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All Journal Jurnal Akademika Biologi Jurnal Kesehatan Masyarakat Microbiology Indonesia Jurnal Pertanian Agros Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal
Susiana Purwantisari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : Jurnal Akademika Biologi

 1 
PRODUKSI KITOSAN SECARA ENZIMATIK OLEH Bacillus firmus E65 UNTUK PENGENDALIAN PENYAKIT ANTRAKNOSA PADA BUAH MANGGA (Mangifera Indica L.) Dwina Mulyaningtyas; Susiana Purwantisari; Endang Kusdiyantini; Yadi Suryadi
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Anthracnose is postharvest disease on mango caused by fungi Colletotrichum gloesporioides. Anthracnose can be inhibited development by chitosan as a preservative anti-fungal for fruit. Effectiveness of chitosan can be increased by enzymatic hydrolyze method to obtain a low molecular weight chitosan. Low molecular weight chitosan can be reducing the particle size into nano chitosan. This study aims to obtain the low molecular weight of chitosan by hydrolyzing the commercial chitosan enzymatically using B. firmus E65, the production of nano chitosan with ionic gelation method and to determine the volume ratio of low molecular weight of chitosan and natrium tripolifosfat (NaTPP) to conduct in vitro and in vivo inhibition on the growth of fungi C. gloeosporioides. The method consist of the production of chitinase from B. firmus E65, the production chitinase of low molecular weight chitosan, ionic gelation, in vitro and in vivo nanochitosan bioassay test on the growth of C. gloeosporioides. The research result showed that the activity of  the crude extract of chitinase is 0.05 U/mL and the purity is 0.07 U/mL. The spesific activity value of crude extract is 0.44 U/mg, and the purity is 0.82 U/mg. The purity level of crude extract is increased 0.83 times from 1 to 1.83 times after purification process. Molecular weight of enzimatically hydrolyzed chitosan is  511.850 Kda. In vitro and in vivo bioassay showed the best result on the growth of C. gloeosporioides are 90 and 91% is obtained in the combination 3:1 of the low molecular weight chitosan and NaTTP. This comparison showed that the size of nano chitosan is 228.74 and the polidispesity index is 0.884. Keywords : Chitinase, chitosan, B, firmus E65, antrachnose, C. gloesporioides.
PRODUKSI ENZIM INULINASE Pichia manshurica DUCC-Y015 DENGAN PENAMBAHAN SUBSTRAT TEPUNG BENGKOANG (Paschyrhizus erosus) Adzar Rofiqoh; W Wijanarka; Susiana Purwantisari
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bengkoang (Pachyrhizus erosus) tubers has a high inulin content. Inulin bengkoang flour can be used as substrate to produce inulinase enzyme. The inulinase enzyme can be produced by Pichia manshurica DUCC-Y015. This research aims to determine the ability of Pichia mansurica DUCC-Y015 in producing inulinase enzyme with the addition of several variations of substrate concentration of bengkoang flour in its production medium. Determination of inulinase activity was done by DNS method. This research used a Completely Randomized Design (RAL) with 4 treatments: B0 (control), B1 (1 g bengkoang flour), B2 (3 g bengkoang flour) and B3 (5 g bengkoang flour). Each treatment was repeated 3 times. The inulinase activity of each treatment was 0.029 IU/mL, 0.033 IU/mL, 0.053 IU/mL and 0.015 IU/mL. The addition of variation substrate concentration bengkoang flour in the production medium did not affect the inulinase activity of Pichia manshurica DUCC-Y015Kata Kunci: Pachyrhizus erosus, inulinase, Pichia manshurica DUCC-Y015.
Co-Authors Achirul Nditasari Adzar Rofiqoh Anak Agung Istri Sri Wiadnyani Anang Achmadi Atik Mawarni Barkah Haryo Wasisto Dwi Rahayuningsih Dwina Mulyaningtyas Elsye Giovanny Puspitasari Endang Kusdiyantini Endang Sri Utami Harum Sitepu Harum Sitepu Sitepu Lintang Dian Saraswati Martini Martini Nurul Inayah Rejeki Ferniah Retno Hestiningsih Rifka A. Safitri Ryantoko Setyo Prayitno Sarsa Nisa Sri Yuliawati Sugiyono Saputra Taufiq Nugraha w wijanarka Yadi Suryadi