Siti Muflichatun Mardiati
Jurusan Biologi, Fakultas Sains Dan Matematika, Universitas Diponegoro Jl. Prof. Soedarto, SH, Tembalang, Semarang

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Application of Snakehead Fish to Increase Liver Function of Rats after Experiencing Physiological Stress Sunarno, Sunarno; Mardiati, Siti Muflichatun; Rahadian, Rully
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13957

Abstract

Physiological stress due to nutritional deficiency accompanied by excessive activity can cause problems of energy deficiency required by the body. Energy shortage can affect the body weight, as well as the weight and histology of the liver. This study was conducted to examine and analyze the effect of snakehead fish meat supplementation in feed toward body weight, weight and hepatic histology in animals experiencing physiological stress. This study used 20 male Wistar rats consisting of 5 treatments with 4 replications. The treatment in this study consisted of control (P0) and treatment (P1: 5%, P2: 10%, P3: 15%, P4: 20%) groups. This research used Completely Randomized Design (RAL). The variables measured in this study were body weight, hepatic weight, central venous diameter and hepatocytes in the test animals. The data obtained were analyzed using Analysis of Variance (ANOVA) at 5% significance level and continued with Duncan test with 5% significance. The result of data analysis showed that fish snakehead fish supplementation in feed gave a significant effect to body weight, weight and histology of animal liver. Concentrations of snakehead fish supplements can increase the body weight, hepatic weight, and hepatic histology which was indicated by higher central venous and hepatocyte diameter values compared to other controls and treatments. The conclusion of this research is the provision of snakehead fish supplement (Channia striata) can increase the body weight, hepatic weight, central venous and hepatocytes diameter of Wistar rats with the most influential concentration of 20%. This evidence is useful in studying the mechanism of liver repair due to stress
Bobot, Indeks Kuning Telur (IKT), Dan Haugh Unit (Hu) Telur Ayam Ras Setelah Perlakuan Dengan Pembungkusan Pasta Rimpang Temulawak (Curcuma xanthorrhiza Roxb). Putri, Dyah Ayu Maharani; Djaelani, Muhammad Anwar; Mardiati, Siti Muflichatun
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (62.2 KB) | DOI: 10.14710/bioma.18.2.7-13

Abstract

Eggs are farm products that provide the greatest contribution to the achievement of community nutritional adequacy. The aims of the research was to an analyzed the quality of eggs in treatment without and with a temulawak rhizome pasta stored at 6,12, and 24 days. This research used  35 eggs consists of 7 treatment and 5 replication, namely PtT0 : as control, a group of eggs without packing temulawak rhizome pasta and without storage, PtT6 : a group of eggs without packing temulawak rhizome pasta after 6 days storage, PdT6 : a group of eggs with packing temulawak rhizome pasta after 6 days storage, PtT12 : a group of eggs without packing temulawak rhizome pasta after 12 days storage, PdT12 : a group of eggs with packing temulawak rhizome pasta after 12 days storage, PtT24 : a group of eggs without packing temulawak rhizome pasta after 24 days storage, and PdT24 : a group of eggs with packing temulawak rhizome pasta after 24 days storage. This research was analyzed used a completely randomized design (CRD). Variables measured were egg weight, yolk indeks, and Haugh Unit (HU). Data obtained from this study were analyzed using analysis of variance (ANOVA) if there is a real difference then continued further test using Duncan test at the level 95 %. The result  egg weight reducted and yolk index shows was significantly different in PtT6, PtT12, PtT24, and PdT24 and Haugh Unit (HU) shows was significantly different in PtT6, PtT12, PtT24. Conclusion of this research is gived temulawak rhizome pasta are able to maintain the quality of eggs were observed on days 6, 12, and 24. Key Word : Chicken egg,  Temulawak rhizome, Egg quality
Pertumbuhan Kelinci (Lepus spp.) Setelah Pemasangan Implan Stainless Steel AISI 316L Wicaksono, Wisnu Adi; Tana, Silvana; Mardiati, Siti Muflichatun
Bioma : Berkala Ilmiah Biologi Vol. 18, No.2, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.147 KB) | DOI: 10.14710/bioma.18.2.137-143

Abstract

A lot of accidents can cause fractures/cracks, especially in the thigh bone. Bone fractures/cracks can be overcome through implants installation (implantation) particularly on the part of the femur. A test take the implant integration process about 4-6 week. Research on animals is performed the rabbit (Lepus spp.) implantation of stainless steel AISI 316L. The implantationof stainless steel AISI 316L implant was on the right femur between thigh bone and sceletal muscle, that was observed for 1,5 months. Parameters observed in this research were body weight gain, feed consumption, conversion of feed, and drinking consumption. Test used is t two test samples independent two sides with take samples 5 control and 5 treatment stainless steel AISI 316L. The results show the stainless steel AISI 316L implantsignificant differences body weight gain, feed consumption, and drinking consumption,but no significant different feed conversion. The conclusion is an implant stainless steel AISI 316L influence body weight gain rabbits after implantation for 1.5 months. Keywords: Rabbit, stainless steel AISI 316L, growth, body weight
INDEKS KUNING TELUR (IKT) DAN Haugh Unit (HU) TELUR ITIK LOKAL DARI BEBERAPA TEMPAT BUDIDAYA ITIK DI JAWA Alfiyah, Yeni; Praseno, Koen; Mardiati, Siti Muflichatun
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 23, No 2 (2015): Volume XXIII, Nomor 2, Oktober 2015
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (520.911 KB) | DOI: 10.14710/baf.v23i2.9996

Abstract

One of the effort to increase the duck support to egg production was necessary to consideration some factors. One of the factor was feed. Parameter to determined egg quality  namely : yolk index, and haught unit (HU). IKT (Yolk Index) was a comparison between yolk and yolk diameter. Whereas HU was a quality parameter of egg freshness which was numbered based on the high of albumen and egg weight. The aim of this research was to  know the yolk index and Haugh Unit. The local egg duck from some culture place in Java and to know the management from each place as knowledge to duck farmer to optimalized  the management duck culture. The sample was got from four place culturing in Java, namely Kroya Cirebon Village (A), Pasar Bawang Brebes Village (B), Kalijoso Magelang Village (C), dan Modopuro Mojokerto Village (D), each of them 20 eggs. The Methode which used in this research was with egg measurement the parameter, such as measurement the weight duck, egg weight, albumen high, yolk hight, yolk diameter. The measurement of IKT and HU was done in structure and function of animal biology laboratory. The research design that be used was Completely Randomozed Design with SAS programme. The product of IKT and HU from four culturing duck place in Java were different, IKT has range rata (A) 0.419, (B) 0.463, (C) 0.482, and (D) 0.492. whereas the range of  HU value were A) 86.93, (B) 97.53, (C) 100.33, (D) 99.45. this was caused by the difference of management from kind of feed offering, the management that can choosed to applied for other duck farmer, such as from the duck farmer from Modopuro Mojokerto Village and Kalijoso Magelang Village.   Keywords: The Duck, Yolk Index, Haugh Unit
Kuantitas Produksi Telur Puyuh (Coturnix coturnix japonica L) Setelah Pemberian Cahaya Monokromatik Utami, Yuli Tri; Mardiati, Siti Muflichatun; Kasiyati, Kasiyati; Saraswati, Tyas Rini
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 24, No 1 (2016): Volume 24, nomor 1, tahun 2016
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.074 KB) | DOI: 10.14710/baf.v24i1.11694

Abstract

Light is one of the most important environmental aspects in poultry production. The intensity, duration, and light color are used to improve the behavior, activity, production performance, and reproduction of poultry. This study aims to determine the effect of monochromatic light used as an artificial light source in quail cultivation management. Fifty-six DOQ quails used in this study and were divided into four treatment groups. Provision of treatment in the form of lighting using monochromatic light 12 hours per day was carried out from the age of four weeks for 3 weeks. The treatment group were P0: control quails exposured with 5 W incandescent light; P1: quails exposured with 5 W red color monochromatic light; P2: quails exposured with 5 W green color monochromatic light; P3: quails exposured with 5 W blue color monochromatic light. This study was an experimental research using a completely randomized design and Duncan further test at 95% significance level. The observed parameters, namely the average of egg weight, weight of the first egg, the number of eggs (hen day egg production), sex ripe age, feed intake, and quail body weight at first laying. These results indicated that administration of monochromatic light did not affect the sex ripe age, weight of the first egg, number of eggs (hen day egg production) and water intake, but increased the egg weight, body weight, and feed intake in quail. The conclusion of this study is the provision of monochromatic light at sex ripe age doesn’t increase the number of eggs (hen day egg production).   Keywords: monochromatic light, quail eggs (Cortunix cortunix japonica L), the production of eggs.
Pengaruh Pemberian Vitamin A, B12, C dan Kombinasi Ketiganya Melalui Drinking Water Terhadap Panjang dan Bobot Tulang Femur, Tibia dan Tarsometatarsus Puyuh (Coturnix coturnix japonica L.) Setiawan, Erik; Praseno, Koen; Mardiati, Siti Muflichatun
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 21, No 1 (2013): Volume XXI, Nomor 1, Maret 2013
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.118 KB) | DOI: 10.14710/baf.v21i1.6264

Abstract

Produktivitas puyuh (Coturnix coturnix japonica L.) yang meningkat merupakan pencapaian utama dari kegiatan beternak. Hal tersebut didukung oleh proses metabolisme yang baik, sehingga untuk menjaga proses tersebut agar berjalan dengan baik maka dibutuhkan vitamin pada ransum. Vitamin adalah zat yang dibutuhkan dalam jumlah sedikit oleh tubuh yang berpengaruh terhadap kesehatan tubuh serta dalam proses pertumbuhan, perkembangan dan reproduksi. Penelitian ini bertujuan untuk mendapatkan alternatif optimalisasi perkembangan dengan manajemen pemberian suplemen pada puyuh yaitu vitamin A, B12, C dan kombinasi ketiganya melalui drinking water. Perlakuan dimulai pada saat puyuh berumur 29 hari sampai umur 63 hari. Paramater dalam penelitian ini adalah panjang dan bobot tulang femur, tibia dan tarsometatarsus. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL), seluruh data dianalisis dengan Analysis of Varian (ANOVA). Hasil analisis yang berbeda nyata diuji dengan uji lanjut Jarak Berganda Duncan pada taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa pemberian larutan vitamin A, B12, C dan kombinasi ketiganya memberikan perbedaan tidak nyata terhadap panjang dan bobot tulang femur, tibia dan tarsometatarsus. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa pemberian larutan vitamin tersebut tidak berpotensi untuk memacu pertumbuhan dan perkembangan tulang puyuh.     Kata kunci : puyuh, vitamin, panjang dan bobot tulang
Struktur Histologi Otot Femur Kelinci (Lepus Sp.) Setelah Perlakuan Implantasi Material Stainless Steel Aisi 316l Selama 2,5 Bulan Primadiani, Isabella Vanny; Mardiati, Siti Muflichatun; Tana, Silvana
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 24, No 1 (2016): Volume 24, nomor 1, tahun 2016
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (588.196 KB) | DOI: 10.14710/baf.v24i1.11690

Abstract

Production implant orthopedic domestic with 316L Stainless steel base material which use the material dimensions of the Indonesian non-import until now hasn’t been researched. Research the effect of implantation of these materials to health conditions need to be conducted on animal models, such as the rabbit before it wad applied to humans. This study aims to determine the histological changes in the femoral muscle of rabbits after implantation of AISI 316L stainless steel for 2.5 months, as part of the basic data for further research in order to figure out orthopedic implants that can be used by the body. This study compared the P0: rabbit was not given implantation of stainless steel AISI 316L with P1: Rabbits were given treatment AISI 316L stainless steel implants in the quadriceps femoris muscle parallel to the femur bone. Implantation of the implant material was conducted for 2.5 months. Histological changes observed were the presence or absence of necrosis, fibrosis and tissue cavities in the rabbit femoral muscle tissue. The results showed there were no three variables were observed. It is concluded AISI 316L stainless steel did not cause structural changes in muscle histology in a rabbit femur after implantation for 2.5 months; so that these materials can be used as an implant material in the body.   Keywords: rabbit, stainless steel AISI 316L, histology, muscle
PERTUMBUHAN PUYUH (Coturnix coturnix japonica) SETELAH PEMBERIAN TEPUNG KUNYIT (Curcuma longa L.) PADA PAKAN Widyastuti, Wuri; Mardiati, Siti Muflichatun; Saraswati, Tyas Rini
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 22, No 2 (2014): Volume XXII, NOMOR 2, OKTOBER 2014
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.007 KB) | DOI: 10.14710/baf.v22i2.7813

Abstract

Quail have the ability to grow and reproduce very quickly that at the age of 42 days. The aims of the research was to know the potential of turmeric powder as a feed additive to improve growth quail. This study used 60 quails consists of  4 treatments and 5 replications, namely P0 : as a control, a group of quail feed a standard concentrate, P1 : quail groups were given a dose of turmeric powder with 13,5 mg/quail/day, P2 : group quail were given a dose of turmeric powder with 27 mg/quail/day, and P3 : quail groups were given a dose of turmeric powder with 54 mg/quail/day. Each repeat consists of 3 quails are placed in a cage. Treatment begins at 3 weeks old quail until 2 month. Research using a completely randomized design. Parameters measured were feed consumption, water consumption, the length of the wing, the length of the femur, the length of  the tibia tarsus and the length of the tarso metatarsus. Data obtained from this study were analyzed using ANOVA ( Analysis of Varian ) at the level of 95 %. The result shows was not differences in feed consumption, water consumption, the length of the wing, the length of the femur, the length of the tibia tarsus and the length of the tarso metatarsus. Conclusion of this study  is the provision of turmeric powder in the feed does not have the potential to increase the growth of quail
KADAR KOLESTEROL TELUR PUYUH SETELAH PEMBERIAN TEPUNG KUNYIT DALAM PAKAN Aviati, Vina; Mardiati, Siti Muflichatun; Saraswati, Tyas Rini
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 22, No 1 (2014): Volume XXII, NOMOR 1, MARET 2014
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.754 KB) | DOI: 10.14710/baf.v22i1.7809

Abstract

The quail egg have complete nutrient content but its cholesterol level higher than chicken egg. This research was design to study the effect of turmeric powder supplementation to decline the cholesterol level of the quail egg. This experiment was designed by using the completely randomized design (CRD). The experimental animals were 45 female quails and   divided in to 3 groups, with 5 replications; there were P0 : as control, the group of the quail that given a standart concentrate,   P1 : the group of the quail with 54 mg/quail/day turmeric powder supplementation  before sexual maturity (from 14 days old during 1 month), and P2 : the group of quail with 54 mg/quail/day turmeric powder supplementation when sexual maturity onset (from 45 days old during 1 month). The cholesterol level of the egg sample of the quail at production in 4th month and its feed intake from each experimental group was analyzed by using analysis of variance (ANOVA) and followed by Duncan test. The result of this research showed that giving turmeric powder does not influence the feed intake and the cholesterol level of the quail egg which was given a treatment before sexual maturity, but that influence to increase the cholesterol level of quail egg that given turmeric powder when sexual maturity onset. It concluded that turmeric powder supplementation in food  not effective to decrease the cholesterol level of quail egg.
Korelasi Jumlah Folikel Ovarium dengan Konsentrasi Hormon Estrogen Mencit (Mus musculus) setelah Konsumsi Harian Tepung Kedelai selama 40 Hari Mardiati, Siti Muflichatun; Sitasiwi, Agung Janika
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 16, No 2 (2008): Vol. XVI, No. 2, Oktober 2008
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.085 KB) | DOI: 10.14710/baf.v16i2.2622

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Soybeans contains phytoestrogen that influence reproductive process. Phytoestrogen administration withchronical dosage along growth periode cause the neuroendocrine system disturbance. Commonly in humanintake of phytoestrogen from soybeans or other soybeans product. So it is important to research beneficialand adverse effects of soybeans consumption with chronical dosage to reproductive process, particulary iscorelation between the sum of ovarium follicles and the estrogen level of mouse (Mus musculus) after dailyconsumption of soybeans along 40 days. The objective of this study was to know the corelation betweenthe sum of ovarium follicle and the estrogen level of mouse, by experimental design. Adult female micewere use as experimental animal, divided into two groups, there were control and treatment. Thegroup of treatment was administrated with 0,975 mg soybeans flour, it was contain ± 0,00156 mgphytoestrogen. The control group was administreted only drinking water. The result of this study showsthat were not significantly difference between control and treatment group. It concluded the corelationbetween the sum of ovarium follicles and the estrogen level were not influenced with daily consumption offitoestrogen.