I Gusti Made Krisna Erawan I Wayan Suardana I Wayan Teguh Wawan I wayan Teguh Wibawan IETJE WIENTARSIH Inriyanti Assa Kallau, Novalino Harold Geoffrey Kamil Riski Sidik Koekoeh Santoso Kumala Andri Asari Loisa Loisa Manuel da Costa Maya Shofa Minarwati Sudarwanto Mirnawati B Sudarwanto Mirnawati Bachrum Mirnawati Bachrum Sudarwanto Mirnawati Baharudin Sudarwanto Mirnawati Baharudin Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Nada Nursaffana Ramadhani Naoaki Misawa Nararya Adinata Novallino Kallau Nugraha, Arifin Budiman Pierre Dorny Platika Widiyani Putut Isworo Arimurti Rahmat Setya Adji Rakhmi Ros Sari Razali Razali Rinaldi Ghurafa Rismayani Saridewi Risqika Akla Velayati Rochman Naim Roostita L Balia Rotinsulu, Dordia Anindita Siti Gusti Ningrum sri murtini . Surachmi Setiyaningsih Surachmi Setyaningsih Surachmi Setyaninigsih Tangkonda, Elisabet Tarigan, Ronald Trioso Purnawarman Usamah Afiff Wicaksono, Ardilasunu Widagdo Sri Nugroho Wijaya, Agus Wyanda Arnafia Yusuf Ridwan