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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Jurnal Veteriner Jurnal Teknologi Pangan dan Gizi Jurnal Penelitian Pascapanen Pertanian Indonesian Journal of Biotechnology Animal Production : Indonesian Journal of Animal Production Jurnal Medik Veteriner ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research

Denny Widaya Lukman

Institut Pertanian Bogor

Author-ID : 309447

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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KEBERADAAN Salmonella sp. PADA DAGING AYAM SUWIR BUBUR AYAM YANG DIJUAL DI LINGKAR KAMPUS INSTITUT PERTANIAN BOGOR DRAMAGA BOGOR Engki Zelpina; Trioso Purnawarman; Denny Widaya Lukman
Jurnal Penelitian Pascapanen Pertanian Vol 15, No 2 (2018): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v15n2.2018.73-79

Penelitian ini bertujuan untuk melakukan studi mengetahui keberadaan Salmonella sp. pada daging ayam suwir bubur serta rekomendasi agar aman dan layak untuk dikonsumsi. Penelitian ini merupakan kajian lapang cross sectional study. Populasi dalam penelitian ini adalah seluruh pedagang bubur ayam yang berada (radius 100 meter) di lingkar kampus Institut Pertanian Bogor, Dramaga, Bogor. Penelitian dilakukan melalui wawancara, observasi, dan pengujian laboratorium. Sampel pada penelitian ini diambil dari 15 pedagang bubur ayam, setiap pedagang diambil sampel sebanyak 3 kali ulangan, total sampel adalah 45. Analisis data dilakukan secara univariat dan bivariat (fisher exact test). Hasil penelitian menunjukkan bahwa ditemukan keberadaan Salmonella enteretica serovars Enteritidis dalam daging ayam suwir bubur ayam sebanyak 6,66% (3/45) dan terdapat hubungan antara asal daging ayam dan keberadaan Salmonella Enteritidis. (p value=0,022 dan CC=0,577). This study was aims to conducted studies to determine the presence of Salmonella sp. in shredded chicken meat of chicken porridge and recommendations for safe for consumption. A cross sectional study approach was used in this study. The population in this study was all chicken porridge seller which located in radius of 100 m around of the campus area Bogor Agricultural University, Dramaga, Bogor. The research was done through interview, observation and laboratory examination. The samples in this study were 15 chicken porridge seller with repeated for three times and total number of samples were 45. The data were analyzed by univariat and bivariate (fisher exact test). The results of this study showed the presence of Salmonella Enteritidis in 6.66% (3/45) of shredded chicken meat chicken porridge and there was a relationship between the origin of chicken meat and the presence of Salmonella Enteritidis (p value = 0.022 and CC = 0.577).

Comparative lactic acid bacteria (LAB) profiles during dadih fermentation with spontaneous and back-slopping methods, as identified by terminal-restriction fragment length polymorphism (T-RFLP) Chandra Utami Wirawati; Mirnawati Bachrum Sudarwanto; Denny Widaya Lukman; Ietje Wientarsih; Eko Agus Srihanto
Indonesian Journal of Biotechnology Vol 26, No 3 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.61164

The diversity of lactic acid bacteria (LAB) present during the manufacture of traditional fermented buffalo milk from West Sumatra, known as dadih, was studied via a culture-independent approach using terminal-restriction fragment length polymorphism (T-RFLP) to compare the dynamic diversity in back-slopping and spontaneous fermentation methods. Total LAB and pH were measured in freshly prepared buffalo milk and in \textit{dadih} fermented for 24 and 48 hours. The results indicated significant differences between the fermentation methods, with higher total LAB, and greater phylotype richness and relative abundance being identified in the back-slopping method. Terminal fragment lengths (TRFs) of 68 and 310 bp were common to both techniques, similar to those of Lactobacillus fermentum, Fructobacillus pseudoficulneus, Leuconostoc citreum, Leuconostoc kimchii, and Leuconostoc sp. The changes in phylotype number (species number) and relative abundances of LAB communities identified are expected to produce data needed to formulate the best fermentation process for dadih manufacturing. A 24-hour back-slopping fermentation method is recommended, as fermentation time of longer than 24 hours reduced viable LAB significantly. Our results also indicated that the T-RFLP technique is not only clearly sensitive enough and adequate for segregating LAB diversity in both fermentation methods, but that it also provides good information regarding the structure of microbial communities and their composition change during the fermentation process.

Kemampuan Primer IS900 Dan F57 Mendeteksi Mycobacterium avium Subspesies Paratuberculosis Dengan PCR Konvensional Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Abdulwahed Ahmed Hassan; Ewald Usleber
Jurnal Sain Veteriner Vol 26, No 1 (2008): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.413

Analisis Hubungan Antara Pengetahuan dan Sikap Terhadap Praktik Penggunaan Antibiotika oleh Peternak Babi di Kota Kupang Provinsi Nusa Tenggara Timur Novalino Harold Geoffrey Kallau; I Wayan Teguh Wibawan; Denny Widaya Lukman; Mirnawati Baharudin Sudarwanto
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The prevalence of antibiotic resistance is an increasing problem due to the use of false and excessive antibiotics. The use of antibiotics in pig farms is increasing and needs to be aware of side effects, it is also influenced by the knowledge, attitudes and practices of farmers. This study is a cross-sectional study using a structured questionnaire interview to analyze the relationship between knowledge and attitudes towards the practice of using antibiotics by pig farmers in Kupang city. A total of 96 respondents pig farmers spread over 6 districts, District of Maulafa (9 villages) District of Alak (12 villages) District of Oebobo (7 villages), District of Kelapa Lima (5 villages), District of Kota Raja (2 villages) and the district of Kota Lama (1 village). The variables observed were individual characteristics, knowledge, attitudes and practices of the use of antibiotics by pig farmers in relation to the occurrence of antibiotic resistance. The relationship between the variables was tested using the Pearson correlation, while the relationship and magnitude of the direct and indirect impact on the observed variables was determined using path analysis. The results showed knowledge has a very significant impact (p <0.01) and indirectly contributes to the level of antibiotic usage practices preventing the occurrence of antibiotic resistance. Attitudes have a significant effect (p <0.05) and directly affect the practice of antibiotics to prevent the use of antibiotic resistance in Kupang. Conclusion of this study shows that knowledge has a major impact on the practice of using antibiotics by pig farmers, so by increasing farmers' knowledge of the facts incidence of antibiotic resistance, the attitude of farmers will increasingly have a positive significant effect on the level of practice use of antibiotics Prevent antibiotic resistance at a pig farm in the city of Kupang.

Identifikasi Penambahan Air pada Daging Sapi dengan Metode Filter Paper Press dan Konduktivitas Listrik Nararya Adinata; Denny Widaya Lukman; Etih Sudarnika
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Beef meat has a complete nutritional content, the high prices in the market causing some unscrupulous traders to cheat by adding water to meat to get more weight. This beef meat has a bad quality, so needs the right method to identify the quality of beef meat in the field. This study was designed to identify the addition of water to beef meat by measuring the water holding capacity (filter paper press method) and electrical conductivity (EC meter). The experimental used one hundred bovine longisimus dorsi and the samples were divided into five groups. One group as a control and four other groups were treatment group. The treatment groups were injected by water with a volume of 1%, 2%, 3%, and 4% of the sample weight and all groups were repeated for twenty times. The results were analyzed by ANOVA and Duncan Multiple Range test. The data showed that the addition of water by injection in beef meat of treatment group 2%, 3%, and 4% had a significant influence on water holding capacity and electrical conductivity (p<0.05). Pearson correlation results showed a strong to very strong correlation with value in control group (r=0.704; p<0.01), treatment group 1% (r=0.628; p<0.01), 2% (r=0.700; p<0.01), 3% (r=0.642; p<0.01) and 4% (r=0.760; p<0.01). Based on the result of the study, it is known that a water increase in beef at least 2% of the weight significantly affected water holding capacity and it can be identified by using electrical conductivity meter.

Indirect Enzyme Linked Immunosorbent Assay Sebagai Metode untuk Melacak Bruselosis pada Sapi Perah (INDIRECT ENZYME IMMUNOSORBENT ASSAY (I-ELISA) AS METHOD FOR DETECT BRUCELLOSIS IN DAIRY COW) Rinaldi Ghurafa; Denny Widaya Lukman; Hadri Latif
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Brucellosis has become a zoonotic disease that received attention in efforts to prevent and eradicate strategic infectious animal diseases in Indonesia. Brucellosis can be detected early by the rose bengal test (RBT), followed by complement fixation test (CFT) and by enzyme linked immunosorbent assay (ELISA). The aims of this research was to study the indirect enzyme linked immunosorbent assay test (I-ELISA) as an alternative test for detecting brucellosis in dairy cattle. The method was used by conducting tests of RBT, CFT, I-ELISA and commercial I-ELISA to test brucellosis. The test results were calculated sensitivity and specificity, as well as analyzed by calculating the kappa value. The method was used by conducting tests of RBT, CFT, I-ELISA and commercial I-ELISA to test brucellosis. The test results were calculated for sensitivity and specificity, as well as analyzed by calculating the Kappa statistical value. The results of the sensitivity and specificity calculation showed that the indirect enzyme linked immunosorbent assay (I-ELISA) test developed a higher sensitivity (100%) compared to RBT test (93.75%) and commercial I-ELISA (93.75%). The developed I-ELISA specificity (74.68%) was still lower than RBT (89.87%), but higher than commercial I-ELISA (70.52%). The calculation of the statistical value of kappa RBT with CFT showed the kappa value 0.7120 which meaned it had a good agreement, commercial I-ELISA with CFT showed kappa value 0.6165 which meaned it had good suitability, whereas I-ELISA developed with CFT showed kappa value 0.4984 which meaned having a moderate agreement.In conclusion, the indirect enzyme linked immunosorbent assay (I-ELISA) which had been developed had low specificity, but the sensitivity was the highest compared to the commercial I-ELISA test and RBT, so this test was appropriate to be used as a screening test, especially in dairy cows movement into brucellosis-free areas or regions.

Keberadaan Bakteri Listeria monocytogenes pada Keju Gouda Produksi Lokal dan Impor (PRESENCE OF LISTERIA MONOCYTOGENES IN LOCAL AND IMPORTED GOUDA CHEESES) Debby Fadhilah Pazra; Trioso Purnawarman; Denny Widaya Lukman
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.719 KB)

Listeria monocytogenes is included in the foodborne pathogen, which has been associated with severaloutbreaks of human listeriosis especially in high risk groups. Listeria monocytogenes could be found inGouda cheeses because of poor hygienic and sanitation practices. In addition, this bacteria could surviveduring the making of cheese and cheese ripening process. The purpose of this study was to identify thepresence of L. monocytogenes in local and imported Gouda cheeses and how the safety level of the Goudacheese against contamination of L. monocytogenes. This study used the conventional method in accordancewith the Bacteriological Analytical Manual, US Food and Drug Administration and Bergey’s Manual ofDeterminative Bacteriology to detect the presence of L. monocytogenes at 15 samples of local Gouda cheeseand 15 samples of imported Gouda cheese sold in supermarkets in Jakarta and Bogor. The results of thisstudy showed that was not found L. monocytogenes in local and imported Gouda cheese. It could be concludedthat is Gouda cheese relatively safe from L. monocytogenes and meets Indonesian National Standard.

Cemaran Escherichia coli pada Tepung Telur yang Diimpor Melalui Pelabuhan Tanjung Priok, dan Resistensinya Terhadap Antibiotik (ESCHERICHIA COLI CONTAMINATION ON EGG POWDER IMPORTED THROUGH PORT OF TANJUNG PRIOK AND ITS RESISTANCE AGAINST ANTIBIOTICS) Kamil Riski Sidik; Denny Widaya Lukman; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.548 KB)

Eschericia coli contamination was found on egg powder imported to Indonesia during microbialexamination. The study was conducted to describe the presence of E. coli and its resistance to antibioticsin egg powder imported to Indonesia through The port of Tanjung Priok. Samples was determined usingcross sectional study and the sample size was calculated based on assumption of confidence level of 95%with margin of error of 10% and predicted prevalence of 50%. Total of 100 egg powder samples wascollected in August 2014 from two exporting countries, Ukraine (whole egg powder, n=30) and India (wholeegg powder, n=40 and egg yolk powder, n=30). Examination was performed by packaging and label inspectionof the product followed by samples collection and testing for bacterial contamination using rapid test andconventional isolation and identification methods. Detected E. coli was isolated and then tested forantibiotic resistance. Examination results showed that 4 samples were positive to E. coli. E. coli isolatesshowed resistance against 6 types of antibiotics and 75% of the isolates had resistance against minimumof 3 types of antibiotics. These conditions should be taken into consideration since antibiotic resistance inE. coli would cause negative impacts on human, animal and environmental health.

Identifikasi Escherichia coli yang Resistan Antibiotik pada Daging Burger yang Dijual di Sekitar Kampus IPB Dramaga Bogor Kumala Andri Asari; Denny Widaya Lukman; Trioso Purnawarman
Jurnal Veteriner Vol 22 No 4 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Penelitian ini bertujuan untuk mengetahui cemaran Escherichia coli dan resistansinya terhadap antibiotik pada sampel daging burger yang dijual pada radius 2 km batas Kampus Institut Pertanian Bogor di Dramaga, Bogor. Sebanyak 35 sampel daging burger diperoleh dari semua pedagang burger (tujuh pedagang). Isolasi dan identifikasi E. coli mengacu pada Standar Nasional Indonesia 2897:2008. Uji resistansi terhadap antibiotik menggunakan metode Kirby-Bauer dengan acuan dari Clinical Laboratory Standards Institute tahun 2018. Uji resistansi antibiotik dilakukan terhadap semua isolat E. coli yang didapat dari semua koloni E. coli yang diisolasi dari sampel daging burger. Antibiotik yang digunakan dalam penelitian ini adalah ampisilin, amoksisilin, streptomisin, oksitetrasiklin, tetrasiklin, sefotaksim, gentamisin, enrofloksasin, asam nalidiksat, kloramfenikol, dan trimetoprim-sulfametoksasol. Data dianalisis secara deskriptif. Hasil pengujian menunjukkan11,4% (4 dari 35 sampel) positif E. coli. Isolat yang diperoleh dari empat sampel ada lima isolat E. coli (100%) yang resistan terhadap antibiotik yang diuji. Resistansi tertinggi ditemukan pada antibiotik streptomisin (100%) dan gentamisin (80%). Dari lima isolat E. coli yang didapat, empat isolat di antaranya telah resistan terhadap tiga atau lebih golongan antibiotik yang dikenal sebagai multi-drug resistant (MDR), dengan pola resistansi yang terdiri atas AMP-AMC-S-CN (ampisilin-amoksisilin-streptomisin-gentamisin), AMP-S-ENR-NA-SXT-CN (ampisilin-streptomisin-enrofloksasin-asam nalidiksat-trimetoprim sulfametoksasol-gentamisin), dan AMP-S-ENR-NA-SXT-OT-CN (ampisilin-streptomisin- enrofloksasin-asam nalidiksat- trimetoprim sulfametoksasol-oksitetrasikilin-gentamisin). Keberadaan E. coli resistan pada daging burger yang termasuk pangan siap santap (ready-to-eat food) dapat menimbulkan risiko kesehatan pada konsumen.

Seroprevalensi Positif Sistiserkosis pada Babi Hutan di Kabupaten Way Kanan, Provinsi Lampung (POSITIVE SEROPREVALENCE OF WILD BOAR CYSTICERCOSIS IN WAY KANAN DISTRICT, LAMPUNG PROVINCE) Heri Yulianto; Fadjar Satrija; Denny Widaya Lukman; Mirnawati Sudarwanto
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.458 KB)

Wild boar is also an intermediate host of Taenia solium, besides domestic pig which is the mainsource of Cysticercus infection in human. Cysticercosis in wild boar has not been reported in Indonesia.The aim of this study was to determine wild boar’s cysticercosis in Way Kanan District. One hundred serasamples consisting of 41 male wild boars and 59 female wild boars were collected. Eighty seven of boarscome from Banjit Subdistrict, while 13 of wild boars come from Blambangan Umpu Subdistrict in WayKanan District. The serum were tested serologically to determine antigen of parasite cycles. The test usedmonoclonal antibody-base sandwich Enzyme-Linked Immunosorbent Assay (Moab-ELISA). There is asero positive reaction of cysticercosis(1%) which is female wild boar from Banjit sub district. Controllingof meat inspection should be conducted to prevent cysticercosis transmission to human.

Co-Authors Abdul Zahid Abdulwahed Ahmed Hassan Aditya Primawidyawan Agustin Indrawati Andrijanto Hauferson Angi Anindya Kurniawati Annisa Madyanti Geminastiti Parampasi Asah Hilaliah Bambang Pontjo Priosoeryanto Bambang Sumiarto Cahyaningsih, Umi Chaerul Basri Chandra Utami Wirawati Debby Fadhilah Pazra Devi Yanti Sari Edi Sukmawinata Eko Agus Srihanto Engki Zelpina Erfiandini Eka Puspita Etih Sudarnika Eulis Tanti Marlina Ewald Usleber Fachriyan Hasmi Pasaribu Hadri Latif Hasudungan A Sidabalok Heri Yulianto Herwin Pisestyani I Gusti Made Krisna Erawan I Wayan Suardana I Wayan Teguh Wawan I wayan Teguh Wibawan IETJE WIENTARSIH Inriyanti Assa Kallau, Novalino Harold Geoffrey Kamil Riski Sidik Koekoeh Santoso Kumala Andri Asari Loisa Loisa Manuel da Costa Maya Shofa Minarwati Sudarwanto Mirnawati B Sudarwanto Mirnawati Bachrum Mirnawati Bachrum Sudarwanto Mirnawati Baharudin Sudarwanto Mirnawati Baharudin Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Nada Nursaffana Ramadhani Naoaki Misawa Nararya Adinata Novallino Kallau Nugraha, Arifin Budiman Pierre Dorny Platika Widiyani Putut Isworo Arimurti Rahmat Setya Adji Rakhmi Ros Sari Razali Razali Rinaldi Ghurafa Rismayani Saridewi Risqika Akla Velayati Rochman Naim Roostita L Balia Rotinsulu, Dordia Anindita Siti Gusti Ningrum sri murtini . Surachmi Setiyaningsih Surachmi Setyaningsih Surachmi Setyaninigsih Tangkonda, Elisabet Tarigan, Ronald Trioso Purnawarman Usamah Afiff Wicaksono, Ardilasunu Widagdo Sri Nugroho Wijaya, Agus Wyanda Arnafia Yusuf Ridwan

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