Garuda - Garba Rujukan Digital

PRODUKSI KITOSAN SECARA ENZIMATIK OLEH Bacillus firmus E65 UNTUK PENGENDALIAN PENYAKIT ANTRAKNOSA PADA BUAH MANGGA (Mangifera Indica L.) Dwina Mulyaningtyas; Susiana Purwantisari; Endang Kusdiyantini; Yadi Suryadi
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.196 KB)

Anthracnose is postharvest disease on mango caused by fungi Colletotrichum gloesporioides. Anthracnose can be inhibited development by chitosan as a preservative anti-fungal for fruit. Effectiveness of chitosan can be increased by enzymatic hydrolyze method to obtain a low molecular weight chitosan. Low molecular weight chitosan can be reducing the particle size into nano chitosan. This study aims to obtain the low molecular weight of chitosan by hydrolyzing the commercial chitosan enzymatically using B. firmus E65, the production of nano chitosan with ionic gelation method and to determine the volume ratio of low molecular weight of chitosan and natrium tripolifosfat (NaTPP) to conduct in vitro and in vivo inhibition on the growth of fungi C. gloeosporioides. The method consist of the production of chitinase from B. firmus E65, the production chitinase of low molecular weight chitosan, ionic gelation, in vitro and in vivo nanochitosan bioassay test on the growth of C. gloeosporioides. The research result showed that the activity of the crude extract of chitinase is 0.05 U/mL and the purity is 0.07 U/mL. The spesific activity value of crude extract is 0.44 U/mg, and the purity is 0.82 U/mg. The purity level of crude extract is increased 0.83 times from 1 to 1.83 times after purification process. Molecular weight of enzimatically hydrolyzed chitosan is 511.850 Kda. In vitro and in vivo bioassay showed the best result on the growth of C. gloeosporioides are 90 and 91% is obtained in the combination 3:1 of the low molecular weight chitosan and NaTTP. This comparison showed that the size of nano chitosan is 228.74 and the polidispesity index is 0.884. Keywords : Chitinase, chitosan, B, firmus E65, antrachnose, C. gloesporioides.

PRODUKSI ENZIM INULINASE Pichia manshurica DUCC-Y015 DENGAN PENAMBAHAN SUBSTRAT TEPUNG BENGKOANG (Paschyrhizus erosus) Adzar Rofiqoh; W Wijanarka; Susiana Purwantisari
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.657 KB)

Bengkoang (Pachyrhizus erosus) tubers has a high inulin content. Inulin bengkoang flour can be used as substrate to produce inulinase enzyme. The inulinase enzyme can be produced by Pichia manshurica DUCC-Y015. This research aims to determine the ability of Pichia mansurica DUCC-Y015 in producing inulinase enzyme with the addition of several variations of substrate concentration of bengkoang flour in its production medium. Determination of inulinase activity was done by DNS method. This research used a Completely Randomized Design (RAL) with 4 treatments: B0 (control), B1 (1 g bengkoang flour), B2 (3 g bengkoang flour) and B3 (5 g bengkoang flour). Each treatment was repeated 3 times. The inulinase activity of each treatment was 0.029 IU/mL, 0.033 IU/mL, 0.053 IU/mL and 0.015 IU/mL. The addition of variation substrate concentration bengkoang flour in the production medium did not affect the inulinase activity of Pichia manshurica DUCC-Y015Kata Kunci: Pachyrhizus erosus, inulinase, Pichia manshurica DUCC-Y015.

HUBUNGAN KUALITAS MIKROBIOLOGI AIR BAKU DAN HIGIENE SANITASI DENGAN CEMARAN MIKROBA PADA AIR MINUM ISI ULANG DI KECAMATAN TEMBALANG Endang Sri Utami; Martini Martini; Lintang Dian Saraswati; Susiana Purwantisari
Jurnal Kesehatan Masyarakat (Undip) Vol 6, No 1 (2018): JANUARI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Based on Indonesian Health Ministry regulation No. 492/ MENKES/PER/ IV/2010, the MPN coliform dan E.coli of drinking water should be 0 of 100 mL sample. A previous research showed that 95% of samples did not require the microbiological contamination. The research aimed to analyze the correlation between microbiological quality of raw water and hygiene sanitation with microbiological contamination of refilled drinking water. The type of this research is analytic with cross sectional approach. The samples are 47 depots and 55 workers. The results showed that 85.1% of samples did not require the microbiological contamination. Using by Chi-square test analysis (α=5%) indicates that the personal hygiene has p=0.035 (OR=10,000 and CI=1.095 to 91.309). It means personal hygiene have a correlations with microbiological contamination of refilled drinking water. While the microbiological quality of the raw water (p=0.188), the conditions of depots sanitary (p=0.999), the conditions of equipment sanitary (p=0.410) were not associated with the contamination of refilled drinking water. Therefore, it is necessary to optimize the sanitary inspection and testing the sampels frequently. Also, socialization for depot’s workers is needed to increase the hygiene sanitation.

HUBUNGAN HIGIENE PENJAMAH DENGAN KUALITAS MIKROBIOLOGIS PADA MINUMAN ES COKLAT DI KOTA SEMARANG (Studi di Kecamatan Tembalang Dan Kecamatan Pedurungan) Dwi Rahayuningsih; Martini Martini; Susiana Purwantisari; Retno Hestiningsih
Jurnal Kesehatan Masyarakat (Undip) Vol 5, No 4 (2017): JULI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Water borne disease is a disease caused by the entry of pathogenic bacteria in the body with drinks. Ice chocolate currently many outstanding in the community. Tembalang and Pedurungan, Semarang are an educational area so it is a strategic place to sell ice chocolate. The purpose of this research is to analyze relations between hygiene sanitation with microbiological quality in ice chocolate in Semarang (Study in Tembalang and Pedurungan). This study is observational-analytic with using cross sectional design. The samples were 38 samples of ice chocolate. Data were analyzed using Chi square. The results showed that most of the ice chocolate did not qualify with 35 samples (92,1%) of unqualified Coliform status, 21 samples (55,3%) were contaminated with Escherichia coli, and 6 samples (15,7%) were contaminated with yeast / mold / fungi. This study shows there is an relations between hygiene handlers with microbiological quality in ice chocolate (p value = 0,043). Traders should pay attention to hygiene when making ice chocolate so that bacterial contamination can be minimized.

GAMBARAN SANITASI TEMPAT BERDAGANG WARUNG PENYET DI KECAMATAN TEMBALANG Barkah Haryo Wasisto; Martini Martini; Sri Yuliawati; Susiana Purwantisari
Jurnal Kesehatan Masyarakat (Undip) Vol 5, No 1 (2017): JANUARI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

District Tembalang is one of the sub-district in the city of Semarang with many colleges so that there are many penyet restaurant. The purpose of this study was to determine the factors that influence the contamination of Salmonella sp on a vegetables penyet in District Tembalang, Semarang City. This study is observational analytical survey method using cross sectional design. These samples included 40 penyet restaurant observed water and vegetables fresh vegetables supplied to consumers. Data were analyzed by frequency distribution and bivariate with chi square test. The results showed the storage of vegetables vegetables using plastic bags (50%); the basket (27.5%) and sacks (22.5%). Sanitation trade places category is good (57.5%). There is a statistically significant relationship between sanitation trade places (p = 0.006) with Salmonella sp contamination in fresh vegetables. Traders are paying less attention sanitation and personal hygiene as well as a sanitation vegetables can contaminate vegetables in the vegetable vegetables Salmonella sp. Penyet restaurant traders should begin to attention about hygiene and sanitation where trade, personal hygiene when to trade, and improve the way the vegetable washing vegetables to reduce the risk of contamination of Salmonella sp.

Desa Sukodono adalah salah satu wilayah di Kabupaten Demak yang endemik filariasis (angka rata-rata 1,39%). Penemuan kasus baru dan mikrofilaria pada tubuh penderita kronis meningkatkan potensi terjadinya penularan dan infeksi mikrofilaria pada nyamuk. Pe Retno Hestiningsih; Elsye Giovanny Puspitasari; Martini Martini; Atik Mawarni; Susiana Purwantisari
Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Vol 9 No 2 (2019): April
Publisher : LPPM STIKES KENDAL

Desa Sukodono adalah salah satu wilayah di Kabupaten Demak yang endemik filariasis (angka rata-rata 1,39%). Penemuan kasus baru dan mikrofilaria pada tubuh penderita kronis meningkatkan potensi terjadinya penularan dan infeksi mikrofilaria pada nyamuk. Penelitian ini bertujuan untuk mengetahui populasi Culex sp sebagai vektor filariasis. Penelitian ini adalah survei deskriptif dengan desain cross sectional. Sampel pada 155 rumah tangga. Nyamuk dikumpulkan menggunakan metode pengumpulan pendaratan dan pengumpulan istirahat pada pukul 6 sore - 6 pagi. Pengamatan lingkungan dilakukan untuk mengidentifikasi kondisi lingkungan (tempat berkembang biak dan tempat peristirahatan) Culex sp. Hasil penelitian menunjukkan bahwa spesies Culex ditangkap di desa Sukodono termasuk Cx. quinquefasciatus (58,5%), Cx. vishnui (35,8%), Cx. tritaeniorhychus (4,2%). Tingkat infeksi 0%. Sedangkan dari parsial Cx quinquefasciatus 12%, Cx. vishnui 13%, dan Cx. tritaeniorhychus 36%. Rata-rata suhu dan kelembaban 33.190C dan 67,93%. Ada tempat berkembang biak dan tempat istirahat berupa semak (69,7%), rawa (83,2%), beras (100%), genangan air (53,3%) dan ternak (unggas (93,5%); kambing (11%)) yang mendukung kepadatan nyamuk. Kepadatan tertinggi dari larva ditemukan di parit (1,4 larva). Proses penularan filariasis di Desa Sukodono masih terjadi, sehingga perlu kewaspadaan dan peningkatan upaya pencegahan filariasis. Bagi masyarakat, kebutuhan untuk meningkatkan upaya pengendalian vektor dan perbaikan lingkungan. Kata kunci: Filariasis, Culex, Angka Infeksi, Parousitas POPULATION OF CULEX SP AS VECTORS OF FILARIASIS ABSTRACT Sukodono village is one of area in Demak district witch was endemic of filariasis (mf rate 1,39%). The discovery of new cases and microfilaria on chronic sufferers’ body raises the potential occurrence of transmission and infection microfilaria on mosquitoes. This research aims to know population of Culex sp as vectors of filariasis. This study was an descriptive survey by cross sectional design. The sample was on 155 households. Mosquito was collected using a method of landing collection and resting collection at 6 pm - 6 am. Environmental observations performed to identify environmental conditions (breeding places and resting places) of Culex sp. The results showed that the species of Culex were caught in Sukodono village including Cx. quinquefasciatus (58,5%), Cx. vishnui (35,8%), Cx. tritaeniorhychus (4,2%). Infection rate 0%. While of paroucity of Cx quinquefasciatus 12%, Cx. vishnui 13%, and Cx. tritaeniorhychus 36%. The average of temperature and humidity 33,190C and 67,93%. There are breeding places and resting places in the form of shrubs (69.7%), swamp (83.2%), rice (100%), puddle (53.3%) and livestock (poultry (93.5%); goat (11%)) that support mosquito density. The highest density of larvae found in ditch (1,4 larvas). The process of transmission of filariasis in Sukodono village was still happened, so that it takes vigilance and improvement of filariasis prevention efforts. For the community, the need to enhance the efforts of vector control and environmental improvement. Keywords: Filariasis, Culex, Infection Rate, Paroucity

PUPUK BERBAHAN AKTIF Trichoderma spp. SEBAGAI AGEN HAYATI TERHADAP PERTUMBUHAN TANAMAN KENTANG DI DESA KAPONAN KECAMATAN PAKIS, MAGELANG Harum Sitepu Sitepu; Susiana Purwantisari; Ryantoko Ryantoko
Agros Journal of Agriculture Science Vol 25, No 1 (2023): edisi JANUARI
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar

Trichoderma spp. adalah jamur antagonis yang secara alami merupakan parasit dan mampu menyerang banyak jenis jamur penyebab penyakit tanaman sehingga berpotensi digunakan sebagai pupuk yang mampu dimanfaatkan dalam upaya pengendalian hayati sekaligus alternatif pengganti pupuk kimia. Penelitian ini bertujuan untuk mengetahui dosis dan cara pemberian pupuk Trichoderma spp. yang efektif digunakan untuk memacu pertumbuhan kentang, dan yang menunda serangan penyakit pada tanaman kentang. Penelitian ini dilakukan menggunakan metode eksperimental dengan menggunakan metode Rancangan Acak Lengkap (RAL)dengan 5 (lima) perlakuan yang terdiri dari atas 5 perlakuan dan kontrol yaitu P0 :tanpa perlakuan Trichoderma; P1 :aplikasi semprot Trichoderma 80 gram/10 liter air. P2 :aplikasi semprot Trichoderma 100 gram/10 liter air, P3 :aplikasi Kocor Trichoderma 80 gram/10 liter air,;P4 : aplikasi Kocor Trichoderma 100 gram/10 liter air,; P5: aplikasi Kocor + Semprot Trichoderma 80 gr/10 liter air. Penelitian ini dilaksanakan di Desa Kaponan, Kecamatan Pakis, Magelang dengan ketinggian 1.200 m dari permukaan laut. Hasil penelitian menunjukkan pengukuran tinggi tanaman perlakuan P5 secara signifikan berbeda secara dengan P0 (kontrol) sedangkan perlakuan lainnya (P1,P2,P3,dan P4) tidak terlau berbeda signifikan. Pengukuran jumlah daun, perlakuan P1 dan P5 berbeda secara signikan dengan P0 (kontrol), sedangkan perlakuan lainnya (P2,P3, dan P4) tidak terlalu berbeda signifikan. Pengukuran banyaknya pertumbuhan tunas setiap tanaman, perlakuan P1 dan P5, berbeda secara signifikan dengan P0 (kontrol), sedangkan perlakuan lainnya (P2,P3, dan P4) tidak terlalu berbeda signifikan. Untuk pengukuran skore serangan penyakit P1,P4, dan P5 berbeda secara signikan dengan P0 (kontrol), sedangkan perlakuan lainnya (P2, dan P3) tidak terlalu berbeda signifikan.Pengukuran produksi umbi kentang per-tanaman perlakuan P1 dan P5, berbeda secara signifikan dengan P0 (kontrol), sedangkan perlakuan lainnya (P2,P3, dan P4) tidak terlalu berbeda signifikan. Kesimpulan dari penelitian adalah aplikasi Kocor + Semprot Trichoderma dan dosis pupuk Trichoderma spp. 80gram/10 liter air yang paling baik meningkatkan pertumbuhan dan menunda serangan penyakit pada tanaman kentang.

PUPUK BERBAHAN AKTIF Trichoderma spp. SEBAGAI AGEN HAYATI TERHADAP PERTUMBUHAN TANAMAN KENTANG DI DESA KAPONAN KECAMATAN PAKIS, MAGELANG Harum Sitepu; Susiana Purwantisari; Ryantoko Setyo Prayitno
Agros Journal of Agriculture Science Vol 25, No 1 (2023): edisi JANUARI
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v25i1.2386

Trichoderma spp. adalah jamur antagonis yang secara alami merupakan parasit dan mampu menyerang banyak jenis jamur penyebab penyakit tanaman sehingga berpotensi digunakan sebagai pupuk yang mampu dimanfaatkan dalam upaya pengendalian hayati sekaligus alternatif pengganti pupuk kimia. Penelitian ini bertujuan untuk mengetahui dosis dan cara pemberian pupuk Trichoderma spp. yang efektif digunakan untuk memacu pertumbuhan kentang, dan yang menunda serangan penyakit pada tanaman kentang. Penelitian ini dilakukan menggunakan metode eksperimental dengan menggunakan metode Rancangan Acak Lengkap (RAL)dengan 5 (lima) perlakuan yang terdiri dari atas 5 perlakuan dan kontrol yaitu P0 :tanpa perlakuan Trichoderma; P1 :aplikasi semprot Trichoderma 80 gram/10 liter air. P2 :aplikasi semprot Trichoderma 100 gram/10 liter air, P3 :aplikasi Kocor Trichoderma 80 gram/10 liter air,;P4 : aplikasi Kocor Trichoderma 100 gram/10 liter air,; P5: aplikasi Kocor + Semprot Trichoderma 80 gr/10 liter air. Penelitian ini dilaksanakan di Desa Kaponan, Kecamatan Pakis, Magelang dengan ketinggian 1.200 m dari permukaan laut. Hasil penelitian menunjukkan pengukuran tinggi tanaman perlakuan P5 secara signifikan berbeda secara dengan P0 (kontrol) sedangkan perlakuan lainnya (P1,P2,P3,dan P4) tidak terlau berbeda signifikan. Pengukuran jumlah daun, perlakuan P1 dan P5 berbeda secara signikan dengan P0 (kontrol), sedangkan perlakuan lainnya (P2,P3, dan P4) tidak terlalu berbeda signifikan. Pengukuran banyaknya pertumbuhan tunas setiap tanaman, perlakuan P1 dan P5, berbeda secara signifikan dengan P0 (kontrol), sedangkan perlakuan lainnya (P2,P3, dan P4) tidak terlalu berbeda signifikan. Untuk pengukuran skore serangan penyakit P1,P4, dan P5 berbeda secara signikan dengan P0 (kontrol), sedangkan perlakuan lainnya (P2, dan P3) tidak terlalu berbeda signifikan.Pengukuran produksi umbi kentang per-tanaman perlakuan P1 dan P5, berbeda secara signifikan dengan P0 (kontrol), sedangkan perlakuan lainnya (P2,P3, dan P4) tidak terlalu berbeda signifikan. Kesimpulan dari penelitian adalah aplikasi Kocor + Semprot Trichoderma dan dosis pupuk Trichoderma spp. 80gram/10 liter air yang paling baik meningkatkan pertumbuhan dan menunda serangan penyakit pada tanaman kentang

Potential Zoonotic Faecal Bacteria from Sunda Porcupine (Hystrix javanica) and Their Antimicrobial Resistance Profiles Sarsa Nisa; Rifka A. Safitri; Nurul Inayah; Achirul Nditasari; Susiana Purwantisari; Rejeki Ferniah; Anang Achmadi; Taufiq Nugraha; Sugiyono Saputra
Microbiology Indonesia Vol. 15 No. 2 (2021): June 2021
Publisher : Indonesian Society for microbiology

Sunda porcupine (Hystrix javanica) is one of the Indonesian endemic species which is often sought after for their meat. Although it is becoming increasingly popular for extreme culinary, information regarding biological risks arising from this wildlife is very limited. This study aimed to assess potential zoonotic faecal bacteria carried by Sunda porcupine with culture-dependant approach and to investigate whether antimicrobial resistant isolates can be found in wildlife. A total 22 faecal samples were collected from captive Sunda porcupine and tested for the presence of pathogens in selective media for Salmonella and Listeria. After inoculating the samples in Rappaport-Vassiliadis (RV) Salmonella enrichment broth, two samples (9%) were regarded as positive for Salmonella in this presumptive test which indicated by growth black colonies on xylose lysine deoxycholate (XLD) agar. Meanwhile, the presence for Listeria was presumptively positive in all samples (100%), indicated by black colour appearance in Listeria isolation transwab. In total, 38 bacterial isolates were successfully purified, preserved and subjected for antimicrobial susceptibility testing (AST) by disk diffusion method. Resistance to ceftriaxone (3rd generation cephalosporins) was not detected while resistance to one or two antimicrobials was observed in seven isolates. Further, 16S rRNA bacterial identification was performed for selected isolates and based on sequence similarity on GenBankÂ® databases and phylogenetic tree construction, those isolates were denoted as Pseudomonas xinjiangensis (XG4.4), Shigella sonnei (XD8.2 and G11.3), Proteus mirabilis (XH3.3, H4.2, and E1.2) and Klebsiella quasipneumoniae subsp. similipneumoniae (XF4.2). All identified isolates were sensitive to amikacin, amoxicillin-clavulanic acid, cefoxitin and ceftriaxone, except for one isolate Shigella sonnei (XD8.2) which was resistant to cefoxitin. Further research to confirm the pathogenicity of the isolates is still needed but based on these results, we support the hypothesies that Sunda porcupine is potential as a reservoir pathogenic bacteria and preventive measures are crucial to prevent transmission when processing this bushmeat.

Title

Found 9 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 9 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 9 Documents
Search