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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia Berkala Perikanan Terubuk Jurnal Ilmu Ternak dan Veteriner Animal Production : Indonesian Journal of Animal Production
W Manalu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Heat stress effect and given of hexane extract jaloh bark (Salix tetrasperma Roxb) to cortisol level, triiodothyronine and hematology profile of broiler chickens ., Sugito; Manalu, W; Astuti, D.A; Handharyani, E; ., Chairul
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 3 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.3 KB) | DOI: 10.14334/jitv.v12i3.482

Abstract

Plasma concentration of cortisol and hematological profile on broiler chickens can be used as heat stress indicators. This study was conducted to investigate the effect of administration of n-hexane extract of jaloh bark (EHJ) on heat-stressed broiler chicken. Thirty broilers, aged 20 days (strain Cobb), were randomly divided into 5 groups. The first group was external control (tCP) i.e. chickens without heat stress and without EHJ administration. The second group was internal control (CP) representing chickens given heat stress without EHJ administration. The third, fourth, and  fifth groups were chickens given heat stress and EHJ administration at doses 5, 10, and 20 mg/kg BW, respectively symbolized as CP+EHJ5, CP+EHJ10, and CP+EHJ20. The experimental broilers were exposed to heat stress in constant temperature of 33 ± 1oC for 4 hours daily for 5 and 10 days. The EHJ was given 1 hour before temperature in the cage was raised and was given in daily basis.  The level of cortisol in feces were measured from feces collected 1-2 hours before  treatment, 3-4 hours after heat stress exposure, and 2-3 hours after heat stress termination. This research indicated that heat stress at temperature 33 ± 1oC during 2 and 4 hours increased cortisol  excretion in feces (P<0.05) and increased ratio H : L (P<0.10). The EHJ at dose of 10 mg/kg BW relatively gave more protection from heat stress impact on broilers. Key Words: Cortisol, Triiodothyronine, Heat Stress, Salix, Hematology
Histopathology of liver and kidney on broiler chicken exposed to heat stress and fed extract of Jaloh (Salix tetrasperma Roxb) ., Sugito; Manalu, W; Handharyani, E; ., Chairul
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.588 KB) | DOI: 10.14334/jitv.v12i1.566

Abstract

This research was conducted to study the effects of jaloh extract (Salix tetrasperma Roxb) on broiler chicken exposed to heat stress in constant temperature of 33 ± 1oC for 4 hours per day for 10 days. Fifteen chickens aged 20 days old (strain Cobb) were divided randomly into 5 groups. The first group was external control (tCP) of the chickens received neither heat stress nor jaloh extract. The second group was internal control (CP) representing chickens given heat stress without jaloh extract. The third, fourth, and fifth groups consisted of chickens given heat stress and 10 mg/kg BB of hexane (CP+Hek), ethyl acetate (CP+EtOAc), and ethanol (CP+EtOH) fractions, respectively. Heat stress and jaloh extract were given every day. Extract of jaloh were given 1 hour before temperature in the cage was raised. On 10th day of the experiment, 3 chickens were killed and necropted. Liver and kidney tissues from each treatment were analyzed for histological condition. The results indicated that heat stress can induce injury degeneration, necrosis, and infiltration of inflammation cells of liver and kidney. It is concluded that the hexane fraction of jaloh extract could protect the detrimental effects on heat stress. Key Words: Heat Stress, Extract Salix, Liver, Broiler Kidney
The effect of superovulation prior to mating on fetal growth in Iambs from Javanese thin-tail ewes Manalu, W
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 4 (1999)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.867 KB) | DOI: 10.14334/jitv.v4i4.166

Abstract

Twenty-nine fetuses (11 fetuses from 9 non-superovulated ewes and 18 fetuses from 8 superovulated ewes) were used to study the effect of superovulation of ewes prior to mating on fetal weight, fetal length, the length of the body and limbs, chest circumference, weights of the body, head, neck, limb, and viscera. Superovulated ewes, though with a higher litter size, had a greater fetal growth as was indicated by the greater fetal weight and length, the length and weight of the body and limb on day 49 of pregnancy. On day 105 of pregnancy, superovulated ewes with multiple fetuses (≥3) had similar fetal growth than nonsuperovulated ewes with single and twin fetuses. However, superovulated ewes with a single fetus had greater fetal growth as was shown by the greater fetal weight and length, the length of the body and limbs, chest circumference, and weight of the body, limb, and viscera when compared to those non-superovulated ewes with a single or twin fetuses. The results of the experiment suggested that superovulation of ewes prior to mating could be used to improve fetal prenatal growth during pregnancy   Key words: Superovulation, fetal growth, pregnancy, sheep
The effect of Tea Misletoe (Scurrula oortiana) Stem Extract as Immuno-Modulator on Oncogenic Marek’s Disease Virus Infection Samsi, Mulyoto; Malole, MBM; Manalu, W; Handharjani, E
ANIMAL PRODUCTION Vol 9, No 3 (2007): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Marek’s disease virus (MDV) is one of oncogenic herpesvirus. It causes immunosupresion and cancer in chicken. Several plants produce bioactive compounds which are very useful for treatment of many disease, especially hiperproliveration and virus infection. This study was aimed to find out mechanism of immuno-modulatory capacity in layer commercial chicken administered orally with extract of tea parasite (Scurrula oortiana) in dose of 10 mg/kg BW through drinking water, then the chicken were infected by intraperitoneal oncogenic MDV in dose of 1,0 x103 TCID50. The study used 60 layer commercial day old chicks (DOC) divided into four group treatments. The treatments were group A (administered S. oortiana extract and without MDV infection), B (neither S. oortiana nor MDV infection), C (administered S. oortiana extract and with MDV infection), and D (without administered S. oortiana extract, but with MDV infection). Results showed that MDV oncogenic caused immunosupresion at a day post infection (p.i) and recovery to be normal based on relative weight of bursa Fabricius and thymus at 40 days p.i. The extract of S. oortiana had a capability as an immunomodulator indicated by the increase of relative weight of bursa Fabricius and thymus at day 20 days p.i. (Animal Production 9(2): 172-177 (2007) Key Words: Marek’s disease virus (MDV), Scurrula oortiana, immuno-modulator
Improvement of Lamb Preweaning Performance by Combination of Superovulation of Ewes Prior to Mating and Temulawak Extract Plus Administration During Pregnancy Andriyanto, Andriyanto; Darulfallah, MD; Arif, R; Nugraha, GM; Winarto, A; Manalu, W
ANIMAL PRODUCTION Vol 14, No 3 (2012): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Abstract. Superovulation is one of a reproductive technology to improve livestock productivity. The research was conducted to optimize the superovulation technology by combining it with administration of temulawak extract plus during pregnancy. Sixteen ewes were injected prostaglandin hormone at a dose of 10 mg/kg body weight intramuscularly twice, with eleven days interval, to synchronize estrous cycle. On the eleventh day, superovulation was  induced by injection of pregnant mare serum gonadotrophin  hormone at a dose of 200 IU/ewe intramuscularly. The ewes showing the estrous signs were mated naturally. Temulawak extract plus was administered weekly during pregnancy with a dose of 1 mg/kg body weight. Parameters measured in this study were lambs birth weight and preweaning growth. Superovulation increased average litter size. The result showed that, regardless of litter size, superovulation increased lambs birth weight by 15% as compared to controls and temulawak extract plus groups. Superovulation before mating and temulawak extract plus administration during pregnancy improved lambs growth in the first month and the third month postpartum. Superovulation prior to mating increased lambs birth weight and improved lambs growth performance before weaning. Keywords: superovulation, temulawak extract plus, lambs growth performance, ewes Abstrak.  Superovulasi merupakan salah satu teknologi reproduksi untuk meningkatkan produktivitas ternak. Penelitian ini dilakukan untuk mengoptimalkan teknologi superovulasi dengan menggabungkan pemberian ekstrak temulawak plus selama kebuntingan. Enam belas domba disuntik hormon prostaglandin dengan dosis 10 mg/kg bobot badan secara intramuskular sebanyak dua kali, dengan selang waktu sebelas hari, untuk menyesuaikan siklus estrus. Pada hari ke sebelas, superovulasi diinduksi dengan injeksi pregnant mare serum gonadotrophin hormon dengan dosis 200 IU/domba secara intramuskuler. Domba yang menunjukkan tanda-tanda estrus kemudian dikawinkan secara alami. Ekstrak temulawak plus diberikan setiap minggu selama kebuntingan dengan dosis 1 mg/kg bobot badan. Parameter yang diukur dalam penelitian ini adalah bobot lahir anak domba dan pertumbuhan prasapih. Superovulasi meningkatkan rata-rata litter size. Hasil penelitian menunjukkan bahwa, terlepas dari litter size, superovulasi meningkatkan bobot lahir anak domba sebesar 15% dibandingkan dengan kelompok ekstrak temulawak plus. Superovulasi sebelum kawin dan pemberian ekstrak temulawak plus selama kebuntingan  meningkatkan pertumbuhan anak domba di bulan pertama dan bulan ketiga paska melahirkan. Superovulasi sebelum perkawinan meningkatan berat lahir anak domba dan meningkatan performa pertumbuhan anak domba sebelum penyapihan. Kata kunci: superovulasi, temulawak ekstrak plus, kinerja pertumbuhan anak domba, domba Andriyanto et al/Animal Production 14(3):167-172, September 2012
Physical and Chemical Characteristics of Maleo Egg in Bogani Nani Wartabone Park Saerang, JLP; Manalu, W; Soesanto, IRH; Mardiastuti, A
ANIMAL PRODUCTION Vol 12, No 1 (2010): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The objective of this study was to examine the size (weight, length, and width) of Maleo egg in Bogani Nani Wartabone National Park.Physical characteristics that measure are : egg weight, egg shell, egg index , yolk, and albumen. Physical characteristics measured were: egg weight, egg shell thickness, egg index, yolk, and albumen. Two fresh eggs were weighed to the electric ohause balance with 400 g the scale 0.01. Two fresh egg were weighed with an electric balance. Length and width off egg were measured by used vernier caliper, egg shell thickness by  tripod micrometer, yolk color by yolk color fan. Length and width of egg used were measured by Vernier caliper, and egg shell thickness by a tripod micrometer, yolk color by yolk color fan. Physical characteristic have been identified eggs weight, egg width, egg length, egg index, albumen width, albumen length, albumen index, yolk width, yolk length, yolk index, weight albumen, weight yolk, egg shell thickness, and shell weight increased by 223.70 g, 104.00 mm, 56.68 mm, 56.41, 113.8 mm, 86.72 mm, 6.07%,  85.30 mm, 69.90 mm, 31.54%, 47.51 g, 110.80 g, 0.02 inch, 19.55 g, respectively. The result of analysis indicated that maleo eggs have completed amino acids and fatty acids, that is essensial amino acids, non essensial amino acids, saturated fatty acids and unsaturated fatty acids. Physical characteristic egg that comprised of egg weight, egg width, egg length, egg index, albumen width, albumen length, albumen index, yolk width, yolk length, yolk index, albumen weight, yolk weight, egg shell thickness, and shell weight were 223.70 g, 104.00 mm, 56.68 mm, 56.41, 113.8 mm, 86.72 mm, 6,07%, 85.30 mm, 69.90 mm, 31.54%, 47.51 g, 110.80 g, 0:02-inch, 19:55 g, respectively. Maleo egg contained complete amino acids and fatty acids, including essential amino acids, non essential amino acids, saturated fatty acids and unsaturated fatty acids. (Animal Production 12(1): 34-38 (2010)Key Words: Maleo bird, maleo egg, physical characteristics, chemical characteristics
The Effects of Superovulation Prior to Mating and Zinc Supplementation on Milk Yields in Etawah-Grade Does Adriani, Adriani; Sutama, IK; Sudono, A; Sutardi, T; Manalu, W
ANIMAL PRODUCTION Vol 6, No 2 (2004): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Thirty six Etawah-grade does (BW ranged from 20.4 to 44.2 kg and age ranged from 2.5 to 7 years) were used to study the effects of superovulation prior to mating and zinc supplementation on milk yields. The experimental does were assigned into a randomized block design with a 2 x 3 factorial arrangement. The first factor was PMSG (pregnant mare serum gonadotrophin) injection with 2 levels (0 IU/kg BW [nonsuperovulation–NSO] and 15 IU/kg BW [Superovulation–SO]). The second factor was zinc concentration in the diet with 3 levels (40 mg/kg DM [Z-40], 60 mg/kg DM [Z-60], and 80 mg/kg DM [Z-80]). Intravaginal sponge (60 mg medroxyprogesterone acetate) was applied for 14 days to synchronize estrus cycle. Twenty four  hours prior to sponge removal, PMSG was injected  to  stimulate superovulation. After  sponge removal,  5 experimental does  were mixed with 1 buck for natural mating. During  pregnancy  the experimental does were fed and  raised in   individual stables. Udder volume  was measured every other week from weeks 12 to 21 of  pregnancy.  During lactation  (one week to  5 months post partum) kids were separated from the does, and   milk was harvested by hand milking. Milk samples were taken every other week for determination of milk quality. The results  indicated that  superovulation increased udder differential growth  by 80% (822.85 vs 1481.25 cm3/head), milk yield by  32% (567.14 vs 746.52 g/head/day).  Zinc concentration  increased udder differential growth and milk yield. Milk productions for does receiving 40, 60 and 80 mg/kg DM were 565.68, 737.88 and 666.92 g/day, respectively.It was concluded that  superovulation and zinc supplementation  increased milk  yield in Etawah Grade does. (Animal Production 6(2): 86-94 (2004) Key Words:  Superovulation, Zinc, Milk  Yield, Etawah-Grade
Increasing Goat Productivity Through the Improvement of Endogenous Secretion of Pregnant Hormones Using Follicle Stimulating Hormone Andriyanto, Andriyanto; Manalu, W
ANIMAL PRODUCTION Vol 13, No 2 (2011): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Abstract. Previous studies reported that the improvement of endogenous estrogen and progesterone secretions during gestation improved fetal prenatal growth, birth weight, mammary gland growth and development, milk production, litter size, pre- and post-weaning growths. An experiment was conducted to apply the improvement of endogenous secretion of pregnant hormones during pregnancy to increase goat productivity. Thirty-six female ettawah-cross does were divided into 2 groups. Group 1 (control: 18 does) included does without improvement of endogenous secretion of pregnant hormones and Group 2 (treatment: 18 does) included does with improvement of endogenous secretion of pregnant hormones using follicle stimulating hormones to stimulate super ovulation. The application of this technology increased total offspring born (control: 25 offspring; treatment: 42 offspring), average litter size (control: 1.88; treatment: 2.33), offspring birth weight (control: 2.85±0.50 kg; treatment: 3.82±0.40 kg), and does milk production (control: 1.36±0.34 L/does/day; treatment: 2.10±0.21 L/does/day). Offspring born to does with improved endogenous secretion of pregnant hormones had better weaning weight (control: 11.17±1.99 kg/offspring; treatment: 14.5±1.11 kg/offspring). At weaning period, does with improved endogenous secretion of pregnant hormones produced offspring with total weaning weight twice as heavy as control does (control: 189.9 kg; treatment: 403.6 kg). By a simple calculation of economic analysis, this technology application could increase gross revenue per does until weaning by Rp. 432.888,89. It was concluded that this technology is economically feasible to be applied in small-scale farm.Key Words: follicle stimulating hormone, pregnant hormones, endogenous secretion, super ovulation, ettawah-cross does
Effect of Specific Lactate Dehydrogenase Antigen from Sheep with Different Prolific Type on Hematology and Antibody Responses of Chicken Sumaryadi, MY; Prayitno, Prayitno; Manalu, W
ANIMAL PRODUCTION Vol 9, No 1 (2007): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Maternal serum of thirty nine ewes were used to study hematological and antibody responses of chicken injected with antigen protein of specific LDH obtained from sheep with different littersizes (prolific type). The prolific type of ewes was determined base on littersize (LS), which were low ( 1 ≤  LS ≤ 1.5), medium ( 1.5 < LS ≤ 2.0), and high (LS > 2.0). There were ten ewes carrying specific LDH protein. The number of animals of low, medium, and high prolific-type ewes was 3, 5, and 2 heads, respectively. The serum was used to produce antigen and injected to chicken to promote antibody production of anti LDH. The results of research showed that different prolific-type has no significant (P>0.05) effects on hematological and antibody titer of chicken.  However, hematological status of chicken injected with specific LDH antigen was significantly (P<0.05) higher than control. It can be concluded that production of specific protein anti LDH can be done in layer by injection of antigen from ewes. Product of specific protein anti LDH of prolific ewes still needs field test for its accuracy for selection of ewes with different prolific type. (Animal Production 9(1): 40-44 (2007) Key Words: hematology, antibody, LDH, ewes 
Effect of Curcumma, Zn-Proteinate, and Cu-Proteinate Supplements on Milk Production of Subclinical Mastitis Fries Holland Cows Taspirin, DS; Makin, M; Manalu, W; Tanuwiria, UH
ANIMAL PRODUCTION Vol 12, No 1 (2010): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The objective of the research was to find out the effect of Curcumma, Zn-proteinate, and Cu-proteinate supplementation on subclinical mastitis status in term of 4% FCM milk production. The research was conducted using 24 heads of lactating dairy cows in Randomized Block Design with six treatments, and four groups of milk production as replication i.e. I=  >14 kg/day ; II= 12-13.99 kg/day ; III= 10-11.99 kg/day;  IV= <10 kg/day.  Treatments were:  R1 (Control); R2 (R1 + 2% Zn proteinate); R3(R1 + 2% Cu-proteinate); R4(R1 + 2% Curcumma); R5(R1 + 2% Zn-proteinate + 2% Cu-proteinate); R6 (R1 + 2% Zn-proteinate + 2% Cu-proteinate + 2% Curcumma). Parameters observed were 4%FCM milk production and subclinis status.  The results showed that ration supplemented with Curcumma, Zn-proteinate, and Cu-proteinate decreased mastitis subclinic status and 4% FCM milk production increased significantly.  Supplementation of Curcumma, Zn-proteinate, and Cu-proteinate resulted is the best for  decrease in subclinical mastitis indicator and increase 4% FCM milk production. (Animal Production 12(1): 16-20 (2010)Key Words :   dairy cows, subclinic mastitis, curcumma, Zn-proteinate, and Cu-proteinate
Co-Authors . Adawiah . Chairul A Anang A Mardiastuti, A A Sudono, A A Sutisna A Winarto A.A. Ketut Agung Cahyawan W ADRIANI ADRIANI Andriyanto &#039; Andriyanto Andriyanto Chairul . Chairul . D A Astuti D.A Astuti D.T H Sihombing DS Taspirin, DS E Handharjani, E E Handharyani GM Nugraha, GM H Setiyatwan I Hernaman I Mokoginta IK Sutama, IK IRH Soesanto, IRH JLP Saerang, JLP K Suanda L Andini M Makin, M M. Y. Sumaryadi MA Suprayudi MBM Malole, MBM MD Darulfallah, MD Mia Setiawati MT Suhartono, MT Mulyoto Samsi, Mulyoto N Kusumorini, N N Ramli Nahrowi Nahrowi P I Pudjiono Prayitno Prayitno R Arif, R R Hidayat R Wiradimadja, R Revolson Mege S Valinata SH Nasution, SH Sugito Sugito T Sutardi T Sutardi T Toharmat TI Winarno U H Tanuwiria UH Tanuwiria, UH W G Piliang WG Piliang, WG