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All Journal Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Jurnal Ilmiah Aplikasi Isotop Radiasi Journal of Tropical Soils Jurnal Hortikultura Biotropika Journal of Degraded and Mining Lands Management Jurnal Agrotek Lestari Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) SEAS (Sustainable Environment Agricultural Science)
Sukmadewi, Desak Ketut Tristiana
Warmadewa University
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Electrical & Electronics Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology

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Uji Potensi Bakteri Penghasil Hormon IAA (Indole Acetic Acid) dari Tanah Rhizosfer Cengkeh (Syzigium aromaticum L.) Sukmadewi, Desak Ketut Tristiana; Suharjono, Suharjono; Antonius, Sarjiya
Biotropika: Journal of Tropical Biology Vol 3, No 2 (2015)
Publisher : Biotropika: Journal of Tropical Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (468.932 KB)

Abstract

ABSTRAK Tanaman cengkeh (Syzygium aromaticum L.) merupakan salah satu komoditi perkebunan yang memiliki peranan penting bagi pendapatan devisa negara. Perkebunan cengkeh rutin mengunakan pupuk kimia sintetik dalam proses pertumbuhannya. Permintaan produk pertanian dan perkebunan yang bebas akan bahan kimia semakin meningkat. Tujuan dari penelitian ini adalah mempelajari potensi isolat  bakteri penghasil hormon IAA dari tanah rhizosfer tanaman cengkeh. Tahapan penelitian meliputi pengambilan sampel tanah, isolasi bakteri penghasil hormon IAA dan analisa hormon IAA. Bakteri yang berpotensi menghasilkan hormon IAA tertinggi adalah isolat TCKI 5 (32,84 ppm) dari Karangasem. Hasil tertinggi ini didapatkan pada waktu inkubasi 48 jam. Berdasarkan nilai OD terlihat bahwa isolat TCKI 5  pada waktu inkubasi  48 dan 72 jam memiliki nilai OD yang  sama yang  merupakan  fase stasioner   Kata kunci: bakteri, tanaman cengkeh, Indole Acetic Acid (IAA), rhizosfer 
Uji Fitopatogenitas, Hemolisis serta Kemampuan Mikrob dalam Melarutkan Fosfat dan Kalium: Test of Phytopathogenicity, Hemolysis and Microbial Ability in Solubilizing Phosphate and Potassium Desak Ketut Tristiana Sukmadewi; Iswandi Anas; Rahayu Widyastuti; Ania Cintaresmini
Jurnal Ilmu Tanah dan Lingkungan Vol 19 No 2 (2017): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (535.167 KB) | DOI: 10.29244/jitl.19.2.68-73

Abstract

Soil microbes have an important role in the cycle of phosphorus and potassium. Therefore, a multifunctional microbe is required to have two or more functions. Before a multifunctional test is performed, the microbes must be tested and confirmed, they are not pathogenic. The purpose of this research was to study phytopathogenicity, hemolysis and microbial ability in solubilizing phosphate and phosphate. The research procedure consisted of phytopathogenicity test, hemolysis test, the test of phosphate solubilizing ability on Pikovskaya solid medium and potassium solubilizing ability on Alexandrov solid medium. Based on the results of phytopatgonecity tests on bacteria and fungi, all isolates are non-phytopathogenic. From the hemolysis test of bacterial isolate BPK 2, BPK 6 and BPK 7 caused total hemolysis. Based on the hemolysis test of fungi isolate SSIO 6 caused total hemolysis, FPF E1 and JK 6 caused partial hemolysis. Isolate BPK 5 has the highest index in solubilizing potassium (1.375), while isolate BPF 9 has the highest index in solubilizing phosphate (1.533). Keywords: Microbe, multifunctional, pathogenicity, phosphate solubilizing, potassium solubilizing
Eksplorasi Bakteri yang Berpotensi sebagai Pengendali Hama Ulat Grayak (Spodoptera litura) (Exploration of Potential Bacteria as Biological Control of Spodoptera litura) Wilhelmus Terang Arga Sanjaya; Desak Ketut Tristiana Sukmadewi; Fahrizal Hazra; Aisamrotul Hasanah; Dwi Andreas Santosa
Jurnal Hortikultura Vol 30, No 2 (2020): Desember 2020
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v30n2.2020.p133-140

Abstract

Usaha pengendalian hama ulat grayak (Spodoptera litura) di tingkat petani masih mengandalkan pestisida sintetik. Tujuan penelitian adalah mengeksplorasi bakteri potensial pengendali hama ulat grayak (S. litura) dan menguji ketahanan bakteri potensial pada bahan pembawa kompos dan zeolit. Isolat tanah diisolasi dari tiga jenis sumber, yaitu sampel tanah daerah rhizosfer (padi, kelapa sawit, terung, jagung), sampel buah busuk (kakao, kelapa sawit, jambu air), dan sampel bangkai serangga (ulat api, belalang, kumbang tahi, kupu-kupu) yang diambil dari kawasan Dramaga dengan metode purposive sampling. Penelitian di laboratorium meliputi isolasi bakteri, uji patogenitas, pewarnaan gram, pengamatan morfologi koloni, uji toksisitas, uji biokimia, dan uji bahan pembawa. Berdasarkan penelitian ini didapatkan dua strain yang berpotensi sebagai agens biokontrol dengan kemampuan membunuh hama yang tinggi pada pengujian toksisitas tahap kedua, yaitu IRJ 10 (tingkat kematian 90%) dan ISU 4 (tingkat kematian 100%). Kedua isolat ini merupakan anggota genus Bacillus. Pada uji bahan pembawa kompos dan zeolit, penurunan jumlah sel bakteri pengendali hama paling tinggi adalah pada bahan pembawa zeolit dibandingkan dengan menggunakan bahan pembawa kompos. Jumlah sel bakteri pengendali hama pada masa penyimpanan 3 minggu masih di atas 108 CFU/g.KeywordsBakteri; Bahan pembawa; Ekplorasi; Agens pengendali hama; Ulat grayak (Spodoptera litura)AbstractThe effort to control the Spodoptera litura at the farm level still used synthetic pesticides. This research aimed to explore potential bacteria as biological control of S. litura and do viability test of potential bacteria on compost and zeolite carrier. Soil potential bacteria had been isolated from three sources, including rhizosphere soil samples (rice, oil palm, eggplant, corn), rotten fruit samples (cocoa, oil palm, water), and insect samples (fireworms, locusts, dung beetles, butterflies) taken from Dramaga area with the purposive sampling method. Stages of laboratory study include isolation of bacterial isolates, pathogenicity tests, gram staining, colony morphology observation, toxicity test, biochemical test, and viability test. Two strains that have potential as biocontrol agents with a high ability to kill pests in the second stage of toxicity testing are IRJ 10 (90% mortality rate) and ISU 4 (100% mortality rate). Both of these isolates are members of the genus Bacillus. The highest number of viability was found in zeolite carriers. The number of bacterial cells in the three-week storage period is still above 108 CFU/g.
Uji Potensi Bakteri Penghasil Hormon IAA (Indole Acetic Acid) dari Tanah Rhizosfer Cengkeh (Syzigium aromaticum L.) Desak Ketut Tristiana Sukmadewi; Suharjono Suharjono; Sarjiya Antonius
Biotropika: Journal of Tropical Biology Vol 3, No 2 (2015)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK Tanaman cengkeh (Syzygium aromaticum L.) merupakan salah satu komoditi perkebunan yang memiliki peranan penting bagi pendapatan devisa negara. Perkebunan cengkeh rutin mengunakan pupuk kimia sintetik dalam proses pertumbuhannya. Permintaan produk pertanian dan perkebunan yang bebas akan bahan kimia semakin meningkat. Tujuan dari penelitian ini adalah mempelajari potensi isolat  bakteri penghasil hormon IAA dari tanah rhizosfer tanaman cengkeh. Tahapan penelitian meliputi pengambilan sampel tanah, isolasi bakteri penghasil hormon IAA dan analisa hormon IAA. Bakteri yang berpotensi menghasilkan hormon IAA tertinggi adalah isolat TCKI 5 (32,84 ppm) dari Karangasem. Hasil tertinggi ini didapatkan pada waktu inkubasi 48 jam. Berdasarkan nilai OD terlihat bahwa isolat TCKI 5  pada waktu inkubasi  48 dan 72 jam memiliki nilai OD yang  sama yang  merupakan  fase stasioner   Kata kunci: bakteri, tanaman cengkeh, Indole Acetic Acid (IAA), rhizosfer 
Peningkatan Kemampuan Mikroba Pelarut Fosfat dan Kalium Melalui Teknik Mutasi Iradiasi Gamma Desak Ketut Tristiana Sukmadewi; Iswandi Anas; Rahayu Widyastuti; Ania Citraresmini
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 15, No 2 (2019): Desember 2019
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.198 KB) | DOI: 10.17146/jair.2019.15.2.4662

Abstract

Iradiasi gamma merupakan salah satu alternatif untuk memicu mutasi yang dapat menginduksi peningkatan kemampuan mikroba pelarut fosfat dan kalium. Tujuan dari penelitian ini adalah  untuk mempelajari pengaruh iradiasi gamma terhadap mikroba pelarut fosfat dan kalium, mempelajari perubahan kemampuan mutan mikroba dalam  melarutkan fosfat dan kalium, serta perubahan pada tingkat molekuler yang terjadi akibat mutasi iradiasi gamma Metode penelitian terdiri dari iradiasi mikroba menggunakan sinardengan dosis 0; 1; 2,5; 5; 7,5; 10; 15 kGy, uji kemampuan mikroba dalam melarutkan fosfat dan kalium setelah iradiasi serta pengujian pada tingkat molekuler. Hasil yang didapatkan pada penelitian ini adalah iradiasi gamma  memberikan pengaruh terhadap jumlah populasi dan kemampuan mikroba dalam melarutkan fosfat dan kalium. Hal ini menunjukkan bahwa sinar gamma dengan dosis 1 kGy sampai 15 kGy menurunkan populasi bakteri dan fungi. Semakin tinggi dosis iradiasi gamma jumlah sel yang mati meningkat. Umumnya iradiasi dengan sinar gamma menghasilkan mutan dengan kemampuan melarutkan P dan K yang menurun. Akan tetapi beberapa dosis mampu meningkatkan kemampuan mutan dalam melarutkan fosfat dan kalium. Mutan  BPK5 pada dosis 7,5 kGy mampu melarutkan fosfat (165,67 ppm) dan kalium (18,89 ppm) yang lebih tinggi dibandingkan kontrol. Mutan FPF 4 pada dosis 2,5 kGy mampu melarutkan fosfat (418,15 ppm) lebih tinggi dibandingkan kontrol, sedangkan mutan FPF 4 mampu melarutkan kalium (13,90 ppm) lebih tinggi dibandingkan kontrol pada dosis 15 kGy. Perubahan pada tingkat molekuler diindikasikan dengan terjadinya perubahan basa pada sekuen DNA antara isolat induk (tanpa iradiasi) dengan sekuen mutan. Pada bakteri mutasi tertinggi terjadi pada transisi adenin menjadi guanin dan transversi timin menjadi sitosin dengan persentase masing-masing terhadap total perubahan sebesar 23,91 %. Perubahan basa pada sekuen DNA  isolat mutan fungi ditunjukkan dengan terjadinya  insersi adenin dan timin dengan persentase masing-masing terhadap perubahan total sebesar 50 %.
The effectiveness of application of phosphorous and potassium solubilizing multifunctional microbes (Aspergillus costaricaensis and Staphylococcus pasteuri mutants) on maize growth Desak Ketut Tristiana Sukmadewi; Iswandi Anas; Rahayu Widyastuti; Syaiful Anwar; Ania Citraresmini
Journal of Degraded and Mining Lands Management Vol 8, No 2 (2021)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2021.082.2681

Abstract

The use of phosphorus and potassium-solubilizing microbes as biofertilizers is an alternative method to increase the availability of phosphorus and potassium in soils. This study aimed to explore the effectiveness of phosphorus (P) and potassium (K)-solubilizing multifunctional microbes (Aspergillus costaricaensis and Staphylococcus pasteuri mutants) on maize growth. The stages of this study consisted of viability test of P and K solubilizing A. costaricaensis and S. pasteuri mutants in peat and effectiveness test of P and K solubilizing A. costaricaensis and S. pasteuri mutants on maize growth. The results showed that peat carriers could keep the fungi population stable until 18 weeks of storage times. While the bacteria at 6 and 8 weeks storage times showed a slight decrease and stable in the 10 to 12 weeks storage time. The addition of P and K-solubilizing multifunctional microbes could reduce the use of fertilizer up to 50% in the treatment with a combination of easily soluble P or K sources with not-easily soluble P or K sources, as well as a combination of treatments of not-easily soluble P and K sources. This tended to occur in the treatment with the addition of A. costaricaensis mutant. 
Effect Carrier Materials of Bradyrhizobium sp. strain PZS_A08 on Growth of Indigofera zollingeriana Wilhelmus Terang Arga Sanjaya; Sari Yulia Kartika; Desak Ketut Tristiana Sukmadewi; Rahayu Widyastuti; Iswandi Anas
JOURNAL OF TROPICAL SOILS Vol 26, No 2: May 2021
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2021.v26i2.95-103

Abstract

Sustainable forage production is needed to meet animal feed needs as the livestock industry increases. The purpose of this study is to evaluate the effect of liquid and solid carrier material on the effectiveness and infectivity of Bradyrhizobium sp. strain PZS_A08 on the growth of Indigofera zollingeriana (I. zollingeriana). The two carrier materials used were zeolite (solid) and molasses (liquid). This study used two carrier materials consisting of zeolite (solid) and molasses (liquid). The five treatments given were P0 (50% NPK), P1 (50% NPK+liquid biofertilizer), P2 (50% NPK+sterile liquid biofertilizer), P3 (50% NPK+solid biofertilizer), P4 (50 % NPK+sterile solid biofertilizer), P5 (100% NPK). Observations were made on the plant's height, number of leaves, number of nodules, upperparts and root wet biomass, root and upper parts dry biomass, root length, and microbial population). The use of liquid and solid carrier materials effectively affected the effectiveness and infectivity of Bradyrhizobium sp. strain PZS_A08 on  I. zollingeriana. Inoculants Bradyrhizobium sp. strain PZS_A08 significantly increased plants' growth and reduced the use of 50% NPK fertilizer. Cold storage temperature (5ºC) effectively maintained Bradyrhizobium sp. strain PZS_A08 on liquid and solid carriers, while solid carriers showed better effectiveness at room temperature storage (30oC). Through this research, solid carriers such as zeolite are recommended as carriers for Bradyrhizobium sp. filter PZS_A08.
The Effect of Gamma Irradiation on the Growth Rate of Phosphorus and Potassium Solubilizer Multifunctional Microbes Desak Ketut Tristiana Sukmadewi; Iswandi Anas; Ania Citraresmin
SEAS (Sustainable Environment Agricultural Science) Vol. 5 No. 1 (2021)
Publisher : Warmadewa University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.667 KB) | DOI: 10.22225/seas.5.1.3306.72-78

Abstract

Phosphorus (P) and potassium (K) solubilizing microbes have an important role in accelerating the solubility process of P and K sources which are difficult to dissolve. The purpose of this research was to study the effect of gamma irradiation on the growth rate of P and K solubilizer multifunctional microbes. The microbes used in this study were P and K solubilizer multifunctional bacteria (Staphylococcus pasteuri) and the P and K solubilizer multifunctional fungi (Aspergillus costaricaensis). The microbes were irradiated with gamma rays at a dose of 0; 1; 2.5 kGy. Tests for the rate of bacterial growth were observed through the results of optical density and Total Plate Count (TPC), while fungi were observed through measurement of colony diameter and TPC. Gamma irradiation effects increasing the growth rate of P and K solubilizer multifunctional bacteria (1.0 BPK 5 and 2.5 BPK 5) based on optical density values. At the time of calculating the population using TPC method, the increase in bacterial growth rate tended to be shown by isolates 2.5 BPK 5. The effect of gamma irradiation on the growth rate of fungi was indicated by the colony diameter of the isolate 1.0 BPK 5 which was wider than the wild type isolate or isolate 2.5 BPK 5. TPC results on fungi also showed that gamma irradiation affected the growth rate of fungi at 24, 96, 120, and 288 hours incubation time. Keyword: Gamma irradiation; microbe; growth and fertilizer
Implementation of LEISA Concept through composting rice straw waste in Subak Telun Ayah, Tegallalang Muliarta Muli I Nengah; Desak Ketut Tristiana Sukmadewi; Dewa Gede Wiryangga Selangga; I Gede Kariasa; Desak Ayu Diah Prawerti; I Komang Adi Parwata; I Wayan Landra
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol 7, No 4 (2022): November 2022
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v7i4.7825

Abstract

In general, many farmers tend to still regard rice straw as waste material that is burned. Burning rice straw is a bad habit because it removes organic matter that contains nutrients needed by plants. As a result, farmers require higher fertilizer costs in the next planting period which is contrary to the concept of Low External Input Sustainable Agriculture (LEISA). Community Service in Subak Telun Ayah aims to increase farmers' knowledge related to the use of compost in rice cultivation and as an effort to reduce inorganic fertilization. The methods used are surveys, interviews, discussions, counseling, and plot demonstrations. Farmers claim to burn rice straw because it is based on the knowledge passed down from generation to generation and it is believed that straw waste is quickly returned to the soil. Farmers do not do composting because they do not know how to compost rice straw. Based on farmers' problems, assistance is carried out by providing training and making demonstration plots. After the mentoring program, farmers are willing to do composting, moreover, it takes 30-35 days and is repeated every 7 days. Farmers' interest in composting is also triggered by the difficulty of obtaining inorganic fertilizers and the rising price of inorganic fertilizers. 
Development of Innovative Taro-Based Product Management with Zero-Waste Concept to Increase the Independence of PKK Wanagiri Luh Suriati; I Ketut Selamet; Desak Ketut Tristiana Sukmadewi
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 7 No. 1 (2023)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.182 KB) | DOI: 10.29165/ajarcde.v7i1.235

Abstract

The development of innovative products is currently growing rapidly producing a variety of processed products that are increasingly diverse. Taro tubers are one of the potential foodstuffs to be developed. Taro in addition to having carbohydrate component, also contains other components that are good for health. Taro has the disadvantage of itching when consuming taro due to the oxalate compounds contained in it. This has resulted in people being reluctant to process taro. This problem was experienced by one of the groups in Wanagiri Village, namely the Prosperous Family Empowerment Group (PKK) Wanagiri, which is located in Sukasada District, Buleleng Regency, Province Bali, Indonesia. The partner does not yet know the taste of taro, and does not have innovative product management made from taro. Solutions to overcome such problems are: providing knowledge regarding the cultivation and processing of taro, providing training on the processing of taro products (bread, nuggets and jams) and how to package, market and handle processing waste. The method used is active community participation. Community Self-Reliance Activities (KKM) in Wanagiri Village have been running smoothly. The added value of the Wanagiri PKK group and their standard of living has increased. Taro-based innovative product development with a zero-waste concept can be applied. Knowledge of how to process taro into bread, nuggets, chips and jam, product packaging, and marketing can help people to increase self-sufficiency. Mentoring activities should be carried out on an ongoing basis so that groups are able to independently make products (chips, bread, nuggets and jam), wider marketing and ultimately increased welfare.
Co-Authors Aisamrotul Hasanah Ania Cintaresmini Ania Citraresmin Ania Citraresmin Ania Citraresmini Ania Citraresmini Desak Ayu Diah Prawerti Dewa Gede Wiryangga Selangga DWI ANDREAS SANTOSA Fahrizal Hazra I Gede Kariasa I Ketut Selamet I Komang Adi Parwata I Wayan Landra Isna Arofatun Nikmah Iswandi Anas Iswandi Anas Iswandi Anas Iswandi Anas Iswandi Anas Iswandi Anas Jamhari Jamhari Luh Suriati Muliarta Muli I Nengah RAHAYU WIDYASTUTI Sari Yulia Kartika Sarjiya Antonius Sarjiya Antonius Suharjono Suharjono Syaiful Anwar Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya