SYAFRIDA MANUWOTO
Institut Pertanian Bogor Jl. Dramaga, Bogor

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AKTIVITAS INSEKTISIDA BAGIAN TUMBUHAN CALOPHYLLUM SOULTTRI BURM.F. (CLU IACEAE) TERHADAP LARVA LEPIDOPTERA Syahputra, Edy; Manuwoto, Syafrida; Darusman, Latifah K; ., Dadang; Prijono, Djoko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 4, No 1 (2004): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1953.917 KB) | DOI: 10.23960/j.hptt.1423-31

Abstract

 The objective of this test was evaluate the insecticidal activity of ectract of some parts of Calophyllum soulattri (Clusiaceae) against larvae of three species of Lepidoptera, i. e Crocidolomia pavonana, Plutella xylostela, and Pieris sp.. Extraction of plant materials was done by infusion method using ethanol. The bioassays were conducted by leaf-feeding method. Second-instar larvae were fed extract-treated broccoli leaves of 48 hours, then they were presented with untreated leaves until the surviving larvae larvae reached the fourth-instar stage. The number of dead larvae was recorded daily an larval mortality date were analyzed by probit method. The result showed the gummy bark exudates and bark extract of old and young C. soulattri plants were highly active against C. pavonana. The abrk extact of old C. soulattri plant was also effective against P. xilostella and Pieris sp. The gummy exudates possessed strong insecticidal activity against C. pavonana larvae with LC50 of 0.04% and prolonged the developmental time from second to fourth instar of C. soulattri 2.03-7.25 days compared with control. The bark excudate gave positive respon to alkaloid flavonoid, and tannin test. Futher studies are needed to identify insecticidal compound in those active extracts.
TEST OF BLOOD DISEASE BACTERIUM (BDB) TRANSMISSION BY POTENTIAL INSECT VECTORS Sahetapy, Betty; Maryana, Nina; Manuwoto, Syafrida; H. Mutaqin, Kikin; Latumahina, Fransina
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 20 No. 1 (2020): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.12071-77

Abstract

Blood disease bacterium (BDB) is one of the important diseases in banana and a major obstacle in developing and increasing banana production in Indonesia. The purpose of this study was to prove the ability of the Drosophilidae insect as a vector in transmitting BDB. The research was conducted at the Insect Biosystematics Laboratory and Plant Bacteriology Laboratory, Department of Plant Protection, Faculty of Agriculture, IPB University. Drosophilidae insects were taken from the field and then reared in laboratory by being fed with ripe bananas to obtain offspring that are free from diseases or pathogens. Imago of the Drosophilidae from rearing was fed by inoculum sources which was infected banana, then inoculated into healthy plants. The plants used were healthy and flowering, heliconia. The results showed that the Drosophilidae insects were able to transmit BDB to heliconia plants that showed symptoms, brownish flower colors and falling flower crowns. Detection of BDB isolated from flower parts and the inside parts of the insects used in transmission test using the PCR method showed positive results.