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Agung Suprihadi
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UJI AKTIVITAS KITIN DEASETILASE ISOLAT BAKTERI DARI KAWASAN GEOTERMAL DIENG Ghaida Afra Akhsani; Agung Suprihadi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Chitinolytic bacteria is a bacterium, which is able to degrade chitin. This ability is obtained from the resulted chitinolytic enzyme. Chitin deacetylase (EC 3.5.1.41) is one of the chitinolytic enzymes, which be able to convert chitin into its derivatives. For this reason, chitin deacetylase has a chance to be an environmentally enzymatic converter of chitin. In addition, chitin derivatives have a wider potential in many fields. The objectives of this study were to obtain bacterial isolates from the mud of Sikidang Crater in Dieng geothermal field that producing chitin deacetylase enzyme, and to determine its activity characteristic of (optimum time production, optimum pH, and effect of 1 mM divalent metal ions) from the resulted chitin deacetylase enzyme. This research used completely randomized design. The data were analyzed using One Way ANOVA and Tukey HSD test. The results showed that KSR HA 24 isolates were able to produce chitin deasetylase with optimum enzyme activity of 0.668 U / ml at 18 hours production time. Optimum activity of chitin deacetylase occurred at pH 5 of 0.75 U / ml. Chitin deacetylase activity with 1 mM addition of divalent metal ions produce activator metal ions, including Mg2+, which increased the activity up to 154.43%, Fe2+ the activity up to 144.63%, and Cu2+ the activity up to 110.41%. Inhibitor metal ions, including Zn2+, which decreased the activity to 93.77%, and Mn2+ the activity to 86.46%.Keywords: Chitinolytic, Chitin Deacetylase, Enzyme Activity, pH, Divalent Metal Ions
AKTIVITAS INHIBITOR α-GLUKOSIDASE ISOLAT KAPANG ENDOFIT DUWET (Syzygium cumini (L.) Skeels) Roseliana Fitri; Agung Suprihadi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Java plum known to have the ability as a diabetes drug. The essence of diabetes treatment is to maintain blood sugar levels in normal state using α-glucosidase inhibitor. Such capabilities can be obtained from endophytic mould found in java plum plant. The aim of this study is to get the best isolates that have the ability of α-glucosidase inhibitors with different carbon sources. This study uses java plum endophytic mould isolates which tested the ability of α-glucosidase inhibitor by using spectrophotometric techniques with breaking substrates to produce colored products and a variety of carbon sources such as sucrose, lactose, maltose and starch. The results of α-glucosidase inhibitor activity indicate that crude extract of isolates JB 2.3 has the best ability to produce α-glucosidase inhibitor with a percentage of 13.76% is higher than the positive control acarbose 1%. Lactose be the best carbon source with a mean inhibition of (19.27%), sucrose (13.58%), starch (12.70%), Maltose (10.76%).Keywords: α-glucosidase inhibitor, endophytic mould, java plum, diabetes melitus.
MICROBIAL FUEL CELL (MFC) MENGGUNAKAN BAKTERI BACILLUS SUBTILIS DENGAN SUBSTRAT LIMBAH SEPTIC TANK SERTA PENGARUHNYA TERHADAP KUALITAS LIMBAH Fitra Adi Prayogo; Agung Suprihadi; Budi Raharjo
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The power plant was still dependent on non-renewable energy is one factor in Indonesia fixed with the problems of electric energy crisis. Several lecturer have shown that B. subtilis can produced electricity through Microbial Fuel Cell (MFC). The purpose of this study is to measured the values of voltage, electric current, and power density as well as changes in the quality of waste water septic tank. Waste in a vacuum toilet can be of service "Doremon Jaya", sterilized and counted the value of the quality of wastes. B. subtilis inoculum was then inoculated into a bioreactor containing sterile waste septic tank with 4 different concentrations of 0%, 1%, 2% and 3% (v/v). Inoculum was incubated for 12 hours prior to observing. The result showed that B. subtilis with inoculum concentration of 3% (v/v) produced the greatest voltage, which is 299.70 mV on 4-hour with strong current and power density 0.2997 mA 8.98 mW / cm2. COD can reduced by up to 31.18% at the inoculum concentrations of 3% (v/v), while the value of BOD reduced by up to 25.03% at the inoculum concentrations of 3% (v/v). DO value also experienced the percentage decreased to 34.25% or decreased to 0.96 at the inoculum concentrations of 2% (v/v). pH value in the treatment of inoculum concentrations of 0%, 1%, 2% and 3% (v / v) decreased after the fermentation was consecutively 5.4, 4.8, 4.9, and 4.2  Kata kunci : Bacillus subtilis, Microbial Fuel Cell, Septic  tank, Wastewater quality
Produksi Pigmen Kapang Monascus sp. dari Angkak pada Substrat Tongkol Jagung (Zea mays) dengan Variasi Ukuran Substrat dan Kadar Air Sesaria Esa Sekar Ardini; Agung Suprihadi; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Natural dyes in food industry is more demand so its pushes eforts for developing the production. Monascus is one of fungi that is potentially used for anka production that used in industrial products such as meat, fish, soy sauce, and liquor. The aim of this study was to produce pigment from Monascus using corncob with 35 mesh (<0,5 mm), 18 mesh (0,6-1 mm), 10 mesh (1,1-2 mm) particle size substrate and 20%, 40%, 60% moisture content. The production of Monascus sp. pigments made of solid-state fermentation and  incubated for  14 days. The parameters observed were pigment production and biomass. Analysis of pigment using spektrophotometer with wavelength (λ)  of 500 nm as well as biomass analysis by measuring N-acetyl glucosamin. The results of analysis showed that particle with size 0,6-1 mm from the substrates and with 20% and 40% moisture content was the best condition in process of pigment production using corncob substrate. The production of pigment from fungi with substrate with size 18 mesh ((0,6-1 mm) in 20% moisture content reached 1,1 CVU (Color Value Unit)/ml, and  in 40% moisture content reached 1,07 CVU/ml. The highest biomass was reached on 10 mesh (1,1-2 mm)  particle size at 20% moisture content reached 0,83 A (absorbance)/gds (gram dry substrates), 40%  reached 0,72 A/gds, and 60% reached 0,81 A/gds.Keywords: Solid-state fermentation, corncob, Monascus sp., particle size, moisture content
PRODUKSI INULINASE OLEH KHAMIR Pichia manshurica DUCC Y-015 PADA TEPUNG UMBI DAHLIA (Dahlia variabilis Willd.) DENGAN VARIASI KONSENTRASI MnSO4.H2O DAN WAKTU INKUBASI Berlian Abadianti; Agung Suprihadi; w wijanarka
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Inulinase (E.C. 3.2.1.7) is an enzyme which able to hydrolyze an inulin into fructose and fructooligosaccharides.  The main application of inulinase enzyme in the food industries is as the ingredient in producing High Fructose Syrup (HFS). Moreover, the other important applications of inulinase enzyme are to produce ethanol, inulooligosacarida (IOS), fruktooligosacarida (FOS), pullulan, sorbitol, etc.  Pichia manshurica DUCC Y-015 is kind of yeast that is capable in producing inulinase in medium containing inulin. Optimization of inulinase enzyme production needs to be done to increase inulinase production, the way that could be conducted is by the addition of metal ion and optimization of incubation time. The purpose of this research is to investigate the effect of adding mangan ion (MnSO4.­H2O) and incubation time. In conducting this study, the researcher applies experimentally research by using Randomized Complete Block Design (RCBD) factorial pattern. The first factor is concentration of MnSO4.H2O, with concentration level 0 mM (M0), 0,1 mM (M1), 0,5 mM (M2). The second factor is the variation of incubation time, i.e. 6 hours (I6), 12 hours (I12), and 18 hours (I18) with three times repetition. The collected data were analyzed using ANOVA 5% signification (α= 0,05) and completed by the Duncan test. The result of analysis shows that variation of MnSO4.H2O concentration and incubation time does not significantly influential on inulinase activity of Pichia manshurica DUCC Y-015.Keywords: Inulinase, Inulin, Pichia manshurica DUCC Y-015, MnSO4.H2O, Incubation time.
Fermentasi Kopi Robusta (Coffea canephora) Menggunakan Isolat Bakteri Asam Laktat Dari Feces Luwak Dengan Perlakuan Lama Waktu Inkubasi Doni Usman; Agung Suprihadi; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Coffee is one of Indonesian main corps commodity. Caffeine content of robusta coffee is 1.6%-2.4% higher than arabica coffee that was 0.9-1.2%. Coffee fermentation was one of method to decrease caffeine content. Luwak coffee is coffee that produces by luwak use their digest system in luwak body and defecates as faeces. Lactic acid bacteria (LAB) exploration from luwak faeces that fed by robusta coffee is one of method to get lactic acid bacteria. This research aimed was lactic acid bacteria isolation from luwak faeces and determine best incubation time on robusta coffee fermentation. Bacteria isolation was resulted 27 isolate. Biochemical test done by Gram Staining Test, Catalase Test, Motility Test, and Acid Producing Test and resulted 13 LAB isolate. Characterization test done to all of LAB isolate and resulted best 3 isolate. Isolate M6 was xylanolitic potency, M8 was Proteolytic potentcy and m16 was cellulolytic potency. Robusta coffee fermentation done used mixed of M8, M6 and M16 as inoculum. This research use Complete Random Design (RAL), the treatment was fermentation time 8 hour (D1), 16 hour (D2) and 24 hour (D3), each treatment replays 3 times. Kontrol of this fermentation is robusta coffee without fermentation treatment (control 1) and luwak robusta coffee  (control 2). Data analysed by Analysis of Variance (ANOVA) continuing by Duncan Test with significance rate was 95%. This research result shown that fermentation time duration was cause decrease of caffein content, coffee beans weight and fermentation fluid pH. Highest caffeine content on D1 was 0.95%, and D3 was 0.72 %.Keyword: Luwak faeces, lactic acid bacteria, gram staining, fermentation, exploration
ISOLASI DAN KARAKTERISASI KAPANG ENDOFIT DARI PEGAGAN (Centella asiatica (L.) URBAN) Nur Sabrina Hasyyati; Agung Suprihadi; Budi Raharjo; Kristiani Dwiatmi
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

        Endophytic mold live in colonies in healthy plant tissues without causing damage to the host plant. Various types of plants can be potentially as a source of endophytic mold isolates. Plants that are hosts for the mold endophyte one of them is gotu kola (Centella asiatica (L.) Urban). This study aimed to isolate and identify the molecular of endophytic molds from gotu kola. Isolation of endophytic mold through the stages namely stage surface sterilization and purification. Mold isolates obtained later in the identification of the molecular basis using ITS region. DNA amplification using the primers ITS ITS 4 and 5. Ribbon successfully amplified DNA with a size of 600 bp. The results of molecular identification and phylogenetic analysis based BLAST. The results of the analysis of BLAST showed similarities each - each isolates with species of mold on genebank known isolates BiogenCC M1 and BiogenCC M25 with Phoma sp., Isolate BiogenCC M6 with    Colletotrichum siamense, isolates BiogenCC M10 is Colletotrichum destructivum, isolates BiogenCC M15 and BiogenCC M28 with Mycoleptodiscus indiscus, and isolates BiogenCC BiogenCC M18 and M19 with Fusarium oxyporum. Keywords: Mold endophyte, Moleculer identification, ITS, Pegagan.
Ekstraksi Protein dari Escherichia coli BL21 Rekombinan Gen Mycobacterium tuberculosis dengan Variasi Waktu Inkubasi Induksi Isoprophyl-β-D-Thiogalactosidase (IPTG) dan Metode Lisis Sel Nabila Swarna Puspa Hermana; Endang Kusdiyantini; Agung Suprihadi; Neny Nuraini
Jurnal Akademika Biologi Vol. 4 No. 2 April 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bacillus Calmete-Guerin vaccine is no more effective in preventing tuberculosis. The vaccine developed now is vaccine recombinant using Mycobacterium tuberculosis gen cloned in Escherchia coli to produce protein which is used as antigen compound in vaccine. The protein produced an intercellular protein which is needed cell lysis process so that the protein could be extracted. The aim of this research was to investigate the incubation time, and the optimum cell lysis method in protein recombinant extraction. Recombinant E. coli BL21 was incubated for 4, 8, 12, and 16 hours, and followed by, freeze thaw, sonication, and beads vortex lysis method. SDS PAGE quantification showed that the sample with 12 hour incubation with freeze thawing lysis method had the highest protein recombinant concentration by 4.327.870,4 pixel. Keywords: E. coli BL21 recombinant, protein extraction, enzymatic, freeze thaw, sonication, beads vortex
AKTIVITAS INHIBITOR Α-AMILASE ISOLAT KHAMIR ENDOFIT DARI TUMBUHAN BROTOWALI (Tinospora crispa L.) Pramita Dian Pramitasari; Sri Pujiyanto; Agung Suprihadi
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Diabetes mellitus is a carbohydrate, fat, and protein metabolism disorder characterized by high blood sugar level. The α-amylase inhibitor may inhibit the carbohydrates digestion and cause a rate decrease of glucose absorption. The α-amylase inhibitors are found in a plant that potentially containing antidiabetic compounds, such as Brotowali (Tinospora crispa L.). The exploration of endophytic microbial is expected can produce secondary metabolites with similar properties to those produced by host plant metabolites. The purpose of this research were to obtain endophytic yeast isolates which can produce the best α-amylase inhibitors and to determine the ability of selected isolates to produce α-amylase inhibitors on varied carbon source and pH production media. This research was conducted by isolating endophytic yeast from roots, stems, and leaves of brotowali. The best isolate was selected by considering its inhibitory activity. The α-amylase inhibitor test was determined by measuring the resulting reduction sugar. This research used Completely Randomised Design. First treatment was carbon source media, such as starch, sucrose, maltose and lactose. The best carbon source then was used for further tests. Second treatment was pH media including pH of 5, 6, 7 and 8. The data obtained later were analyzed using One Way ANOVA. The selection results showed that  DG26 isolate had the best α-amylase inhibition value of 68.27% with 72 hours optimal time production. The statistical result test on the varied carbon source treatment showed insignificant effect (P> 0,05) with the best α-amylase inhibition value of 19,98% at starch treatment. The statistical result test on the varied pH treatment showed a significant different effect (P <0.05) with the best α-amylase inhibition value of 44.91% at pH 7.Keywords: α-amylase inhibitor, endophytic yeast, Tinospora crispa, carbon source, pH
Produksi Pigmen Merah Monascus sp. pada Substrat Tepung Biji Durian (Durio zibethinus Murr.) dan Biji Nangka (Artocarpus heterophyllus Lam.) dengan Variasi Penambahan Sumber Nitrogen Tsania Dyna Falasifa; Agung Suprihadi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Public awareness about the danger of using synthetic dyes impact on health for a long term, pushes the demand of natural dye that is healthier. One of the natural dye is Monascus sp. red pigment which traditionally uses the rice substrate. Durian seed and jackfruit seed are residue from agroindustry which still have high nutritional value thus potentially become the alternative substrate. The aim of this study was to measured Monascus sp. red pigment production in the flour substrate of durian seed and jackfruit seed with variant nitrogen source added. This research used completely randomized design (CRD) factorial with 2 factors. The first factor was a kind of substrate fermentation (N): jackfruit seed flour and (D): durian seed flour. The second factor was concentration peptone added (P0): 0%, (P1): 1%, (P2): 2%, and (P3): 3%. Research consist of 8 treatments with 3 replications. Research parameters were red pigment concentration and biomass estimation. Analysis of the data used Analysis of Variance (ANOVA) followed by Duncan’s Multiple Range Test (DMRT) at the 95% significance level and Pearson correlation test. The result showed that Monascus sp. can  grew and producted red pigment on both of substrate with the addition of a nitrogen source variation. Fermentation on the jackfruit seed flour substrate with 2% peptone added was the most effective in red pigment production  with 5,55 CVU/gds. The addition of 2% peptone on both types of substrate yielded highest biomass with 1.53 mg/gds, and there was a positive correlation between the biomass and the red pigment production. Keywords: Pigment, Monascus sp., Durio zibethinus, Artocarpus heterophyllus, nitrogen
Co-Authors AB SUSANTO Anindita Ayu Pratiwi Aniza Rachmawati Anto Budiharjo Arina Aisyah Arina Tri Lunggani Baedah Rupaedah Barri Pratama Berlian Abadianti Budi Raharjo Debby Widiyanti Doni Usman Endang Kusdiyantini Genoveva Preta Angelika Ghaida Afra Akhsani Griffin Natassya Kristiani Dwiatmi Laksono Trisnantoro Linda Ayu Utami Linda Safitri M Eka Prastya Mamik Setyowati Maya Fitriana Ilul Fahmi MG Isworo Rukmi Nabila Swarna Puspa Hermana Neny Nuraini Nur Sabrina Hasyyati Pramita Dian Pramitasari Prayogo, Fitra Adi PRIYO WAHYUDI Reza Auliarahman Bhaktinagara Roseliana Fitri Rutty Wulandari Sesaria Esa Sekar Ardini Sih Parmiyatmi Siti Nur Jannah Sona Fatimah Sri Pujiyanto Thu&#039;ti Alawiyyah Tri Wijiastuti Tsania Dyna Falasifa w wijanarka Yeti Darmayati