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All Journal Jurnal Kimia Mulawarman BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Science Midwifery Lumbung Farmasi : Jurnal Ilmu Kefarmasian
ALIYAH FAHMI
UNIVERSITAS EFARINA
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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PENGARUH KANDUNGAN ASAM LEMAK BEBAS (ALB) TERHADAP PERUBAHAN DENSITAS CRUDE PALM OIL (CPO) ALIYAH FAHMI
JURNAL KIMIA MULAWARMAN Vol 17 No 2 (2020)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v17i2.622

Abstract

Density are a physical parameter of Crude Palm Oil (CPO) quality determination which done by gravimetric method (weighing sample in a picnometer 50 mL, g/mL). Density is affected by the temperature. High temperature in CPO may cause the density become less by a little. But if we get them in lower temperature will make them in solid form and this situation is undesirable in trade industry. High temperature may cause hydrolysis reaction that add free fatty acid (FFA) content. The density changing was made with variation temperature (450C, 500C, 550C, 600C dan 650C) and FFA concentration (2%,3%,4%,5% and 6%). The result in this research was high concentration of FFA will make CPO density decreased with inreasing temperature. The linier relationship happened in same temperature (450C), the CPO density with free fatty acid content were 2%, 3%, 4%, 5% and 6% were ?= 0,8934; 0,8391; 0,8926; 0,8921 and 0,8920), so did the temperature at 500C, 550C, 600C dan 650C Keywords: Density, CPO, FFA, Temperature
The Antibacterial Activity Test Comparison of Green and Black Tea Ethanol Extract (Camellia sinensis) Against Propionibacterium acnes Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
Science Midwifery Vol 10 No 2 (2022): April: Science Midwifery
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v10i2.544

Abstract

A study was conducted in connection with the comparison of antibacterial activity tests between ethanol extracts from green tea leaves and black tea leaves (Camellia sinensis) against Propionibacterium acnes. There were obtained by a softening technique that was softened for 24 hours. The filtrate was then concentrated to give a concentrated extract and evaporated ethanol content. The concentrated extract was then diluted with distilled water to extract 5%, 10%, and 15% concentration variations. The test method for inhibitory activity was the disc method using distilled water as a negative blank. The results obtained with blank, 5%, 10% and 15% green tea were 0. 5.25; 7.05 and 7.95 mm and black tea ethanol extract were 0; 2.50; 3.20 and 5.30 mm. The conclusion of this study is that the concentration of the extract is directly proportional to its inhibitory power, and the green tea ethanol extract is more potent than the black tea ethanol extract, so both can be used as antibacterial agents against P. acnes.
GREEN TEA ETHANOL EXTRACT EFFICACY AGAINST PSEUDOMONAS AERUGINOSA Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 9, No 1 (2022): August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i1.7336

Abstract

Research about green tea ethanol extract efficacy against Pseudomonas aeruginosa has been done. The green tea leaves were sourced from tea distributor in Sidamanik District, North Sumatra, Indonesia. Green tea leaves extract was obtained by maceration technique in which green tea leaves were soaked with ethanol 96% for 24 hours. After that, the filtrate was concentrated to be thick extract with no liquid content again.  After that, the concentrated extract was made by 5%, 10% and 15% as extract variations. The efficacy of green tea ethanol extract against Pseudomonas aeruginosa  was signed by increasing the inhibitory diameter that using disc method, in which the distilled water was used as negative blank. The results obtained for blanks, 5%, 10% and 15% extracts were 0; 1.85; 2.9 and 4.45 mm. The conclusion of this study is that the concentration of the extract is increasing proportionally to its inhibitory power, the higher of extract concentration is higher of its inhibitory activity against Pseudomonas aeruginosa. The conclusion described the green tea ethanol extract was effective to be antibacterial agent against Pseudomonas aeruginosa.
Isolasi dan Karakterisasi Bakteri Asam Laktat dari Teh Hijau Fermentasi Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
Lumbung Farmasi: Jurnal Ilmu Kefarmasian Vol 3, No 2 (2022): Juli
Publisher : UNIVERSITAS MUHAMMADIYAH MATARAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/lf.v3i2.9645

Abstract

ABSTRAKTeh hijau adalah jenis teh yang tidak mengalami proses fermentasi akan tetapi mengalami proses pengeringan dan penguapan daun. Teh hijau memiliki aktivitas antioksidan yang tinggi karena kandungan katekin di dalamnya. Namun bioavaibilitas katekin teh hijau berkurang pada saluran cerna karena kurang stabil. Kehadiran probiotik pada teh hijau diharapkan mampu meningkatkan bioavaibilitasnya. Bakteri Asam Laktat (BAL) merupakan kelompok bakteri baik yang merupakan probiotik yang berperan dalam menyehatkan saluran cerna. Peneltian ini bertujuan untuk mengisolasi dan menguji karakterisasi BAL dari Teh Hijau Fermentasi. Teh hijau yang berasal dari Distributor Teh Juma, Kecamatan Sidamanik difermentasi dengan gula aren dan madu hutan dengan variasi waktu 24, 48 dan 72 jam pada suhu rendah. Teh hijau fermentasi kemudian diisolasi kemudian diencerkan dengan pengenceran 10-5 dan 10-6. Hasil inkubasi selama 48 jam diperoleh kemudian dimurnikan dengan metode streak dan diinkubasi kembali selama 24 jam kemudian diuji karakterisasinya secara morfologi, pewarnaan gram, uji katalase dan uji fermentasi. Hasil yang diperoleh adalah pada uji morfologi positif BAL dengan ciri-ciri terlihat koloni tunggal dengan bentuk bulat, licin, warna putih kekuningan.dan terbentuk zona bening di sekitar koloni. Hasil pewarnaan positif BAL dengan ciri-ciri bakteri gram positif ditunjukkkan oleh warna biru ke ungu. Hasil uji katalase ditunjukkan dengan ciri-ciri  tidak menghasilkan gelembung udara yang menandakan adanya BAL karena BAL tidak mengandung oksigen serta uji fermentasi yang ditunjukkan dengan tidak terbentuknya gelembung udara pada tabung Durham yang menandakan tipe fermentasi BAL adalah homofermentatif. Kata kunci : Isolasi; Karakterisasi; Bakteri asam laktat; Teh hijau; Fermentasi.ABSTRACTGreen tea is a type of tea that does not undergo a fermentation process but undergoes a process of drying and evaporation of the leaves. Green tea has high antioxidant activity due to the catechin content in it. However, the bioavailability of green tea catechins is reduced in the gastrointestinal tract because it is less stable. The presence of probiotics in green tea is expected to increase its bioavailability. Lactic Acid Bacteria (LAB) is a group of good bacteria which are probiotics that play a role in healthy gastrointestinal tract. This study aims to isolate and test the characterization of LAB from Fermented Green Tea. Green tea from Juma Tea Distributor, Sidamanik District was fermented with palm sugar and forest honey with variations of 24, 48 and 72 hours at low temperature. The fermented green tea was isolated and then diluted with 10-5 and 10-6 dilutions. The results of incubation for 48 hours were obtained and then purified by streak method and incubated again for 24 hours and then tested for morphological characterization, gram staining, catalase test and fermentation test. The results obtained were a positive morphological test of LAB with the characteristics of a single colony being seen with a round, smooth, yellowish white color, and a clear zone was formed around the colony. The results of positive LAB staining with gram-positive bacteria are indicated by a blue to purple color. The results of the catalase test are indicated by the characteristics of not producing air bubbles which indicate the presence of LAB because LAB does not contain oxygen and the fermentation test is indicated by the absence of air bubbles in the Durham tube which indicates the type of LAB fermentation is homofermentative. Keywords : Isolation; Characterization; Lactic acid bacteria; Green tea; Fermentation;
GREEN TEA ETHANOL EXTRACT EFFICACY AGAINST PSEUDOMONAS AERUGINOSA Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 9 No. 1 (2022): August 2022
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i1.7336

Abstract

Research about green tea ethanol extract efficacy against Pseudomonas aeruginosa has been done. The green tea leaves were sourced from tea distributor in Sidamanik District, North Sumatra, Indonesia. Green tea leaves extract was obtained by maceration technique in which green tea leaves were soaked with ethanol 96% for 24 hours. After that, the filtrate was concentrated to be thick extract with no liquid content again.  After that, the concentrated extract was made by 5%, 10% and 15% as extract variations. The efficacy of green tea ethanol extract against Pseudomonas aeruginosa  was signed by increasing the inhibitory diameter that using disc method, in which the distilled water was used as negative blank. The results obtained for blanks, 5%, 10% and 15% extracts were 0; 1.85; 2.9 and 4.45 mm. The conclusion of this study is that the concentration of the extract is increasing proportionally to its inhibitory power, the higher of extract concentration is higher of its inhibitory activity against Pseudomonas aeruginosa. The conclusion described the green tea ethanol extract was effective to be antibacterial agent against Pseudomonas aeruginosa.
Co-Authors Melia, Sri Sumaryati Syukur Zulkarnain Chaidir