KIKIN HAMZAH MUTAQIN
Department Of Plant Protection, Faculty Of Agriculture, IPB University

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Geminiviruses Associated with the Weed Species Ageratum conyzoides, Centipeda minima, Porophyllum ruderale, and Spilanthes iabadicensis from Java, Indonesia RIKA MELIANSYAH; SR HENDRASTUTI HIDAYAT; KIKIN HAMZAH MUTAQIN
Microbiology Indonesia Vol. 5 No. 3 (2011): September 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.939 KB) | DOI: 10.5454/mi.5.3.4

Abstract

Geminivirus has a wide host range including cultivated plants and weeds. Infected weeds may play an important role in disease epidemic. Unfortunately, little is known about weeds species that may serve as alternative host for Geminivirus. This research was conducted to identify Geminivirus on weeds around chili pepper field to study their potential role as virus reservoir. Field surveys were conducted to chilli pepper growing area inWest and Central Java Provinces, and The Special Province of Yogyakarta during 2009 to collect symptomatic weed plants. Geminivirus infection was detected using PCR technique from 9 weed samples, i.e. 5 samples Ageratum conyzoides from Bogor (AgrBgr), Sukabumi (AgrSkm), Magelang (AgrMgl), Sleman (AgrJgy), and Garut (AgrGrt); Centipeda minima from Magelang (CtpMgl); A. boehmerioides from Sleman (AcpJgy); Porophyllum ruderale from Bogor (PrlBgr); Spilanthes iabadicensis from Magelang (SplMgl). Further genetic analysis showed that those geminiviruses can be differentiated into 2 clusters, showing the possible genetic differences among them. They neither have a close relationship with other geminiviruses published earlier in the GenBank, indicating weed infecting collected Geminivirus in this study is possibly a distinct Geminivirus.
Identification of mealybugs on Piper nigrum as vector of Piper yellow mottle virus (Badnavirus: Caulimoviridae) Miftakhurohmah Miftakhurohmah; Sri Hendrastuti Hidayat; Kikin Hamzah Mutaqin; Bonny Poernomo Wahyu Soekarno; Dono Wahyuno
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 22 No. 2 (2022): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.222144-153

Abstract

Piper yellow mottle virus (PYMoV) is the dominant virus that causes mottle disease in black pepper (Piper nigrum). Two species of mealybugs, Ferrisia virgata and Planococcus minor have been reported as vectors of PYMoV. A different species of mealybug that has never been reported before was found in black pepper. Molecular approaches including total DNA isolation of mealybug collected from the field, mealybugs identification by DNA barcode, detection of PYMoV in single mealybugs, were conducted as an approach to identify the potential of mealybugs as PYMoV vector in the field. Mealybugs were collected from black pepper plants in Cimanggu (Bogor, West Java) and Sukamulya (Sukabumi, West Java). Characters of adult females were observed for morphological identification. Molecular-based identification of the mealybugs and PYMoV involved the following procedures: total DNA isolation, DNA amplification, nucleotide sequencing and sequence analysis. Three species of mealybugs, P. minor, F. virgata and Paracoccus marginatus were confirmed by morphological and molecular identification. This is the first report for the occurrence of P. marginatus in black pepper plants. PYMoV was successfully detected from field samples of F. virgata, P. minor and P. marginatus. This finding indicates the potential of insect vectors for disease spread and distribution.