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All Journal Jurnal Perlindungan Tanaman Indonesia
Y. Andi Trisyono
Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
Agriculture, Biological Sciences & Forestry

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Benefits of Flowering Plant as Refuge to Improve the Ecosystems Services by Egg Parasitoids of the Rice Brown Planthopper Nike Grace Hanjelina Sinulingga; Y. Andi Trisyono; Edhi Martono; Buyung Hadi
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.28536

Abstract

Outbreaks of the rice brown planthopper, Nilaparvata lugens Stal., tend to increase in 2017. There has been significant interest to reduce reliance on pesticides by manipulating habitat plant species and communities to benefit natural enemies of insect. Flowering plants as refuge can contribute in enhancing the ecosystem services. This study aimed to assess the benefit of flowering plants as refuge to improve the role of egg parasitoids of brown planthopper. We sampled three rice fields: rice field adjacent to refuge, far from refuge, and rice field with no refuge using trapping procedure. We found two genera of parasitoid in Banyumas: Oligosita and Anagrus. The parasitism in the three rice fields was 46.14, 43.05 and 42.32%, respectively, showing no differences. However, the number of parasitoids emerged from the traps placed in the rice field with refuge was higher (31.08 adults/trap) than the other two rice fields (25.67 and 20.71 adults/trap). In addition, the number of unhatched parasitoids was lower in the rice with refuge (5.9%) compared to no refuge (14.54%). These findings show that the refuge provides better environments for the parasitoid by improving the number of progeny produced which eventually could increase their role in managing N. lugens population.
The Knowledge and Attitude of Integrated Pest Management Farmers Field Schools Alumni toward the Use of Pesticides in Klaten, Central Java, Indonesia Andriarti Kusumawardani; Edhi Martono; Y. Andi Trisyono; Nugroho Susetya Putra
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.32098

Abstract

The field schools in Indonesia employ an extension alternative model that is considered appropriate to change the habits and behavior of farmers to make them IPM experts in their field. This study was conducted to understand the behavior (knowledge and attitude) of farmers who have joined field schools, i.e. Integrated Pest Management Farmers Field School (IPM-FFS) and Landscape Integrated Pest Management (Landscape IPM), toward the use of pesticides in Klaten, Central Java, Indonesia. The research used a survey method with purposively selected respondents comprising 55 farmers of the IPM alumni and 25 farmers of the Landscape IPM alumni. A descriptive analysis was conducted to analyze the farmers’ knowledge and attitude toward the use of pesticides. Mann-Whitney U-test analysis was performed using Statistical Product and Service Solution (SPSS) version 23, to compare the knowledge and attitude of farmers regarding the use of pesticides. The results showed that the knowledge of farmers of  the Landscape IPM alumni regarding the use of pesticides was higher than that of farmers of the IPM-FFS alumni. There was no difference in the behavior of farmers between the IPM-FFS alumni and the Landscape IPM alumni regarding their attitude toward the use of pesticides.
Detection and Analysis of Protein Profile on Rice Infected by Stunting Virus with Different Severity on Ciherang and Situ Bagendit Varieties Selvi Helina; Sri Sulandari; Sedyo Hartono; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.36549

Abstract

Rice stunt virus is one of the limiting factors in the decline of rice production in Indonesia. The virus consists of rice grassy stunt virus (RGSV) and rice ragged stunt virus (RRSV) that is transmitted by brown planthopper (WBC) in a persistent propagative manner. This study aimed to determine the presence of rice stunt virus in Bantul, Yogyakarta through fast detection using RT-PCR. It also aimed to learn the pattern of total protein profile of healthy and infected rice plants by the virus on different severity level in the field. The results showed that rice varieties of Ciherang and Situ Bagendit in Bantul were infected with RGSV and RRSV. They were classified as mild, moderate, severe, and failure in severity level. Homology analysis using BioEdit showed that the nucleotide sequence of RGSV in Bantul isolate had the highest percentage of nucleic acids similarity with Klaten isolate (98.1%). Meanwhile, RRSV of Bantul isolate had the highest percentage of nucleic acids similarity to Philipines isolate (99.5%). Analysis of protein profiles using SDS-PAGE showed a pattern of protein profiles formed on rice infected with the virus at different severity levels which was not found in healthy rice. These proteins presumably were nonstructural p5 and nucleocapsid protein (NCP) of RGSV with a molecular weight of ~22 and 34-35 kDa; and viral spike protein and protein capsid (S8) of RRSV with MW ~39 and ~43 kDa.
Attraction of Yellow Stem Borer (Scirpophaga incertulas Walker) to Different Colored Light-emitting Diodes Yhone Arialistya; Suputa Suputa; Y. Andi Trisyono; Eka Firmansyah
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.37535

Abstract

The yellow rice stem borer (Scirpophaga incertulas Walker) is a major pests on rice. Light traps have been used for monitoring and controlling S. incertulas with white and ultraviolet color light sources. The purpose of this research was to invent trap which uses an effective color that attracts S. incertulas. This research begins by sampling female S. incertulas pre-oviposition from the field then tested in the laboratory. The experiment was designed with factorial randomized block design with light colors and light intencity as factors. Light-emitting diodes used in this research, consisted of normal red, normal yellow, normal green, superbright red, superbright yellow, superbright green, superbright blue, UV, and white. LED white, UV, and superbright blue were attractive colors to S. incertulas  when exposure time was 15 minutes. Effective light intencity to attract S. incertulas was 1000 lux.
Selecting Primers for RAPD, Microsatellite and Mitochondrial Cytochrome Oxidase Subunit 1 for Genetic Variation Analysis of Asian Corn Borer (Ostrinia furnacalis Guenée) Population in Java, Indonesia Ahmad Taufiq Arminudin; Y. Andi Trisyono; Arman Wijonarko; Suputa Suputa
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.38285

Abstract

Primer plays an important role in studying genetic diversity of an insect species. This research was aimed to select the suitable primers to visualize the genetic diversity of Asian corn borer (Ostrinia furnacalis) using Random Amplified Polymorphic DNA (RAPD), microsatellite, and mitochondrial cytochrome oxidase subunit 1 gene (mtCO1). Twenty four RAPD primers (OPA1, OPA4, OPA7,OPA8, OPA10, OPA11, OPA12, OPA13, OPB7, OPB10, OPB11, OPB12, OPB15, OPC4, OPC5, OPC14, OPC16, OPC18, OPC20, OPD3, OPD8, OPD10, OPD13, OPD14) and five microsatellite primers (T3, T4, T5, T81, D25) resulted high polymorphic informations of the genetics of O. furnacalis in Java Indonesia. Universal primers, Lep and Heb were appropriateto do molecular identification of O. furnacalis based on BLAST system on GenBank and BOLD systems.
Detection and Development of Infestation Rate of Aphelenchoides besseyi on Various Rice Seed Varieties Nikmatul 'Azizah; Siwi Indarti; Ani Widiastuti; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.45558

Abstract

Aphelenchoides besseyi is a seed borne nematode caused white tip disease. Infested rice seeds are beneficial inoculum source forA. besseyi widespread. The objective of this research was to determine the occurence and development of symptom level caused by A. besseyi in rice seed varieties. The research was done by detecting A. besseyi in rice seed using Hoshino and Togashi method and counting the nematode population in the seed; observing the incubation time and measuring disease incidence every week. The result revealed that A. besseyi was found in 16 of 17 varieties, i.e. R1 IR64, R2 IR64, R3 IR64, R1 Situbagendit, R2 Situbagendit, Inpari 33, R1 Memberamo, R2 Memberamo, R3 Memberamo, R1 Way Apo Buru, R2 Way Apo Buru, Mekongga, Pepe, Ketan, Sintanur, and Ciherang.A. besseyi infection increased every week in many varieties depend on quantitative (statistics) and qualitative (symptom) indicators. There were 4 varieties have to be investigate further because they were infested by A. besseyi>30 nematodes/100 seeds, i.e. R1 Way Apo Buru, R1 Situbagendit, Ketan and R3 IR 64. A practice that is also required is the elimination of A. besseyi inoculum in rice seed before cultivate.
Farmers’ Practices in Using Insecticides to Control Spodoptera exigua Infesting Shallot Allium cepa var. aggregatum in the Shallot Production Centers of Java Gracia Melsiana Aldini; Y. Andi Trisyono; Arman Wijonarko; Witjaksono Witjaksono; Herman de Putter
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.47893

Abstract

The beet armyworm, Spodoptera exigua Hübn. (Lepidoptera: Noctuidae) is a major insect pest of shallot in Java. This research was aimed to determine how insecticides were used as the common farmers practice  for controlling S. exigua. The research was conducted in three shallot production centers in Java: the Districts of Brebes (Central Java Province), Nganjuk (East Java Province), and Bantul (the Special Region of Yogyakarta).  Surveys were conducted by interviewing thirty shallot farmers as respondents from each district. The selected thirty farmers were taken from  four sub-districts. Almost all farmers (93.3%) in the three districts had similar perception that S. exigua was the major insect pest in shallot and 84.4% farmers stated that this insect was difficult to control. The four most common insecticides active ingredients  used were chlorfenapyr, methomyl, chlorpyrifos, and emamectin benzoate. Insecticides remained the first choice and they were applied throughout the shallot season mostly based on the calendar (1-3 days interval). When using insecticides farmers tend to exceed the label recommended rates, and the farmers mostly mixed different insecticides into one spray solution. These results suggest that application of insecticides to control S. exigua was already exessive. The potential risks and efforts essential to minimize the risks are discussed. 
Polyandry in the Fruit Fly Bactrocera dorsalis Hendel (Diptera: Tephritidae) Defiana Prastiti; Suputa Suputa; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.61108

Abstract

The Oriental fruit fly, Bactrocera dorsalis (Hendel) is a global pest of a wide variety fruits. Due to its importance, the sterile insect technique (SIT) has raised attention as a safe and sustainable solution to this pest. Successful SIT programs require a comprehensive understanding on the mating behavior of this species. Females of oriental fruit flies are known monandrous which implies that females only mate once with one male. This experiment aimed to confirm that female flies were able to mate more than once with the same (multiple mating) or different males (polyandry) in certain period of time. Four different experiments were conducted to test the hypothesis: receptivity a female to the same male (1) and different males (2) in a cage for 11 days; receptivity a mated female with the same male (3) and different males (4) in a cage for 14 days after the first mating. Results indicated that a part of the female oriental fruit flies was capable to mating more than once with the same or different males.
Co-Authors Ahmad Taufiq Arminudin Andriarti Kusumawardani Ani Widiastuti Arman Wijonarko Buyung Hadi Defiana Prastiti Edhi Martono Eka Firmansyah Gracia Melsiana Aldini Herman de Putter Nike Grace Hanjelina Sinulingga Nikmatul 'Azizah Nugroho Susetya Putra SEDYO HARTONO Selvi Helina Siwi Indarti Sri Sulandari Suputa Suputa Witjaksono Witjaksono Yhone Arialistya