Maria Inge Lusida
Tropical Disease Diagnostic Center (TDDC) – Institute of Tropical Disease, Universitas Airlangga.

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The Prevalence of Human Immunodefiency Virus-1 (HIV-1) Subtypes and Transmission Method among HIV/AIDS Infection Patient in Tulungagung, East Java Indonesia Nasronudin, Nasronudin; Lusida, Maria Inge; Witaningrum, Adiana Mutamsari; Kotaki, Tomohiro; Khairunisa, Siti Qamariyah; Utomo, Budi; Widiyanti, Prihartini; Rahayu, Retno Pudji; Juniastuti, Juniastuti; Qushay, M.; Ardianto, Achmad
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 5 (2015)
Publisher : Institute of Topical Disease

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The rapid epidemic growth of HIV is continuing in Indonesia. There are some factors which have influenced the spreading of this epidemic in Indonesia, such as the poor awareness to avoid unsafe free sex attitude and the sharing of needles and syringes among intravenous drug users (IDUs). The sexual transmission of HIV has also apparently increased in Tulungagung. Commercial sex workers play a significant role in the spread of HIV in Tulungagung. People in Tulungagung have worked at other countries as Indonesian migrants. This condition can cause the increase number of HIV-1 case and the possibility of genetic variation (subtype) HIV-1 in Tulungagung. This research is aimed to analyze the subtype and to determine estimation of transmission mode on infected patient of HIV-1 and AIDS who came to Seruni clinic Dr. Iskak hospital in Tulungagung. 40 HIV?AIDSpatients were interviewed to determine the subtype and the transmission mode. The results showed that 14 of 40 plasma samples (35%) were successfully to amplified and sequenced. OverallCRF01-AE wereidentified as predominant subtype among HIV/AIDS patients in Tulungagung. Based on individual information, 31 of 40 subjects (77%) were heterosexual transmission.
QUICK DIAGNOSIS OF JAPANESE ENCEPHALITIS FOR NEW DIAGNOSED EMERGING DISEASE USING PCR TECHNIQUE IN SURABAYA, INDONESIA Jannah, Lilis Mundri; Fajar, Nur Syamsiatul; Nastri, Aldise Mareta; Lusida, Maria Inge; Aksono, Eduardus Bimo; Nasronudin, Nasronudin; Matondang, Muhammad Qushai Y.
Indonesian Journal of Tropical and Infectious Disease Vol 4, No 4 (2013)
Publisher : Institute of Topical Disease

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Background: Japanese enchepalitis (JE) is a viral disease that considered as zoonotic disease, which transmitted through mosquito vectors that had JE virus. Mainly caused by the mosquito C. Tritaeniorhynchus (the most important vector is the mosquito Culex, which feeds on cattle in preference to human). JE virus disease can also cause disturbances in the central nervous system eg. brain, bone marrow, and meninges which has serious impact on public health. This disease has been reported from Japan, Korea, Taiwan, India, Myanmar, Thailand, Western Pacific and Southeast Asia to Indonesia. However, the incidence of this disease in Indonesia has not been well known in various animal species or humans. Aim: The purpose of this study is to develop rapid diagnostic examinations on patient diagnosed JE virus in Surabaya by using PCR (Polymerase Chain Reaction). Because, JE disease can lead to dead-end at the patient if not treated immediately. Method: The research methods, extraction method, PCR (1st RT-PCR and 2nd Nested PCR) are conducted using Japanese encephalitis PCR detection kit. Result: The results of the examination showed that 2 out of 17 people (11,765%) are positive with PCR bands 227 bp (basepair). This diagnostic technique to determine and to deal with early onset of the disease. Solutions for preventive actions can be started from the termination of the cycle vectors to vaccination measures. Conclusion: For his own medical factors given to reduce fever and swelling and reduce the pain.