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ISOLASI DAN IDENTIFIKASI ESCHERICHIA COLI O157:H7 PADA DAGING SAPI DI KABUPATEN BADUNG PROVINSI BALI. ISOLATION AND IDENTIFICATION ESCHERICHIA COLI O157:H7 ON BEEF AT BADUNG REGENCY PROVINCE OF BALI I Wayan Suardana; Bambang Sumiarto; Denny Widaya Lukman
Jurnal Veteriner Vol 8 No 1 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Keamanan bahan pangan merupakan masalah yang amat penting bagi konsumen dan industri pangan. Cemaran bakteri Escherichia coli dan Coliform dianggap sebagai indikator sanitasi dalam proses pengolahan bahan pangan. Pelacakan bakteri patogen dalam pangan juga telah dilakukan secara rutin, termasuk yang bersifat zoonosis seperti Escherichia coli O157:H7. Bakteri ini menghasilkan toksin yang dikenal dengan Shiga toxin. Toksin ini dapat menimbulkan diare berdarah, colitis haemorrhagi dan hemolytic uremic syndrome (HUS) pada manusia. Dalam penelitian ini dipelajari hubungan antara tingkat cemaran dan insidensi Coliform, E.coli, E.coli O157 dan E.coli O157:H7 pada daging sapi. Bakteri pertama ditumbuhkan pada media EMBA, selanjutnya dipupuk pada media SMAC dan diakhiri dengan uji aglutinasi lateks untuk memastikan keberadaan bakteri E.coli O157 dan uji antiserum H7 untuk memastikan isolat yang diisolasi merupakan isolat E.coli O157:H7. Hasil isolasi dan identifikasi terhadap 89 sampel daging sapi diperoleh hasil rata-rata tingkat cemran coliform dan E.coli sebesar 93,01+ 2,64x103 cfu/g dan
Detection of Mycobacterium avium subspecies paratuberculosis Formula Milk in Bogor Widagdo Sri Nugroho; Minarwati Sudarwanto; Denny Widaya Lukman; Ewald Usleber
Jurnal Teknologi dan Industri Pangan Vol. 19 No. 1 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which is usually associated to Crohn’s disease (CD) in human. The disease shows similarities in clinical signs and pathology characteristic with John’s disease (JD) in ruminants which is infected by MAP. Researchers in Europe, USA, and Australia detected MAP in their dairy products and showed the relationship among MAP, CD, and JD. Meanwhile Indonesia imported milk and milk products from those countries to cover the national demand. This situation keeps MAP as potential-problem in national dairy herd and human health in the future. The aim of this study was to detect MAP in the formula milk for toddler. Fifty samples from five established milk producers were taken on August 2006 at the supermarket in Bogor. Two separate diagnostic methods were used parallel in this study i.e polymerase chain reaction method (PCR) with insertion sequence F 57 as the primer and the Mycobacterial Growth Indicator Tube (MGIT). Neither MAP grew in MGIT after 20 weeks of incubation period but 5 samples were found positive by nested PCR. Although there was no evidence weather MAP grew from the samples, as well as in human to provide data on MAP in Indonesia. Key words: Mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR F57
KEBERADAAN KOLIFORM PADA DAGING AYAM SUWIR BUBUR AYAM YANG DIJUAL DRAMAGA BOGOR Zelpina, Engki; Purnawarman, Trioso; Lukman, Denny Widaya
Jurnal Teknologi Pangan dan Gizi Vol 19, No 1 (2020)
Publisher : Widya Mandala Surabaya Catholic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (448.4 KB) | DOI: 10.33508/jtpg.v19i1.2447

Abstract

Penelitian ini bertujuan untuk mengetahui cemaran bakteri koliform pada daging ayam suwir bubur ayam yang dijual Dramaga Bogor. Sampel daging ayam suwir bubur ayam yang digunakan berasal dari pedagang bubur ayam. Sejumlah 15 sampel daging ayam suwir bubur ayam di isolasi terhadap bakteri koliform. Metode yang digunakan adalah cara tuang (pour plate method). Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa jumlah cemaran koliform pada daging ayam suwir bubur ayam yang dijual di Dramaga Bogor adalah 4,25 x 105 ± 2,74 x 105. Cfu/g. Jumlah koliform pada daging ayam suwir bubur ayam melebihi SNI sehingga dapat menimbulkan berbagai gangguan kesehatan masyarakat.
Identifikasi Penambahan Air pada Daging Sapi dengan Metode Filter Paper Press dan Konduktivitas Listrik Nararya Adinata; Denny Widaya Lukman; Etih Sudarnika
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.723 KB) | DOI: 10.22146/jsv.33447

Abstract

Beef meat has a complete nutritional content, the high prices in the market causing some unscrupulous traders to cheat by adding water to meat to get more weight. This beef meat has a bad quality, so needs the right method to identify the quality of beef meat in the field.  This study was designed to identify the addition of water to beef meat by measuring the water holding capacity (filter paper press method) and electrical conductivity (EC meter). The experimental used one hundred bovine longisimus dorsi and the samples were divided into five groups. One group as a control and four other groups were treatment group. The treatment groups were injected by water with a volume of 1%, 2%, 3%, and 4% of the sample weight and all groups were repeated for twenty times. The results were analyzed by ANOVA and Duncan Multiple Range test. The data showed that the addition of water by injection in beef meat of treatment group 2%, 3%, and 4% had a significant influence on water holding capacity and electrical conductivity (p<0.05). Pearson correlation results showed a strong to very strong correlation with value in control group (r=0.704; p<0.01), treatment group 1% (r=0.628; p<0.01), 2% (r=0.700; p<0.01), 3% (r=0.642; p<0.01) and 4% (r=0.760; p<0.01). Based on the result of the study, it is known that a water increase in beef at least 2% of the weight significantly affected water holding capacity and it can be identified by using electrical conductivity meter.
Resistensi Antibiotik pada Salmonella Isolat Sapi Bakalan Asal Australia yang Diimpor Melalui Pelabuhan Tanjung Priok Jakarta (ANTIBIOTIC RESISTANCE OF SALMONELLA ISOLATES FROM AUSTRALIAN IMPORTED FEEDER CATTLES THROUGH TANJUNG PRIOK PORT JAKARTA) Anindya Kurniawati; Denny Widaya Lukman; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Importation of feeder cattle from farms constantly using antibiotics in feed could result in the occurrenceof antibiotic resistant bacteria. A study was therefore conducted to investigate the possible use antibioticsand the antibiotic resistance of commensal Salmonella sppagainst several antibiotics. Salmonella spp.bacteria (n=50)were isolated from 100 samples of feeder cattlesfaeces. Total of 50 Salmonella spp. isolateswere subjected for Salmonella spp. examination and the isolated Salmonella spp. was tested for theantibiotic resistance using 10 antibiotics (ampicillin, cephalotin, erythromycin, tetracycline, streptomycin,chloramphenicol, trimethoprim, trimethoprim-sulfamethoxazole, nalidixic acid, and enrofloxacin) usingdisk diffusion method on Muller-Hinton agar following Clinical and Laboratory Standards Institute (CLSI)guidelines for interpretation. The isolated Salmonella showed resistance towards erythromycin 98%,ampicillin 34%, streptomycin 22%, nalidixic acid 8%, cephalotin 6%, tetracycline 4%, and chloramphenicol2%. There was no resistance against enrofloxacin, trimethoprim, and trimethoprim-sulfamethoxazole. Salmonella spp. bacteria derived from imported feeder cattle which have been resistant to antibiotics arepotential for spreading the antibiotic resistant bacteria to other susceptible animals. Such bacteria canalsotransfer the antibiotic resistant gene to other bacteria in Indonesia which ould be a potential threatfor public and animal healths.
Kemampuan Primer IS900 Dan F57 Mendeteksi Mycobacterium avium Subspesies Paratuberculosis Dengan PCR Konvensional Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Abdulwahed Ahmed Hassan; Ewald Usleber
Jurnal Sain Veteriner Vol 26, No 1 (2008): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.413

Abstract

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The Effect of Addition Fermented Dairy-Waste Water Sludge By Aspergillus Niger in Ration on Growth Performance and The Caecal Microbial of Broiler Marlina, Eulis Tanti; Balia, Roostita Lobo Lobo; Lukman, Denny Widaya
ANIMAL PRODUCTION Vol 21, No 1 (2019)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.067 KB) | DOI: 10.20884/1.jap.2019.21.1.690

Abstract

Dairy Wastewater Sludge (DWS) is sediment from milk processing. Nutritional content of DWS can be used as feed ingredients. The effects of nutrition in DWS are tested on the growth of broiler as well as its specific impact on the development of microflora on broiler digestion. The research methods used experimental design. Tapioca by-product (onggok) is used as DWS binder while Aspergillus niger fermentation is applied to improve nutritional content. The rate of addition fermented DWS in the ration was evaluated through measuring weight gain for 35 days and microflora quality in the cecum by counting the number of non-pathogenic and pathogenic bacteria in the cecum at the end of the research period. The research data were analyzed by ANOVA with Duncan's multiple range test. The results showed that the addition of 20% fermented DWS in rations resulted in the highest body weight gain and could suppress the growth of pathogenic bacteria (Salmonella spp., E. coli, and Enterobacteriaceae) in caecal. The ratio of non pathogenic and pathogenic bacteria increased proportional to the addition of fermented DWS levels in the ration.   Different caecum weight of broiler with different fermented DWS levels was a reprentation of microorganism activity in caecum.  This condition can illustrate the good health status of livestock so as to optimize the growth of broiler.
RESISTENSI Salmonella spp. TERHADAP BEBERAPA ANTIBIOTIK PADA DAGING ITIK DI KABUPATEN BOGOR YANG DAPAT MEMENGARUHI KESEHATAN KONSUMEN (Resistance of Salmonella spp. to Several Antibiotics from Duck Meat in Bogor District that Could Influence Consumer Health) Loisa Loisa; Denny Widaya Lukman; Hadri Latif
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5040

Abstract

This study aimed to determine the presence of Salmonella spp. in duck meat and to identify the resistance of Salmonella spp. against several antibiotics in duck meat associated with food safety. Total of 52 meat samples of ducks was collected from 5 subdistricts in Bogor District, i.e., Cariu-Jonggol, Gunung Sindur, Klapanunggal, Parung Panjang, and Ciomas based on the assumption of 95% confidence level, 50% predicted prevalence, and 10% standard error. The results showed that three samples (5.8%) were positive Salmonella spp. The majority of Salmonella spp. isolated from duck meat showed resistance against erythromycin (66.7%), streptomycin (33.3%), and chloramphenicol (33.3%). Nevertheless, Salmonella spp. was still sensitive againts enrofloxacin, trimethoprim sulfamethoxazole, cephalothin, ampicillin, nalidixid acid, tetracycline, and gentamicin.
5. Analysis of thigh Death Chicken by Shear Values and Impedance Approach Razali Razali; Denny Widaya Lukman
The International Journal of Tropical Veterinary and Biomedical Research Vol 1, No 2 (2016): Vol. 1 (2) November 2016
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.698 KB) | DOI: 10.21157/ijtvbr.v1i2.6687

Abstract

Experiments were conducted to determine whether thigh meat from death chicken can be identified through Warner-Bratzler (WB) shear value and using the impedance value.  Thirty of thigh meat (Biceps femoris) were obtained from commercial slaughtering house classified into three groups namely halal slaughtered healthy chicken (AHS), slaughtered death chicken (AMS), and slaughtered stressed chicken (ALS).  Biceps femoris muscles were used to microscopic procedures and to assess WB score values and impedance values. This study showed that shear values of AMS thigh meat were lower than AHS and ALS after 5 and 9 h PM. This study indicated that the impedance value of AMS were significantly lower (P0.05) than AHS and ALS, it can be used to distinguish between halal thigh and the thigh meat from slaughtered death chicken.
Indirect Enzyme Linked Immunosorbent Assay Sebagai Metode untuk Melacak Bruselosis pada Sapi Perah (INDIRECT ENZYME IMMUNOSORBENT ASSAY (I-ELISA) AS METHOD FOR DETECT BRUCELLOSIS IN DAIRY COW) Rinaldi Ghurafa; Denny Widaya Lukman; Hadri Latif
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.425 KB) | DOI: 10.19087/jveteriner.2019.20.1.30

Abstract

Brucellosis has become a zoonotic disease that received attention in efforts to prevent and eradicate strategic infectious animal diseases in Indonesia. Brucellosis can be detected early by the rose bengal test (RBT), followed by complement fixation test (CFT) and by enzyme linked immunosorbent assay (ELISA). The aims of this research was to study the indirect enzyme linked immunosorbent assay test (I-ELISA) as an alternative test for detecting brucellosis in dairy cattle. The method was used by conducting tests of RBT, CFT, I-ELISA and commercial I-ELISA to test brucellosis. The test results were calculated sensitivity and specificity, as well as analyzed by calculating the kappa value. The method was used by conducting tests of RBT, CFT, I-ELISA and commercial I-ELISA to test brucellosis. The test results were calculated for sensitivity and specificity, as well as analyzed by calculating the Kappa statistical value. The results of the sensitivity and specificity calculation showed that the indirect enzyme linked immunosorbent assay (I-ELISA) test developed a higher sensitivity (100%) compared to RBT test (93.75%) and commercial I-ELISA (93.75%). The developed I-ELISA specificity (74.68%) was still lower than RBT (89.87%), but higher than commercial I-ELISA (70.52%). The calculation of the statistical value of kappa RBT with CFT showed the kappa value 0.7120 which meaned it had a good agreement, commercial I-ELISA with CFT showed kappa value 0.6165 which meaned it had good suitability, whereas I-ELISA developed with CFT showed kappa value 0.4984 which meaned having a moderate agreement.In conclusion, the indirect enzyme linked immunosorbent assay (I-ELISA) which had been developed had low specificity, but the sensitivity was the highest compared to the commercial I-ELISA test and RBT, so this test was appropriate to be used as a screening test, especially in dairy cows movement into brucellosis-free areas or regions.
Co-Authors Abdul Zahid Abdulwahed Ahmed Hassan Aditya Primawidyawan Andrijanto Hauferson Angi Anindya Kurniawati Annisa Madyanti Geminastiti Parampasi Asah Hilaliah Balia, Roostita Lobo Lobo Bambang Pontjo Priosoeryanto Bambang Sumiarto Cahyaningsih, Umi Chaerul Basri Chandra Utami Wirawati Debby Fadhilah Pazra Devi Yanti Sari Edi Sukmawinata Eko Agus Srihanto Elisabet Tangkonda Engki Zelpina Engki Zelpina Erfiandini Eka Puspita Etih Sudarnika Eulis Tanti Marlina Ewald Usleber Fachriyan Hasmi Pasaribu Hadri Latif Hasudungan A Sidabalok Heri Yulianto Herwin Pisestyani, Herwin I Gusti Made Krisna Erawan I Wayan Suardana I Wayan Teguh Wawan I wayan Teguh Wibawan IETJE WIENTARSIH Indrawati, Agustin Inriyanti Assa Kallau, Novalino Harold Geoffrey Kamil Riski Sidik Koekoeh Santoso Kumala Andri Asari Loisa Loisa Manuel da Costa Maya Shofa Minarwati Sudarwanto Mirnawati Bachrum Mirnawati Bachrum Sudarwanto Mirnawati Baharudin Sudarwanto Mirnawati Baharudin Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Nada Nursaffana Ramadhani Naoaki Misawa Nararya Adinata Novallino Kallau Nugraha, Arifin Budiman Nyoman Sadra Dharmawan Pierre Dorny Putut Isworo Arimurti Rahmat Setya Adji Rakhmi Ros Sari Razali Razali Rinaldi Ghurafa Rismayani Saridewi Risqika Akla Velayati Rochman Naim Rotinsulu, Dordia Anindita Siti Gusti Ningrum sri murtini . Surachmi Setiyaningsih Surachmi Setyaningsih Surachmi Setyaninigsih Tarigan, Ronald Trioso Purnawarman Usamah Afiff Wicaksono, Ardilasunu Widagdo Sri Nugroho Wijaya, Agus Wyanda Arnafia Yusuf Ridwan Yusuf Ridwan Yusuf Ridwan