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Mamik Setyowati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Environmental Science Industrial & Manufacturing Engineering Mechanical Engineering

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PENAPISAN DAN PEMANFAATAN RHIZOBAKTERI TANAMAN SORGUM (Sorghum bicolor L. Moench) SEBAGAI INOKULAN PEMACU TUMBUH TANAMAN Annisaa Widyasari; W Wijanarka; Budi Raharjo; Mamik Setyowati
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.196 KB)

Abstract

Sorghum was a cereal crop that has many benefit such as food, feed, industrial, and bioenergy. Sorghum had a potency to be cultivated, but productivity of sorghum was still low both in quatity and quality. One way to increase production of sorghum  is using rhizobacteria as biofertilizer. The aim of this study is to get rhizobacteria that has the ability to produce IAA, solubility of phospat (P), Nitrogen (N) fixing, and analyze the effect of rhizobacteria inoculants for enhance sorgum plant growth. Isolation of rhizobacteria was done by diluting  rhizobacteria sorghum suspension from 10-1 to 10-5 and it were be platted on SEA medium. Isolates were screened by ability to produce IAA, solubility of P, and N fixing. Producing of IAA test was done by adding Salkowsky reagent on bacterial supernatant and measured absorbance at 530 nm wavelength. Solubility of P test was done by inoculating isolates in Pikovskaya media, while N fixing test was done on N fixing media (NFB). Isolates of rhizobacteria which had a potency to increase growth of plants were made inoculants to be applied in sorghum plants. The result of this study obtain 3 isolates i.e Sr 194.3; Sr 172.1; and Sr 209.1 which were considered effective for increase growth of sorghum. The conclusion  of this study isolates which showed the highest average plant height, root length, and dry weight Sr 194.3 isolate. The statistical analysis among the treatments showed that did not any significant differences on plant height, root length, and dry weight of sorghum age 28 days after farming. Keyword : Increase growth plants, Screening, Shorgum, Rhizophere.
PENAPISAN DAN PEMANFAATAN RHIZOBAKTERI TANAMAN JAGUNG (Zea mays) SEBAGAI INOKULAN PEMACU TUMBUH TANAMAN Debby Widiyanti; Sri Pujiyanto; Agung Suprihadi; Mamik Setyowati
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Maize (Zea mays) is the second most important food commodity after rice plants. The role of the corn crop in agricultural commodities encourage the use of biological fertilizers to increase crop productivity of maize. The use of biological fertilizers open opportunities using rhizosphere bacteria as inoculants boosters grow plants.This study aims to obtain bacterial isolates from the rhizosphere of maize (Zea mays), characterize the morphology and biochemical properties of the ability to produce IAA, provide nutrients such as N and P and to get a bacterial inoculant boosters grow corn. Rhizosphere bacteria were isolated using SEA media (soil extract agar). Isolates that have the ability to produce IAA, tether N and high P solvents tested against corn seed germination and applied in a phase of plant growth. The results showed there were 24 isolates corn crop rhizosphere dominated by gram-positive bacteria and shaped basil capable of producing IAA grading 4,83 to 125,84 ppm. 16 rhizosphere bacterial isolates capable of dissolving phosphate with phosphate solubility index ranged from 2,1 to 4,6. 18 rhizosphere bacterial isolates capable of tether N with the highest N tether capacity of 2,8 x 10 3 CFU/ mL. Application 3 selected bacteria such as J11, J16 and J19 as inoculants are not able to increase the growth of corn plants compared to control water. Keyword :Maize, Rhizosphere, The growth of corn plants.
Analysis of Tea Rhizosphere Bacterial Community at the Seedling Stage Using Terminal Restriction Fragment Length Polymorphism (TRFLP) Techniques Dwi Ningsih Susilowati; Fani Fauziah; Eko Pranoto; Ernin Hidayat; Mamik Setyowati; Yati Rachmiati
Jurnal Penelitian Teh dan Kina Vol 19 No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v19i2.114

Abstract

Bio-imunizer contains an active compound of  Chryseobacterium sp. and Bacillus sp. has been developed by PPTK Gambung. This formula has positive effect on the growth of tea plants also potentially increasing resistance of the plant. The purpose of this study was to determine the effects of bacteria in Bio-imunizer to the rhizosphere bacterial communities as well as the consistency of its existence after application on tea plants at the nursery stage. The technique used in this research is Terminal Restriction Fragment Length Polymorphism based on metagenomic and culture dependent approaches. The value of relative abundance, Shannon diversity index, Pielou's evenness index, and Simpson dominance index were calculated. Based on the T-RF profiles of rhizosphere bacterial communities show that Chryseobacterium sp. and Bacillus sp. which is the active compound of  Bio-imunizer consistently found in the tea plant rhizosphere. Application of Bio-imunizer can increase the diversity of rhizosphere bacterial community without affecting the communities that already exist.
Co-Authors Agung Suprihadi Annisaa Widyasari Budi Raharjo Debby Widiyanti Dwi Ningsih Susilowati Eko Pranoto Ernin Hidayat Fani Fauziah Sri Pujiyanto w wijanarka Yati Rachmiati