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All Journal Indonesian Journal of Biotechnology Majalah Obat Tradisional
Kazutaka maeyama
Department of Pharmacology, Informational Biomedicine, School of Medicine, Ehime University Japan
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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EFEK SENYAWA FLAVONOIDS DARI KEMUNING (Murraya paniculata [L.] Jack.) TERHADAP PELEPASAN HISTAMIN DARI KULTUR SEL MAST Nugroho, Agung Endro; Riyanto, Sugeng; Sukari, Mohamad Aspollah; Maeyama, Kazutaka
Majalah Obat Tradisional Vol 15, No 1 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (503.985 KB) | DOI: 10.14499/mot-TradMedJ15iss1pp%p

Abstract

Murraya paniculata [L.] Jack. merupakan tanaman yang tumbuh di Indonesia, dikenal dengan nama Kemuning. Penelitian mengenai tanaman ini telah banyak dilakukan, terutama isolasi senyawa aktifnya. Tanaman ini mempunyai kandungan senyawa aktif, diantaranya senyawa turunan flavonoid. Pada penelitian, tiga senyawa flavonoid yang diisolasi dari M. paniculata diuji aktivitasnya terhadap pelepasan histamin dari kultur sel mast yaitu sel RBL-2H3. Ketiga senyawa tersebut adalah 3,3’,4’,5,5’,7–heksametoksiflavon; 3,3’,4’,5,5’,6,7-heptametoksiflavon; dan 3,3’,4’,5,5’,6,7,8-oktametoksiflavon. Induktor pelepasan histamin yang digunakan adalah DNP24-BSA dan thapsigargin. Keduanya berturut-turut menginduksi secara imunologis dan non-imunologis. Hasil penelitian menunjukkan bahwa senyawa heptametoksiflavon dan heksametoksiflavon cenderung tidak mempengaruhi pelepasan histamin dari sel mast. Namun, oktametoksiflavon dapat meningkatkan pelepasan histamin dari sel mast baik tanpa induksi maupun terinduksi dengan DNP24-BSA atau thapsigargin. Senyawa tersebut mampun meningkatkan pelepasan histamin hingga 50%. Dari hasil tersebut, penambahan gugus polimetoksi pada struktur flavonoid berpotensi dapat menghasilkan efek pelepasan histamin dari sel mast.  
Comparison of Cytotoxic and Antiproliferative Effects of Benzylidenecyclopentanone Analogues of Curcumin on RBL-2H3 Cells Nugroho, Agung Endro; ., Sardjiman; Maeyama, Kazutaka
Indonesian Journal of Biotechnology Vol 15, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.862 KB)

Abstract

Curcumin is a natural yellow pigment isolated from the rhizomes of Curcuma longa L. (turmeric), and has several pharmacological effects and no toxicity in both in animal and human clinical study. However, the problem of curcumin is its stability because of its active methylene moiety. Modification of this moiety to cyclopentanone is expected to increase the stability. Previous study reported that benzylidenecyclopentanone analogues of curcumin showed inhibitory effect on histamine release from RBL-2H3 (rat basophilic leukemia) cells, a tumor analog of mast cells. One of them, the hydroxy-methoxy analog (PGV-0), showed more potent effect than that of curcumin. In the present study, some benzylidenecyclopentanone analogues of curcumin were evaluated for their effects on the viability and proliferation of RBL-2H3 cells. Viable cells were counted under a light microscope with a cells-counting chamber or using the cell viability reagent WST-1. The results showed that mast cell viability and histamine content were not affected by curcumin and benzylidene cyclopentanone for 30 min incubation, however, impaired for overnight incubation. The hydroxy-dimethyl benzylidene analog (PGV-1) strongly decreased the mast cells viability for overnight incubation, and its effect was highest among the other analogues. In the proliferation study, this compound also strongly inhibited the proliferation of mast cells, whereas curcumin and hydroxy-methoxy benzylidene analog inhibited the proliferation slightly. There were no inhibitory effects on mast cells proliferation treated by dibenzylidene; dihydroxybenzylidene; and hydroxy-diethylbenzylidene cyclopentanone.Keywords : viability, proliferation, curcumin, benzylidene cyclopentanone, RBL-2H3 cells
Co-Authors Agung Endro Nugroho Mohamad Aspollah Sukari, Mohamad Aspollah Sardjiman . Sugeng Riyanto